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The map label for this gene is lepA

Identifier: 116515101

GI number: 116515101

Start: 190867

End: 192657

Strand: Reverse

Name: lepA

Synonym: BCc_165

Alternate gene names: 116515101

Gene position: 192657-190867 (Counterclockwise)

Preceding gene: 116515107

Following gene: 116515100

Centisome position: 46.27

GC content: 22.56

Gene sequence:

>1791_bases
ATGAAACATATAAGAAATTTTTCTATTATTGCACACATAGATCATGGAAAATCTACTTTATCTGATCGATTAATACAAAA
ATGTGGAGGTTTATCTGAAAGAGAAATGTCAGAACAAGTATTAGATACTATGGATTTAGAAAAGGAACGTGGAATTACAA
TTAAAGCTCAAAGTGTAACAATTTCATATATTTCTAAAAAAGGAAAAAAATATAGTTTAAATTTTATTGACACTCCTGGA
CACGTAAATTTTGCATATGAAGTATCACGATCTTTATCAGCATGTGAAGGTGCTTTATTAGTAATTGATTCAGTTAAAGG
GGTACAAGCTCAAACAGTTGCAAATTGCCATAGTGCTATAAAAATGAAATTATCCATTTTACCTGTTTTAAATAAAATTG
ATTTACCAAATTCTCAACCAGAAAAAGTATGTAGAGATATTAAAAAAATCATAGGTATATCAACAAAAAATATTGCCTTA
TGTTCTGCAAAAACAGGTAAGGGAATAACTGAATTATTAGAAAAAATAATTCAGGTTATACCTTCGCCTACAGGTGATAT
TAATAATTCTTTACAAGCATTAGTTATAGATTCTTGGTTTGATAAATATTCAGGTGTAGTATTATTAATAAGAATTAAAA
ACGGATTAATAAAAATAAAAGATAAGATAAAAATAATAGGAAAAAAAAATATATATATAGTTGAACAACTAGGAATATTT
ACACCTAAAAAAATCAAAAAAAAAAAATTATATTGCGAAGAAGTAGGTTGGGTAATATGTGGAATCAAAAACATTTATTC
AATATCAGTAGGTGAAACAATTACTACGTTTATTAATTCTTCAAAAAAAAAATTATCTCATTTTAAAAAAATTACACCTA
AAATTTATGCGGGTTTATTTCCATTAAAAAACAATCAATATAATAATTTTAAAAATGCTTTAAATAAACTTCAATTAAAT
GATTCTTCTTTATTTTATGAACCAGAAAATTCACAAGCTTTAGGATTTGGTTTTCGATGTGGATTTCTAGGAACATTACA
TATGGAGATTATTCAATCTCGTTTAGAAAGAGAATATAATATAGAAATAATCTTAACATCACCTACCGTTGTTTATGAAG
TTGTTTTAAAAAAAAACAAAAAAAAAATTTATTTTAATAATCCAACAAAATTTCCAAAAAATAATTTAATTAAAAAAATT
AAAGAACCCATAGCACGTTGTAATATTTTAACTCCAAATAAATATATTGGAGAAATTATAAAGTTATGTATTAAAAAAAG
AGGTATACAAAAAAATTTAATATATCATGAAAAAAAAACTATTTTAATATATGAAATTCCGTTATCTGAAGTAATTTCAA
ATTTTTTTGATAAATTAAAATCTATATCTAGTGGATATGCTTCGTTAGATTATTATTTTATTGGATTTAAAACAAGTAAT
TTAGTAAAAATTGATATTTTAATTAATTTTACAAAAATTGATGCACTTTCATATATATGTCATAAAAATGATTCTCAAAA
AATAGCTAAAAGGATTATTGAAAAAATAAAAAAAATAATACCAAGACATCAATTTCATGTTCCTATTCAAGCAGCTATTG
AAAATAATATTATTTCTAGAGTAAATATTACACAATTAAGAAAAAATGTATTATCTAAATGTTACGGAGGTGATATTAGC
CGAAAAAAAAAATTATTACAAAAACAAAAAAAAGGAAAAAAAAGAATGAAGATAATTGGAAATATTAAAATCCCTCAAGA
AGTTTTTTTTACAATATTAAAAATAAATTAA

Upstream 100 bases:

>100_bases
TCTTAATTAATATATTAATATTAATATTAAAAATATTTATTTTTTATAAAAAAAATTTTTTTTAAATTAAAATTAAATTA
AATGGTGAAAAAATTACACA

Downstream 100 bases:

>100_bases
TCAATATTAGGAATAATATAATGAAAATTATAATAAGAAAAATATTATTATTATTATCATTAATAATAATATTTTTATTA
ATTATAAATTTTATAAAAAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSEQVLDTMDLEKERGITIKAQSVTISYISKKGKKYSLNFIDTPG
HVNFAYEVSRSLSACEGALLVIDSVKGVQAQTVANCHSAIKMKLSILPVLNKIDLPNSQPEKVCRDIKKIIGISTKNIAL
CSAKTGKGITELLEKIIQVIPSPTGDINNSLQALVIDSWFDKYSGVVLLIRIKNGLIKIKDKIKIIGKKNIYIVEQLGIF
TPKKIKKKKLYCEEVGWVICGIKNIYSISVGETITTFINSSKKKLSHFKKITPKIYAGLFPLKNNQYNNFKNALNKLQLN
DSSLFYEPENSQALGFGFRCGFLGTLHMEIIQSRLEREYNIEIILTSPTVVYEVVLKKNKKKIYFNNPTKFPKNNLIKKI
KEPIARCNILTPNKYIGEIIKLCIKKRGIQKNLIYHEKKTILIYEIPLSEVISNFFDKLKSISSGYASLDYYFIGFKTSN
LVKIDILINFTKIDALSYICHKNDSQKIAKRIIEKIKKIIPRHQFHVPIQAAIENNIISRVNITQLRKNVLSKCYGGDIS
RKKKLLQKQKKGKKRMKIIGNIKIPQEVFFTILKIN

Sequences:

>Translated_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSEQVLDTMDLEKERGITIKAQSVTISYISKKGKKYSLNFIDTPG
HVNFAYEVSRSLSACEGALLVIDSVKGVQAQTVANCHSAIKMKLSILPVLNKIDLPNSQPEKVCRDIKKIIGISTKNIAL
CSAKTGKGITELLEKIIQVIPSPTGDINNSLQALVIDSWFDKYSGVVLLIRIKNGLIKIKDKIKIIGKKNIYIVEQLGIF
TPKKIKKKKLYCEEVGWVICGIKNIYSISVGETITTFINSSKKKLSHFKKITPKIYAGLFPLKNNQYNNFKNALNKLQLN
DSSLFYEPENSQALGFGFRCGFLGTLHMEIIQSRLEREYNIEIILTSPTVVYEVVLKKNKKKIYFNNPTKFPKNNLIKKI
KEPIARCNILTPNKYIGEIIKLCIKKRGIQKNLIYHEKKTILIYEIPLSEVISNFFDKLKSISSGYASLDYYFIGFKTSN
LVKIDILINFTKIDALSYICHKNDSQKIAKRIIEKIKKIIPRHQFHVPIQAAIENNIISRVNITQLRKNVLSKCYGGDIS
RKKKLLQKQKKGKKRMKIIGNIKIPQEVFFTILKIN
>Mature_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSEQVLDTMDLEKERGITIKAQSVTISYISKKGKKYSLNFIDTPG
HVNFAYEVSRSLSACEGALLVIDSVKGVQAQTVANCHSAIKMKLSILPVLNKIDLPNSQPEKVCRDIKKIIGISTKNIAL
CSAKTGKGITELLEKIIQVIPSPTGDINNSLQALVIDSWFDKYSGVVLLIRIKNGLIKIKDKIKIIGKKNIYIVEQLGIF
TPKKIKKKKLYCEEVGWVICGIKNIYSISVGETITTFINSSKKKLSHFKKITPKIYAGLFPLKNNQYNNFKNALNKLQLN
DSSLFYEPENSQALGFGFRCGFLGTLHMEIIQSRLEREYNIEIILTSPTVVYEVVLKKNKKKIYFNNPTKFPKNNLIKKI
KEPIARCNILTPNKYIGEIIKLCIKKRGIQKNLIYHEKKTILIYEIPLSEVISNFFDKLKSISSGYASLDYYFIGFKTSN
LVKIDILINFTKIDALSYICHKNDSQKIAKRIIEKIKKIIPRHQFHVPIQAAIENNIISRVNITQLRKNVLSKCYGGDIS
RKKKLLQKQKKGKKRMKIIGNIKIPQEVFFTILKIN

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=45.0166112956811, Blast_Score=556, Evalue=1e-158,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=39.8496240601504, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI18390331, Length=183, Percent_Identity=33.879781420765, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI25306283, Length=177, Percent_Identity=35.5932203389831, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=41.7910447761194, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=41.7910447761194, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=36.0902255639098, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=36.0902255639098, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=37.6146788990826, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=37.6146788990826, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=37.6146788990826, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=27.8969957081545, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=27.8969957081545, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=58.8926174496644, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=507, Percent_Identity=25.6410256410256, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=35.8024691358025, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790835, Length=177, Percent_Identity=29.3785310734463, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790412, Length=278, Percent_Identity=24.4604316546763, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789737, Length=278, Percent_Identity=24.4604316546763, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=25.8771929824561, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=37.5409836065574, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.3057324840764, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=188, Percent_Identity=35.1063829787234, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556745, Length=153, Percent_Identity=33.9869281045752, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=37.3134328358209, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=37.3134328358209, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=40.6301824212272, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=39.5833333333333, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=39.5833333333333, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=181, Percent_Identity=33.7016574585635, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=37.5886524822695, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=23.1316725978648, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322675, Length=144, Percent_Identity=31.9444444444444, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=39.8671096345515, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=37.5838926174497, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=37.5838926174497, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=35.7142857142857, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=37.5838926174497, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=41.2162162162162, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=40.5797101449275, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=37.7777777777778, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281363316, Length=279, Percent_Identity=22.5806451612903, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864358, Length=279, Percent_Identity=22.5806451612903, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921738, Length=280, Percent_Identity=28.9285714285714, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_BUCCC (Q057R2)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802730.1
- ProteinModelPortal:   Q057R2
- SMR:   Q057R2
- STRING:   Q057R2
- EnsemblBacteria:   EBBUCT00000001495
- GeneID:   4441012
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_165
- eggNOG:   COG0481
- GeneTree:   EBGT00050000007783
- HOGENOM:   HBG286375
- OMA:   VQKREMK
- ProtClustDB:   PRK05433
- BioCyc:   BAPH372461:BCC_165-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67811; Mature: 67811

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSEQVLDTMDLEKERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEEEE
ISYISKKGKKYSLNFIDTPGHVNFAYEVSRSLSACEGALLVIDSVKGVQAQTVANCHSAI
EEEHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KMKLSILPVLNKIDLPNSQPEKVCRDIKKIIGISTKNIALCSAKTGKGITELLEKIIQVI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHC
PSPTGDINNSLQALVIDSWFDKYSGVVLLIRIKNGLIKIKDKIKIIGKKNIYIVEQLGIF
CCCCCCCCCCHHEEEEHHHHHCCCCEEEEEEECCCEEEEECEEEEEECCCEEEEEECCCC
TPKKIKKKKLYCEEVGWVICGIKNIYSISVGETITTFINSSKKKLSHFKKITPKIYAGLF
CCHHHHHHHHHHHHCCEEEEEHHHHEEEECCHHHHHHHCCHHHHHHHHHHCCHHHHHCCC
PLKNNQYNNFKNALNKLQLNDSSLFYEPENSQALGFGFRCGFLGTLHMEIIQSRLEREYN
CCCCCCCCHHHHHHHHEEECCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCC
IEIILTSPTVVYEVVLKKNKKKIYFNNPTKFPKNNLIKKIKEPIARCNILTPNKYIGEII
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KLCIKKRGIQKNLIYHEKKTILIYEIPLSEVISNFFDKLKSISSGYASLDYYFIGFKTSN
HHHHHHCCCHHHHEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCC
LVKIDILINFTKIDALSYICHKNDSQKIAKRIIEKIKKIIPRHQFHVPIQAAIENNIISR
EEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCCHHH
VNITQLRKNVLSKCYGGDISRKKKLLQKQKKGKKRMKIIGNIKIPQEVFFTILKIN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCHHHEEEEEECC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSEQVLDTMDLEKERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEEEE
ISYISKKGKKYSLNFIDTPGHVNFAYEVSRSLSACEGALLVIDSVKGVQAQTVANCHSAI
EEEHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KMKLSILPVLNKIDLPNSQPEKVCRDIKKIIGISTKNIALCSAKTGKGITELLEKIIQVI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHC
PSPTGDINNSLQALVIDSWFDKYSGVVLLIRIKNGLIKIKDKIKIIGKKNIYIVEQLGIF
CCCCCCCCCCHHEEEEHHHHHCCCCEEEEEEECCCEEEEECEEEEEECCCEEEEEECCCC
TPKKIKKKKLYCEEVGWVICGIKNIYSISVGETITTFINSSKKKLSHFKKITPKIYAGLF
CCHHHHHHHHHHHHCCEEEEEHHHHEEEECCHHHHHHHCCHHHHHHHHHHCCHHHHHCCC
PLKNNQYNNFKNALNKLQLNDSSLFYEPENSQALGFGFRCGFLGTLHMEIIQSRLEREYN
CCCCCCCCHHHHHHHHEEECCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCC
IEIILTSPTVVYEVVLKKNKKKIYFNNPTKFPKNNLIKKIKEPIARCNILTPNKYIGEII
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KLCIKKRGIQKNLIYHEKKTILIYEIPLSEVISNFFDKLKSISSGYASLDYYFIGFKTSN
HHHHHHCCCHHHHEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCC
LVKIDILINFTKIDALSYICHKNDSQKIAKRIIEKIKKIIPRHQFHVPIQAAIENNIISR
EEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCCHHH
VNITQLRKNVLSKCYGGDISRKKKLLQKQKKGKKRMKIIGNIKIPQEVFFTILKIN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA