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The map label for this gene is trmE

Identifier: 116493570

GI number: 116493570

Start: 1828780

End: 1830174

Strand: Reverse

Name: trmE

Synonym: PEPE_1844

Alternate gene names: 116493570

Gene position: 1830174-1828780 (Counterclockwise)

Preceding gene: 116493571

Following gene: 116493569

Centisome position: 99.88

GC content: 37.35

Gene sequence:

>1395_bases
ATGCAAACTGTTTCAACTGAATTTGACACAATTGCAGCAATTTCAACTCCACCTGGGGAAGGCGGAATTTCAATTATTCG
AATTAGCGGAGTGGATGCACTTAAAACTGCCAGCCAGATTTATCGTGGCAAAGATTTGAATAAGGTGAATAGCCATACCA
TTAATTATGGTCATATTATTGATCCAGAAAATGGAAATGAAGTAGATGAAGTAATGGTGTCTGTAATGCGTGCGCCACAT
ACATACACCAAGGAAGATATCGTCGAAATTAATTGTCATGGTGGGATTGTCGCAACTAACCGAATTTTACAGATTATTTT
AGGGTTAGATGCGAGATTGGCTAAACCGGGTGAATTTACTGAACGAGCTTTTTTGAACGGACGAATCGATTTGTCTCAAG
CAGAAGCCGTAATGGATTTAATTCGTGCTAAAACTGATCAATCGATGAAAGTGGCGCTTGATCAGTTAGATGGAAATTTA
TCCCATTTAATTACTAATTTACGTCAAAATATCTTAGATGTTTTAGCACAAGTTGAAGTTAACATTGATTATCCAGAATA
TGACGATGTCGAAACAATGACAGCTCGGTTACTTAAAGAAAAAGCAATTGAAGTTAAAGCGAAGATTCAACAATTACTTT
CAACTGCTAAACAAGGTAAAGTGCTCCGTGATGGGCTGGCTACCGCCATTATTGGGCATCCCAATGTCGGTAAATCTAGT
ATTCTAAATCATCTTTTACATGAAGATAAGGCCATCGTCACGGATGTTGCTGGTACAACTCGTGATGTAATTGAAGAATA
TGTTAATGTTCAAGGGGTCCCACTTAAGTTGGTTGACACAGCTGGTATTCATGAAACAGAAGATAAAGTTGAAAAAATTG
GAGTGGATCGAAGCCGCAAAGCTCTTTCACAAGCCGATTTAGTTATCTTGGTTTTAGATAGTAGTGTCCCACTGCGCGAT
GAGGATCGGGAGCTGCTAAGAGAAACCAATCATATGCAGCGGATCGTCGTGCTTAATAAATCAGATTTAGAAGTAAAAAT
TAACTTAAATGAACTTCAAGAATATGTAGATGATAAAGAAATTATCAAATCATCAGCCGTTTCACCTTTAGGAACCAAAG
ACCTTGAGGATCGTATTGCAGCAATGTTCTTTGCTGGAAGCATTGAAAATACCTCTAATAATATTATGGTAACTAACGCT
CGTCATATTGGATTGTTGAAGCAAGCTGATACAGCTTTAGATGCCGTTCTTGAAGGGATTGAAACAGGAATGCCAGTTGA
TTTAGTTCAAATCGATATGACACGTACTTGGGATTTATTGGGTGAAATTACTGGTGATAGTTACCAAGACGAATTATTAG
ATCAGTTATTTAGTCAATTTTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
ATGAAGTAGTGAAAGTGCTTTGTCCACCCTATATCCAAGTGGCTAACTGGATTATGGGTGGAACTGCGCTTTTTTTATTG
GAAAAAGAAAGGATAACATT

Downstream 100 bases:

>100_bases
ATTTGGAGTTGAAAAATATGTCTGAAGTAAAACAATATGAAGGTCGCGAGTATGACGTAATTGTAGTAGGAGCTGGACAT
GCTGGCTCGGAAGCGGCGCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MQTVSTEFDTIAAISTPPGEGGISIIRISGVDALKTASQIYRGKDLNKVNSHTINYGHIIDPENGNEVDEVMVSVMRAPH
TYTKEDIVEINCHGGIVATNRILQIILGLDARLAKPGEFTERAFLNGRIDLSQAEAVMDLIRAKTDQSMKVALDQLDGNL
SHLITNLRQNILDVLAQVEVNIDYPEYDDVETMTARLLKEKAIEVKAKIQQLLSTAKQGKVLRDGLATAIIGHPNVGKSS
ILNHLLHEDKAIVTDVAGTTRDVIEEYVNVQGVPLKLVDTAGIHETEDKVEKIGVDRSRKALSQADLVILVLDSSVPLRD
EDRELLRETNHMQRIVVLNKSDLEVKINLNELQEYVDDKEIIKSSAVSPLGTKDLEDRIAAMFFAGSIENTSNNIMVTNA
RHIGLLKQADTALDAVLEGIETGMPVDLVQIDMTRTWDLLGEITGDSYQDELLDQLFSQFCLGK

Sequences:

