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The map label for this gene is lpdA [H]

Identifier: 116515073

GI number: 116515073

Start: 156561

End: 157988

Strand: Direct

Name: lpdA [H]

Synonym: BCc_133

Alternate gene names: 116515073

Gene position: 156561-157988 (Clockwise)

Preceding gene: 116515072

Following gene: 116515082

Centisome position: 37.6

GC content: 25.0

Gene sequence:

>1428_bases
ATGAATAAAAATATTAATGTTAATGTAGTTGTTATTGGCGGAGGTCCGGCTGGATATTCCGCAGCATTTAGATGTTCTGA
TTTAGGATTTTCTACTTTAATAATAGAAAAATATGGAATTTTAGGAGGAACTTGTTTAAATGTAGGATGTATTCCATCTA
AATCTTTTTTATATTTAGCTACTTTAATAAAAGAAATAAAAGAATTTTCTAAATATGGTATTGATTTATGTGCTAATAAT
AAAATTGATATATCAAAAATTATGAAATGGAAAGATAATATTGTTTTAGAGTTAAGTAATGGATTAAAAAATATGGCAGA
ATATAGAAAAATTGATATATTAAAAGGAGTAGCTAAATTTTTTAAAAAAAATCAATTAAAAGTAATTTTAAATAATGGAA
AAATATCTTATATAAAATATGATTATGCTATTATTGCTACAGGTTCTAAACCTATTACTTTATCAAATTTTTTTAGTAAA
GATTCTCGTATTTGGAATTCTACACATGCTTTAAATTCTGATTTTATTCCTAAAAAATTGTTAATTATAGGTGCAGGTAT
CATTGGTTTAGAAATGGCAACTATTTATAGTACTTTAGGATCTGAAGTAGATATAATTGATAATTCTGAAAAATTTTTTC
CGTTAATTGATAAAGATGTTAGTGATGTTTTTTTAAAATACATTCAGAAATATTTTTCTATATCATTAAATACATCAATA
CTTAGTTCTATTTCTGATAAAGATGGTATTTTAGTAAACACTAAAATAAATCATAATACTTATAATAGTAAAATGTATCA
TGCAATTTTAATAGCAGTTGGTAGAACTGCGAATACAGATTGTTTAGATATTAACATACCTGGTATAAAAGTAGATAAAT
ATGGTTTTATTCAAGTAGATAATCAAATGCGTACTAATATTTCTAATATTTTTGCTATAGGAGATGTAGTTGGTCAACCT
ATGTTAGCGCATAAAGGTATGCATGAAGCACATATAGCAGCTGAAGTTATTGCTGGAAAAAAACATTTTTTTGATCCTAA
AGTAATTCCATGTGTAGCTTATTGTGATCCTGAAATTGCTTGGACAGGAATTATGGAAGAAGAAGCTAAAAATAAAGGAA
TTAACTGTCGTTCAATAATTTTTCCTTGGAAATTTTTAGGAAAAGCAATAAGTTCTAATTGTTCTATTCCTGGTCTAACA
AAATTAATTGTTGATACAAATAGTAATAGAATTATTGGAGGGGTTATTATTGGTAAACATGCAGGAGAATTATTATCACA
AATTAACTTATCTATTGAAATGGGATGTGATATAGAAGATATAGCTTTAACAATCCATCCTCATCCTTCTTTATCTGAAT
CAATTAATATATCAGCACAATTATTTAATGGAACTGCTACTGATATGATTAATAAAAATATTATATAA

Upstream 100 bases:

>100_bases
ATATTGTTAATGTAGTAATAAAGATTTTTATGTTAAATTATAAATATTTAATTTTATTAAAAAAAAATAAAATATTTCTA
TATAAAGAGAAATTTAATTT

Downstream 100 bases:

>100_bases
ATTAAAAATTTTTAAGATATTAATTTTATTAATATATTTCTAAGATTTTTTTTTAATAAAATTAAAATTTATTAATATAT
TTATATTTAGATATTTATAA

Product: LpdA

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNKNINVNVVVIGGGPAGYSAAFRCSDLGFSTLIIEKYGILGGTCLNVGCIPSKSFLYLATLIKEIKEFSKYGIDLCANN
KIDISKIMKWKDNIVLELSNGLKNMAEYRKIDILKGVAKFFKKNQLKVILNNGKISYIKYDYAIIATGSKPITLSNFFSK
DSRIWNSTHALNSDFIPKKLLIIGAGIIGLEMATIYSTLGSEVDIIDNSEKFFPLIDKDVSDVFLKYIQKYFSISLNTSI
LSSISDKDGILVNTKINHNTYNSKMYHAILIAVGRTANTDCLDINIPGIKVDKYGFIQVDNQMRTNISNIFAIGDVVGQP
MLAHKGMHEAHIAAEVIAGKKHFFDPKVIPCVAYCDPEIAWTGIMEEEAKNKGINCRSIIFPWKFLGKAISSNCSIPGLT
KLIVDTNSNRIIGGVIIGKHAGELLSQINLSIEMGCDIEDIALTIHPHPSLSESINISAQLFNGTATDMINKNII

Sequences:

>Translated_475_residues
MNKNINVNVVVIGGGPAGYSAAFRCSDLGFSTLIIEKYGILGGTCLNVGCIPSKSFLYLATLIKEIKEFSKYGIDLCANN
KIDISKIMKWKDNIVLELSNGLKNMAEYRKIDILKGVAKFFKKNQLKVILNNGKISYIKYDYAIIATGSKPITLSNFFSK
DSRIWNSTHALNSDFIPKKLLIIGAGIIGLEMATIYSTLGSEVDIIDNSEKFFPLIDKDVSDVFLKYIQKYFSISLNTSI
LSSISDKDGILVNTKINHNTYNSKMYHAILIAVGRTANTDCLDINIPGIKVDKYGFIQVDNQMRTNISNIFAIGDVVGQP
MLAHKGMHEAHIAAEVIAGKKHFFDPKVIPCVAYCDPEIAWTGIMEEEAKNKGINCRSIIFPWKFLGKAISSNCSIPGLT
KLIVDTNSNRIIGGVIIGKHAGELLSQINLSIEMGCDIEDIALTIHPHPSLSESINISAQLFNGTATDMINKNII
>Mature_475_residues
MNKNINVNVVVIGGGPAGYSAAFRCSDLGFSTLIIEKYGILGGTCLNVGCIPSKSFLYLATLIKEIKEFSKYGIDLCANN
KIDISKIMKWKDNIVLELSNGLKNMAEYRKIDILKGVAKFFKKNQLKVILNNGKISYIKYDYAIIATGSKPITLSNFFSK
DSRIWNSTHALNSDFIPKKLLIIGAGIIGLEMATIYSTLGSEVDIIDNSEKFFPLIDKDVSDVFLKYIQKYFSISLNTSI
LSSISDKDGILVNTKINHNTYNSKMYHAILIAVGRTANTDCLDINIPGIKVDKYGFIQVDNQMRTNISNIFAIGDVVGQP
MLAHKGMHEAHIAAEVIAGKKHFFDPKVIPCVAYCDPEIAWTGIMEEEAKNKGINCRSIIFPWKFLGKAISSNCSIPGLT
KLIVDTNSNRIIGGVIIGKHAGELLSQINLSIEMGCDIEDIALTIHPHPSLSESINISAQLFNGTATDMINKNII

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=34.1991341991342, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=26.9396551724138, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=24.3421052631579, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=24.3421052631579, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=24.3421052631579, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI22035672, Length=436, Percent_Identity=25, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=24.3421052631579, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=24.3421052631579, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=22.737819025522, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=21.6981132075472, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=51.380042462845, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=25.7510729613734, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=27.0562770562771, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789915, Length=426, Percent_Identity=23.943661971831, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=34.6578366445916, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71983429, Length=448, Percent_Identity=25.8928571428571, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=448, Percent_Identity=25.8928571428571, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=23.9754098360656, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=443, Percent_Identity=22.7990970654628, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=145, Percent_Identity=29.6551724137931, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=462, Percent_Identity=35.7142857142857, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=28.7211740041929, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=26.3736263736264, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358499, Length=456, Percent_Identity=34.4298245614035, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24640553, Length=496, Percent_Identity=25.4032258064516, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640551, Length=492, Percent_Identity=25.4065040650407, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640549, Length=492, Percent_Identity=25.4065040650407, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17737741, Length=488, Percent_Identity=23.5655737704918, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52249; Mature: 52249

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNINVNVVVIGGGPAGYSAAFRCSDLGFSTLIIEKYGILGGTCLNVGCIPSKSFLYLA
CCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHH
TLIKEIKEFSKYGIDLCANNKIDISKIMKWKDNIVLELSNGLKNMAEYRKIDILKGVAKF
HHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
FKKNQLKVILNNGKISYIKYDYAIIATGSKPITLSNFFSKDSRIWNSTHALNSDFIPKKL
HHCCCEEEEEECCEEEEEEEEEEEEEECCCCEEEHHHHCCCCHHCCCHHCCCCCCCCCEE
LIIGAGIIGLEMATIYSTLGSEVDIIDNSEKFFPLIDKDVSDVFLKYIQKYFSISLNTSI
EEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEEECCHHH
LSSISDKDGILVNTKINHNTYNSKMYHAILIAVGRTANTDCLDINIPGIKVDKYGFIQVD
HHHCCCCCCEEEEEEECCCCCCCHHEEEEEEEECCCCCCCEEEEECCCEEECCEEEEEEC
NQMRTNISNIFAIGDVVGQPMLAHKGMHEAHIAAEVIAGKKHFFDPKVIPCVAYCDPEIA
CHHHCCHHHHEEEHHHHCCCHHHHCCCCHHHHHHHHHHCCHHCCCCCCEEEEEECCCCEE
WTGIMEEEAKNKGINCRSIIFPWKFLGKAISSNCSIPGLTKLIVDTNSNRIIGGVIIGKH
EECCCHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEECCH
AGELLSQINLSIEMGCDIEDIALTIHPHPSLSESINISAQLFNGTATDMINKNII
HHHHHHHCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHCCCCC
>Mature Secondary Structure
MNKNINVNVVVIGGGPAGYSAAFRCSDLGFSTLIIEKYGILGGTCLNVGCIPSKSFLYLA
CCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHH
TLIKEIKEFSKYGIDLCANNKIDISKIMKWKDNIVLELSNGLKNMAEYRKIDILKGVAKF
HHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
FKKNQLKVILNNGKISYIKYDYAIIATGSKPITLSNFFSKDSRIWNSTHALNSDFIPKKL
HHCCCEEEEEECCEEEEEEEEEEEEEECCCCEEEHHHHCCCCHHCCCHHCCCCCCCCCEE
LIIGAGIIGLEMATIYSTLGSEVDIIDNSEKFFPLIDKDVSDVFLKYIQKYFSISLNTSI
EEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEEECCHHH
LSSISDKDGILVNTKINHNTYNSKMYHAILIAVGRTANTDCLDINIPGIKVDKYGFIQVD
HHHCCCCCCEEEEEEECCCCCCCHHEEEEEEEECCCCCCCEEEEECCCEEECCEEEEEEC
NQMRTNISNIFAIGDVVGQPMLAHKGMHEAHIAAEVIAGKKHFFDPKVIPCVAYCDPEIA
CHHHCCHHHHEEEHHHHCCCHHHHCCCCHHHHHHHHHHCCHHCCCCCCEEEEEECCCCEE
WTGIMEEEAKNKGINCRSIIFPWKFLGKAISSNCSIPGLTKLIVDTNSNRIIGGVIIGKH
EECCCHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEECCH
AGELLSQINLSIEMGCDIEDIALTIHPHPSLSESINISAQLFNGTATDMINKNII
HHHHHHHCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]