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The map label for this gene is prfA

Identifier: 116515052

GI number: 116515052

Start: 127420

End: 128493

Strand: Direct

Name: prfA

Synonym: BCc_111

Alternate gene names: 116515052

Gene position: 127420-128493 (Clockwise)

Preceding gene: 116515050

Following gene: 116515053

Centisome position: 30.6

GC content: 25.88

Gene sequence:

>1074_bases
ATGAAAAAATCAATAGTTAATAAGTTAAAATCTTTAAAAAAGAGATATATTCAATTAGAAAAATTATTGTCTAATAATTA
TTCAAAATTTGATAAGAGAGAATTTTCTAAACTTTCTAAAGAAAAAGCAAATTTATATGATTTATATAAAGAATTTATGT
CTTGGTTATCTATTAAATCTGAGATTAAAGATATTCAGTTATTATTAAATGATTTAGATCTTTCTACTTTAGCTCAGGAA
GAATTATTAATTGCTTTAAAAAGAAAGAAAGAAATAGAGAAGAAAATTAAAATGTTATTAATTCCTATTGATCCTTATGA
TAAAAATAGTTGTTTTATTGAAATTCGAGCAGCAACTGGTGGACATGAAGCAGCTATTTTTTCTGGAGATTTATGTAAAA
TGTATATGAAATACGCTGATTTCAAATCTTGGAAAGTAGATATTATTAATATGCATGAAGGTGAAAAAGGTGGATATAAA
GAAATTATTTTAAAAGTAATTGGAATTGGTGCTTGTGGAAGATTAAAATTTGAATCTGGTGGTCATAGAGTACAGCGTGT
TCCTCAAACAGAATCTCAAGGTCGTGTACATACTTCAGCATGTACAGTCGCAATTATGCCAGAAGTTCCTAAATCAAAAA
GAATTATTTTAAATACTTCTGATTTAAAAATTGATACTTTTCGTTCATCTGGAGCTGGTGGTCAACATGTTAATACAACA
GATTCAGCAGTGCGAATTACACATTTACCAACAGGTTGTGTAGTAGAATGTCAAGATGAAAGATCACAGCATAAAAATAA
AGAAAAAGCTTTATCAGTATTATCAGCAAAGATTTATTCTAAAATGAATGCAGAAAAAAAATTAAAAAATTCTTTAATGA
GAAAAAGTTTACTTGGAACTGGAGAAAGATCTGATAGAAATAGAACATATAATTTTTCACAAAATAGAGTTACTGATCAT
CGTATTAATTTAACTATATATCGTTTAAATGAAGTTTTGTTAGGAAAATTAGATTTATTAATTGAACCATTATTACAAGA
GCATCAAGCAGATTTATTATCAATAATAGATTAA

Upstream 100 bases:

>100_bases
TGTATATTTTTAATAGTTTATTTTTTTTTTTATTTTTTGATTATAATATAATTTAATGAATTTTAAATAATATTTTAAAG
TAATAAATAGGTTTATTTTT

Downstream 100 bases:

>100_bases
AATAATAGTGATATATAAAACAATGAATATTATAACTTGGTTATCATATGCTAGAAAAAAATTATCTAATAGTTTAACAG
CTAAATTAGATTCTGAAGTA

Product: hypothetical protein

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKSIVNKLKSLKKRYIQLEKLLSNNYSKFDKREFSKLSKEKANLYDLYKEFMSWLSIKSEIKDIQLLLNDLDLSTLAQE
ELLIALKRKKEIEKKIKMLLIPIDPYDKNSCFIEIRAATGGHEAAIFSGDLCKMYMKYADFKSWKVDIINMHEGEKGGYK
EIILKVIGIGACGRLKFESGGHRVQRVPQTESQGRVHTSACTVAIMPEVPKSKRIILNTSDLKIDTFRSSGAGGQHVNTT
DSAVRITHLPTGCVVECQDERSQHKNKEKALSVLSAKIYSKMNAEKKLKNSLMRKSLLGTGERSDRNRTYNFSQNRVTDH
RINLTIYRLNEVLLGKLDLLIEPLLQEHQADLLSIID

Sequences:

>Translated_357_residues
MKKSIVNKLKSLKKRYIQLEKLLSNNYSKFDKREFSKLSKEKANLYDLYKEFMSWLSIKSEIKDIQLLLNDLDLSTLAQE
ELLIALKRKKEIEKKIKMLLIPIDPYDKNSCFIEIRAATGGHEAAIFSGDLCKMYMKYADFKSWKVDIINMHEGEKGGYK
EIILKVIGIGACGRLKFESGGHRVQRVPQTESQGRVHTSACTVAIMPEVPKSKRIILNTSDLKIDTFRSSGAGGQHVNTT
DSAVRITHLPTGCVVECQDERSQHKNKEKALSVLSAKIYSKMNAEKKLKNSLMRKSLLGTGERSDRNRTYNFSQNRVTDH
RINLTIYRLNEVLLGKLDLLIEPLLQEHQADLLSIID
>Mature_357_residues
MKKSIVNKLKSLKKRYIQLEKLLSNNYSKFDKREFSKLSKEKANLYDLYKEFMSWLSIKSEIKDIQLLLNDLDLSTLAQE
ELLIALKRKKEIEKKIKMLLIPIDPYDKNSCFIEIRAATGGHEAAIFSGDLCKMYMKYADFKSWKVDIINMHEGEKGGYK
EIILKVIGIGACGRLKFESGGHRVQRVPQTESQGRVHTSACTVAIMPEVPKSKRIILNTSDLKIDTFRSSGAGGQHVNTT
DSAVRITHLPTGCVVECQDERSQHKNKEKALSVLSAKIYSKMNAEKKLKNSLMRKSLLGTGERSDRNRTYNFSQNRVTDH
RINLTIYRLNEVLLGKLDLLIEPLLQEHQADLLSIID

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=301, Percent_Identity=46.5116279069767, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI34577120, Length=359, Percent_Identity=37.883008356546, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.1139896373057, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=57.5842696629214, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI2367172, Length=245, Percent_Identity=42.0408163265306, Blast_Score=194, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=350, Percent_Identity=34, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=40.9523809523809, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI19921226, Length=261, Percent_Identity=40.9961685823755, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BUCCC (Q057W1)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802681.1
- ProteinModelPortal:   Q057W1
- SMR:   Q057W1
- STRING:   Q057W1
- EnsemblBacteria:   EBBUCT00000001469
- GeneID:   4440821
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_111
- eggNOG:   COG0216
- GeneTree:   EBGT00050000007801
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- BioCyc:   BAPH372461:BCC_111-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40764; Mature: 40764

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIVNKLKSLKKRYIQLEKLLSNNYSKFDKREFSKLSKEKANLYDLYKEFMSWLSIKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKDIQLLLNDLDLSTLAQEELLIALKRKKEIEKKIKMLLIPIDPYDKNSCFIEIRAATG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCC
GHEAAIFSGDLCKMYMKYADFKSWKVDIINMHEGEKGGYKEIILKVIGIGACGRLKFESG
CCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCC
GHRVQRVPQTESQGRVHTSACTVAIMPEVPKSKRIILNTSDLKIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCC
DSAVRITHLPTGCVVECQDERSQHKNKEKALSVLSAKIYSKMNAEKKLKNSLMRKSLLGT
CCEEEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
GERSDRNRTYNFSQNRVTDHRINLTIYRLNEVLLGKLDLLIEPLLQEHQADLLSIID
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKSIVNKLKSLKKRYIQLEKLLSNNYSKFDKREFSKLSKEKANLYDLYKEFMSWLSIKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKDIQLLLNDLDLSTLAQEELLIALKRKKEIEKKIKMLLIPIDPYDKNSCFIEIRAATG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCC
GHEAAIFSGDLCKMYMKYADFKSWKVDIINMHEGEKGGYKEIILKVIGIGACGRLKFESG
CCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCC
GHRVQRVPQTESQGRVHTSACTVAIMPEVPKSKRIILNTSDLKIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCC
DSAVRITHLPTGCVVECQDERSQHKNKEKALSVLSAKIYSKMNAEKKLKNSLMRKSLLGT
CCEEEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
GERSDRNRTYNFSQNRVTDHRINLTIYRLNEVLLGKLDLLIEPLLQEHQADLLSIID
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA