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The map label for this gene is thyA [H]

Identifier: 116516193

GI number: 116516193

Start: 608738

End: 609577

Strand: Direct

Name: thyA [H]

Synonym: SPD_0581

Alternate gene names: 116516193

Gene position: 608738-609577 (Clockwise)

Preceding gene: 116517028

Following gene: 116515353

Centisome position: 29.75

GC content: 43.33

Gene sequence:

>840_bases
ATGACAAAAGCAGATACGATTTTTAAAGAGAATATTGAACGCATTCTAAAGGAAGGTGTTTTCTCAGAGCAGGCTCGTCC
TAAGTACAAGGATGGGACTGTTGCTAATTCCAAGTATGTAACTGGTGCCTTTTCCGAGTACGACTTATCTAAAGGAGAAT
TTCCTATCACAACTTTGCGTCCCATTGCAATCAAATCCGCCATCAAAGAAGTTCTCTGGATTTACCAAGACCAGTCAAAT
AGCTTAGAAGTGCTTAATGACAAGTACAATGTTCACTACTGGAATGACTGGGAAGTTGGAGACACGGGAACCATTGGTGA
GCGCTATGGTGCCGTTGTTAAGAAACACGACATTATCAATAAGCTTCTCAAACAGTTGGAAACCAATCCTTGGAACCGCC
GCAATATTATTTCGCTCTGGGATTACCAAGCTTTCGAAGAAACAGATGGGCTGCTCCCGTGCGCCTTTCAGACCATGTTT
GATGTTCGGCGTGTTGATGGGGAAATCTATCTGGATGCGACCTTGACCCAGCGCTCCAATGATATGCTGGTGGCCCACCA
CATCAACGCTATGCAGTATGTGGCTTTGCAGATGATGATTGCCAAACATTTTGGCTGGAAGGTTGGGAAGTTCTTCTACT
TCATCAACAACCTCCATATCTATGATAATCAATTTGAACAAGCTCAGGAATTGCTCCGTCGGGAGCCGTCAAACTGCCAA
CCACGCTTGGTTTTAAATGTTCCTGATGGGACTAATTTCTTTGATATCAAAGCAGAAGATTTTGAGTTGGTGGATTATGA
CCCTGTTAAGCCACAGTTGAAGTTTGACCTAGCTATTTAA

Upstream 100 bases:

>100_bases
ATAAGATAATGAGAGAGGAAAATGCTAACCTAGAGTTAGTAATGTTCCTCTTTTATTTTTTATGCTATACTAGTTAGGAC
AATGAATGAGGTGAAACGAA

Downstream 100 bases:

>100_bases
AAGAATAGAAAAAAGAAGTTGAGAATAATCCCAACTTCTTTTGTTTCTTAACGTGATACGCGGCGACGAGCTGCTTTTTT
ACGGTTTTCTTCGATGAAAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSN
SLEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQ
PRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI

Sequences:

>Translated_279_residues
MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSN
SLEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQ
PRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI
>Mature_278_residues
TKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNS
LEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFD
VRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQP
RLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=296, Percent_Identity=27.7027027027027, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1789191, Length=262, Percent_Identity=31.6793893129771, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71993377, Length=302, Percent_Identity=26.158940397351, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324648, Length=204, Percent_Identity=27.9411764705882, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137556, Length=276, Percent_Identity=28.2608695652174, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32554; Mature: 32423

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKHDIIN
HHHHHHHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFDVRRVDGEIYLDATLTQRSN
HHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
DMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQ
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECEEEECCHHHHHHHHHHCCCCCCC
PRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI
CEEEEECCCCCCEEEEECCCEEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
TKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKHDIIN
HHHHHHHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFDVRRVDGEIYLDATLTQRSN
HHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
DMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQ
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECEEEECCHHHHHHHHHHCCCCCCC
PRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI
CEEEEECCCCCCEEEEECCCEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA