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The map label for this gene is aceF [H]

Identifier: 116515072

GI number: 116515072

Start: 155222

End: 156475

Strand: Direct

Name: aceF [H]

Synonym: BCc_132

Alternate gene names: 116515072

Gene position: 155222-156475 (Clockwise)

Preceding gene: 116515071

Following gene: 116515073

Centisome position: 37.28

GC content: 21.61

Gene sequence:

>1254_bases
GTGGACGTAGAAGTTCGTGTACCAGATATTGGAATTAAAGATGTTGAAGTAATTGAAATTTTCGTAAAAAAAGGAGATAT
AGTTAGTAAAGAAGATAGTTTAATTTCTGTTGAAGGACATAAATCAGTTCTTGAAATTCCATCTCCTATTTCTGGTATAA
TAAAAAAAATATGTACACAAGTAGGAGATAAATTATCTATTGATAAATTAATTTTAATAATTAATAATAATCAAGAGAAT
AATATTTCTAAAAAAAATAATAAAGATTATATTCAAAATAGTATTGATTATTTAAATCATACAAATAATAATAATAAAGA
CATAAAATATTTAAACGAAAAAAATAATAAAAATATTCATGCATCTCCTTATATTCGTCGTTTTGCAAGAATTTTAGATA
TTAATTTGTTATATATAAATGGAAGTGGAAAAAAAGGAAGAATTGTAAAAAAAGATATAGAAAAATATGATTTTTTAAAT
AAAAATATAAATAATAATTTTATAGAAAAGAATAACTGTTTAACAGAATTGAATACAAAGAATATAGATAATAAATCTAT
AAATCAACCATTAACTAGGATTCAAAGAATTTCTGGTAAAAATTTATTAAATAATTGGAATAATATTCCACATGTTACTC
AATTCGATGAAGCAGATATAACTGAATTAGAAGATTTTAGAAAATCATATAATTTAAATCAATTAAATAAAAATAAATCT
TTTCAAAAGGTTTCGTTATTATCGTTTTTAGTTAAATCTGTAATACATGCTTTATTAAAATATCCTAGATTTAATAGTAT
TTTAGATAAAAGTAAAAAAAATATAATTATTAAAAAAGATATTAATATTGGTATAGCTGTTGATACTCATGATGGTTTAT
TAGTACCAGTTTTAAAAAGTTTAAAAAATAAAACTATTTATGAAATATCAAATAATATTTTTAATGTAGTTACAAAAACT
AAAAATAATCAATTATGCACCTCGGAAATGACTGATGGTAGTTTTACTATTTCTAGTTTAGGAGGAATTGGTGGTATTGG
ATTTACACCAATTATTAATGCTCCAGAAGTTTGTATATTAGGTATCTCAAAAGCAGATATAAAACCTGTTTGGAATAAAA
AAAAATTTTATCCTCGTTTAATTCTTCCTTTTTCTATATCATATGATCATCGTGTAATAGATGGTGCTGACGGCGTTCGT
TTTACTACTTTTTTAAAAGATATATTATCTGATATTAGGATATTGTTAATGTAG

Upstream 100 bases:

>100_bases
TACAAGGTGATATAGATTCTAGTATATTTTTAGATGCATTAAAAAAATTTAATATTTTTTCAAATAAATTGAATCCACGT
TTAGCATAAGAGGTGATATA

Downstream 100 bases:

>100_bases
TAATAAAGATTTTTATGTTAAATTATAAATATTTAATTTTATTAAAAAAAAATAAAATATTTCTATATAAAGAGAAATTT
AATTTATGAATAAAAATATT

Product: hypothetical protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MDVEVRVPDIGIKDVEVIEIFVKKGDIVSKEDSLISVEGHKSVLEIPSPISGIIKKICTQVGDKLSIDKLILIINNNQEN
NISKKNNKDYIQNSIDYLNHTNNNNKDIKYLNEKNNKNIHASPYIRRFARILDINLLYINGSGKKGRIVKKDIEKYDFLN
KNINNNFIEKNNCLTELNTKNIDNKSINQPLTRIQRISGKNLLNNWNNIPHVTQFDEADITELEDFRKSYNLNQLNKNKS
FQKVSLLSFLVKSVIHALLKYPRFNSILDKSKKNIIIKKDINIGIAVDTHDGLLVPVLKSLKNKTIYEISNNIFNVVTKT
KNNQLCTSEMTDGSFTISSLGGIGGIGFTPIINAPEVCILGISKADIKPVWNKKKFYPRLILPFSISYDHRVIDGADGVR
FTTFLKDILSDIRILLM

Sequences:

>Translated_417_residues
MDVEVRVPDIGIKDVEVIEIFVKKGDIVSKEDSLISVEGHKSVLEIPSPISGIIKKICTQVGDKLSIDKLILIINNNQEN
NISKKNNKDYIQNSIDYLNHTNNNNKDIKYLNEKNNKNIHASPYIRRFARILDINLLYINGSGKKGRIVKKDIEKYDFLN
KNINNNFIEKNNCLTELNTKNIDNKSINQPLTRIQRISGKNLLNNWNNIPHVTQFDEADITELEDFRKSYNLNQLNKNKS
FQKVSLLSFLVKSVIHALLKYPRFNSILDKSKKNIIIKKDINIGIAVDTHDGLLVPVLKSLKNKTIYEISNNIFNVVTKT
KNNQLCTSEMTDGSFTISSLGGIGGIGFTPIINAPEVCILGISKADIKPVWNKKKFYPRLILPFSISYDHRVIDGADGVR
FTTFLKDILSDIRILLM
>Mature_417_residues
MDVEVRVPDIGIKDVEVIEIFVKKGDIVSKEDSLISVEGHKSVLEIPSPISGIIKKICTQVGDKLSIDKLILIINNNQEN
NISKKNNKDYIQNSIDYLNHTNNNNKDIKYLNEKNNKNIHASPYIRRFARILDINLLYINGSGKKGRIVKKDIEKYDFLN
KNINNNFIEKNNCLTELNTKNIDNKSINQPLTRIQRISGKNLLNNWNNIPHVTQFDEADITELEDFRKSYNLNQLNKNKS
FQKVSLLSFLVKSVIHALLKYPRFNSILDKSKKNIIIKKDINIGIAVDTHDGLLVPVLKSLKNKTIYEISNNIFNVVTKT
KNNQLCTSEMTDGSFTISSLGGIGGIGFTPIINAPEVCILGISKADIKPVWNKKKFYPRLILPFSISYDHRVIDGADGVR
FTTFLKDILSDIRILLM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=30.1624129930394, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=32.2869955156951, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI31711992, Length=304, Percent_Identity=29.2763157894737, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI203098816, Length=234, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI203098753, Length=234, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=128, Percent_Identity=34.375, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786305, Length=424, Percent_Identity=42.2169811320755, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=27.2076372315036, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=28.0660377358491, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=227, Percent_Identity=31.7180616740088, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=426, Percent_Identity=25.3521126760563, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538894, Length=294, Percent_Identity=25.8503401360544, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=22.8855721393035, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324258, Length=314, Percent_Identity=27.0700636942675, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=28.537170263789, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24582497, Length=420, Percent_Identity=24.5238095238095, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20129315, Length=420, Percent_Identity=24.5238095238095, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24645909, Length=203, Percent_Identity=30.5418719211823, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47425; Mature: 47425

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEVRVPDIGIKDVEVIEIFVKKGDIVSKEDSLISVEGHKSVLEIPSPISGIIKKICTQ
CCCEEECCCCCCCCHHEEEEEECCCCEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHH
VGDKLSIDKLILIINNNQENNISKKNNKDYIQNSIDYLNHTNNNNKDIKYLNEKNNKNIH
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEE
ASPYIRRFARILDINLLYINGSGKKGRIVKKDIEKYDFLNKNINNNFIEKNNCLTELNTK
CCHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
NIDNKSINQPLTRIQRISGKNLLNNWNNIPHVTQFDEADITELEDFRKSYNLNQLNKNKS
CCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
FQKVSLLSFLVKSVIHALLKYPRFNSILDKSKKNIIIKKDINIGIAVDTHDGLLVPVLKS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHH
LKNKTIYEISNNIFNVVTKTKNNQLCTSEMTDGSFTISSLGGIGGIGFTPIINAPEVCIL
HCCCEEEEHHCCCEEEEEECCCCCEEECCCCCCCEEHHHCCCCCCCCCCCCCCCCCEEEE
GISKADIKPVWNKKKFYPRLILPFSISYDHRVIDGADGVRFTTFLKDILSDIRILLM
ECCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVEVRVPDIGIKDVEVIEIFVKKGDIVSKEDSLISVEGHKSVLEIPSPISGIIKKICTQ
CCCEEECCCCCCCCHHEEEEEECCCCEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHH
VGDKLSIDKLILIINNNQENNISKKNNKDYIQNSIDYLNHTNNNNKDIKYLNEKNNKNIH
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEE
ASPYIRRFARILDINLLYINGSGKKGRIVKKDIEKYDFLNKNINNNFIEKNNCLTELNTK
CCHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
NIDNKSINQPLTRIQRISGKNLLNNWNNIPHVTQFDEADITELEDFRKSYNLNQLNKNKS
CCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
FQKVSLLSFLVKSVIHALLKYPRFNSILDKSKKNIIIKKDINIGIAVDTHDGLLVPVLKS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHH
LKNKTIYEISNNIFNVVTKTKNNQLCTSEMTDGSFTISSLGGIGGIGFTPIINAPEVCIL
HCCCEEEEHHCCCEEEEEECCCCCEEECCCCCCCEEHHHCCCCCCCCCCCCCCCCCEEEE
GISKADIKPVWNKKKFYPRLILPFSISYDHRVIDGADGVRFTTFLKDILSDIRILLM
ECCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]