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The map label for this gene is lplA [H]

Identifier: 116493502

GI number: 116493502

Start: 1748718

End: 1749704

Strand: Reverse

Name: lplA [H]

Synonym: PEPE_1774

Alternate gene names: 116493502

Gene position: 1749704-1748718 (Counterclockwise)

Preceding gene: 116493503

Following gene: 116493501

Centisome position: 95.49

GC content: 40.02

Gene sequence:

>987_bases
GTGCGATACGTCACATACATGGGAACAGATGCGTACACTAATATTGCAACGGACACATGGCTACTAAATAATTTAAATGC
CAAGGAACCAGTTTTTGCACTTTGGCAGAATAATAATGCTATTATTGTTGGTCAGAATCAGAATACTTTTGGGGAAGTTA
ACCAAGATTTTGTTGATGAACACCAAATTGAGGTTGTCCGGCGAGTGACGGGCGGTGGCGCAGTTTACCATGACTTAGGT
AATCTGAACTTCACCTTATTTGTACCAATTGAATCAGCAGCAAATGTTGATTTTAAAAAGTTTTCTGAGCCCGTATTAAG
AGCTTTACATACTTTAGGGATTGATGCCGAGGCTTCCGGTCGAAATGATCTGTTAGTAAATGGTAAAAAAATCTCTGGTG
TAGCCCAGCGCTATGCCAAAGGATACTTAATGCATCATGGAACATTGCTATTTGATAGTAATGTAGATACCATGGTTCGT
TCCTTAAATGTAGCGGATGAAAAATTCATTTCAAAAGCTGCTAAATCGGTCCGAAGTCGAGTGGGAATGATCAAAGATGC
AGCTCCAAAAGGGTTAACCCTTCAAGATTTCTGGGATGCGGTTCAGTACGAATTAACCGATCACGGGAAAGACCAAGAGA
TTGTGTTAACTCCGCAACAAAAACAGGAGATTATCCAACTAACGGCTGATAAGTTTTCAACTTGGGACTGGAATTATGGT
CGTTCACCAGAATTTAACTTTAAGAACCATGCTAAATATGACGGTGGAATTATCGATGTTCAAGTCCAAGCTGAGGGTGG
AACCATACGTGATATCAATTTCACAGGCGATTTCCTTGGTTTACGCGATTGGCGCGAAATCAAAGACCAATTTATTGGGT
TACCATTTGAAACTGTAGAGATTAAGCCCGTCTTTGAACGTAACCATGAAGGCCGGTACTTCGGAAACATTACGGATGAT
GAATTAATTAAATTATTTGAAGCTTAG

Upstream 100 bases:

>100_bases
CATTTTAAACCCAAAAAATAGCCCTAAAACGGTTCAAATGTGCTATCATAAAGATGTACTTAGAAAGCGCTTCCATTAGC
GTGATAAGGGGGTTTAGATT

Downstream 100 bases:

>100_bases
GAGGTTTAGTTATGGCTGTAAAAGATGACAATAAACAAGTGTTAGATTTCAATCTTCAAATGGAAAAACAAACAGACAGT
TTTCCAATGCTACAAGTTCT

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRYVTYMGTDAYTNIATDTWLLNNLNAKEPVFALWQNNNAIIVGQNQNTFGEVNQDFVDEHQIEVVRRVTGGGAVYHDLG
NLNFTLFVPIESAANVDFKKFSEPVLRALHTLGIDAEASGRNDLLVNGKKISGVAQRYAKGYLMHHGTLLFDSNVDTMVR
SLNVADEKFISKAAKSVRSRVGMIKDAAPKGLTLQDFWDAVQYELTDHGKDQEIVLTPQQKQEIIQLTADKFSTWDWNYG
RSPEFNFKNHAKYDGGIIDVQVQAEGGTIRDINFTGDFLGLRDWREIKDQFIGLPFETVEIKPVFERNHEGRYFGNITDD
ELIKLFEA

Sequences:

>Translated_328_residues
MRYVTYMGTDAYTNIATDTWLLNNLNAKEPVFALWQNNNAIIVGQNQNTFGEVNQDFVDEHQIEVVRRVTGGGAVYHDLG
NLNFTLFVPIESAANVDFKKFSEPVLRALHTLGIDAEASGRNDLLVNGKKISGVAQRYAKGYLMHHGTLLFDSNVDTMVR
SLNVADEKFISKAAKSVRSRVGMIKDAAPKGLTLQDFWDAVQYELTDHGKDQEIVLTPQQKQEIIQLTADKFSTWDWNYG
RSPEFNFKNHAKYDGGIIDVQVQAEGGTIRDINFTGDFLGLRDWREIKDQFIGLPFETVEIKPVFERNHEGRYFGNITDD
ELIKLFEA
>Mature_328_residues
MRYVTYMGTDAYTNIATDTWLLNNLNAKEPVFALWQNNNAIIVGQNQNTFGEVNQDFVDEHQIEVVRRVTGGGAVYHDLG
NLNFTLFVPIESAANVDFKKFSEPVLRALHTLGIDAEASGRNDLLVNGKKISGVAQRYAKGYLMHHGTLLFDSNVDTMVR
SLNVADEKFISKAAKSVRSRVGMIKDAAPKGLTLQDFWDAVQYELTDHGKDQEIVLTPQQKQEIIQLTADKFSTWDWNYG
RSPEFNFKNHAKYDGGIIDVQVQAEGGTIRDINFTGDFLGLRDWREIKDQFIGLPFETVEIKPVFERNHEGRYFGNITDD
ELIKLFEA

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=318, Percent_Identity=28.6163522012579, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729884, Length=318, Percent_Identity=28.6163522012579, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729882, Length=318, Percent_Identity=28.6163522012579, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729880, Length=318, Percent_Identity=28.6163522012579, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729878, Length=318, Percent_Identity=28.6163522012579, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790846, Length=277, Percent_Identity=31.7689530685921, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71981909, Length=258, Percent_Identity=29.4573643410853, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=290, Percent_Identity=25.1724137931034, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573464, Length=287, Percent_Identity=33.4494773519164, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37163; Mature: 37163

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYVTYMGTDAYTNIATDTWLLNNLNAKEPVFALWQNNNAIIVGQNQNTFGEVNQDFVDE
CEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
HQIEVVRRVTGGGAVYHDLGNLNFTLFVPIESAANVDFKKFSEPVLRALHTLGIDAEASG
HHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
RNDLLVNGKKISGVAQRYAKGYLMHHGTLLFDSNVDTMVRSLNVADEKFISKAAKSVRSR
CCCEEEECHHHHHHHHHHHCCEEEECCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHH
VGMIKDAAPKGLTLQDFWDAVQYELTDHGKDQEIVLTPQQKQEIIQLTADKFSTWDWNYG
HCHHHCCCCCCCCHHHHHHHHHEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCC
RSPEFNFKNHAKYDGGIIDVQVQAEGGTIRDINFTGDFLGLRDWREIKDQFIGLPFETVE
CCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
IKPVFERNHEGRYFGNITDDELIKLFEA
EEEHHHCCCCCCEECCCCHHHHHHHHCC
>Mature Secondary Structure
MRYVTYMGTDAYTNIATDTWLLNNLNAKEPVFALWQNNNAIIVGQNQNTFGEVNQDFVDE
CEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
HQIEVVRRVTGGGAVYHDLGNLNFTLFVPIESAANVDFKKFSEPVLRALHTLGIDAEASG
HHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
RNDLLVNGKKISGVAQRYAKGYLMHHGTLLFDSNVDTMVRSLNVADEKFISKAAKSVRSR
CCCEEEECHHHHHHHHHHHCCEEEECCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHH
VGMIKDAAPKGLTLQDFWDAVQYELTDHGKDQEIVLTPQQKQEIIQLTADKFSTWDWNYG
HCHHHCCCCCCCCHHHHHHHHHEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCC
RSPEFNFKNHAKYDGGIIDVQVQAEGGTIRDINFTGDFLGLRDWREIKDQFIGLPFETVE
CCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
IKPVFERNHEGRYFGNITDDELIKLFEA
EEEHHHCCCCCCEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]