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The map label for this gene is holB [H]

Identifier: 116515715

GI number: 116515715

Start: 844428

End: 845318

Strand: Direct

Name: holB [H]

Synonym: SPD_0826

Alternate gene names: 116515715

Gene position: 844428-845318 (Clockwise)

Preceding gene: 116516561

Following gene: 116516332

Centisome position: 41.27

GC content: 41.3

Gene sequence:

>891_bases
ATGAAACAAGATCAACTAAAGGCTTGGCAACCAGCTCAGTTTGACCGTTTTGTCCGTATCTTAGAACAAGACCAGCTCAA
TCACGCCTATCTCTTTTCAGGTTTCTTTGGAAGCTTGGAAATGGCGCAATTTTTAGCTAAGAGCCTTTTTTGTACGGATA
AAGTTGGCGTCTTACCATGTGAGAAATGCCGAAGTTGCAAGCTGATTGAACAGGAAGAGTTTCCAGATGTCACCTTGATT
AAGCCAGTCAATCAGGTCATCAAGACAGAACGCATTCGGGAATTGGTGGGACAGTTTTCTCAAGCAGGGATTGAAAGCCA
GCAACAGGTCTTTATCATCGAGCAAGCGGATAAAATGCATCCCAACGCAGCCAATTCTCTGCTCAAGGTCATCGAAGAAC
CCCAGAGTGAAGTTTATATTTTCTTCTTGACTAGCGATGAGGAAAAGATGTTACCGACAATCCGAAGTCGGACTCAGATC
TTCCACTTTAAAAAGCAAGAAGAAAAACTTATCTTACTCTTAGAACAAATGGGACTTGTTAAGAAAAAAGCGACTCTTTT
AGCTAAGTTTAGTCAATCGCGAGCTGAAGCAGAAAAGTTGGCACATCAGGCAAGTTTTTGGACCTTGGTCGATGAAAGTG
AACGCCTGCTGACTTGGTTAGTAGCTAAGAAAAAAGAAAGTTATCTACAGGTTGCCAAATTAGCCAACTTGGCAGATGAT
AAGGAAAAACAGGATCAGGTTTTACGGATTCTTGAAGTTCTCTGTGGGCAGGACCTCTTGCAGGTAAGAGTAAGAGTGAT
TCTACAAGATTTACTAGAAGCTAGAAAAATGTGGCAAGCTAATGTCAGCTTTCAAAATGCCATGGAATATCTGGTCTTGA
AAGAAATATAA

Upstream 100 bases:

>100_bases
AAGAGGGAAATCGCATTGTCAAGATTGATGCTAGTCTCCCTTTGGAGCAAGTTGTGGAAACTACCAATGCTGTCTTGTTT
GACGGAATGGGCTTGGCCAA

Downstream 100 bases:

>100_bases
ACTCAAAAATGAATGATAAAGAAAGGAAAGGGCTGTTTTATGGACAAAAAAGAATTATTTGACGCGCTGGATGATTTTTC
CCAACAGTTATTGGTAACCT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQFLAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLI
KPVNQVIKTERIRELVGQFSQAGIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLTWLVAKKKESYLQVAKLANLADD
KEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARKMWQANVSFQNAMEYLVLKEI

Sequences:

>Translated_296_residues
MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQFLAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLI
KPVNQVIKTERIRELVGQFSQAGIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLTWLVAKKKESYLQVAKLANLADD
KEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARKMWQANVSFQNAMEYLVLKEI
>Mature_296_residues
MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQFLAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLI
KPVNQVIKTERIRELVGQFSQAGIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLTWLVAKKKESYLQVAKLANLADD
KEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARKMWQANVSFQNAMEYLVLKEI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=170, Percent_Identity=31.7647058823529, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787341, Length=165, Percent_Identity=26.0606060606061, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34382; Mature: 34382

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQFLAKSLFCTDKVGVLPC
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQAGIESQQQVFIIEQADKMH
HHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQIFHFKKQEEKLILLLEQMGLV
CHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLTWLVAKKKESYLQVAKLANLADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARKMWQANVSFQNAMEYLVLKEI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQFLAKSLFCTDKVGVLPC
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQAGIESQQQVFIIEQADKMH
HHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQIFHFKKQEEKLILLLEQMGLV
CHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLTWLVAKKKESYLQVAKLANLADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARKMWQANVSFQNAMEYLVLKEI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]