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The map label for this gene is pheT [H]

Identifier: 116515024

GI number: 116515024

Start: 89764

End: 92154

Strand: Direct

Name: pheT [H]

Synonym: BCc_083

Alternate gene names: 116515024

Gene position: 89764-92154 (Clockwise)

Preceding gene: 116515023

Following gene: 116515025

Centisome position: 21.56

GC content: 18.07

Gene sequence:

>2391_bases
ATGAAGTTTGGAGAAGAATGGTTACGTGAATGGATTAATCCATCTATTTCAGTTCCGGATTTATGTGAACAGTTAACAAA
TTTTGGTTGTGAAGTAGAATATTTTAAAAAAAAATATAAAACTATTTCTAGTGTAGTTATAGGTCAAATTATTTCAAAAG
AAAAATATTTTTTAAATAAAAAATTTTTAATATATAATATACTTATTAATAAAAATAAAAAAATTTATGTTGTATATTTT
AAAAAAAAATATTTATCTATAGGAAATAAAATACCTATTATTTTAGGTAAAAATTGTATTAAGAAAAATATAAAAAACTT
ATCATTAAATAATATAAATAATAATTTTTGTCATCATTTTGGTTCATATCATTTATTAGATATTCAAAAAAATAATCATA
AAATAATAGTTTTATCAAAATATGTTACCGTAGGTTTAAATTTTAATGATTTTTTTTATAAAAATGATTATTTAATGAAA
TTTTTTGTTCCTTTTAATCGTATTGATTTACGTTCTTTATGGGGTTTATCAAGAGAAATAGCGATATTAAATAAATTAAA
AATACCTAAATTAATACGTATAGATAATATACAAAATAAAAAAAAAAAACAAATAAATTGTAATATTAATATTGAAAATA
TTCCTATACAATATTTTTTTTGTGAATTATATAAAATAAATTTAGATTCAATTTTACCTTTTAAAATGCAAATAAGATTA
CTTCAATCAAATTTATTGACAGATAATATCATAGAAAATATTATTAATTATATTTTTATTGAAACTGGATATTGGTTTCA
TGTTTTTGATTTAGATCATATATCTAAAAATTTATATATTAAAAGTATTAAAAAAAAAACATATATTTTTGATACAAATA
AAAAAAAATTTTTTTTAAGAAAAGGAACAATAATATTAACAGATTTAAAAAATATTTTATCATTTGAAGATATGGAATAT
TCCTGTTATTCAAGGATTAGTAGTACTACTAAAAATTTATTTTTAGGTTCAATTTGTTTTTGTCCAAATTTTATTCAAAA
AAGAAATTTATTAATTCCATTTTTTTCTAGAAAAATAGAGTATTCTAAATATAATTTTTATCCTAAAATACAAAAGAATA
TATTTAAATATATACAAAAATTGATATTAAATATATGTGGTGGAAGATCTTCAAATATTAAAAAATATAATTTAATAAAA
AATTTTATTATTACTAATAAAATATTTTTTACTATTAGAAAATTAAATAAAATTTCTGGAATTATTTTTTTAAAAAAAGA
TATAATTACTATTTTAAAATCATGTTATTTTTCATATATAGAAAATAATGATTCATTTTATGTCACTCCTCCATATTGGC
GTCTTGATATTCAGATTGAAGAAGATGTAATTAGTGAAATTATTAGAATGTATGGTTACGAAAAAATTAAATCTTGTCCA
CCTCAAGAACATATCAATAGTAATATTTCTAATAAAAAATCTGAAGAAATGTCTTTATCTAGAATTAAATTTTTTCTTAT
TGATCAAGGATATAATGAAATTATATCATATAGTTTTGTAAATTTAAAAATGCAAAGATCTTTTATATCTAAAAATCATT
TTATAAAAATTCAAAATCCTATATCAAATGATATGTCAATTATGAGAACATCTCTATGGATTGGATTATTGCAATGTATT
TCATATCATCAAAATCGTCAATATGAATCAATTCGTGTTTTTGAATTTGGTTTGTGTTTTTATTTAAAAAATAAAAATAA
ATTTTCGATTATTCAAGATGAATATTTATCTGCAGCGATTAGTGGTTTTTCTAATCGTAGAGAGTGGTATTATAGAAAAA
GAAAATTTGATTTTTATGATTTAAAAGGTGATGTTGAATCAATTTTAAATATTTTAGGAAAATTTGATAATATTGAATTT
GTTTCTGAAAAATTTTTAGGATTATGTTTAGGAAAAAGTGCAGGAATTTATTTACACGGTGAATTAATTGGTTTTTTAGA
TAGTTCATTGCATCGTTTATTTAATTTAAAAAATTCAGTTATTTTATTTGAAATTAAATGGAAAAAAATTTGTAGTCATC
AAATTAAAAAAATTAAATCTATATCTACTCTTCCAATTAGTAAAAGAGATATTTCTATTATTATTTCTGATAAAATTATT
GTTAAAGACATAATTGATTTATGTAATAAAAATATTTATATTAAAAATTCTGAAATATATGTATATGATATTTATACAGG
ATCTAATGTTCCATCTGGAAAAAAAAGCATATCAATTTGTTTTCTTTTTAATAGTTTACAATCTATGTTAAAAGAATCTG
ATATTAATATAAATATTCTTCAATGTATTTCTGCATTAAAAGATAAATTTGGAGCTATTTTAAGAGAGTAA

