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The map label for this gene is eno

Identifier: 116515190

GI number: 116515190

Start: 293837

End: 295135

Strand: Direct

Name: eno

Synonym: BCc_264

Alternate gene names: 116515190

Gene position: 293837-295135 (Clockwise)

Preceding gene: 116515189

Following gene: 116515191

Centisome position: 70.57

GC content: 24.63

Gene sequence:

>1299_bases
ATGTCAAAAATAAAAAAAATACTTTCTAGAGAAATTTTAGATTCACGTGGATACCCAACTATTGAAACAGAAGTACATTT
AAAAACTGGATATTTTGGATATTCTTCAGTTCCATCAGGAGCATCAACAGGATCAAAAGAAGCATTAGAACTTAGAGATA
ATGATAAAAATCGTTTTTTTGGTAAAGGAGTTAAAAAAGCGGTAAATTATGTTAATACCGAAATTTTTCAAGCATTAAAA
AATAAAAATGCAGAAGAACAAAAAGAATTAGATAATCTAATGATAAAACTAGATGGCACAAAAAATAAATCAAGATTTGG
AGCTAATGCAATATTATCTGTATCTTTATCTTTAGCTAAAGCAATAGCTTTAGAAAAAAATATACCTTTTTATTCTTATA
TTTCTGAAATTAATTATTCAAAAAATAATTTTTCTATTCCATTACCTATGATTAATATTATCAATGGTGGAATGCATGCA
AATAATAATATTGATCTTCAAGAATTTATGATACAACCTATAGGAGCAAAAAGTATCTTACAAGCTGTCCAAATGGGATC
AGAAATTTTTCATATGTTAGGAATTATTTTAAAAGAAAAAGGATATTCTACATGTGTAGGAGATGAAGGAGGATATGCTC
CTAATCTTAAATCTAATGAAGAAGCATTATCTATGATTAGTTTAGCTGTTGAACGTTCTAATTATAAATTAAATGAAGAT
ATAACATTTGCAATAGATTGCGCGTCTTCTGAATTATTTAATAAAAAAACTAGAAGATATAGACTAAAAAATGAAAATAA
AGAATATAATTCAATAGAATTTACTCATTATTTAGAAAAATTAACTAAAAAATATCCAATTCGATCTATAGAAGATGGAC
AAGATGAAAGTGATTGGAAAGGATTTCAATATCAAACAAAAATATTAGGAAAAAAGATACAATTAGTAGGTGATGACTTA
TTTGTTACTAATAAAAAATTTTTAGAATATGGAATTAAAAAAAAAGCAGCAAATTCTATTTTGATTAAATTAAATCAGAT
TGGAACATTAACTGAAACATTAGAAACTATAAAAAAAGCTCAAGAAAACAAATATAATGTAATTATATCACATAGATCTG
GAGAAACAGAAGATACTAGTATTGCTGATCTTGCAGTAGGTACAAATGCTGGACAAATAAAAACTGGTTCAATGTGCAGA
TCAGATCGTACATCTAAATATAATAGATTAATAAAAATAGAAGAATCTTTAAATACACAAAAAAAAACATTTAAAAAAAA
ATTAAATTTATTTAATTAG

Upstream 100 bases:

>100_bases
AAGATATAAAAATAATTAAGACAATTTATTTTAAAAAAAAAATTTTATTAAATAAAATTAAATTTTCATTAAAAAATTAA
TATATTTTAAAGGCTATAAT

Downstream 100 bases:

>100_bases
TTTAAAGATAATTTAAATTAATTTTATTTATAAAATATTATATTTATTAAATAGAGAAGCATTCTTTATTTAATAAATAT
ATAAAATTAAAAATATAGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSKIKKILSREILDSRGYPTIETEVHLKTGYFGYSSVPSGASTGSKEALELRDNDKNRFFGKGVKKAVNYVNTEIFQALK
NKNAEEQKELDNLMIKLDGTKNKSRFGANAILSVSLSLAKAIALEKNIPFYSYISEINYSKNNFSIPLPMINIINGGMHA
NNNIDLQEFMIQPIGAKSILQAVQMGSEIFHMLGIILKEKGYSTCVGDEGGYAPNLKSNEEALSMISLAVERSNYKLNED
ITFAIDCASSELFNKKTRRYRLKNENKEYNSIEFTHYLEKLTKKYPIRSIEDGQDESDWKGFQYQTKILGKKIQLVGDDL
FVTNKKFLEYGIKKKAANSILIKLNQIGTLTETLETIKKAQENKYNVIISHRSGETEDTSIADLAVGTNAGQIKTGSMCR
SDRTSKYNRLIKIEESLNTQKKTFKKKLNLFN

Sequences:

