Click here to switch to the map view.

The map label for this gene is mfd [H]

Identifier: 116515116

GI number: 116515116

Start: 209599

End: 211650

Strand: Reverse

Name: mfd [H]

Synonym: BCc_180

Alternate gene names: 116515116

Gene position: 211650-209599 (Counterclockwise)

Preceding gene: 116515118

Following gene: 116515115

Centisome position: 50.83

GC content: 18.52

Gene sequence:

>2052_bases
ATGAAAAAAAAAAACACAAAAAAAAAAACATTTCAAAAAAATCAACTTGTAATTCATACTCAATACGGCATTGGTAGATA
TATTGATTTATGTACATTAAAAAATAAAGGAAAATTAACAGATTATTTTGTAATCATGTATGCAAATAAAGTTAAACTTT
ACGTACCTATTACATCTCTTAATTTAATAAATATTTATAAAACTTCAAATACTTCAAAAATATCTTTAAATACTTTAGGA
ACTAAAACATGGTTAGAAACATGTAAAAAAGTAAAAAAAAAAATTTATGATGCAGCTGTTGAATTAATTGATATTAAAGC
ACATCGATATTGTAGAAAAGGATTTTCCTTTAAAAAAAATATTTTGAAATACAAAAATTTTTGTAATAAGTGTTTGTATC
ATATAACACTTGATCAAAAAAACTCTATAAAAGAAATTATACAAGATATGAAAAAATCAGTTCCTATGGATCGTTTATTA
TGTGGAGATGTAGGATTTGGAAAAACTGAAATAGCTATGAGAGCTACTTTTATAGCTTTAGATAATAAAAAACAAGTTGC
TATTCTTGTTCCAACGACTTTATTAGCTCAACAACATTATAATACATTTAAAAATCGATTTTCTGAGTATAAGTATAAAA
TTAATGTATATTCACGTTTTACATCAAGTAAAAAAGAAAAAATAATTAAAAAAAAAATCAAAAATGGAAAAATAAATATT
CTTATTGGTACACATAAAATTTTATCAAAATATTTAATTTGGAAAAATTTAGGATTATTAATTATTGATGAAGAACATCG
TTTTGGTGTATTTCATAAAGAAAAAATAAAAAAATTATATATTAATATTGATATACTTACTTTAACAGCTACACCGATTC
CTAGAACATTAAATATGTCTATTCTTGGAATCAAAGATTTATCTATTATATCTACTCCGCCAAAAAAACGTTTAAAAATA
AAAACATTTATCAAAAATTTTCATCCTAAAATTATACGAAAAGTAATATTAAGAGAATTAAAAAGAAAAGGACAAGTATA
TTATTTATTTAATAGAGTTAAAAATATAGAAGAAAAAAAAAAATATTTATTATATTTAATCCCAGAAGCTCGTATTGAAA
TTAGTCATGGAAAAATGAATAGTAAAGATTTATATAAAATTATGTATGATTTTTTTAATAAAAAATTTGATATTTTAATC
TGTACTACAATTATAGATACTGAAATAAATATTAGTAATGTTAATACTATGATTATAGAAAATGCTGATAAATTTGGATT
ATCACAATTACATCAATTACGTGGTAGAATAGGTCGTTCTAAAAGACAAGCATATGCATTTTTTTTAATACCAAATTTAA
ATAAAATTAATAAAAAAGCAAAAAAACGATTAAATATAATAAAAAATTATACAGATTTAGGATCTGGTTTAATATTATCA
AAACATGATCTAGAAATAAGAGGAGTTGGTGAATTATTAGGAACAAATCAAAGCGGTCATATTAAAACAATTGGTTTTAA
ACTATATAAAAAATTTCTTAAAGAATCAATTTTAAAAATAAAGCAAAAAAAAAATTTACAAACAATTAATGAAATAAAAA
ATTTTAATCAAAATATTGATATTCAATTAAATGTATCTGCAATACTACCAAATAACTATATTAAAGATATTAATATAAGA
TTAATGTATTATAAAAAAATATCATCTGTTAAGAAAAACAAAAAACTAAAAAAAATAAAACATGCAATGTTTAATATATT
TGGAAAATTACCAAAATATACAAAAAATTTATTTTTATTAACACAAATAAAAATTCTTTCTAAAAAAATTGGTATTAAAA
AAATTTTTTTTCATATTAAAAAAATATGTTTAATTTTTTCAAAAAAAAATATTTTAAATATACAATGGTTATATAAAAAA
ATCATTAGAAATCTTTTTTATTGGAAAATATTAAAATATAAAATATATTATTATAAAAAATTTTCAAATGACAAAGAGTT
ATTATATTGGATTAAAAATTTTTTAAAAAAAATTATTAATAAAAATAAATAA

Upstream 100 bases:

>100_bases
ATAAAAATAAGAATATTGTTCTTTTTAAGTAAAAATAATAATATCTTTTTATATCAACTATATATTTATTATATATAATG
AATCGGAATAAATTTTTTTA

Downstream 100 bases:

>100_bases
TTTTAACTATTAATTTTTAATATTATTTATCAAATAAAATATTTTTAATTAAGGTAGATATGAAAAAAAAAATTCTTATT
TCATGTTCTAATAGTAAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: TRCF; ATP-dependent helicase mfd [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MKKKNTKKKTFQKNQLVIHTQYGIGRYIDLCTLKNKGKLTDYFVIMYANKVKLYVPITSLNLINIYKTSNTSKISLNTLG
TKTWLETCKKVKKKIYDAAVELIDIKAHRYCRKGFSFKKNILKYKNFCNKCLYHITLDQKNSIKEIIQDMKKSVPMDRLL
CGDVGFGKTEIAMRATFIALDNKKQVAILVPTTLLAQQHYNTFKNRFSEYKYKINVYSRFTSSKKEKIIKKKIKNGKINI
LIGTHKILSKYLIWKNLGLLIIDEEHRFGVFHKEKIKKLYINIDILTLTATPIPRTLNMSILGIKDLSIISTPPKKRLKI
KTFIKNFHPKIIRKVILRELKRKGQVYYLFNRVKNIEEKKKYLLYLIPEARIEISHGKMNSKDLYKIMYDFFNKKFDILI
CTTIIDTEINISNVNTMIIENADKFGLSQLHQLRGRIGRSKRQAYAFFLIPNLNKINKKAKKRLNIIKNYTDLGSGLILS
KHDLEIRGVGELLGTNQSGHIKTIGFKLYKKFLKESILKIKQKKNLQTINEIKNFNQNIDIQLNVSAILPNNYIKDINIR
LMYYKKISSVKKNKKLKKIKHAMFNIFGKLPKYTKNLFLLTQIKILSKKIGIKKIFFHIKKICLIFSKKNILNIQWLYKK
IIRNLFYWKILKYKIYYYKKFSNDKELLYWIKNFLKKIINKNK

Sequences:

>Translated_683_residues
MKKKNTKKKTFQKNQLVIHTQYGIGRYIDLCTLKNKGKLTDYFVIMYANKVKLYVPITSLNLINIYKTSNTSKISLNTLG
TKTWLETCKKVKKKIYDAAVELIDIKAHRYCRKGFSFKKNILKYKNFCNKCLYHITLDQKNSIKEIIQDMKKSVPMDRLL
CGDVGFGKTEIAMRATFIALDNKKQVAILVPTTLLAQQHYNTFKNRFSEYKYKINVYSRFTSSKKEKIIKKKIKNGKINI
LIGTHKILSKYLIWKNLGLLIIDEEHRFGVFHKEKIKKLYINIDILTLTATPIPRTLNMSILGIKDLSIISTPPKKRLKI
KTFIKNFHPKIIRKVILRELKRKGQVYYLFNRVKNIEEKKKYLLYLIPEARIEISHGKMNSKDLYKIMYDFFNKKFDILI
CTTIIDTEINISNVNTMIIENADKFGLSQLHQLRGRIGRSKRQAYAFFLIPNLNKINKKAKKRLNIIKNYTDLGSGLILS
KHDLEIRGVGELLGTNQSGHIKTIGFKLYKKFLKESILKIKQKKNLQTINEIKNFNQNIDIQLNVSAILPNNYIKDINIR
LMYYKKISSVKKNKKLKKIKHAMFNIFGKLPKYTKNLFLLTQIKILSKKIGIKKIFFHIKKICLIFSKKNILNIQWLYKK
IIRNLFYWKILKYKIYYYKKFSNDKELLYWIKNFLKKIINKNK
>Mature_683_residues
MKKKNTKKKTFQKNQLVIHTQYGIGRYIDLCTLKNKGKLTDYFVIMYANKVKLYVPITSLNLINIYKTSNTSKISLNTLG
TKTWLETCKKVKKKIYDAAVELIDIKAHRYCRKGFSFKKNILKYKNFCNKCLYHITLDQKNSIKEIIQDMKKSVPMDRLL
CGDVGFGKTEIAMRATFIALDNKKQVAILVPTTLLAQQHYNTFKNRFSEYKYKINVYSRFTSSKKEKIIKKKIKNGKINI
LIGTHKILSKYLIWKNLGLLIIDEEHRFGVFHKEKIKKLYINIDILTLTATPIPRTLNMSILGIKDLSIISTPPKKRLKI
KTFIKNFHPKIIRKVILRELKRKGQVYYLFNRVKNIEEKKKYLLYLIPEARIEISHGKMNSKDLYKIMYDFFNKKFDILI
CTTIIDTEINISNVNTMIIENADKFGLSQLHQLRGRIGRSKRQAYAFFLIPNLNKINKKAKKRLNIIKNYTDLGSGLILS
KHDLEIRGVGELLGTNQSGHIKTIGFKLYKKFLKESILKIKQKKNLQTINEIKNFNQNIDIQLNVSAILPNNYIKDINIR
LMYYKKISSVKKNKKLKKIKHAMFNIFGKLPKYTKNLFLLTQIKILSKKIGIKKIFFHIKKICLIFSKKNILNIQWLYKK
IIRNLFYWKILKYKIYYYKKFSNDKELLYWIKNFLKKIINKNK

Specific function: Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the

COG id: COG1197

COG function: function code LK; Transcription-repair coupling factor (superfamily II helicase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1787357, Length=668, Percent_Identity=44.9101796407186, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI2367254, Length=381, Percent_Identity=34.1207349081365, Blast_Score=228, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003711
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR004576
- InterPro:   IPR005118 [H]

Pfam domain/function: PF02559 CarD_TRCF; PF00270 DEAD; PF00271 Helicase_C; PF03461 TRCF [H]

EC number: NA

Molecular weight: Translated: 80724; Mature: 80724

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNTKKKTFQKNQLVIHTQYGIGRYIDLCTLKNKGKLTDYFVIMYANKVKLYVPITSL
CCCCCCCHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEE
NLINIYKTSNTSKISLNTLGTKTWLETCKKVKKKIYDAAVELIDIKAHRYCRKGFSFKKN
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ILKYKNFCNKCLYHITLDQKNSIKEIIQDMKKSVPMDRLLCGDVGFGKTEIAMRATFIAL
HHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCHHHEEECCCCCCCHHHEEEEEEEEE
DNKKQVAILVPTTLLAQQHYNTFKNRFSEYKYKINVYSRFTSSKKEKIIKKKIKNGKINI
CCCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEHHCCHHHHHHHHHHHCCCEEEE
LIGTHKILSKYLIWKNLGLLIIDEEHRFGVFHKEKIKKLYINIDILTLTATPIPRTLNMS
EEECHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCEEEEEEEEEEEEECCCCCCCCCEE
ILGIKDLSIISTPPKKRLKIKTFIKNFHPKIIRKVILRELKRKGQVYYLFNRVKNIEEKK
EEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHC
KYLLYLIPEARIEISHGKMNSKDLYKIMYDFFNKKFDILICTTIIDTEINISNVNTMIIE
CEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECEEEECCCEEEEEE
NADKFGLSQLHQLRGRIGRSKRQAYAFFLIPNLNKINKKAKKRLNIIKNYTDLGSGLILS
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
KHDLEIRGVGELLGTNQSGHIKTIGFKLYKKFLKESILKIKQKKNLQTINEIKNFNQNID
ECCCEEEECHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
IQLNVSAILPNNYIKDINIRLMYYKKISSVKKNKKLKKIKHAMFNIFGKLPKYTKNLFLL
EEEEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEE
TQIKILSKKIGIKKIFFHIKKICLIFSKKNILNIQWLYKKIIRNLFYWKILKYKIYYYKK
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEEEEE
FSNDKELLYWIKNFLKKIINKNK
CCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKNTKKKTFQKNQLVIHTQYGIGRYIDLCTLKNKGKLTDYFVIMYANKVKLYVPITSL
CCCCCCCHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEE
NLINIYKTSNTSKISLNTLGTKTWLETCKKVKKKIYDAAVELIDIKAHRYCRKGFSFKKN
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ILKYKNFCNKCLYHITLDQKNSIKEIIQDMKKSVPMDRLLCGDVGFGKTEIAMRATFIAL
HHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCHHHEEECCCCCCCHHHEEEEEEEEE
DNKKQVAILVPTTLLAQQHYNTFKNRFSEYKYKINVYSRFTSSKKEKIIKKKIKNGKINI
CCCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEHHCCHHHHHHHHHHHCCCEEEE
LIGTHKILSKYLIWKNLGLLIIDEEHRFGVFHKEKIKKLYINIDILTLTATPIPRTLNMS
EEECHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCEEEEEEEEEEEEECCCCCCCCCEE
ILGIKDLSIISTPPKKRLKIKTFIKNFHPKIIRKVILRELKRKGQVYYLFNRVKNIEEKK
EEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHC
KYLLYLIPEARIEISHGKMNSKDLYKIMYDFFNKKFDILICTTIIDTEINISNVNTMIIE
CEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECEEEECCCEEEEEE
NADKFGLSQLHQLRGRIGRSKRQAYAFFLIPNLNKINKKAKKRLNIIKNYTDLGSGLILS
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
KHDLEIRGVGELLGTNQSGHIKTIGFKLYKKFLKESILKIKQKKNLQTINEIKNFNQNID
ECCCEEEECHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
IQLNVSAILPNNYIKDINIRLMYYKKISSVKKNKKLKKIKHAMFNIFGKLPKYTKNLFLL
EEEEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEE
TQIKILSKKIGIKKIFFHIKKICLIFSKKNILNIQWLYKKIIRNLFYWKILKYKIYYYKK
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEEEEE
FSNDKELLYWIKNFLKKIINKNK
CCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8465200; 8905232; 9278503 [H]