Click here to switch to the map view.

The map label for this gene is adaB [H]

Identifier: 116493446

GI number: 116493446

Start: 1694891

End: 1695388

Strand: Reverse

Name: adaB [H]

Synonym: PEPE_1718

Alternate gene names: 116493446

Gene position: 1695388-1694891 (Counterclockwise)

Preceding gene: 116493447

Following gene: 116493445

Centisome position: 92.52

GC content: 36.14

Gene sequence:

>498_bases
ATGAAACTATATTATTCTAAATTTCAAGTGAAAGATGCAACGTACTATGTGGCATCGACTAAAAAGGGCTTAGGTTTTGT
CGGCTCACCGAACCAAACGTTAAATGAAGTAAATCAGTTTTATCAAGCAATTGAATTAATTGGAGATGAAAAATCAAATA
TCGAAGCTGTACGTCAAATCAAAGAATATCTTGAAAACCGTCGCCAAGAATTTAATTTAGCAATTGATCTAAGTAATGGA
ACCCAGTTTCAACAAAAGATATGGAAAACATTAACGAAAATTACGTACGGTCGAGGGGTTAATTATACGGAACTAGAAGA
ACTAGCAGAAACACCTAAAGCCATTCGAGCCGTTGCTAGTGCAGTGGCGCGTAATCCGATTTTGCTAGTCATTCCTTGTC
ACCGCGTGATTCGTAAAGATGGGAAAATTGGAAATTATCGTGCAGGGGTAGAATTAAAAGAAAAACTGCTGAAAATGGAA
GGTTTTCTTAAAGATTAA

Upstream 100 bases:

>100_bases
TTAAAAACTTTAAGAGTTAATGGTCAAGGATTCGTCCTTGGCTATTAACTTTTTTTATTTTACGATGATTACAGGTTAAA
ATCAGAAGGAGGGTGATGCT

Downstream 100 bases:

>100_bases
TAAAATCCAGCCCTAGTAACGGGTGTTTTGAACGAAATTTGCTAGACTGGTAAAAAAGACAGTAAAAAGGGGAGATTCAC
CAATGAAAATTTTAGTTGTT

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKLYYSKFQVKDATYYVASTKKGLGFVGSPNQTLNEVNQFYQAIELIGDEKSNIEAVRQIKEYLENRRQEFNLAIDLSNG
TQFQQKIWKTLTKITYGRGVNYTELEELAETPKAIRAVASAVARNPILLVIPCHRVIRKDGKIGNYRAGVELKEKLLKME
GFLKD

Sequences:

>Translated_165_residues
MKLYYSKFQVKDATYYVASTKKGLGFVGSPNQTLNEVNQFYQAIELIGDEKSNIEAVRQIKEYLENRRQEFNLAIDLSNG
TQFQQKIWKTLTKITYGRGVNYTELEELAETPKAIRAVASAVARNPILLVIPCHRVIRKDGKIGNYRAGVELKEKLLKME
GFLKD
>Mature_165_residues
MKLYYSKFQVKDATYYVASTKKGLGFVGSPNQTLNEVNQFYQAIELIGDEKSNIEAVRQIKEYLENRRQEFNLAIDLSNG
TQFQQKIWKTLTKITYGRGVNYTELEELAETPKAIRAVASAVARNPILLVIPCHRVIRKDGKIGNYRAGVELKEKLLKME
GFLKD

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=78, Percent_Identity=44.8717948717949, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=148, Percent_Identity=37.1621621621622, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=40, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=39.5061728395062, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=39.5061728395062, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=35.8024691358025, Blast_Score=68, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17137554, Length=139, Percent_Identity=37.410071942446, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18881; Mature: 18881

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYYSKFQVKDATYYVASTKKGLGFVGSPNQTLNEVNQFYQAIELIGDEKSNIEAVRQI
CCCCCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KEYLENRRQEFNLAIDLSNGTQFQQKIWKTLTKITYGRGVNYTELEELAETPKAIRAVAS
HHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHH
AVARNPILLVIPCHRVIRKDGKIGNYRAGVELKEKLLKMEGFLKD
HHHCCCEEEEECCHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLYYSKFQVKDATYYVASTKKGLGFVGSPNQTLNEVNQFYQAIELIGDEKSNIEAVRQI
CCCCCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KEYLENRRQEFNLAIDLSNGTQFQQKIWKTLTKITYGRGVNYTELEELAETPKAIRAVAS
HHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHH
AVARNPILLVIPCHRVIRKDGKIGNYRAGVELKEKLLKMEGFLKD
HHHCCCEEEEECCHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]