>Translated_432_residues
MSKIKKILSREILDSRGYPTIETEVHLKTGYFGYSSVPSGASTGSKEALELRDNDKNRFFGKGVKKAVNYVNTEIFQALK
NKNAEEQKELDNLMIKLDGTKNKSRFGANAILSVSLSLAKAIALEKNIPFYSYISEINYSKNNFSIPLPMINIINGGMHA
NNNIDLQEFMIQPIGAKSILQAVQMGSEIFHMLGIILKEKGYSTCVGDEGGYAPNLKSNEEALSMISLAVERSNYKLNED
ITFAIDCASSELFNKKTRRYRLKNENKEYNSIEFTHYLEKLTKKYPIRSIEDGQDESDWKGFQYQTKILGKKIQLVGDDL
FVTNKKFLEYGIKKKAANSILIKLNQIGTLTETLETIKKAQENKYNVIISHRSGETEDTSIADLAVGTNAGQIKTGSMCR
SDRTSKYNRLIKIEESLNTQKKTFKKKLNLFN
>Mature_431_residues
SKIKKILSREILDSRGYPTIETEVHLKTGYFGYSSVPSGASTGSKEALELRDNDKNRFFGKGVKKAVNYVNTEIFQALKN
KNAEEQKELDNLMIKLDGTKNKSRFGANAILSVSLSLAKAIALEKNIPFYSYISEINYSKNNFSIPLPMINIINGGMHAN
NNIDLQEFMIQPIGAKSILQAVQMGSEIFHMLGIILKEKGYSTCVGDEGGYAPNLKSNEEALSMISLAVERSNYKLNEDI
TFAIDCASSELFNKKTRRYRLKNENKEYNSIEFTHYLEKLTKKYPIRSIEDGQDESDWKGFQYQTKILGKKIQLVGDDLF
VTNKKFLEYGIKKKAANSILIKLNQIGTLTETLETIKKAQENKYNVIISHRSGETEDTSIADLAVGTNAGQIKTGSMCRS
DRTSKYNRLIKIEESLNTQKKTFKKKLNLFN

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=427, Percent_Identity=47.0725995316159, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4503571, Length=427, Percent_Identity=47.0725995316159, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI301897477, Length=422, Percent_Identity=46.4454976303318, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI301897469, Length=422, Percent_Identity=46.4454976303318, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI301897479, Length=421, Percent_Identity=41.8052256532066, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=26.2536873156342, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=26.2536873156342, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=26.2536873156342, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1789141, Length=419, Percent_Identity=64.4391408114558, Blast_Score=556, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71995829, Length=428, Percent_Identity=46.7289719626168, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17536383, Length=428, Percent_Identity=46.7289719626168, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32563855, Length=186, Percent_Identity=46.2365591397849, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6321693, Length=423, Percent_Identity=45.3900709219858, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=45.8628841607565, Blast_Score=355, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6324974, Length=423, Percent_Identity=45.8628841607565, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6324969, Length=423, Percent_Identity=45.8628841607565, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6321968, Length=426, Percent_Identity=45.0704225352113, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=45.7471264367816, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=45.7471264367816, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=45.7471264367816, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=45.7471264367816, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=45.7471264367816, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=45.7471264367816, Blast_Score=355, Evalue=2e-98,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BUCCC (Q057H3)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802819.1
- ProteinModelPortal:   Q057H3
- SMR:   Q057H3
- STRING:   Q057H3
- EnsemblBacteria:   EBBUCT00000001510
- GeneID:   4440850
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_264
- eggNOG:   COG0148
- GeneTree:   EBGT00050000007974
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   BAPH372461:BCC_264-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 48774; Mature: 48643

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKKILSREILDSRGYPTIETEVHLKTGYFGYSSVPSGASTGSKEALELRDNDKNRFF
CHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVKKAVNYVNTEIFQALKNKNAEEQKELDNLMIKLDGTKNKSRFGANAILSVSLSLAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
AIALEKNIPFYSYISEINYSKNNFSIPLPMINIINGGMHANNNIDLQEFMIQPIGAKSIL
HHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
QAVQMGSEIFHMLGIILKEKGYSTCVGDEGGYAPNLKSNEEALSMISLAVERSNYKLNED
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
ITFAIDCASSELFNKKTRRYRLKNENKEYNSIEFTHYLEKLTKKYPIRSIEDGQDESDWK
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFQYQTKILGKKIQLVGDDLFVTNKKFLEYGIKKKAANSILIKLNQIGTLTETLETIKKA
CCCHHHHHHCCEEEEECCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QENKYNVIISHRSGETEDTSIADLAVGTNAGQIKTGSMCRSDRTSKYNRLIKIEESLNTQ
HCCCEEEEEEECCCCCCCCCHHHEEECCCCCCEECCCHHHCCCHHHHHHEEEEHHHCCHH
KKTFKKKLNLFN
HHHHHHHHCCCC
>Mature Secondary Structure 
SKIKKILSREILDSRGYPTIETEVHLKTGYFGYSSVPSGASTGSKEALELRDNDKNRFF
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVKKAVNYVNTEIFQALKNKNAEEQKELDNLMIKLDGTKNKSRFGANAILSVSLSLAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
AIALEKNIPFYSYISEINYSKNNFSIPLPMINIINGGMHANNNIDLQEFMIQPIGAKSIL
HHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
QAVQMGSEIFHMLGIILKEKGYSTCVGDEGGYAPNLKSNEEALSMISLAVERSNYKLNED
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
ITFAIDCASSELFNKKTRRYRLKNENKEYNSIEFTHYLEKLTKKYPIRSIEDGQDESDWK
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFQYQTKILGKKIQLVGDDLFVTNKKFLEYGIKKKAANSILIKLNQIGTLTETLETIKKA
CCCHHHHHHCCEEEEECCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QENKYNVIISHRSGETEDTSIADLAVGTNAGQIKTGSMCRSDRTSKYNRLIKIEESLNTQ
HCCCEEEEEEECCCCCCCCCHHHEEECCCCCCEECCCHHHCCCHHHHHHEEEEHHHCCHH
KKTFKKKLNLFN
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA