| Definition | Buchnera aphidicola str. Cc (Cinara cedri), complete genome. |
|---|---|
| Accession | NC_008513 |
| Length | 416,380 |
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The map label for this gene is polA [H]
Identifier: 116515191
GI number: 116515191
Start: 295241
End: 297901
Strand: Direct
Name: polA [H]
Synonym: BCc_265
Alternate gene names: 116515191
Gene position: 295241-297901 (Clockwise)
Preceding gene: 116515190
Following gene: 116515193
Centisome position: 70.91
GC content: 14.88
Gene sequence:
>2661_bases ATGAAAAAAAAAATTATTATAATTGATGGTCATTATTGTTTATATCAAAATTATTTTTCATTTATAAAATTGAAAAATAA AAATGGATCTTCCACAGGAGTTTTGTATGGATATATAAGACTTCTTAATAAATTAATTGAAAAATTTAATCCAAAAAAAA TTATTATTGTGTTTGATACACCAAAAAAAACAAATCGACATAAATTATTTACTAAATATAAAAAAAATAGAATTTCTATG CCGAATGAACTTAAAAAACAAATTAATCCATTAAAAAAAATTATTAAAGCTTTAAATATAACAATTATAAGTATAAAAAA AATAGAAGCAGATGATATTATTGGAACAATATCAAGAATTTTTACAAAAAAAAAATATTATATTTTTATATATAGTGCAG ATAAAGATATGACTCAATTAATTAAAAAAAATGTTTTTGTTATACCAGGTAGTATAAAAAAAGTATTAAATAAAAATGAT ATTTTTAAAAAATATGGTGTATATCCCAAATCTATAGCAGATTTTTTATGCTTAGTTGGAGATTTTTCTGATAATATTCG TGGAGTATTAGGAATAGGAAAAAAAACAGCAAAAATTCTTTTACAATCTTTTTCATCTATAAAAAAAATTTATAAAAATA TAGAAAAAATTTCATTTTTACCTATTAAAAATATTAAAAATATTAAAAAAAATTTAGAAAAAAACAAAAAAAATACATTT TTATCATATCAATTAACTAAAATTAATAAAAATATAGAATTACCAAAAATACATTCTATAATAAAAAAAAATACATTAAA TATTGAATTTATAAAAAAAAAATTTGAATTTTATCAATTAAATGAATATTTAAAAAAAATAAATGATAATACATTTTCAA TTTTAAATATTTATAATAAAAAAAAAAAAATGAAAAAAAAACATAAATATATAGAAATCGTAAAAAAAAAAATATTATTA AAATTAATTAATAAAATAATTAAAAAAAAAATATTTGCAATATCTATATATGAAAAAAAAAAAAATGAAAAAAAAAAAAA ATTTTATTTATCTATAACAATAGAAAAACATGAAACATGGTGGTTTATTTATAAAAAAAAAAAAAATATTTCTATTAAAA AAATATTAAAATATTTAAGACCAATATTAGAAAACAATAAATATTATAAAATTGGAAAAAATTTAAAAAATGTTTTTCAT ATTTTTCAAGAATATAATATTCTATTTAGAGGAATACATTTTGATACTACAATCATAAATTATTATTATAAATTGAATTA TAAAAAAAACAAAAAATATCAAAATTTAATATATAAATATAAAAAAAATAAAAAAGAAAAGAGTTTTAAAAGAAAATTTA TAATAATGCAAGAATCTTTGATCTCATTAAAAATATATTTTTTATTTAAAAAAAATATAAAGAAAAAAAAGAGGGAAAAT TTTCAACTAATCGATATGTCCTTATTACCAATATTAGCTGAAATAGAAAATAATGGAGTTTTAATAAAAAAAAAAATATT AAAAAAACAAAAAAAAAATCATGAAAAAACTTTAAAAAAATTAAAAAAAAAAATATTCAAAATAACAAAAGAAAAATTTA ATATTAATTCATCAAAACAATTACAAAATATTTTATTTAAAAAATTTAATCTTCCTAAAATATATAAAACAAAATTAGGT AATATTTCTACCAATGAAATAGTATTAAAAAAATTATCAAAAATTCATATTTTACCAAAAATTATTTTACAATTTAGATT AATAAAAAAAATCATAAATACTTACTTAAGAAACTTAATAAAATCAATAAATAATAAAACTAATAGAATTCATACTACAT ATAATCAAATAAATACTTCAACAGGAAGATTATCTTCAAAAAATCCTAATTTACAAAATATTCCAATTAAAACAAAAATA GGAAGAAAATTAAGAATTGCATTCATAACAAAAAAAAAATGGTTATTATTAACAGCAGATTATTCTCACATTGAATTACG AATTATAGCTCATTATTCAAAAGATAAAAATTTAATAAAAGATTTATTAAAAAATAAAGATATCCATATTAGTACTGCAT CTCATATTTTTAATAAACCATTAGAAAAAATAAAAAAATATCATAGAAATATTGCAAAAACTATTAATTTTTCTATTTTA TATGGCATTAGTCCTTTCGGTCTTTCTAAAAAATTAAATATTTCTATAAAAAAATCAAAAAATTTAATTTATAGATATTT CTTAAAATATAACAAAATTAAAAGATTTATTAAAAACACATATAAAACAGCAAAAAAAAGGGGATATATAAAAACATTGT TCAAAAGAAAATTATATATTCCTAATATAAATTCAAAAAATATTTATTTAAAAAATAGTGCAAAAAGATTTTGTATAAAT CTTATGATACAGAATACAGCTTCAGATATTATAAAAAAATCTATGATTACATTACATCACTTATTTAAAAAAAAATTTCC TAAAGATATTAAAATAATTATGCAAATTCATGATGAATTAATATTTGAAATCAAAGAGAAAAAAAAAGACAAAATAATTG ATGTAATTAAAAAAATTATGGAAAATAATACTAAATTAATTATTCCATTATACATTACAACAAAAATAGGTAAAAATTGG AAAGAAATAAAAAGTATATAA
Upstream 100 bases:
>100_bases AGATAATTTAAATTAATTTTATTTATAAAATATTATATTTATTAAATAGAGAAGCATTCTTTATTTAATAAATATATAAA ATTAAAAATATAGAAATAAA
Downstream 100 bases:
>100_bases TTTATTATATTTTACTTAAAAAATACCATTTAGATAATATTTTTCTAATAAATTGAATATTATTTTTATAAAATTTTGAA AAACTACATATGGTTATATT
Product: 5'-3' exonuclease domain-containing protein
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 886; Mature: 886
Protein sequence:
>886_residues MKKKIIIIDGHYCLYQNYFSFIKLKNKNGSSTGVLYGYIRLLNKLIEKFNPKKIIIVFDTPKKTNRHKLFTKYKKNRISM PNELKKQINPLKKIIKALNITIISIKKIEADDIIGTISRIFTKKKYYIFIYSADKDMTQLIKKNVFVIPGSIKKVLNKND IFKKYGVYPKSIADFLCLVGDFSDNIRGVLGIGKKTAKILLQSFSSIKKIYKNIEKISFLPIKNIKNIKKNLEKNKKNTF LSYQLTKINKNIELPKIHSIIKKNTLNIEFIKKKFEFYQLNEYLKKINDNTFSILNIYNKKKKMKKKHKYIEIVKKKILL KLINKIIKKKIFAISIYEKKKNEKKKKFYLSITIEKHETWWFIYKKKKNISIKKILKYLRPILENNKYYKIGKNLKNVFH IFQEYNILFRGIHFDTTIINYYYKLNYKKNKKYQNLIYKYKKNKKEKSFKRKFIIMQESLISLKIYFLFKKNIKKKKREN FQLIDMSLLPILAEIENNGVLIKKKILKKQKKNHEKTLKKLKKKIFKITKEKFNINSSKQLQNILFKKFNLPKIYKTKLG NISTNEIVLKKLSKIHILPKIILQFRLIKKIINTYLRNLIKSINNKTNRIHTTYNQINTSTGRLSSKNPNLQNIPIKTKI GRKLRIAFITKKKWLLLTADYSHIELRIIAHYSKDKNLIKDLLKNKDIHISTASHIFNKPLEKIKKYHRNIAKTINFSIL YGISPFGLSKKLNISIKKSKNLIYRYFLKYNKIKRFIKNTYKTAKKRGYIKTLFKRKLYIPNINSKNIYLKNSAKRFCIN LMIQNTASDIIKKSMITLHHLFKKKFPKDIKIIMQIHDELIFEIKEKKKDKIIDVIKKIMENNTKLIIPLYITTKIGKNW KEIKSI
Sequences:
>Translated_886_residues MKKKIIIIDGHYCLYQNYFSFIKLKNKNGSSTGVLYGYIRLLNKLIEKFNPKKIIIVFDTPKKTNRHKLFTKYKKNRISM PNELKKQINPLKKIIKALNITIISIKKIEADDIIGTISRIFTKKKYYIFIYSADKDMTQLIKKNVFVIPGSIKKVLNKND IFKKYGVYPKSIADFLCLVGDFSDNIRGVLGIGKKTAKILLQSFSSIKKIYKNIEKISFLPIKNIKNIKKNLEKNKKNTF LSYQLTKINKNIELPKIHSIIKKNTLNIEFIKKKFEFYQLNEYLKKINDNTFSILNIYNKKKKMKKKHKYIEIVKKKILL KLINKIIKKKIFAISIYEKKKNEKKKKFYLSITIEKHETWWFIYKKKKNISIKKILKYLRPILENNKYYKIGKNLKNVFH IFQEYNILFRGIHFDTTIINYYYKLNYKKNKKYQNLIYKYKKNKKEKSFKRKFIIMQESLISLKIYFLFKKNIKKKKREN FQLIDMSLLPILAEIENNGVLIKKKILKKQKKNHEKTLKKLKKKIFKITKEKFNINSSKQLQNILFKKFNLPKIYKTKLG NISTNEIVLKKLSKIHILPKIILQFRLIKKIINTYLRNLIKSINNKTNRIHTTYNQINTSTGRLSSKNPNLQNIPIKTKI GRKLRIAFITKKKWLLLTADYSHIELRIIAHYSKDKNLIKDLLKNKDIHISTASHIFNKPLEKIKKYHRNIAKTINFSIL YGISPFGLSKKLNISIKKSKNLIYRYFLKYNKIKRFIKNTYKTAKKRGYIKTLFKRKLYIPNINSKNIYLKNSAKRFCIN LMIQNTASDIIKKSMITLHHLFKKKFPKDIKIIMQIHDELIFEIKEKKKDKIIDVIKKIMENNTKLIIPLYITTKIGKNW KEIKSI >Mature_886_residues MKKKIIIIDGHYCLYQNYFSFIKLKNKNGSSTGVLYGYIRLLNKLIEKFNPKKIIIVFDTPKKTNRHKLFTKYKKNRISM PNELKKQINPLKKIIKALNITIISIKKIEADDIIGTISRIFTKKKYYIFIYSADKDMTQLIKKNVFVIPGSIKKVLNKND IFKKYGVYPKSIADFLCLVGDFSDNIRGVLGIGKKTAKILLQSFSSIKKIYKNIEKISFLPIKNIKNIKKNLEKNKKNTF LSYQLTKINKNIELPKIHSIIKKNTLNIEFIKKKFEFYQLNEYLKKINDNTFSILNIYNKKKKMKKKHKYIEIVKKKILL KLINKIIKKKIFAISIYEKKKNEKKKKFYLSITIEKHETWWFIYKKKKNISIKKILKYLRPILENNKYYKIGKNLKNVFH IFQEYNILFRGIHFDTTIINYYYKLNYKKNKKYQNLIYKYKKNKKEKSFKRKFIIMQESLISLKIYFLFKKNIKKKKREN FQLIDMSLLPILAEIENNGVLIKKKILKKQKKNHEKTLKKLKKKIFKITKEKFNINSSKQLQNILFKKFNLPKIYKTKLG NISTNEIVLKKLSKIHILPKIILQFRLIKKIINTYLRNLIKSINNKTNRIHTTYNQINTSTGRLSSKNPNLQNIPIKTKI GRKLRIAFITKKKWLLLTADYSHIELRIIAHYSKDKNLIKDLLKNKDIHISTASHIFNKPLEKIKKYHRNIAKTINFSIL YGISPFGLSKKLNISIKKSKNLIYRYFLKYNKIKRFIKNTYKTAKKRGYIKTLFKRKLYIPNINSKNIYLKNSAKRFCIN LMIQNTASDIIKKSMITLHHLFKKKFPKDIKIIMQIHDELIFEIKEKKKDKIIDVIKKIMENNTKLIIPLYITTKIGKNW KEIKSI
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=436, Percent_Identity=28.4403669724771, Blast_Score=215, Evalue=2e-55, Organism=Homo sapiens, GI139394648, Length=515, Percent_Identity=25.631067961165, Blast_Score=170, Evalue=6e-42, Organism=Escherichia coli, GI1790294, Length=926, Percent_Identity=34.9892008639309, Blast_Score=602, Evalue=1e-173, Organism=Escherichia coli, GI226510970, Length=215, Percent_Identity=28.3720930232558, Blast_Score=96, Evalue=1e-20, Organism=Caenorhabditis elegans, GI86563272, Length=378, Percent_Identity=28.3068783068783, Blast_Score=157, Evalue=2e-38, Organism=Drosophila melanogaster, GI17933644, Length=451, Percent_Identity=28.6031042128603, Blast_Score=196, Evalue=8e-50,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 105893; Mature: 105893
Theoretical pI: Translated: 10.99; Mature: 10.99
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKKIIIIDGHYCLYQNYFSFIKLKNKNGSSTGVLYGYIRLLNKLIEKFNPKKIIIVFDT CCCEEEEEECCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC PKKTNRHKLFTKYKKNRISMPNELKKQINPLKKIIKALNITIISIKKIEADDIIGTISRI CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECHHHHHHHHHHH FTKKKYYIFIYSADKDMTQLIKKNVFVIPGSIKKVLNKNDIFKKYGVYPKSIADFLCLVG HCCCEEEEEEEECCHHHHHHHHCCEEEECCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHC DFSDNIRGVLGIGKKTAKILLQSFSSIKKIYKNIEKISFLPIKNIKNIKKNLEKNKKNTF CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCE LSYQLTKINKNIELPKIHSIIKKNTLNIEFIKKKFEFYQLNEYLKKINDNTFSILNIYNK EEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH KKKMKKKHKYIEIVKKKILLKLINKIIKKKIFAISIYEKKKNEKKKKFYLSITIEKHETW HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCEEEEEEEEEEECCEE WFIYKKKKNISIKKILKYLRPILENNKYYKIGKNLKNVFHIFQEYNILFRGIHFDTTIIN EEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHEEEEEHHHEEEE YYYKLNYKKNKKYQNLIYKYKKNKKEKSFKRKFIIMQESLISLKIYFLFKKNIKKKKREN EEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHCC FQLIDMSLLPILAEIENNGVLIKKKILKKQKKNHEKTLKKLKKKIFKITKEKFNINSSKQ CEEEEHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH LQNILFKKFNLPKIYKTKLGNISTNEIVLKKLSKIHILPKIILQFRLIKKIINTYLRNLI HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KSINNKTNRIHTTYNQINTSTGRLSSKNPNLQNIPIKTKIGRKLRIAFITKKKWLLLTAD HHHCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEEECCCEEEEEEC YSHIELRIIAHYSKDKNLIKDLLKNKDIHISTASHIFNKPLEKIKKYHRNIAKTINFSIL CCEEEEEEEEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEE YGISPFGLSKKLNISIKKSKNLIYRYFLKYNKIKRFIKNTYKTAKKRGYIKTLFKRKLYI EECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC PNINSKNIYLKNSAKRFCINLMIQNTASDIIKKSMITLHHLFKKKFPKDIKIIMQIHDEL CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH IFEIKEKKKDKIIDVIKKIMENNTKLIIPLYITTKIGKNWKEIKSI HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHCC >Mature Secondary Structure MKKKIIIIDGHYCLYQNYFSFIKLKNKNGSSTGVLYGYIRLLNKLIEKFNPKKIIIVFDT CCCEEEEEECCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC PKKTNRHKLFTKYKKNRISMPNELKKQINPLKKIIKALNITIISIKKIEADDIIGTISRI CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECHHHHHHHHHHH FTKKKYYIFIYSADKDMTQLIKKNVFVIPGSIKKVLNKNDIFKKYGVYPKSIADFLCLVG HCCCEEEEEEEECCHHHHHHHHCCEEEECCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHC DFSDNIRGVLGIGKKTAKILLQSFSSIKKIYKNIEKISFLPIKNIKNIKKNLEKNKKNTF CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCE LSYQLTKINKNIELPKIHSIIKKNTLNIEFIKKKFEFYQLNEYLKKINDNTFSILNIYNK EEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH KKKMKKKHKYIEIVKKKILLKLINKIIKKKIFAISIYEKKKNEKKKKFYLSITIEKHETW HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCEEEEEEEEEEECCEE WFIYKKKKNISIKKILKYLRPILENNKYYKIGKNLKNVFHIFQEYNILFRGIHFDTTIIN EEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHEEEEEHHHEEEE YYYKLNYKKNKKYQNLIYKYKKNKKEKSFKRKFIIMQESLISLKIYFLFKKNIKKKKREN EEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHCC FQLIDMSLLPILAEIENNGVLIKKKILKKQKKNHEKTLKKLKKKIFKITKEKFNINSSKQ CEEEEHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH LQNILFKKFNLPKIYKTKLGNISTNEIVLKKLSKIHILPKIILQFRLIKKIINTYLRNLI HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KSINNKTNRIHTTYNQINTSTGRLSSKNPNLQNIPIKTKIGRKLRIAFITKKKWLLLTAD HHHCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEEECCCEEEEEEC YSHIELRIIAHYSKDKNLIKDLLKNKDIHISTASHIFNKPLEKIKKYHRNIAKTINFSIL CCEEEEEEEEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEE YGISPFGLSKKLNISIKKSKNLIYRYFLKYNKIKRFIKNTYKTAKKRGYIKTLFKRKLYI EECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC PNINSKNIYLKNSAKRFCINLMIQNTASDIIKKSMITLHHLFKKKFPKDIKIIMQIHDEL CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH IFEIKEKKKDKIIDVIKKIMENNTKLIIPLYITTKIGKNWKEIKSI HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]