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The map label for this gene is sorB [H]

Identifier: 116516149

GI number: 116516149

Start: 67629

End: 68105

Strand: Direct

Name: sorB [H]

Synonym: SPD_0066

Alternate gene names: 116516149

Gene position: 67629-68105 (Clockwise)

Preceding gene: 116517213

Following gene: 116515606

Centisome position: 3.31

GC content: 41.09

Gene sequence:

>477_bases
ATGACAATTGTAGGATGCCGTATTGATGGACGTTTGATCCACGGACAAGTAGCCAATCTTTGGGCTGGAAAACTAAATGT
TTCACGCATTATGGTTGTAGACGACGAAGTTGTCAACAACGATATTGAAAAGAGTGGTTTGAAACTTGCGACACCACCAG
GTGTGAAATTGAGTATTTTGCCAGTTGAGAAAGCTGCAGCCAATATTCTTGCTGGTAAATACGATAGCCAACGTCTCTTT
ATCGTGGCTCGTAAACCAGACCGCTTCCTTGGTTTGGTAGAAGCAGGTGTACCACTTGAAACCCTTAATGTTGGGAATAT
GTCTCAAACACCAGAAACTCGTTCTATTACACGTTCTATCAACGTAGTAGACAAGGATGTGGAAGACTTCCACAAACTGG
CAGAAAAAGGTGTTAAACTTACTGCTCAGATGGTTCCAAATGATCCAATTTCAGACTTTTTGAGCTTATTAAAATAG

Upstream 100 bases:

>100_bases
GTGCCAACCGTATCATTATCTTTGAAACAGAAGGTCAATATAAAGAAGAGATTCATTTAACTCGTAAACCTACACTAAAA
CATATAAAGGGGGAAAACTT

Downstream 100 bases:

>100_bases
GAAAAAAATTTTTAGGAGGTCATTGTTATGATACAATGGTGGCAAATTTTACTTCTCACTTTGTACTCAGCTTATCAAAT
CTGTGATGAGTTGACGATCG

Product: PTS system, IIB component

Products: NA

Alternate protein names: EIIB-Sor; EIII-B-Sor; PTS system sorbose-specific EIIB component [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLF
IVARKPDRFLGLVEAGVPLETLNVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK

Sequences:

>Translated_158_residues
MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLF
IVARKPDRFLGLVEAGVPLETLNVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK
>Mature_157_residues
TIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFI
VARKPDRFLGLVEAGVPLETLNVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=157, Percent_Identity=36.9426751592357, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI87082217, Length=155, Percent_Identity=33.5483870967742, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1789527, Length=147, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=8e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17228; Mature: 17097

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSIL
CEEEEEEECCEEEECCHHHEEECCCCCEEEEEECCHHHCCCHHHCCCEEECCCCCEEEEE
PVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETLNVGNMSQTPETRSITRSI
EHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEECCCCCEEEECCCCCCCCCCHHHHHHHH
NVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK
HHCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHC
>Mature Secondary Structure 
TIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSIL
EEEEEEECCEEEECCHHHEEECCCCCEEEEEECCHHHCCCHHHCCCEEECCCCCEEEEE
PVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETLNVGNMSQTPETRSITRSI
EHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEECCCCCEEEECCCCCCCCCCHHHHHHHH
NVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK
HHCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7947968 [H]