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The map label for this gene is lpdA [C]

Identifier: 116515652

GI number: 116515652

Start: 1049805

End: 1051490

Strand: Reverse

Name: lpdA [C]

Synonym: SPD_1025

Alternate gene names: 116515652

Gene position: 1051490-1049805 (Counterclockwise)

Preceding gene: 116515407

Following gene: 116516751

Centisome position: 51.39

GC content: 42.76

Gene sequence:

>1686_bases
ATGCCAAAAGCCGGCGTGGATATGACAGAAGGACAAATCGTCCAATGGAATAAAAAAGTCGGAGAATTTGTAAAAGAAGG
AGAAATCCTTTTGGAAATCATGACTGATAAAGTCAGCATGGAATTGGAAGCCGAAGAAGATGGGTACTTGATTGCCATTC
TCAAAGGAGATGGTGAAACTGTCCCTGTAACGGAAGTTATCGGTTACCTTGGTGAAGAAAGGGAAAACATCCCAACAGCT
GGAGCAGCATCGCCAGAAGCTAGCCCTGTACCTGTAGCAAGTACCTCAAACGATGATGGTAAGAGCGATGATGCTTTTGA
TATCGTTGTGATTGGTGGAGGTCCTGCTGGTTATGTTGCAGCCATTAAAGCTGCCCAATTCGGCGGTAAGGTTGCCCTTG
TTGAGAAATCTGAACTTGGTGGAACCTGCTTAAACCGTGGATGTATTCCAACCAAGACCTACCTTCATAACGCTGAAATT
ATTGAAAATATCGGTCATGCTGCAAATCGTGGTATCGTGATTGAAAATCCTAACTTCACTGTTGATATGGAAAAACTTTT
AGAAACTAAATCTAAAGTTGTTAATACTCTTGTTGGTGGTGTTGCAGGACTTCTTCGTAGCTACGGAGTTACTGTTCATA
AAGGAATTGGTACAATCACTAAAGACAAGAACGTCTTGGTAAATGGTTCTGAATTGCTTGAAACCAAGAAAATCATTCTT
GCTGGTGGTTCAAAAGTCAGCAAGATCAACGTCCCTGGTATGGAATCTCCACTTGTCATGACTAGTGATGACATTCTTGA
AATGAACGAAGTGCCAGAAAGCCTTGTTATCATCGGTGGTGGAGTTGTCGGTATCGAACTCGGTCAGGCCTTCATGACAT
TTGGTTCAAAAGTGACTGTTATCGAAATGATGGACCGTATAGTTCCAGCTATGGATGTGGAAGTTTCTAAGAACCTTCGC
TTGATCCTTGAGCGTAAAGGAATGACCATCTTGACTGGTACTAAACTGCAAGAAATCATTGAGGAAAATGGTCAACTTCG
TATCAAGGTTGAAGGAAAAGACGATATCATCGCAAGCAAAGCTCTTCTTTCAATTGGTCGTATGCCAGACCTTGAAGGTA
TTGGAGAGGTTGAGTTTGAATTGGATCGTGGTTGTATCAAGGTCAACGAATACATGGAAACTTCAGTTCCAGGCATTTAT
GCACCAGGTGACATCAACGGTACTAAGATGTTGGCTCACGCAGCTTTCCGCATGGGTGAAGTTTCCGCTGAAAATGCCCT
TAAAGGAAATCATGCAGTTGCCAAATTGAATTTGACTCCTGCAGCCATCTACACTCTCCCTGAAGTAGCAGCAGTAGGTT
TGACAGAAGAACAAGCCCGTGAGAAATACGATGTTGCCATCGGTAAGTTTAACTTTGCTGCTAACGGTCGTGCTATTGCA
TCTGACGCAGCTCAAGGTTTCGTAAAAGTTATCGCTGATAAGAAATACGGAGAAATCCTTGGTGTACACATTATTGGTCC
TGCAGCCGCAGAATTAATAAACGAAGCATCAAGCATCATCGAAATGGAAATCACTGTTGAGGAAATGCTGAAGACCATCC
ACGGGCACCCAACTTACTCTGAAGTGATGTACGAAGCGTTTGCAGATGTTCTAGGAATGGCCATCCATTCACCTAAGAAA
AAATAA

Upstream 100 bases:

>100_bases
AGAGTTGATTGAAACGCCAATCTCAATGTTAATTTAAGAGCAAGAGTATTCATTTTTAAGATATAGATAAAGGAAGGAAG
ACATGGCCTTAGAAGTAATT

Downstream 100 bases:

>100_bases
AACGAAGATAGATTAGACTGGAAGGTTATTTCCAGTTTCTATCATATAAAGTAAAGGGATATCATGAAATACATTATCAA
TCATTCAAATGACACTGCTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTA
GAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLETKKIIL
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLR
LILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIY
APGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
K

Sequences:

>Translated_561_residues
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTA
GAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLETKKIIL
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLR
LILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIY
APGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
K
>Mature_560_residues
PKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAG
AASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEII
ENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLETKKIILA
GGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRL
ILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYA
PGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIAS
DAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=38.5106382978723, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=26.6958424507659, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=29.1304347826087, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277065, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI148277071, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=24.7113163972286, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=23.8938053097345, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=38.1696428571429, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=27.8867102396514, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=28.0434782608696, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=27.6744186046512, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789065, Length=294, Percent_Identity=24.8299319727891, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=39.5348837209302, Blast_Score=286, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17557007, Length=487, Percent_Identity=27.1047227926078, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=450, Percent_Identity=27.5555555555556, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=27.5555555555556, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=24.4769874476987, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=38.2231404958678, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=28.9640591966173, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.0777537796976, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=79, Percent_Identity=39.2405063291139, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=42.2657952069717, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=26.3713080168776, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=24.9500998003992, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=24.6346555323591, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=24.5161290322581, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 59760; Mature: 59629

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGET
CCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEEECCCCE
VPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVA
ECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
AIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEIIENIGHAANRGIVIENPNFT
HHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHCCHHHHHHHCHHHCCCEEEECCCCE
VDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLETKKIIL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCEEECCHHHHCEEEEEE
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTV
ECCCEEEEEECCCCCCCEEECCCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHCCCCCHH
IEMMDRIVPAMDVEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASK
HHHHHHHCCCCCEEECCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHH
ALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGE
HHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
VSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
CCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECCCCCEEH
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYS
HHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHCCCCCHH
EVMYEAFADVLGMAIHSPKKK
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGET
CCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEEECCCCE
VPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVA
ECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
AIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEIIENIGHAANRGIVIENPNFT
HHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHCCHHHHHHHCHHHCCCEEEECCCCE
VDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLETKKIIL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCEEECCHHHHCEEEEEE
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTV
ECCCEEEEEECCCCCCCEEECCCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHCCCCCHH
IEMMDRIVPAMDVEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASK
HHHHHHHCCCCCEEECCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHH
ALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGE
HHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
VSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
CCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECCCCCEEH
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYS
HHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHCCCCCHH
EVMYEAFADVLGMAIHSPKKK
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2832161; 2404760; 1880807 [H]