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The map label for this gene is miaB

Identifier: 116515198

GI number: 116515198

Start: 305849

End: 307156

Strand: Direct

Name: miaB

Synonym: BCc_272

Alternate gene names: 116515198

Gene position: 305849-307156 (Clockwise)

Preceding gene: 116515197

Following gene: 116515199

Centisome position: 73.45

GC content: 20.72

Gene sequence:

>1308_bases
ATGAATGAACATGATTCCTCAATCATAGAAAATATATTAAAAAAAACAAATTTATATATAATTACAAAAAAACCAGAAAT
ATCTGATATTTTAATTTTAAATACATGCTCTATTAGAGAAAAAGCTCAAGAAAAATTATTCCATCAATTAGGACGTTGGA
AAAAACTAAAACAAAAAAATTCAAAAATATTAATTGCTGTAGGAGGATGTGTTGCTGTTCAAGAAGGAAAGAAAATTTAT
AAAAGAGCAAAATTTATTGATATAATTTTTGGACCACAAACATTACATAAATTACCTAAGTTATTAATAGAATCAAATAA
AAAAAAAAGTCTTATTATAAATATTAAAAAAAAATCATTAAAAAAATTTAATTATACAATAAATAAAAATACAAATATAA
AAAAAAAATTTTCTTCATTTGTAACTATTATGGAAGGATGTAATAAATATTGTTCTTTTTGTATTGTTCCATATACAAGA
GGAAAAGAAGTTAGTAGAAATAATAAAAAAATAATTTCAGAAATAATAGAATTATCTAAAAAAGGAGTACGTGAAATAAC
ACTTTTAGGACAAAATGTAAATGCATATAAATTTTCTGATACTTTTAATAAAAAAAATTATAGTTTTTCAGATTTATTAT
ACTCAATATCAGAAATCCCTAGAATTGATAGAATTCGATTTATTACCAGTCATCCTGTGGAATTTAATAACAATATAATA
GAAGCATATAAAAAAATTCCTAAATTAACAAATTTTCTACACTTACCGGTACAAAGTGGTTCTAATAAAATTTTAAAATT
AATGAAACGAGGATATACTATAGAAAAATATGAAAACATTGTAAATAAAATAAAAAAAATACGTCCAAAAATAAATATTA
GTTCAGATTTTATTATCGGATTTCCAGGTGAAACTAAAGAAGATTTTCAAAAAACAATTTATTTTATTTCAAAAATTAAT
TTCGATACAAGTTATAGTTTTATTTACTCAAAAAGACCTCGAACAAGAGCTTCTAAACTAGAAGATAATGTAACGATGGA
AGAAAAAAAAAAGAGATTATATAAAGTTCAACAAAAAATCAATCAACAAGCATTTCAATGGAAAAGAAGAAGTACTGAAC
AAATAGTTTTAGTAGAAGGAATTTCAAAAAACAATATACAAGAATTATATGGAAGAACAGAAAATAATAGAACAGTTTTT
TTTGAAGGTAATCCTAAATTTATTGGAAACTTTATAAAATTAAAAATTATAAGTATTAAATATAATACTTTTCTAAAAGG
AAAAATTATTTCTAATAATTATTTTTAG

Upstream 100 bases:

>100_bases
ACTAAAAAAAATATAAATAAATATTATTTAAAAGTTAAAAATTATTTATGAAAAAAAACAAAAAAAAAAAAAAATATATA
TTTAAACTTGGGGATGTCAA

Downstream 100 bases:

>100_bases
TAATAGAATAATTAAAAATTTTGTAAAAAATTGATGAATATAAAGAAAAACTTAATGAAAATTGATATTCAAAATGTATG
TAAGAAAAATATTTTTTTTC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKNSKILIAVGGCVAVQEGKKIY
KRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSLKKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTR
GKEVSRNNKKIISEIIELSKKGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII
EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIGFPGETKEDFQKTIYFISKIN
FDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKINQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVF
FEGNPKFIGNFIKLKIISIKYNTFLKGKIISNNYF

Sequences:

>Translated_435_residues
MNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKNSKILIAVGGCVAVQEGKKIY
KRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSLKKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTR
GKEVSRNNKKIISEIIELSKKGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII
EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIGFPGETKEDFQKTIYFISKIN
FDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKINQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVF
FEGNPKFIGNFIKLKIISIKYNTFLKGKIISNNYF
>Mature_435_residues
MNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKNSKILIAVGGCVAVQEGKKIY
KRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSLKKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTR
GKEVSRNNKKIISEIIELSKKGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII
EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIGFPGETKEDFQKTIYFISKIN
FDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKINQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVF
FEGNPKFIGNFIKLKIISIKYNTFLKGKIISNNYF

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=471, Percent_Identity=33.5456475583864, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI28872784, Length=471, Percent_Identity=33.5456475583864, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI93277076, Length=427, Percent_Identity=26.2295081967213, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1786882, Length=432, Percent_Identity=53.2407407407407, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1787057, Length=416, Percent_Identity=25, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=30.9677419354839, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=222, Percent_Identity=27.9279279279279, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356207, Length=423, Percent_Identity=33.5697399527187, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI19922432, Length=399, Percent_Identity=24.0601503759398, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BUCCC (Q057G5)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802827.1
- ProteinModelPortal:   Q057G5
- SMR:   Q057G5
- STRING:   Q057G5
- EnsemblBacteria:   EBBUCT00000001449
- GeneID:   4440858
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_272
- eggNOG:   COG0621
- GeneTree:   EBGT00050000007978
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- BioCyc:   BAPH372461:BCC_272-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50961; Mature: 50961

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKN
CCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
SKILIAVGGCVAVQEGKKIYKRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSL
CEEEEEECCEEEEHHHHHHHHHHHEEEEEECHHHHHHHHHHHHCCCCCCEEEEEEEHHHH
KKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTRGKEVSRNNKKIISEIIELSK
HHHCEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHH
KGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII
CCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIG
HHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEE
FPGETKEDFQKTIYFISKINFDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKI
CCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
NQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVFFEGNPKFIGNFIKLKIISIK
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCHHHHCCEEEEEEEEEE
YNTFLKGKIISNNYF
ECCEEEEEEECCCCC
>Mature Secondary Structure
MNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKN
CCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
SKILIAVGGCVAVQEGKKIYKRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSL
CEEEEEECCEEEEHHHHHHHHHHHEEEEEECHHHHHHHHHHHHCCCCCCEEEEEEEHHHH
KKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTRGKEVSRNNKKIISEIIELSK
HHHCEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHH
KGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII
CCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIG
HHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEE
FPGETKEDFQKTIYFISKINFDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKI
CCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
NQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVFFEGNPKFIGNFIKLKIISIK
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCHHHHCCEEEEEEEEEE
YNTFLKGKIISNNYF
ECCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA