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The map label for this gene is vanS [H]

Identifier: 116493444

GI number: 116493444

Start: 1692971

End: 1694107

Strand: Reverse

Name: vanS [H]

Synonym: PEPE_1716

Alternate gene names: 116493444

Gene position: 1694107-1692971 (Counterclockwise)

Preceding gene: 116493445

Following gene: 116493443

Centisome position: 92.45

GC content: 36.85

Gene sequence:

>1137_bases
ATGAAGTTAACGGGGCGTGAAAAATATGAATTGTTTGGTGAAGGAGTTATTACGGTAATTCTCTTACTATTACTCAATTT
ATCAGTTTTTATCTTAATTGATAGTGCCTTAAAGTCGAATCCAGCTTTGTCAAACGCGGTCTTTATGGTTAAAAATTCAA
TTACAATTGGCGGCGTACAGATTTGGAGCTACCAAAAGATTTTTATTTTAATCATGTTTTTGGCTGATGCAGGGGTACTT
TATTGGCGCTTAGAACGACGTTACAAGCAAATGCAGTTACGTCATATTATTGCCGAATTACATTACATTGCTAATGGTCA
TTTTGATCATCGTATTCCATTTGAATTAAGTGGAGACCATCAACGAGTGGTGGATAGTGTGAACTCACTAGTTGACAGTG
TCATTAGCTCCATGGAAGAAGAGCGAGCGCTTAAGCAATCTAAAGATGATTTAATTACCAATGTATCGCATGATTTACGT
ACGCCATTAACCTCAATTATTGGTTATCTAAGGTTAATTGAAGATCGTCAATTTAAAAATGAAGATGACATTTTAAAATA
TACGCATACAGCATATTTAAAATCAATTCAGATGAAATCACTGGTAGAGGACCTTTTTGAATATACCAAGGTTAGTCAAT
CTAATCCCCATTTGACAATTAATACGATTAATGTGGATTCTATGCTCCAACAGTTAGCAGCTAGTTTTGAACTGGAAGCT
AAGCAGAAGCATTTTGTGATTACTTCTCAATGTACGCCGAAAGATTTAGAAATTACCGGGGATGCCGAAAAAATTGGTCG
GGTGTTTAACAATTTGATAACCAATGCCTTGAAATATGGTAATGGTGGTAAAAATATTTTTTTGAGTGCCAAACAAATTA
ATGATACGGTAATTTTTGAAGTTGCTAACGATGGTGAAAAAGTACCTGCTGAGGCCCTTGGCAAGCTTTTTGACCGCTTT
TATCGAGTGGAAGGTTCCCGTTCCAAAGCCACTGGAGGAACAGGACTTGGATTAGCTATTGCTCAAAGCATCGTTGAAAT
GCATAATGGTACTATCGAAGCTCATTCGAACGATAAGCGAACGAGCTTTGTGATTCAGTTGCCATTGCATCAACCGGTAA
GTAAGGACTTACCATAG

Upstream 100 bases:

>100_bases
ATTTACGCGACAAGATTGAAGAAGCTACGGGTGGTGAAAAGGTTATTGAAACCGTCTGGGGCGTTGGATATAAAGTAGTC
GCGCACTAGGAGAGATTCAA

Downstream 100 bases:

>100_bases
AAAATTTAAAAGATGTAAAGACTGTGATGAAAGATTTGAAGGATGGTTTAGAGCTATTCGTCAAAGAGCTACGCTTCAAT
TGTGGCGACTAAATTTTAAG

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKLTGREKYELFGEGVITVILLLLLNLSVFILIDSALKSNPALSNAVFMVKNSITIGGVQIWSYQKIFILIMFLADAGVL
YWRLERRYKQMQLRHIIAELHYIANGHFDHRIPFELSGDHQRVVDSVNSLVDSVISSMEEERALKQSKDDLITNVSHDLR
TPLTSIIGYLRLIEDRQFKNEDDILKYTHTAYLKSIQMKSLVEDLFEYTKVSQSNPHLTINTINVDSMLQQLAASFELEA
KQKHFVITSQCTPKDLEITGDAEKIGRVFNNLITNALKYGNGGKNIFLSAKQINDTVIFEVANDGEKVPAEALGKLFDRF
YRVEGSRSKATGGTGLGLAIAQSIVEMHNGTIEAHSNDKRTSFVIQLPLHQPVSKDLP

Sequences:

