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The map label for this gene is dnaB [H]

Identifier: 116515276

GI number: 116515276

Start: 382752

End: 384125

Strand: Direct

Name: dnaB [H]

Synonym: BCc_359

Alternate gene names: 116515276

Gene position: 382752-384125 (Clockwise)

Preceding gene: 116515275

Following gene: 116515277

Centisome position: 91.92

GC content: 26.27

Gene sequence:

>1374_bases
ATGATAGAATCAATAATAAATAAAAAAACAGAACTCAAAATACCTCCATATTCACTAGAAGCTGAACAATCAGTGTTAGG
AGGATTAATGTTAGATAATCAAAAATGGGATATAATTTCTGAATATATTGTTATGGAAGATTTTTATAATCGACAACATC
AGTTAATATTTTGTGAAATGAAATATCTAATTGAAAAAGGATCTCCCATAGATTTAATCACATTATCAGAACCATTAGAA
CAAAAAGGAGAATTAAATAATGTTGGGAGATTTTCTTATTTAGCTGAAATTTCAAAAAACACACCAAGTATTACAAATAT
ATTAGCATATGCAGAAATTATTAGAGAAAGAGCTATTATTAGAGAAATTATTTTAACAGCAAATAATATAGCAAATGCAG
GGTATTACCCAAAAGGAAGAAAAAGTACGGAATTATTAGACTATGCTGAATCTAGTATTTTTAAAATTTCAGAAATACGT
TCAAAAAATAATAATGGACCTAAAAATATAGAAACAATTCTTGATTCAACTATTCAATCTATTGAAAAACTTATTAAACA
GCCAAATAATGGAATTACGGGATTAAATACTGGATATCATGATTTAAATAAAAAAACATCTGGATTACAACAATCTGATT
TAATAATAATTGCTGCAAGACCATCCATGGGAAAAACTACATTTGCTATGAATTTATGTGAAAACACAGCTATGTTATAT
AAAAAACCAATTTTAATATTTAGTTTAGAAATGCCTGGAGAGCAAATTATGATTCGTATGTTGGCTTCATTATCACGTGT
TAATCAATCAAAAATTCGAACAGGAAAATTAAATAATGAAGAATGGAGTCGAATATCTAGTACTATGCAAATTTTATTAA
AAAAAAAAAATATTTATATTGATGATTCTTCAGGTCTTACACCTAATGAAGTTCGTTCACGTTCACGGAAAATATATCGT
GAAAATAATGGACTAAGTTTAATTATGATAGATTATCTTCAATTAATTAAAATACCATCATTATCAGGAAATAGAACCTT
AGAAATGCAGAATTTCAGAACATTAAAATCATTAGCAAAAGAATTAAATATTCCAATTATTGCTTTGTCTCAATTAAATA
GATCATTGGAACAAAGATCAGATAAAAGACCGGTAAATTCAGATTTAAGAGAATCTGGTTCATTAGAACAAGATGCAGAT
TTAATCCTATTTATTTATAGAGATGAATTATATCATGAAAATAGTGAATTAAAAGGAATTGCTGAAATTATTATTGGTAA
ACAAAGAAATGGTCCAACTGGAACAATACGATTAATATTCAATGGTCAATGGTCTAGATTTGATAATTACGCTCATACGA
AATATCATTGTTAA

Upstream 100 bases:

>100_bases
AAAAATTTTTATAAAATATCAATTTTAAATGCCAAAAAAATTATTTTATATAATAATTTTATATATATTTTAAAAATTTA
TATAAGAGAATCAATTATTT

Downstream 100 bases:

>100_bases
TAAAAATAAAAAAGCATTTATATTGAATAATAATATTAATATAAGTAAAATACTTATATAAAAATATAAAAAACTAATTA
TATAAACTATCAATTATTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIESIINKKTELKIPPYSLEAEQSVLGGLMLDNQKWDIISEYIVMEDFYNRQHQLIFCEMKYLIEKGSPIDLITLSEPLE
QKGELNNVGRFSYLAEISKNTPSITNILAYAEIIRERAIIREIILTANNIANAGYYPKGRKSTELLDYAESSIFKISEIR
SKNNNGPKNIETILDSTIQSIEKLIKQPNNGITGLNTGYHDLNKKTSGLQQSDLIIIAARPSMGKTTFAMNLCENTAMLY
KKPILIFSLEMPGEQIMIRMLASLSRVNQSKIRTGKLNNEEWSRISSTMQILLKKKNIYIDDSSGLTPNEVRSRSRKIYR
ENNGLSLIMIDYLQLIKIPSLSGNRTLEMQNFRTLKSLAKELNIPIIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDAD
LILFIYRDELYHENSELKGIAEIIIGKQRNGPTGTIRLIFNGQWSRFDNYAHTKYHC

Sequences:

>Translated_457_residues
MIESIINKKTELKIPPYSLEAEQSVLGGLMLDNQKWDIISEYIVMEDFYNRQHQLIFCEMKYLIEKGSPIDLITLSEPLE
QKGELNNVGRFSYLAEISKNTPSITNILAYAEIIRERAIIREIILTANNIANAGYYPKGRKSTELLDYAESSIFKISEIR
SKNNNGPKNIETILDSTIQSIEKLIKQPNNGITGLNTGYHDLNKKTSGLQQSDLIIIAARPSMGKTTFAMNLCENTAMLY
KKPILIFSLEMPGEQIMIRMLASLSRVNQSKIRTGKLNNEEWSRISSTMQILLKKKNIYIDDSSGLTPNEVRSRSRKIYR
ENNGLSLIMIDYLQLIKIPSLSGNRTLEMQNFRTLKSLAKELNIPIIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDAD
LILFIYRDELYHENSELKGIAEIIIGKQRNGPTGTIRLIFNGQWSRFDNYAHTKYHC
>Mature_457_residues
MIESIINKKTELKIPPYSLEAEQSVLGGLMLDNQKWDIISEYIVMEDFYNRQHQLIFCEMKYLIEKGSPIDLITLSEPLE
QKGELNNVGRFSYLAEISKNTPSITNILAYAEIIRERAIIREIILTANNIANAGYYPKGRKSTELLDYAESSIFKISEIR
SKNNNGPKNIETILDSTIQSIEKLIKQPNNGITGLNTGYHDLNKKTSGLQQSDLIIIAARPSMGKTTFAMNLCENTAMLY
KKPILIFSLEMPGEQIMIRMLASLSRVNQSKIRTGKLNNEEWSRISSTMQILLKKKNIYIDDSSGLTPNEVRSRSRKIYR
ENNGLSLIMIDYLQLIKIPSLSGNRTLEMQNFRTLKSLAKELNIPIIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDAD
LILFIYRDELYHENSELKGIAEIIIGKQRNGPTGTIRLIFNGQWSRFDNYAHTKYHC

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=445, Percent_Identity=70.7865168539326, Blast_Score=683, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52209; Mature: 52209

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESIINKKTELKIPPYSLEAEQSVLGGLMLDNQKWDIISEYIVMEDFYNRQHQLIFCEM
CCHHHHCCCCEECCCCCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEH
KYLIEKGSPIDLITLSEPLEQKGELNNVGRFSYLAEISKNTPSITNILAYAEIIRERAII
HHHHCCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
REIILTANNIANAGYYPKGRKSTELLDYAESSIFKISEIRSKNNNGPKNIETILDSTIQS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEKLIKQPNNGITGLNTGYHDLNKKTSGLQQSDLIIIAARPSMGKTTFAMNLCENTAMLY
HHHHHHCCCCCCCCCCCCHHHHCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHH
KKPILIFSLEMPGEQIMIRMLASLSRVNQSKIRTGKLNNEEWSRISSTMQILLKKKNIYI
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEE
DDSSGLTPNEVRSRSRKIYRENNGLSLIMIDYLQLIKIPSLSGNRTLEMQNFRTLKSLAK
ECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
ELNIPIIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDADLILFIYRDELYHENSELKGI
HCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHCCCCHHHHH
AEIIIGKQRNGPTGTIRLIFNGQWSRFDNYAHTKYHC
HHHHHCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MIESIINKKTELKIPPYSLEAEQSVLGGLMLDNQKWDIISEYIVMEDFYNRQHQLIFCEM
CCHHHHCCCCEECCCCCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEH
KYLIEKGSPIDLITLSEPLEQKGELNNVGRFSYLAEISKNTPSITNILAYAEIIRERAII
HHHHCCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
REIILTANNIANAGYYPKGRKSTELLDYAESSIFKISEIRSKNNNGPKNIETILDSTIQS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEKLIKQPNNGITGLNTGYHDLNKKTSGLQQSDLIIIAARPSMGKTTFAMNLCENTAMLY
HHHHHHCCCCCCCCCCCCHHHHCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHH
KKPILIFSLEMPGEQIMIRMLASLSRVNQSKIRTGKLNNEEWSRISSTMQILLKKKNIYI
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEE
DDSSGLTPNEVRSRSRKIYRENNGLSLIMIDYLQLIKIPSLSGNRTLEMQNFRTLKSLAK
ECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
ELNIPIIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDADLILFIYRDELYHENSELKGI
HCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHCCCCHHHHH
AEIIIGKQRNGPTGTIRLIFNGQWSRFDNYAHTKYHC
HHHHHCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]