>Translated_464_residues
MQTVSTEFDTIAAISTPPGEGGISIIRISGVDALKTASQIYRGKDLNKVNSHTINYGHIIDPENGNEVDEVMVSVMRAPH
TYTKEDIVEINCHGGIVATNRILQIILGLDARLAKPGEFTERAFLNGRIDLSQAEAVMDLIRAKTDQSMKVALDQLDGNL
SHLITNLRQNILDVLAQVEVNIDYPEYDDVETMTARLLKEKAIEVKAKIQQLLSTAKQGKVLRDGLATAIIGHPNVGKSS
ILNHLLHEDKAIVTDVAGTTRDVIEEYVNVQGVPLKLVDTAGIHETEDKVEKIGVDRSRKALSQADLVILVLDSSVPLRD
EDRELLRETNHMQRIVVLNKSDLEVKINLNELQEYVDDKEIIKSSAVSPLGTKDLEDRIAAMFFAGSIENTSNNIMVTNA
RHIGLLKQADTALDAVLEGIETGMPVDLVQIDMTRTWDLLGEITGDSYQDELLDQLFSQFCLGK
>Mature_464_residues
MQTVSTEFDTIAAISTPPGEGGISIIRISGVDALKTASQIYRGKDLNKVNSHTINYGHIIDPENGNEVDEVMVSVMRAPH
TYTKEDIVEINCHGGIVATNRILQIILGLDARLAKPGEFTERAFLNGRIDLSQAEAVMDLIRAKTDQSMKVALDQLDGNL
SHLITNLRQNILDVLAQVEVNIDYPEYDDVETMTARLLKEKAIEVKAKIQQLLSTAKQGKVLRDGLATAIIGHPNVGKSS
ILNHLLHEDKAIVTDVAGTTRDVIEEYVNVQGVPLKLVDTAGIHETEDKVEKIGVDRSRKALSQADLVILVLDSSVPLRD
EDRELLRETNHMQRIVVLNKSDLEVKINLNELQEYVDDKEIIKSSAVSPLGTKDLEDRIAAMFFAGSIENTSNNIMVTNA
RHIGLLKQADTALDAVLEGIETGMPVDLVQIDMTRTWDLLGEITGDSYQDELLDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=31.9148936170213, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=31.3725490196078, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=30.060120240481, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=34.6153846153846, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI87082120, Length=135, Percent_Identity=37.037037037037, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=27.0967741935484, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=30.7070707070707, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=30.5154639175258, Blast_Score=197, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PEDPA (Q03D59)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805305.1
- ProteinModelPortal:   Q03D59
- SMR:   Q03D59
- STRING:   Q03D59
- GeneID:   4417373
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1844
- NMPDR:   fig|278197.10.peg.1634
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q03D59
- ProtClustDB:   PRK05291
- BioCyc:   PPEN278197:PEPE_1844-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51160; Mature: 51160

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 89-89 BINDING 128-128 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVSTEFDTIAAISTPPGEGGISIIRISGVDALKTASQIYRGKDLNKVNSHTINYGHII
CCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEECCCEEE
DPENGNEVDEVMVSVMRAPHTYTKEDIVEINCHGGIVATNRILQIILGLDARLAKPGEFT
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCHHCCCCCCHH
ERAFLNGRIDLSQAEAVMDLIRAKTDQSMKVALDQLDGNLSHLITNLRQNILDVLAQVEV
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
NIDYPEYDDVETMTARLLKEKAIEVKAKIQQLLSTAKQGKVLRDGLATAIIGHPNVGKSS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHEEECCCCCCHHH
ILNHLLHEDKAIVTDVAGTTRDVIEEYVNVQGVPLKLVDTAGIHETEDKVEKIGVDRSRK
HHHHHHHCCCCEEHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHH
ALSQADLVILVLDSSVPLRDEDRELLRETNHMQRIVVLNKSDLEVKINLNELQEYVDDKE
HHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHHHHHCHHH
IIKSSAVSPLGTKDLEDRIAAMFFAGSIENTSNNIMVTNARHIGLLKQADTALDAVLEGI
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
ETGMPVDLVQIDMTRTWDLLGEITGDSYQDELLDQLFSQFCLGK
HCCCCEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTVSTEFDTIAAISTPPGEGGISIIRISGVDALKTASQIYRGKDLNKVNSHTINYGHII
CCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEECCCEEE
DPENGNEVDEVMVSVMRAPHTYTKEDIVEINCHGGIVATNRILQIILGLDARLAKPGEFT
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCHHCCCCCCHH
ERAFLNGRIDLSQAEAVMDLIRAKTDQSMKVALDQLDGNLSHLITNLRQNILDVLAQVEV
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
NIDYPEYDDVETMTARLLKEKAIEVKAKIQQLLSTAKQGKVLRDGLATAIIGHPNVGKSS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHEEECCCCCCHHH
ILNHLLHEDKAIVTDVAGTTRDVIEEYVNVQGVPLKLVDTAGIHETEDKVEKIGVDRSRK
HHHHHHHCCCCEEHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHH
ALSQADLVILVLDSSVPLRDEDRELLRETNHMQRIVVLNKSDLEVKINLNELQEYVDDKE
HHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHHHHHCHHH
IIKSSAVSPLGTKDLEDRIAAMFFAGSIENTSNNIMVTNARHIGLLKQADTALDAVLEGI
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
ETGMPVDLVQIDMTRTWDLLGEITGDSYQDELLDQLFSQFCLGK
HCCCCEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA