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The map label for this gene is pheS [H]

Identifier: 116515023

GI number: 116515023

Start: 88747

End: 89748

Strand: Direct

Name: pheS [H]

Synonym: BCc_082

Alternate gene names: 116515023

Gene position: 88747-89748 (Clockwise)

Preceding gene: 116515022

Following gene: 116515024

Centisome position: 21.31

GC content: 20.06

Gene sequence:

>1002_bases
GTGAAAAAAAAAAAAGAAATATTCATTGTTTTTAAAAACATTTTTGTCTCTTTTAAAAAAGAGATTAAAAAAGTTAATAG
TATAATATTATTAAATAATTTTTGTTCTAAATTTTTAGGTAAAAAAAGTGTTTTATCTTTTTATTTTAAAAAAATAAAAA
AATTTGATATTATTAAACGTAAAAAATATAGTATTATTTTAAATGATTTTAAAAAAAAGATTAATAATATTATTTTAGAG
AAAAAAAAACAAATATATAATAATTCACAAAATCTTAAAAAAAAAAAAGTTTTTTTAGATTTTTCATTATCTGGTAGAAA
GACTAAAATAGGTAGTTTACATCCTATTACTATAGTTATTAATGAAATAAAAAAAATTTTTTATCATTATGGTTTTGAAA
TTTTTTACGGTCCTGAATTAGAACATATATATTATAATTTTGATTCTTTAAATGTTCCAATTAATCATCCATCTAGAAGT
AAAAATGATACTTTTTGGTTTAATCCTGATTATGTATTACGTACACAAACATCAAGTATGCAAATTAGAGTTTTAGAAAA
ATTTTCTTATCCTATTAAAGTTATTGTTCCTGGAAAAGTATATCGTCATGATTATGATCATACACACACTCCTATGTTTC
ATCAGATTGAAGGTTTAATAGTTAATAAATTAATTTCTTTTTCAAATTTAAAATGGATTTTGAAAGATTTTATTAAAAAA
ATTTTTAAAAAGAAAATTAAAGTACGTTTTCGAAATTCTTATTTTCCATTTACATGTCCATCTGCTGAAATGGATATTCA
AAACAAATTTGGAGAATGGATAGAAATATTAGGATGTGGTATGGTACTCCAATGTTTTAAAAATTTTGATATTGATTGTA
ATATCTATTCAGCATGTGCTTTTGGTATTGGTGTAGAAAGAATAGCTATGTTAAAATATTCTGTATCTGACATTCGTTAT
TTTTTTGAAAATGATATACGATTTTTACAACAATTTTCTTAA

Upstream 100 bases:

>100_bases
GAATTTTATAAATTTTATTAGTGGGAGTAACCAATCTCCCCTTTTAATTTTTTAAAATTAAAATATATTTTTATTTTTAT
ACATAAATATTGGTAAATTC

Downstream 100 bases:

>100_bases
CAACAGGAATATTTAATGAAGTTTGGAGAAGAATGGTTACGTGAATGGATTAATCCATCTATTTCAGTTCCGGATTTATG
TGAACAGTTAACAAATTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKKKEIFIVFKNIFVSFKKEIKKVNSIILLNNFCSKFLGKKSVLSFYFKKIKKFDIIKRKKYSIILNDFKKKINNIILE
KKKQIYNNSQNLKKKKVFLDFSLSGRKTKIGSLHPITIVINEIKKIFYHYGFEIFYGPELEHIYYNFDSLNVPINHPSRS
KNDTFWFNPDYVLRTQTSSMQIRVLEKFSYPIKVIVPGKVYRHDYDHTHTPMFHQIEGLIVNKLISFSNLKWILKDFIKK
IFKKKIKVRFRNSYFPFTCPSAEMDIQNKFGEWIEILGCGMVLQCFKNFDIDCNIYSACAFGIGVERIAMLKYSVSDIRY
FFENDIRFLQQFS

Sequences:

>Translated_333_residues
MKKKKEIFIVFKNIFVSFKKEIKKVNSIILLNNFCSKFLGKKSVLSFYFKKIKKFDIIKRKKYSIILNDFKKKINNIILE
KKKQIYNNSQNLKKKKVFLDFSLSGRKTKIGSLHPITIVINEIKKIFYHYGFEIFYGPELEHIYYNFDSLNVPINHPSRS
KNDTFWFNPDYVLRTQTSSMQIRVLEKFSYPIKVIVPGKVYRHDYDHTHTPMFHQIEGLIVNKLISFSNLKWILKDFIKK
IFKKKIKVRFRNSYFPFTCPSAEMDIQNKFGEWIEILGCGMVLQCFKNFDIDCNIYSACAFGIGVERIAMLKYSVSDIRY
FFENDIRFLQQFS
>Mature_333_residues
MKKKKEIFIVFKNIFVSFKKEIKKVNSIILLNNFCSKFLGKKSVLSFYFKKIKKFDIIKRKKYSIILNDFKKKINNIILE
KKKQIYNNSQNLKKKKVFLDFSLSGRKTKIGSLHPITIVINEIKKIFYHYGFEIFYGPELEHIYYNFDSLNVPINHPSRS
KNDTFWFNPDYVLRTQTSSMQIRVLEKFSYPIKVIVPGKVYRHDYDHTHTPMFHQIEGLIVNKLISFSNLKWILKDFIKK
IFKKKIKVRFRNSYFPFTCPSAEMDIQNKFGEWIEILGCGMVLQCFKNFDIDCNIYSACAFGIGVERIAMLKYSVSDIRY
FFENDIRFLQQFS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=275, Percent_Identity=32.7272727272727, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4758340, Length=257, Percent_Identity=28.0155642023346, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1788007, Length=323, Percent_Identity=44.2724458204334, Blast_Score=333, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI32566635, Length=239, Percent_Identity=34.3096234309623, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=29.4339622641509, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=29.4339622641509, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325304, Length=316, Percent_Identity=28.4810126582279, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137424, Length=297, Percent_Identity=30.3030303030303, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39769; Mature: 39769

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKEIFIVFKNIFVSFKKEIKKVNSIILLNNFCSKFLGKKSVLSFYFKKIKKFDIIKR
CCCCCEEEEEHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KKYSIILNDFKKKINNIILEKKKQIYNNSQNLKKKKVFLDFSLSGRKTKIGSLHPITIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEECCCCCHHHHHH
NEIKKIFYHYGFEIFYGPELEHIYYNFDSLNVPINHPSRSKNDTFWFNPDYVLRTQTSSM
HHHHHHHHHCCEEEEECCCEEEEEEECCEEECCCCCCCCCCCCCEEECCCEEEEECCCCE
QIRVLEKFSYPIKVIVPGKVYRHDYDHTHTPMFHQIEGLIVNKLISFSNLKWILKDFIKK
EEEEEECCCCCEEEEECCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKKKIKVRFRNSYFPFTCPSAEMDIQNKFGEWIEILGCGMVLQCFKNFDIDCNIYSACA
HHHHHHHEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FGIGVERIAMLKYSVSDIRYFFENDIRFLQQFS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKKEIFIVFKNIFVSFKKEIKKVNSIILLNNFCSKFLGKKSVLSFYFKKIKKFDIIKR
CCCCCEEEEEHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KKYSIILNDFKKKINNIILEKKKQIYNNSQNLKKKKVFLDFSLSGRKTKIGSLHPITIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEECCCCCHHHHHH
NEIKKIFYHYGFEIFYGPELEHIYYNFDSLNVPINHPSRSKNDTFWFNPDYVLRTQTSSM
HHHHHHHHHCCEEEEECCCEEEEEEECCEEECCCCCCCCCCCCCEEECCCEEEEECCCCE
QIRVLEKFSYPIKVIVPGKVYRHDYDHTHTPMFHQIEGLIVNKLISFSNLKWILKDFIKK
EEEEEECCCCCEEEEECCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKKKIKVRFRNSYFPFTCPSAEMDIQNKFGEWIEILGCGMVLQCFKNFDIDCNIYSACA
HHHHHHHEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FGIGVERIAMLKYSVSDIRYFFENDIRFLQQFS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]