Upstream 100 bases:

>100_bases
AAGAATAGCTATGTTAAAATATTCTGTATCTGACATTCGTTATTTTTTTGAAAATGATATACGATTTTTACAACAATTTT
CTTAACAACAGGAATATTTA

Downstream 100 bases:

>100_bases
ATTACTATGGTATTAACTAAAGCTGAAATTTCAGATTATTTATTTCATAAATTAGAATTAAGCAAACAAAATATTAAAGA
TTTAGTAGAAATTTTTTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MKFGEEWLREWINPSISVPDLCEQLTNFGCEVEYFKKKYKTISSVVIGQIISKEKYFLNKKFLIYNILINKNKKIYVVYF
KKKYLSIGNKIPIILGKNCIKKNIKNLSLNNINNNFCHHFGSYHLLDIQKNNHKIIVLSKYVTVGLNFNDFFYKNDYLMK
FFVPFNRIDLRSLWGLSREIAILNKLKIPKLIRIDNIQNKKKKQINCNINIENIPIQYFFCELYKINLDSILPFKMQIRL
LQSNLLTDNIIENIINYIFIETGYWFHVFDLDHISKNLYIKSIKKKTYIFDTNKKKFFLRKGTIILTDLKNILSFEDMEY
SCYSRISSTTKNLFLGSICFCPNFIQKRNLLIPFFSRKIEYSKYNFYPKIQKNIFKYIQKLILNICGGRSSNIKKYNLIK
NFIITNKIFFTIRKLNKISGIIFLKKDIITILKSCYFSYIENNDSFYVTPPYWRLDIQIEEDVISEIIRMYGYEKIKSCP
PQEHINSNISNKKSEEMSLSRIKFFLIDQGYNEIISYSFVNLKMQRSFISKNHFIKIQNPISNDMSIMRTSLWIGLLQCI
SYHQNRQYESIRVFEFGLCFYLKNKNKFSIIQDEYLSAAISGFSNRREWYYRKRKFDFYDLKGDVESILNILGKFDNIEF
VSEKFLGLCLGKSAGIYLHGELIGFLDSSLHRLFNLKNSVILFEIKWKKICSHQIKKIKSISTLPISKRDISIIISDKII
VKDIIDLCNKNIYIKNSEIYVYDIYTGSNVPSGKKSISICFLFNSLQSMLKESDININILQCISALKDKFGAILRE

Sequences:

>Translated_796_residues
MKFGEEWLREWINPSISVPDLCEQLTNFGCEVEYFKKKYKTISSVVIGQIISKEKYFLNKKFLIYNILINKNKKIYVVYF
KKKYLSIGNKIPIILGKNCIKKNIKNLSLNNINNNFCHHFGSYHLLDIQKNNHKIIVLSKYVTVGLNFNDFFYKNDYLMK
FFVPFNRIDLRSLWGLSREIAILNKLKIPKLIRIDNIQNKKKKQINCNINIENIPIQYFFCELYKINLDSILPFKMQIRL
LQSNLLTDNIIENIINYIFIETGYWFHVFDLDHISKNLYIKSIKKKTYIFDTNKKKFFLRKGTIILTDLKNILSFEDMEY
SCYSRISSTTKNLFLGSICFCPNFIQKRNLLIPFFSRKIEYSKYNFYPKIQKNIFKYIQKLILNICGGRSSNIKKYNLIK
NFIITNKIFFTIRKLNKISGIIFLKKDIITILKSCYFSYIENNDSFYVTPPYWRLDIQIEEDVISEIIRMYGYEKIKSCP
PQEHINSNISNKKSEEMSLSRIKFFLIDQGYNEIISYSFVNLKMQRSFISKNHFIKIQNPISNDMSIMRTSLWIGLLQCI
SYHQNRQYESIRVFEFGLCFYLKNKNKFSIIQDEYLSAAISGFSNRREWYYRKRKFDFYDLKGDVESILNILGKFDNIEF
VSEKFLGLCLGKSAGIYLHGELIGFLDSSLHRLFNLKNSVILFEIKWKKICSHQIKKIKSISTLPISKRDISIIISDKII
VKDIIDLCNKNIYIKNSEIYVYDIYTGSNVPSGKKSISICFLFNSLQSMLKESDININILQCISALKDKFGAILRE
>Mature_796_residues
MKFGEEWLREWINPSISVPDLCEQLTNFGCEVEYFKKKYKTISSVVIGQIISKEKYFLNKKFLIYNILINKNKKIYVVYF
KKKYLSIGNKIPIILGKNCIKKNIKNLSLNNINNNFCHHFGSYHLLDIQKNNHKIIVLSKYVTVGLNFNDFFYKNDYLMK
FFVPFNRIDLRSLWGLSREIAILNKLKIPKLIRIDNIQNKKKKQINCNINIENIPIQYFFCELYKINLDSILPFKMQIRL
LQSNLLTDNIIENIINYIFIETGYWFHVFDLDHISKNLYIKSIKKKTYIFDTNKKKFFLRKGTIILTDLKNILSFEDMEY
SCYSRISSTTKNLFLGSICFCPNFIQKRNLLIPFFSRKIEYSKYNFYPKIQKNIFKYIQKLILNICGGRSSNIKKYNLIK
NFIITNKIFFTIRKLNKISGIIFLKKDIITILKSCYFSYIENNDSFYVTPPYWRLDIQIEEDVISEIIRMYGYEKIKSCP
PQEHINSNISNKKSEEMSLSRIKFFLIDQGYNEIISYSFVNLKMQRSFISKNHFIKIQNPISNDMSIMRTSLWIGLLQCI
SYHQNRQYESIRVFEFGLCFYLKNKNKFSIIQDEYLSAAISGFSNRREWYYRKRKFDFYDLKGDVESILNILGKFDNIEF
VSEKFLGLCLGKSAGIYLHGELIGFLDSSLHRLFNLKNSVILFEIKWKKICSHQIKKIKSISTLPISKRDISIIISDKII
VKDIIDLCNKNIYIKNSEIYVYDIYTGSNVPSGKKSISICFLFNSLQSMLKESDININILQCISALKDKFGAILRE

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=813, Percent_Identity=27.060270602706, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 94234; Mature: 94234

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGEEWLREWINPSISVPDLCEQLTNFGCEVEYFKKKYKTISSVVIGQIISKEKYFLNK
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
KFLIYNILINKNKKIYVVYFKKKYLSIGNKIPIILGKNCIKKNIKNLSLNNINNNFCHHF
EEEEEEEEECCCCEEEEEEEEEEHHCCCCEEEEEECCHHHHHHHCCCEECCCCCHHHHHC
GSYHLLDIQKNNHKIIVLSKYVTVGLNFNDFFYKNDYLMKFFVPFNRIDLRSLWGLSREI
CCEEEEEEECCCCEEEEEEEEEEEECCCCCEEEECCEEEEEEECCCCCCHHHHHCCCHHH
AILNKLKIPKLIRIDNIQNKKKKQINCNINIENIPIQYFFCELYKINLDSILPFKMQIRL
HHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHH
LQSNLLTDNIIENIINYIFIETGYWFHVFDLDHISKNLYIKSIKKKTYIFDTNKKKFFLR
HHHHCCHHHHHHHHHHEEEEECCCEEEEEEHHHCCCCCEEEEECCEEEEEECCCCEEEEE
KGTIILTDLKNILSFEDMEYSCYSRISSTTKNLFLGSICFCPNFIQKRNLLIPFFSRKIE
CCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHCEEE
YSKYNFYPKIQKNIFKYIQKLILNICGGRSSNIKKYNLIKNFIITNKIFFTIRKLNKISG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCE
IIFLKKDIITILKSCYFSYIENNDSFYVTPPYWRLDIQIEEDVISEIIRMYGYEKIKSCP
EEEEHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECHHHHHHHHHHHCCHHHHCCC
PQEHINSNISNKKSEEMSLSRIKFFLIDQGYNEIISYSFVNLKMQRSFISKNHFIKIQNP
CHHHHCCCCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHEEEEEHHHHHCCCCEEEECCC
ISNDMSIMRTSLWIGLLQCISYHQNRQYESIRVFEFGLCFYLKNKNKFSIIQDEYLSAAI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCEEEEHHHHHHHHH
SGFSNRREWYYRKRKFDFYDLKGDVESILNILGKFDNIEFVSEKFLGLCLGKSAGIYLHG
HCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEH
ELIGFLDSSLHRLFNLKNSVILFEIKWKKICSHQIKKIKSISTLPISKRDISIIISDKII
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
VKDIIDLCNKNIYIKNSEIYVYDIYTGSNVPSGKKSISICFLFNSLQSMLKESDININIL
HHHHHHHHCCCEEEECCEEEEEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHH
QCISALKDKFGAILRE
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFGEEWLREWINPSISVPDLCEQLTNFGCEVEYFKKKYKTISSVVIGQIISKEKYFLNK
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
KFLIYNILINKNKKIYVVYFKKKYLSIGNKIPIILGKNCIKKNIKNLSLNNINNNFCHHF
EEEEEEEEECCCCEEEEEEEEEEHHCCCCEEEEEECCHHHHHHHCCCEECCCCCHHHHHC
GSYHLLDIQKNNHKIIVLSKYVTVGLNFNDFFYKNDYLMKFFVPFNRIDLRSLWGLSREI
CCEEEEEEECCCCEEEEEEEEEEEECCCCCEEEECCEEEEEEECCCCCCHHHHHCCCHHH
AILNKLKIPKLIRIDNIQNKKKKQINCNINIENIPIQYFFCELYKINLDSILPFKMQIRL
HHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHH
LQSNLLTDNIIENIINYIFIETGYWFHVFDLDHISKNLYIKSIKKKTYIFDTNKKKFFLR
HHHHCCHHHHHHHHHHEEEEECCCEEEEEEHHHCCCCCEEEEECCEEEEEECCCCEEEEE
KGTIILTDLKNILSFEDMEYSCYSRISSTTKNLFLGSICFCPNFIQKRNLLIPFFSRKIE
CCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHCEEE
YSKYNFYPKIQKNIFKYIQKLILNICGGRSSNIKKYNLIKNFIITNKIFFTIRKLNKISG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCE
IIFLKKDIITILKSCYFSYIENNDSFYVTPPYWRLDIQIEEDVISEIIRMYGYEKIKSCP
EEEEHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECHHHHHHHHHHHCCHHHHCCC
PQEHINSNISNKKSEEMSLSRIKFFLIDQGYNEIISYSFVNLKMQRSFISKNHFIKIQNP
CHHHHCCCCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHEEEEEHHHHHCCCCEEEECCC
ISNDMSIMRTSLWIGLLQCISYHQNRQYESIRVFEFGLCFYLKNKNKFSIIQDEYLSAAI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCEEEEHHHHHHHHH
SGFSNRREWYYRKRKFDFYDLKGDVESILNILGKFDNIEFVSEKFLGLCLGKSAGIYLHG
HCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEH
ELIGFLDSSLHRLFNLKNSVILFEIKWKKICSHQIKKIKSISTLPISKRDISIIISDKII
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
VKDIIDLCNKNIYIKNSEIYVYDIYTGSNVPSGKKSISICFLFNSLQSMLKESDININIL
HHHHHHHHCCCEEEECCEEEEEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHH
QCISALKDKFGAILRE
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]