>Translated_378_residues
MKLTGREKYELFGEGVITVILLLLLNLSVFILIDSALKSNPALSNAVFMVKNSITIGGVQIWSYQKIFILIMFLADAGVL
YWRLERRYKQMQLRHIIAELHYIANGHFDHRIPFELSGDHQRVVDSVNSLVDSVISSMEEERALKQSKDDLITNVSHDLR
TPLTSIIGYLRLIEDRQFKNEDDILKYTHTAYLKSIQMKSLVEDLFEYTKVSQSNPHLTINTINVDSMLQQLAASFELEA
KQKHFVITSQCTPKDLEITGDAEKIGRVFNNLITNALKYGNGGKNIFLSAKQINDTVIFEVANDGEKVPAEALGKLFDRF
YRVEGSRSKATGGTGLGLAIAQSIVEMHNGTIEAHSNDKRTSFVIQLPLHQPVSKDLP
>Mature_378_residues
MKLTGREKYELFGEGVITVILLLLLNLSVFILIDSALKSNPALSNAVFMVKNSITIGGVQIWSYQKIFILIMFLADAGVL
YWRLERRYKQMQLRHIIAELHYIANGHFDHRIPFELSGDHQRVVDSVNSLVDSVISSMEEERALKQSKDDLITNVSHDLR
TPLTSIIGYLRLIEDRQFKNEDDILKYTHTAYLKSIQMKSLVEDLFEYTKVSQSNPHLTINTINVDSMLQQLAASFELEA
KQKHFVITSQCTPKDLEITGDAEKIGRVFNNLITNALKYGNGGKNIFLSAKQINDTVIFEVANDGEKVPAEALGKLFDRF
YRVEGSRSKATGGTGLGLAIAQSIVEMHNGTIEAHSNDKRTSFVIQLPLHQPVSKDLP

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=293, Percent_Identity=29.3515358361775, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1786783, Length=286, Percent_Identity=27.972027972028, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=28.2258064516129, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=28.2051282051282, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=27.7533039647577, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=27.7533039647577, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789149, Length=357, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789808, Length=240, Percent_Identity=31.25, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787894, Length=264, Percent_Identity=26.8939393939394, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788713, Length=241, Percent_Identity=24.896265560166, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=361, Percent_Identity=20.7756232686981, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081816, Length=249, Percent_Identity=26.1044176706827, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=267, Percent_Identity=24.3445692883895, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=260, Percent_Identity=25.7692307692308, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=23.5294117647059, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082128, Length=230, Percent_Identity=22.6086956521739, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790551, Length=238, Percent_Identity=24.7899159663866, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=23.75, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790300, Length=261, Percent_Identity=23.7547892720307, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42582; Mature: 42582

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTGREKYELFGEGVITVILLLLLNLSVFILIDSALKSNPALSNAVFMVKNSITIGGVQ
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCEE
IWSYQKIFILIMFLADAGVLYWRLERRYKQMQLRHIIAELHYIANGHFDHRIPFELSGDH
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
QRVVDSVNSLVDSVISSMEEERALKQSKDDLITNVSHDLRTPLTSIIGYLRLIEDRQFKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
EDDILKYTHTAYLKSIQMKSLVEDLFEYTKVSQSNPHLTINTINVDSMLQQLAASFELEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCCCC
KQKHFVITSQCTPKDLEITGDAEKIGRVFNNLITNALKYGNGGKNIFLSAKQINDTVIFE
CCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEE
VANDGEKVPAEALGKLFDRFYRVEGSRSKATGGTGLGLAIAQSIVEMHNGTIEAHSNDKR
ECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
TSFVIQLPLHQPVSKDLP
EEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKLTGREKYELFGEGVITVILLLLLNLSVFILIDSALKSNPALSNAVFMVKNSITIGGVQ
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCEE
IWSYQKIFILIMFLADAGVLYWRLERRYKQMQLRHIIAELHYIANGHFDHRIPFELSGDH
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
QRVVDSVNSLVDSVISSMEEERALKQSKDDLITNVSHDLRTPLTSIIGYLRLIEDRQFKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
EDDILKYTHTAYLKSIQMKSLVEDLFEYTKVSQSNPHLTINTINVDSMLQQLAASFELEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCCCC
KQKHFVITSQCTPKDLEITGDAEKIGRVFNNLITNALKYGNGGKNIFLSAKQINDTVIFE
CCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEE
VANDGEKVPAEALGKLFDRFYRVEGSRSKATGGTGLGLAIAQSIVEMHNGTIEAHSNDKR
ECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
TSFVIQLPLHQPVSKDLP
EEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]