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The map label for this gene is dacA [H]

Identifier: 116493443

GI number: 116493443

Start: 1691534

End: 1692790

Strand: Reverse

Name: dacA [H]

Synonym: PEPE_1715

Alternate gene names: 116493443

Gene position: 1692790-1691534 (Counterclockwise)

Preceding gene: 116493444

Following gene: 116493442

Centisome position: 92.38

GC content: 36.83

Gene sequence:

>1257_bases
ATGAAAAAATTATACACGATGCTGATTGCGTTTTTAATGATGATGACGAGTTTTGCGCCAGCGCTAGCTGCTGAAAATAA
CGAATCAGTTATTGAAGCAACCACTAGTAATATCACTGCTAAATCAGCGATTGCAGTTGATGAAAACACAGGCCAGATTC
TATATAGCAAGGATGAAAACACAGTTCGTCCGATTGCCTCAATGTCGAAGATTGTGGCAGCTTACCTCATCATGAAGAGC
GTTAAAGAGGGCCAAATGCATTGGAATCAAAAAGTGTCTATCAATAAGAGCTTGGAAGAAGTTAGTAACAACAGTGAATT
AACCAACGTTCCGCTTTCTTCTAATCAAACTTATACAGTGCGTAACTTGTTAGATGCATCATTAATTTATTCAGCTAACG
CGGCAATTTTATCTTTAGGAAAAGCCCAAGCAGGAACTTCGGAAAAATTTGTCAATGAAATGCGGTCAACTGTGAAAAAA
TGGGGCATTAAAGATGCAAAATTGTACAACGCAGCGGGCTTATTAGAAAGCCAAGTTGGTAGTGAAAAGTACCCTGGTAC
ATCTGGAAATATAGAAAATGAAATGTCTGCTAAAGATATGGCAATCGTAATTTCCCATGTCTTAAAAGAATTCCCTGAGA
TTCTAAATATCACTAAAATCCAAAAAACAACTTTTAATACTGGAAAAGAAGAACTTGAAATTACTAATCATAATGAATTA
CTAAAAGGCGCTGAACAGTATGAAGCAAAATACCAAATTGATGGTCTAAAAACAGGTACATCAGATAAAGCCGGAGAGGA
TTTTTCTGCCACCGGTAAAATTAAAGGTCACCGAGTAATCAGTGTTGTATTGGGTTCCGGTGAAAATCAACGTTTCACAG
ATACCAAAACGATTTGGAATGCGTTGATGACTAAGTTGGCCGTTTTAAGTTCCAATTTGAAGAATCAAGCCTCTGTTAAA
ATTAACTCAGCTAAAGTTGCGGATGTTAAATTAGAACTTACCCAACCGCTAGATTACTGGGTTCCAAAAGGTCAGAGCGA
AAAACTGCATGTTAATGCAATTAAATTAAATGATCCAAAACAAAAAGCACCCATTAAACATTCTAAGGTAATTGCGACGG
CGAATGTTAAAGGCAATCAGGACCAATTCCTTGAAAATGGTAAGGGTGTACATGTGAAATTGAAACCAACTCAAGACGTG
AAAAAAGCTAATATTTTTGTTCGTTTGGGGCGTTCTATAGCTGATTGGTTTAGTTAA

Upstream 100 bases:

>100_bases
TGTGGCGACTAAATTTTAAGAGATTAACTTGTTAGTTACCACATTGAAAAATAGACTTCCTTTTGGAACAGTCTTTTTTA
TTCGGATCAGGAGTTAGATT

Downstream 100 bases:

>100_bases
AGGACGCGATTAAAGAGGGATATTTAGTGGCTTTACTATTAAAATTTTATTTAGCGTGTTAGAATTAATTCGTATGCTTT
GCAAAAAATTAATGTATTCT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKKLYTMLIAFLMMMTSFAPALAAENNESVIEATTSNITAKSAIAVDENTGQILYSKDENTVRPIASMSKIVAAYLIMKS
VKEGQMHWNQKVSINKSLEEVSNNSELTNVPLSSNQTYTVRNLLDASLIYSANAAILSLGKAQAGTSEKFVNEMRSTVKK
WGIKDAKLYNAAGLLESQVGSEKYPGTSGNIENEMSAKDMAIVISHVLKEFPEILNITKIQKTTFNTGKEELEITNHNEL
LKGAEQYEAKYQIDGLKTGTSDKAGEDFSATGKIKGHRVISVVLGSGENQRFTDTKTIWNALMTKLAVLSSNLKNQASVK
INSAKVADVKLELTQPLDYWVPKGQSEKLHVNAIKLNDPKQKAPIKHSKVIATANVKGNQDQFLENGKGVHVKLKPTQDV
KKANIFVRLGRSIADWFS

Sequences:

>Translated_418_residues
MKKLYTMLIAFLMMMTSFAPALAAENNESVIEATTSNITAKSAIAVDENTGQILYSKDENTVRPIASMSKIVAAYLIMKS
VKEGQMHWNQKVSINKSLEEVSNNSELTNVPLSSNQTYTVRNLLDASLIYSANAAILSLGKAQAGTSEKFVNEMRSTVKK
WGIKDAKLYNAAGLLESQVGSEKYPGTSGNIENEMSAKDMAIVISHVLKEFPEILNITKIQKTTFNTGKEELEITNHNEL
LKGAEQYEAKYQIDGLKTGTSDKAGEDFSATGKIKGHRVISVVLGSGENQRFTDTKTIWNALMTKLAVLSSNLKNQASVK
INSAKVADVKLELTQPLDYWVPKGQSEKLHVNAIKLNDPKQKAPIKHSKVIATANVKGNQDQFLENGKGVHVKLKPTQDV
KKANIFVRLGRSIADWFS
>Mature_418_residues
MKKLYTMLIAFLMMMTSFAPALAAENNESVIEATTSNITAKSAIAVDENTGQILYSKDENTVRPIASMSKIVAAYLIMKS
VKEGQMHWNQKVSINKSLEEVSNNSELTNVPLSSNQTYTVRNLLDASLIYSANAAILSLGKAQAGTSEKFVNEMRSTVKK
WGIKDAKLYNAAGLLESQVGSEKYPGTSGNIENEMSAKDMAIVISHVLKEFPEILNITKIQKTTFNTGKEELEITNHNEL
LKGAEQYEAKYQIDGLKTGTSDKAGEDFSATGKIKGHRVISVVLGSGENQRFTDTKTIWNALMTKLAVLSSNLKNQASVK
INSAKVADVKLELTQPLDYWVPKGQSEKLHVNAIKLNDPKQKAPIKHSKVIATANVKGNQDQFLENGKGVHVKLKPTQDV
KKANIFVRLGRSIADWFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=410, Percent_Identity=25.3658536585366, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786851, Length=405, Percent_Identity=24.6913580246914, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082022, Length=419, Percent_Identity=23.1503579952267, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082054, Length=301, Percent_Identity=25.2491694352159, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45928; Mature: 45928

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLYTMLIAFLMMMTSFAPALAAENNESVIEATTSNITAKSAIAVDENTGQILYSKDEN
CHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCEECEEEEEECCCCCEEEECCCC
TVRPIASMSKIVAAYLIMKSVKEGQMHWNQKVSINKSLEEVSNNSELTNVPLSSNQTYTV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEECCCCCCCCEEH
RNLLDASLIYSANAAILSLGKAQAGTSEKFVNEMRSTVKKWGIKDAKLYNAAGLLESQVG
HHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
SEKYPGTSGNIENEMSAKDMAIVISHVLKEFPEILNITKIQKTTFNTGKEELEITNHNEL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHEEECCCHHH
LKGAEQYEAKYQIDGLKTGTSDKAGEDFSATGKIKGHRVISVVLGSGENQRFTDTKTIWN
HHCHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ALMTKLAVLSSNLKNQASVKINSAKVADVKLELTQPLDYWVPKGQSEKLHVNAIKLNDPK
HHHHHHHHHHHCCCCCCEEEECCEEEEEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCH
QKAPIKHSKVIATANVKGNQDQFLENGKGVHVKLKPTQDVKKANIFVRLGRSIADWFS
HHCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHEEECHHHHHHCC
>Mature Secondary Structure
MKKLYTMLIAFLMMMTSFAPALAAENNESVIEATTSNITAKSAIAVDENTGQILYSKDEN
CHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCEECEEEEEECCCCCEEEECCCC
TVRPIASMSKIVAAYLIMKSVKEGQMHWNQKVSINKSLEEVSNNSELTNVPLSSNQTYTV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEECCCCCCCCEEH
RNLLDASLIYSANAAILSLGKAQAGTSEKFVNEMRSTVKKWGIKDAKLYNAAGLLESQVG
HHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
SEKYPGTSGNIENEMSAKDMAIVISHVLKEFPEILNITKIQKTTFNTGKEELEITNHNEL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHEEECCCHHH
LKGAEQYEAKYQIDGLKTGTSDKAGEDFSATGKIKGHRVISVVLGSGENQRFTDTKTIWN
HHCHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ALMTKLAVLSSNLKNQASVKINSAKVADVKLELTQPLDYWVPKGQSEKLHVNAIKLNDPK
HHHHHHHHHHHCCCCCCEEEECCEEEEEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCH
QKAPIKHSKVIATANVKGNQDQFLENGKGVHVKLKPTQDVKKANIFVRLGRSIADWFS
HHCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]