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The map label for this gene is pdhB [H]

Identifier: 116516741

GI number: 116516741

Start: 1052815

End: 1053807

Strand: Reverse

Name: pdhB [H]

Synonym: SPD_1027

Alternate gene names: 116516741

Gene position: 1053807-1052815 (Counterclockwise)

Preceding gene: 116516204

Following gene: 116515407

Centisome position: 51.5

GC content: 44.01

Gene sequence:

>993_bases
ATGGAAACAAAAACAATGTCCTTCCGTGACACCATTATCCTTGCTATGTCTGAGGAAATGCGTCGCGATGAAAATGTGTT
CTTGATGGGAGAAGATGTCGGTGTCTTCGGAGGAGACTTCGGTACTTCTGTTGGAATGCTTGAAGAATTTGGTCCAGAAC
GTGTCCGTGACTGTCCGATTTCTGAAGCTGCCATCTCTGGAGCAGCAGCAGGAGCAGCCATGACAGGACTTCGTCCAATC
GTCGATATGACCTTCATGGACTTCTCTGTTATTGCCATGGACAATATCGTCAACCAAGCTGCTAAAACACGTTACATGTT
TGGTGGTAAAGGTCAGGTTCCAATGACTGTTCGTTGTGCAGCTGGTAACGGAGTTGGCTCTGCCGCCCAGCACTCACAAT
CTCTAGAGTCTTGGTTTACTCACATTCCAGGACTTAAGGTTGTGGCACCTGGAACACCTGCGGACATGAAAGGCCTGCTT
AAGTCTTCTATCCGTGATAACAACCCAGTTATTATACTTGAGTACAAGTCAGAATTTAACCAAAAAGGGGAAGTGCCAGT
TGATCCAGACTACACAATTCCACTTGGGGTTGGTGAAATCAAACGCCAAGGAACGGATGTAACAGTTGTTACTTATGGAA
AAATGCTTCGCCGTGTGGTTCAAGCTGCTGAAGAATTAGCAGAAGAAGGAATTTCAGTTGAAATTGTTGACCCACGTACC
CTTGTTCCGCTTGATAAGGATATCATCATAAACTCAGTGAAGAAGACTGGTAAGGTTATTCTGGTCAACGATGCCCACAA
AACAAGTGGCTATATCGGTGAAATTTCAGCTATTATTTCAGAATCAGAAGCATTTGACTATCTAGATGCACCAATACGCC
GTTGTGCAGGAGAAGATGTGCCGATGCCTTATGCACAAAACCTAGAAAATGCAATGATTCCAACAGTTGAAAGTATCAAA
GATGCAATCCGAAAAACTTATAACAAAGACTAG

Upstream 100 bases:

>100_bases
AGCAGTAGAAGCTTCTGTTAAATTTGCAGAGGAAAGCCCATTCCCACCGCTTGAATCAGCCTTTGAAGATATTTACACAG
ACTAAGGAGAAAGAGATAAA

Downstream 100 bases:

>100_bases
AGTTACATAATATAAGTTATGTAAATAAAATAAAGCTGACGAGAAACTTTGTATCTTTTAGGTGCAGAGCTCTTCGTCAT
TAATATCTTAGATTAGAGCA

Product: acetoin dehydrogenase, E1 component, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPI
VDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRRVVQAAEELAEEGISVEIVDPRT
LVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIK
DAIRKTYNKD

Sequences:

>Translated_330_residues
METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPI
VDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRRVVQAAEELAEEGISVEIVDPRT
LVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIK
DAIRKTYNKD
>Mature_330_residues
METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPI
VDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRRVVQAAEELAEEGISVEIVDPRT
LVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIK
DAIRKTYNKD

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=44.7852760736196, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=42.9447852760736, Blast_Score=264, Evalue=8e-71,
Organism=Homo sapiens, GI4557353, Length=331, Percent_Identity=38.6706948640483, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI34101272, Length=331, Percent_Identity=38.6706948640483, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI133778974, Length=281, Percent_Identity=24.9110320284698, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=327, Percent_Identity=43.4250764525994, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17506935, Length=331, Percent_Identity=39.2749244712991, Blast_Score=204, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=44.0366972477064, Blast_Score=285, Evalue=1e-77,
Organism=Drosophila melanogaster, GI21358145, Length=329, Percent_Identity=41.6413373860182, Blast_Score=270, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24650940, Length=329, Percent_Identity=41.6413373860182, Blast_Score=270, Evalue=7e-73,
Organism=Drosophila melanogaster, GI160714828, Length=331, Percent_Identity=37.7643504531722, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI160714832, Length=331, Percent_Identity=37.7643504531722, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24650943, Length=95, Percent_Identity=38.9473684210526, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24650945, Length=95, Percent_Identity=38.9473684210526, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24645119, Length=250, Percent_Identity=27.6, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551847, Length=250, Percent_Identity=27.6, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550715, Length=250, Percent_Identity=27.6, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35820; Mature: 35820

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPI
CCCCCCCHHHEEEEEECHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHCCCC
SEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEE
AGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLLKSSIRDNNPVIILEYKSEFN
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
QKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRRVVQAAEELAEEGISVEIVDPRT
CCCCCCCCCCCEEECCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDV
ECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PMPYAQNLENAMIPTVESIKDAIRKTYNKD
CCCHHHCCCHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSVGMLEEFGPERVRDCPI
CCCCCCCHHHEEEEEECHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHCCCC
SEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAKTRYMFGGKGQVPMTVRCA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEE
AGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLLKSSIRDNNPVIILEYKSEFN
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
QKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRRVVQAAEELAEEGISVEIVDPRT
CCCCCCCCCCCEEECCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEISAIISESEAFDYLDAPIRRCAGEDV
ECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PMPYAQNLENAMIPTVESIKDAIRKTYNKD
CCCHHHCCCHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]