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The map label for this gene is pdhC [H]

Identifier: 116515407

GI number: 116515407

Start: 1051554

End: 1052597

Strand: Reverse

Name: pdhC [H]

Synonym: SPD_1026

Alternate gene names: 116515407

Gene position: 1052597-1051554 (Counterclockwise)

Preceding gene: 116516741

Following gene: 116515652

Centisome position: 51.44

GC content: 42.43

Gene sequence:

>1044_bases
ATGGCTGATGACAAGCTAAGAGCGACTCCTGCAGCTAGAAAGTTAGCGGATGATTTAGGGATAAACCTCTACGACGTTTC
TGGCTCAGGTGCAAACGGTCGTGTCCACAAAGAAGACGTGGAAACTTATAAAGACACAAACGTGGTTCGCATTTCGCCAC
TTGCAAAACGAATTGCCCTCGAACATAACATTGCTTGGCAGGAAATCCAAGGAACCGGTCATCGTGGTAAAATCATGAAG
AAGGATGTTTTGGCCCTGCTTCCTGAAAATATCGAAAACGATAGCATCAAATCTCCTGCTCAGATTGAAAAAGTGGAAGA
AGTCCCTGATAACGTAACACCATATGGTAAAATCGAGCGTATTCCAATGACACCAATGCGTAAGGTTATTGCCCAACGCA
TGGTTGAATCTTACTTGACTGCGCCAACCTTCACCCTCAACTATGAAGTTGATATGACTGAAATGTTGGCTCTTCGTAAG
AAGGTTCTTGAGCCAATCATGGAAGCAACTGGGAAGAAGACTACTGTAACAGACCTTCTTTCGCTTGCAGTTGTTAAGAC
TCTTATGAAACACCCATACATCAACGCTTCATTGACAGAAGATGGCAAGACTATTATCACTCACAACTATGTCAATCTTG
CCATGGCAGTTGGGATGGATAATGGATTGATGACACCTGTTGTTTACAATGCTGAGAAGATGAGTCTTTCAGAACTGGTT
GTAGCCTTTAAGGATGTTATTGGCCGTACCTTGGATGGTAAATTGGCTCCAAGTGAGTTACAAAATTCAACATTCACAAT
CAGTAATTTGGGAATGTTTGGTGTTCAGTCCTTTGGTCCGATTATTAACCAACCCAACTCAGCTATCCTTGGTGTCAGTT
CGACAATCGAGAAGCCAGTTGTCGTCAATGGTGAAATTGTGATTCGCCCAATCATGAGTTTAGGATTAACCATTGACCAC
CGTGTCGTAGATGGTATGGCTGGTGCTAAGTTTATGAAGGACTTGAAAGAGTTGATTGAAACGCCAATCTCAATGTTAAT
TTAA

Upstream 100 bases:

>100_bases
AAAAAGATAAATCTCCTTGGCTTCATCGAAGCCATCGTCGATTTCCTATTTTTCAGTCGCTTTTGACGCCCTTTGTATCA
TAAATAGAATTGGAGAGGTC

Downstream 100 bases:

>100_bases
GAGCAAGAGTATTCATTTTTAAGATATAGATAAAGGAAGGAAGACATGGCCTTAGAAGTAATTATGCCAAAAGCCGGCGT
GGATATGACAGAAGGACAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYKDTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMK
KDVLALLPENIENDSIKSPAQIEKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVGMDNGLMTPVVYNAEKMSLSELV
VAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQSFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDH
RVVDGMAGAKFMKDLKELIETPISMLI

Sequences:

>Translated_347_residues
MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYKDTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMK
KDVLALLPENIENDSIKSPAQIEKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVGMDNGLMTPVVYNAEKMSLSELV
VAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQSFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDH
RVVDGMAGAKFMKDLKELIETPISMLI
>Mature_346_residues
ADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYKDTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKK
DVLALLPENIENDSIKSPAQIEKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRKK
VLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVGMDNGLMTPVVYNAEKMSLSELVV
AFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQSFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHR
VVDGMAGAKFMKDLKELIETPISMLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=303, Percent_Identity=35.3135313531353, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=31.5789473684211, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI203098753, Length=327, Percent_Identity=28.7461773700306, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=327, Percent_Identity=28.7461773700306, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI110671329, Length=312, Percent_Identity=28.8461538461538, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI260898739, Length=183, Percent_Identity=30.6010928961749, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1786946, Length=297, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786305, Length=293, Percent_Identity=33.1058020477816, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=246, Percent_Identity=34.5528455284553, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=311, Percent_Identity=30.5466237942122, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=27.741935483871, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17537937, Length=312, Percent_Identity=25.9615384615385, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=250, Percent_Identity=39.2, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=312, Percent_Identity=32.6923076923077, Blast_Score=144, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=34.375, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24582497, Length=306, Percent_Identity=30.0653594771242, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI20129315, Length=306, Percent_Identity=30.0653594771242, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859875, Length=306, Percent_Identity=25.8169934640523, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 38099; Mature: 37968

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYKDTNVVRISPLAKRIAL
CCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHCCCCEEEECHHHHHHHH
EHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQIEKVEEVPDNVTPYGKIER
HCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEC
IPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRKKVLEPIMEATGKKTTVTDLL
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVGMDNGLMTPVVYNAEKMSLSELV
HHHHHHHHHHCCCCCCEECCCCCEEEEECCEEEEEEECCCCCCCCCEEECCHHHHHHHHH
VAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQSFGPIINQPNSAILGVSSTIEKPV
HHHHHHHCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHCHHHCCCCCEEEECHHHCCCCE
VVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKDLKELIETPISMLI
EECCCCCEEEHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYKDTNVVRISPLAKRIAL
CCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHCCCCEEEECHHHHHHHH
EHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQIEKVEEVPDNVTPYGKIER
HCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEC
IPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRKKVLEPIMEATGKKTTVTDLL
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVGMDNGLMTPVVYNAEKMSLSELV
HHHHHHHHHHCCCCCCEECCCCCEEEEECCEEEEEEECCCCCCCCCEEECCHHHHHHHHH
VAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQSFGPIINQPNSAILGVSSTIEKPV
HHHHHHHCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHCHHHCCCCCEEEECHHHCCCCE
VVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKDLKELIETPISMLI
EECCCCCEEEHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]