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The map label for this gene is folD

Identifier: 116515226

GI number: 116515226

Start: 346377

End: 347231

Strand: Direct

Name: folD

Synonym: BCc_305

Alternate gene names: 116515226

Gene position: 346377-347231 (Clockwise)

Preceding gene: 116515225

Following gene: 116515228

Centisome position: 83.19

GC content: 23.16

Gene sequence:

>855_bases
ATGGAAACAAAAATTCTTGATGGATTAAAAATTTCAAAGAAAATTATAAAAAAAATAAAAAAAAAAATAGAAAAAAGAAA
AAAAAAAAGAAAAAAAATTCCTGGTTTAGCTATGATTGTAATTGGAAATAATCCAGCTTCATTAATATATGTTAATAAAA
AAAGAGAAGCATGTAATCAAGCAGGATTTTTTTCTATATATTGGCATTTATCAAATCAAATAGAAGAAATAGAATTAATT
AATTTAATTAAAAAATTAAACAAAAACAAATGTATTGATGGTATTTTAATTCAATTACCATTACCTATTAAAATAAATTA
TTTAAAAATAATTACCAGTATTGATCCGAAAAAAGATGTAGATGGTTTCCATCCTTATAATTTAGGATCTTTATGTCAAA
ATAATCCTCAATTTAGATCATGTACTTCTAAAGGAGTAATTACATTACTTAAAAAATATAAAATCAATATACATGGATTA
TATGCAGTAATTATAGGATCATCTAATATAGTTGGAAAACCTATGTATATGGAATTATTATTAGCGGGATGTACAGTAAC
AATTGTAAACAAAAATACAAAAAACATAAAAACATATGTTAAAAAAGCTGATTTAGTTGTGATTGCTATTGGTCAACCAA
ATTTTTTATATGGACATTGGATTAAACTAGGAGCTATTGTAATAGATATTGGAATTAATTATTTGTATAATAAAAACAAA
TATAAAATTGTTGGTGATGTACATTTTAAATCTACTTCAGTAAAAACGTCTTATATTACTCCAGTACCAGGAGGAGTAGG
TCCTATGACCGTAGTATCATTATTAGAAAATACACTACAAGCGTGTATAATGTAA

Upstream 100 bases:

>100_bases
GTGATTAAATAACAATAAATAAATTTTTACTAAAAATAAGATTAATAATAAAAAATATTTCTATAAAATAGAAAGAAAAA
AATTAGGTTATATAAAAAAT

Downstream 100 bases:

>100_bases
AAATATTTAATTATATAAATCTATAATATGAAGAATAAGAATTATCTTCTACAATAACACCTAAACAAAACAATTTTTTT
CTAATAATGTCTGATAAATT

Product: 5,10-methylene-tetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
METKILDGLKISKKIIKKIKKKIEKRKKKRKKIPGLAMIVIGNNPASLIYVNKKREACNQAGFFSIYWHLSNQIEEIELI
NLIKKLNKNKCIDGILIQLPLPIKINYLKIITSIDPKKDVDGFHPYNLGSLCQNNPQFRSCTSKGVITLLKKYKINIHGL
YAVIIGSSNIVGKPMYMELLLAGCTVTIVNKNTKNIKTYVKKADLVVIAIGQPNFLYGHWIKLGAIVIDIGINYLYNKNK
YKIVGDVHFKSTSVKTSYITPVPGGVGPMTVVSLLENTLQACIM

Sequences:

>Translated_284_residues
METKILDGLKISKKIIKKIKKKIEKRKKKRKKIPGLAMIVIGNNPASLIYVNKKREACNQAGFFSIYWHLSNQIEEIELI
NLIKKLNKNKCIDGILIQLPLPIKINYLKIITSIDPKKDVDGFHPYNLGSLCQNNPQFRSCTSKGVITLLKKYKINIHGL
YAVIIGSSNIVGKPMYMELLLAGCTVTIVNKNTKNIKTYVKKADLVVIAIGQPNFLYGHWIKLGAIVIDIGINYLYNKNK
YKIVGDVHFKSTSVKTSYITPVPGGVGPMTVVSLLENTLQACIM
>Mature_284_residues
METKILDGLKISKKIIKKIKKKIEKRKKKRKKIPGLAMIVIGNNPASLIYVNKKREACNQAGFFSIYWHLSNQIEEIELI
NLIKKLNKNKCIDGILIQLPLPIKINYLKIITSIDPKKDVDGFHPYNLGSLCQNNPQFRSCTSKGVITLLKKYKINIHGL
YAVIIGSSNIVGKPMYMELLLAGCTVTIVNKNTKNIKTYVKKADLVVIAIGQPNFLYGHWIKLGAIVIDIGINYLYNKNK
YKIVGDVHFKSTSVKTSYITPVPGGVGPMTVVSLLENTLQACIM

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=286, Percent_Identity=39.5104895104895, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=37.542662116041, Blast_Score=191, Evalue=4e-49,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=39.2491467576792, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI36796743, Length=231, Percent_Identity=24.2424242424242, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=53.356890459364, Blast_Score=334, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=37.1527777777778, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=284, Percent_Identity=42.9577464788732, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=40.2684563758389, Blast_Score=212, Evalue=6e-56,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=36.3013698630137, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=36.3013698630137, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=35.0877192982456, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=35.0877192982456, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24645718, Length=285, Percent_Identity=35.0877192982456, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17137370, Length=285, Percent_Identity=35.0877192982456, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BUCCC (Q057D7)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802855.1
- ProteinModelPortal:   Q057D7
- SMR:   Q057D7
- STRING:   Q057D7
- EnsemblBacteria:   EBBUCT00000001247
- GeneID:   4440912
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_305
- eggNOG:   COG0190
- GeneTree:   EBGT00050000007792
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- BioCyc:   BAPH372461:BCC_305-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31857; Mature: 31857

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKILDGLKISKKIIKKIKKKIEKRKKKRKKIPGLAMIVIGNNPASLIYVNKKREACNQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHCC
AGFFSIYWHLSNQIEEIELINLIKKLNKNKCIDGILIQLPLPIKINYLKIITSIDPKKDV
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEEECCCCCCCC
DGFHPYNLGSLCQNNPQFRSCTSKGVITLLKKYKINIHGLYAVIIGSSNIVGKPMYMELL
CCCCCCCCHHHHCCCCCHHHHCCCHHHHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHH
LAGCTVTIVNKNTKNIKTYVKKADLVVIAIGQPNFLYGHWIKLGAIVIDIGINYLYNKNK
HHCCEEEEEECCCHHHHHHHEECCEEEEEECCCCCEEHHHHHHCEEEEEECCEEEECCCC
YKIVGDVHFKSTSVKTSYITPVPGGVGPMTVVSLLENTLQACIM
EEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
METKILDGLKISKKIIKKIKKKIEKRKKKRKKIPGLAMIVIGNNPASLIYVNKKREACNQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHCC
AGFFSIYWHLSNQIEEIELINLIKKLNKNKCIDGILIQLPLPIKINYLKIITSIDPKKDV
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEEECCCCCCCC
DGFHPYNLGSLCQNNPQFRSCTSKGVITLLKKYKINIHGLYAVIIGSSNIVGKPMYMELL
CCCCCCCCHHHHCCCCCHHHHCCCHHHHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHH
LAGCTVTIVNKNTKNIKTYVKKADLVVIAIGQPNFLYGHWIKLGAIVIDIGINYLYNKNK
HHCCEEEEEECCCHHHHHHHEECCEEEEEECCCCCEEHHHHHHCEEEEEECCEEEECCCC
YKIVGDVHFKSTSVKTSYITPVPGGVGPMTVVSLLENTLQACIM
EEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA