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The map label for this gene is tmk

Identifier: 116516561

GI number: 116516561

Start: 843793

End: 844431

Strand: Direct

Name: tmk

Synonym: SPD_0825

Alternate gene names: 116516561

Gene position: 843793-844431 (Clockwise)

Preceding gene: 116516457

Following gene: 116515715

Centisome position: 41.24

GC content: 45.7

Gene sequence:

>639_bases
ATGTCAAAAGGATTTTTAGTCTCTCTTGAGGGACCAGAGGGAGCAGGCAAGACCAGTGTTTTAGAGGCTCTGCTACCAAT
TTTAGAGGAAAAAGGAGTAGAGGTGCTGACGACCCGTGAACCTGGCGGAGTCTTGATTGGGGAGAAGATTCGGGAAGTGA
TTTTGGATCCAAGTCATACTCAGATGGATGCTAAAACAGAGCTACTTCTCTATATTGCCAGTCGCAGACAGCATTTGGTG
GAAAAAGTTCTTCCAGCCCTTGAAGCTGGCAAGTTGGTCATCATGGATCGTTTTATCGATAGTTCTGTTGCCTATCAGGG
ATTTGGTCGTGGCTTAGATATTGAAGCCATTGACTGGCTCAATCAGTTTGCGACAGATGGCCTCAAACCCGATTTGACAC
TCTATTTTGACATCGAGGTGGAAGAAGGGCTGGCTCGTATTGCTGCTAATAGTGACCGCGAGGTTAATCGTTTGGATTTG
GAAGGGTTGGACTTGCATAAAAAAGTTCGTCAAGGCTACCTTTCTCTTCTGGATAAAGAGGGAAATCGCATTGTCAAGAT
TGATGCTAGTCTCCCTTTGGAGCAAGTTGTGGAAACTACCAATGCTGTCTTGTTTGACGGAATGGGCTTGGCCAAATGA

Upstream 100 bases:

>100_bases
GCCTCTTTTATGGTGGCTGAAATGAGAAGACACAAAAAGATTGTCACAAACTCCTATTTTTTTGATAGAATAGAAGTAGT
AAAAAAGAAATGAGTTAGAC

Downstream 100 bases:

>100_bases
AACAAGATCAACTAAAGGCTTGGCAACCAGCTCAGTTTGACCGTTTTGTCCGTATCTTAGAACAAGACCAGCTCAATCAC
GCCTATCTCTTTTCAGGTTT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLV
EKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK

Sequences:

>Translated_212_residues
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLV
EKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK
>Mature_211_residues
SKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVE
KVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLE
GLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=203, Percent_Identity=39.4088669950739, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322517, Length=201, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_STRP2 (Q04KZ0)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816308.1
- ProteinModelPortal:   Q04KZ0
- STRING:   Q04KZ0
- EnsemblBacteria:   EBSTRT00000019479
- GeneID:   4441266
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0825
- eggNOG:   COG0125
- GeneTree:   EBGT00050000028362
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23339; Mature: 23208

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHT
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCCCC
QMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHH
NQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLEGLDLHKKVRQGYLSLLDKE
HHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCC
GNRIVKIDASLPLEQVVETTNAVLFDGMGLAK
CCEEEEEECCCCHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
SKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGVLIGEKIREVILDPSHT
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCCCC
QMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSSVAYQGFGRGLDIEAIDWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHH
NQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDLEGLDLHKKVRQGYLSLLDKE
HHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCC
GNRIVKIDASLPLEQVVETTNAVLFDGMGLAK
CCEEEEEECCCCHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA