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The map label for this gene is metG [H]

Identifier: 116515014

GI number: 116515014

Start: 78929

End: 80572

Strand: Direct

Name: metG [H]

Synonym: BCc_072

Alternate gene names: 116515014

Gene position: 78929-80572 (Clockwise)

Preceding gene: 116515013

Following gene: 116515016

Centisome position: 18.96

GC content: 19.53

Gene sequence:

>1644_bases
ATGAATCATATTTCAGATAAAATATTAGTTACTTGTGCTTTTCCATATGCAAATGGTGATATACATTTAGGACATTTATT
AGAACAAATACAAGCTGATATTTGGGTACGGTATCATCGTATGAAACATCGTTCTGTAGTCTTTATTTGTTCTGATGATA
CACATGGAACAGCTATTATGTTAAAATCAAAAAATATGAATATTAGTTCTGAAAAACTTATTCATGATATAGGTATTAAA
CATAAAAGTGATTTTTTAAAATTTTCTATTATTCATGATTATTATTCATCTACACATAGTCAAGTAAATAAAGATTTATG
TTGTAATATATTTAAAATTTTAAAAAAAAAAAAATTTATTAAAGAAAAAATAATTTCTCAATTGTATGATAAAAAATTAA
ATATGTTTTTACCTGATAGATTTATTAAAGGTATTTGTCCTAAATGTAAATCAAATAATCAGTATGGTGATAACTGTGAT
TATTGTGGAGCTTTTTATAATGCTATAGAATTATTAAATCCTATTTCTGAATTATCAAAAACTAAACCAATTATAAAACA
ATCAAATCATCTTTTTTTTAAATTATCATATTTTAAAAAATTTTTAAAAACTTGGATAAATTCAGGTATTACACAAAAGT
CTATATTAAATAAATTAAATGATTTATTATCTAAAAAATTATGTTCTTGGAATATATCAAGGGATAAACCTTATTTTGGT
TTTGAGATTCCAGGATATATTGATAAATTTTTTTATGTTTGGATGGATGCGCCTATTGGTTATATTAGTTGTATTCAAGA
ATTTTGTAATATTAATAAAACAGTAAATTTTAATGATTTTTGGTCTAAACATTCTAAATATCAATTATTTCATTTTATAG
GTAAAGATATTATTTATTTTCATTGTTTATTTTGGCCTGCTATTCTTGAAGGATGTGAATATAGAAAACCTACTAATATT
TTTGTACACGGATATGTAACTTTAAATAAATTAAAATTATCTAAATCTAAAGGTTCAATAATAACTGCTAATCAATGGTT
ATCGTATTTTGATTCAGATTCTTTAAGATATTATTTTGCTTCTAAATTATCAAATAATATTGAAGATATAGAAATTAATT
TATATGATTACGCATATAGAATTAATTCAGATATTGTAAATAATGTAATTAATTTAGCATCCCGAAGTTATTTTTTTCTA
AAAAAATATTTTTTTAGTATTTTATCTAATAATTTGTTAAAAGAAAAATTGTATAAGAAATTTGTTAGTTCTATTAAAGA
TATTGAAAATTTTTTTGAAAAAAGAAAATTTTCTTTAATTATACAAAAAATTAGATTTTTATCTCAAAAAGCTAATAAAT
ATTTTAATGATCAAAAACCATGGGTGTTAATAAAAAATATTATTTATAAAAGTAAAGTTCATGATATTTGTTCTTTATCA
ATTAATTTATTTAGAATTTTAATGACTTTTCTTAAACCTATTATGCCTACATTAGTTAAAAAATCTGAATTTTTTTTAAA
TAAAAAATTATTATGGGAAAAAATTAAAATTCCACTAACTAATCATAAACTGAATAATTTTTCTTGTTTTTATAAACGTA
TTAATATTAATGATATAGATAAATTTTTAAAGAAAGTAATATAA

Upstream 100 bases:

>100_bases
TATTTTTATAACAAAATTTATATTACAGTTTTCATAAATAAAAATTTGGTTATTTAAATATAATTTATTAATTTAACTAT
TTATTTTTTTTGGTTATTAT

Downstream 100 bases:

>100_bases
TTAATTTATTTTGTTTATCCATTTAATTGATATATATTTTTTTTTTTCTTTTTTTTTTTCTTTTTCTATAAATATACCAA
TACATGATATTAATTTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNHISDKILVTCAFPYANGDIHLGHLLEQIQADIWVRYHRMKHRSVVFICSDDTHGTAIMLKSKNMNISSEKLIHDIGIK
HKSDFLKFSIIHDYYSSTHSQVNKDLCCNIFKILKKKKFIKEKIISQLYDKKLNMFLPDRFIKGICPKCKSNNQYGDNCD
YCGAFYNAIELLNPISELSKTKPIIKQSNHLFFKLSYFKKFLKTWINSGITQKSILNKLNDLLSKKLCSWNISRDKPYFG
FEIPGYIDKFFYVWMDAPIGYISCIQEFCNINKTVNFNDFWSKHSKYQLFHFIGKDIIYFHCLFWPAILEGCEYRKPTNI
FVHGYVTLNKLKLSKSKGSIITANQWLSYFDSDSLRYYFASKLSNNIEDIEINLYDYAYRINSDIVNNVINLASRSYFFL
KKYFFSILSNNLLKEKLYKKFVSSIKDIENFFEKRKFSLIIQKIRFLSQKANKYFNDQKPWVLIKNIIYKSKVHDICSLS
INLFRILMTFLKPIMPTLVKKSEFFLNKKLLWEKIKIPLTNHKLNNFSCFYKRININDIDKFLKKVI

Sequences:

>Translated_547_residues
MNHISDKILVTCAFPYANGDIHLGHLLEQIQADIWVRYHRMKHRSVVFICSDDTHGTAIMLKSKNMNISSEKLIHDIGIK
HKSDFLKFSIIHDYYSSTHSQVNKDLCCNIFKILKKKKFIKEKIISQLYDKKLNMFLPDRFIKGICPKCKSNNQYGDNCD
YCGAFYNAIELLNPISELSKTKPIIKQSNHLFFKLSYFKKFLKTWINSGITQKSILNKLNDLLSKKLCSWNISRDKPYFG
FEIPGYIDKFFYVWMDAPIGYISCIQEFCNINKTVNFNDFWSKHSKYQLFHFIGKDIIYFHCLFWPAILEGCEYRKPTNI
FVHGYVTLNKLKLSKSKGSIITANQWLSYFDSDSLRYYFASKLSNNIEDIEINLYDYAYRINSDIVNNVINLASRSYFFL
KKYFFSILSNNLLKEKLYKKFVSSIKDIENFFEKRKFSLIIQKIRFLSQKANKYFNDQKPWVLIKNIIYKSKVHDICSLS
INLFRILMTFLKPIMPTLVKKSEFFLNKKLLWEKIKIPLTNHKLNNFSCFYKRININDIDKFLKKVI
>Mature_547_residues
MNHISDKILVTCAFPYANGDIHLGHLLEQIQADIWVRYHRMKHRSVVFICSDDTHGTAIMLKSKNMNISSEKLIHDIGIK
HKSDFLKFSIIHDYYSSTHSQVNKDLCCNIFKILKKKKFIKEKIISQLYDKKLNMFLPDRFIKGICPKCKSNNQYGDNCD
YCGAFYNAIELLNPISELSKTKPIIKQSNHLFFKLSYFKKFLKTWINSGITQKSILNKLNDLLSKKLCSWNISRDKPYFG
FEIPGYIDKFFYVWMDAPIGYISCIQEFCNINKTVNFNDFWSKHSKYQLFHFIGKDIIYFHCLFWPAILEGCEYRKPTNI
FVHGYVTLNKLKLSKSKGSIITANQWLSYFDSDSLRYYFASKLSNNIEDIEINLYDYAYRINSDIVNNVINLASRSYFFL
KKYFFSILSNNLLKEKLYKKFVSSIKDIENFFEKRKFSLIIQKIRFLSQKANKYFNDQKPWVLIKNIIYKSKVHDICSLS
INLFRILMTFLKPIMPTLVKKSEFFLNKKLLWEKIKIPLTNHKLNNFSCFYKRININDIDKFLKKVI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=564, Percent_Identity=26.4184397163121, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI24308436, Length=515, Percent_Identity=21.9417475728155, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1788432, Length=545, Percent_Identity=49.5412844036697, Blast_Score=625, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17540794, Length=573, Percent_Identity=25.3054101221641, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI212645096, Length=556, Percent_Identity=22.841726618705, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=380, Percent_Identity=25.5263157894737, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=572, Percent_Identity=27.0979020979021, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=23.1192660550459, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=28.2918149466192, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI21358483, Length=538, Percent_Identity=22.4907063197026, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 64879; Mature: 64879

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHISDKILVTCAFPYANGDIHLGHLLEQIQADIWVRYHRMKHRSVVFICSDDTHGTAIM
CCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKSKNMNISSEKLIHDIGIKHKSDFLKFSIIHDYYSSTHSQVNKDLCCNIFKILKKKKFI
EECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKIISQLYDKKLNMFLPDRFIKGICPKCKSNNQYGDNCDYCGAFYNAIELLNPISELSK
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TKPIIKQSNHLFFKLSYFKKFLKTWINSGITQKSILNKLNDLLSKKLCSWNISRDKPYFG
CCCHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FEIPGYIDKFFYVWMDAPIGYISCIQEFCNINKTVNFNDFWSKHSKYQLFHFIGKDIIYF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHH
HCLFWPAILEGCEYRKPTNIFVHGYVTLNKLKLSKSKGSIITANQWLSYFDSDSLRYYFA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHCCCCHHHHHH
SKLSNNIEDIEINLYDYAYRINSDIVNNVINLASRSYFFLKKYFFSILSNNLLKEKLYKK
HHHCCCCCEEEEEEEHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVSSIKDIENFFEKRKFSLIIQKIRFLSQKANKYFNDQKPWVLIKNIIYKSKVHDICSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
INLFRILMTFLKPIMPTLVKKSEFFLNKKLLWEKIKIPLTNHKLNNFSCFYKRININDID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHH
KFLKKVI
HHHHHHC
>Mature Secondary Structure
MNHISDKILVTCAFPYANGDIHLGHLLEQIQADIWVRYHRMKHRSVVFICSDDTHGTAIM
CCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKSKNMNISSEKLIHDIGIKHKSDFLKFSIIHDYYSSTHSQVNKDLCCNIFKILKKKKFI
EECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKIISQLYDKKLNMFLPDRFIKGICPKCKSNNQYGDNCDYCGAFYNAIELLNPISELSK
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TKPIIKQSNHLFFKLSYFKKFLKTWINSGITQKSILNKLNDLLSKKLCSWNISRDKPYFG
CCCHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FEIPGYIDKFFYVWMDAPIGYISCIQEFCNINKTVNFNDFWSKHSKYQLFHFIGKDIIYF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHH
HCLFWPAILEGCEYRKPTNIFVHGYVTLNKLKLSKSKGSIITANQWLSYFDSDSLRYYFA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHCCCCHHHHHH
SKLSNNIEDIEINLYDYAYRINSDIVNNVINLASRSYFFLKKYFFSILSNNLLKEKLYKK
HHHCCCCCEEEEEEEHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVSSIKDIENFFEKRKFSLIIQKIRFLSQKANKYFNDQKPWVLIKNIIYKSKVHDICSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
INLFRILMTFLKPIMPTLVKKSEFFLNKKLLWEKIKIPLTNHKLNNFSCFYKRININDID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHH
KFLKKVI
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA