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The map label for this gene is miaA

Identifier: 116515353

GI number: 116515353

Start: 609916

End: 610800

Strand: Direct

Name: miaA

Synonym: SPD_0583

Alternate gene names: 116515353

Gene position: 609916-610800 (Clockwise)

Preceding gene: 116516193

Following gene: 116517076

Centisome position: 29.81

GC content: 45.42

Gene sequence:

>885_bases
ATGAAAACAAAAATAATTGTGATTGTTGGACCGACTGCTGTTGGAAAGACGGCTCTAGCTATTGAAGTTGCAAAGCGCTT
TAATGGCGAAGTGGTTAGTGGAGATAGCCAGCAAGTCTATCGAGGACTTGATATTGGGACGGCCAAGGCTAGTCCAGAAG
AGCAGGCAGCTGTTCCTCATCATTTAATCGATGTTAGAGAGATAACCGAGTCTTACTCGGCTTTTGATTTTGTTTCAGAA
GCTAAGATGACTATTGAGGATATTCACAGCCGTGGCAAGCTAGCCATTATCGCCGGTGGGACTGGACTTTATATCCAGAG
CTTGCTAGAAGGTTACCACCTAGGTGGGGAGACTCCTCATGAGGAGATTTTAGCTTATCGAGCTAGTTTGGAGCCATATT
CAGATGAGGAATTAGCCCATTTGGTGGAGCAAGCAGGCCTTGAGATTCCCCAGTTTAATCGTCGTCGTGCTATGCGTGCC
TTAGAAATTGCCCATTTTGGTCAGGATTTGGAAAATCAAGAGATTTTGTATGAACCGCTGATTATCTGCTTGGATGATGA
ACGTAGTCAACTTTATGAGCGTATCAACCACCGAGTGGATTTGATGTTTGAGGCTGGGCTTTTGGATGAGGCCAAGTGGC
TGTTTGACCATTCCCCTAATGTACAGGCTGCTAAAGGAATTGGCTATAAGGAACTCTTTCCTTATTTCCGTGGAGAGCAG
ACCTTTGAGGAAGCTCGTGAGAGTCTTAAACAGGCGACCCGTCGTTTTGCCAAGCGTCAGCTGACCTGGTTCCGTAATCG
CATGCAGGTCACCTTTTATCAGATTGGAGAATCTGGTGTACAAGACCGCATTTTAAGCCAGATAGAGGAGTTTTTAGATG
ATTGA

Upstream 100 bases:

>100_bases
CTCAATCAGCCAGCCTCCTCAAGAGGTCACATTTTTCTGACTGACCTTTTTGTGTTATAATAATAGTAACGAAAAAATGG
GAATTTTTCAAGGAAAAAAG

Downstream 100 bases:

>100_bases
AACGGAGAAAAAAGAGGAGCGAGTCCTGCTGATTGGTGTGGAATTGCAGGGTATGGACAGTTTTGACCTCTCCATGGAAG
AATTGGCTAGTTTAGCGAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSE
AKMTIEDIHSRGKLAIIAGGTGLYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQ
TFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD

Sequences:

>Translated_294_residues
MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSE
AKMTIEDIHSRGKLAIIAGGTGLYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQ
TFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD
>Mature_294_residues
MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSE
AKMTIEDIHSRGKLAIIAGGTGLYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQ
TFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=31.6129032258064, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1790613, Length=283, Percent_Identity=34.2756183745583, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=27.6666666666667, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=31.8493150684932, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24649830, Length=338, Percent_Identity=31.9526627218935, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_STRP2 (Q04LL6)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816082.1
- ProteinModelPortal:   Q04LL6
- SMR:   Q04LL6
- STRING:   Q04LL6
- EnsemblBacteria:   EBSTRT00000020125
- GeneID:   4441153
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0583
- eggNOG:   COG0324
- GeneTree:   EBGT00050000027823
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33449; Mature: 33449

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPH
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHCCCCCCCCCCCHHHHCCCH
HLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTGLYIQSLLEGYHLGGETPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCH
EEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRALEIAHFGQDLENQEILYEPL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCE
IICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQ
EEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCH
TFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPH
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHCCCCCCCCCCCHHHHCCCH
HLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTGLYIQSLLEGYHLGGETPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCH
EEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRALEIAHFGQDLENQEILYEPL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCE
IICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQ
EEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCH
TFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA