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The map label for this gene is purR [H]

Identifier: 116493428

GI number: 116493428

Start: 1674749

End: 1675747

Strand: Reverse

Name: purR [H]

Synonym: PEPE_1700

Alternate gene names: 116493428

Gene position: 1675747-1674749 (Counterclockwise)

Preceding gene: 116493429

Following gene: 116493427

Centisome position: 91.45

GC content: 36.04

Gene sequence:

>999_bases
ATGGAAACAAAAAAGATAACGATTAAAGATGTTGCTAGACAATCAGGAGTTTCAATCTCGACGGTTTCGCAAATCTTAAA
TGGAAATGAAACTCGGTTTAGTTCACTTACGGTAAAAAAGGTAATTCAGGCAAAAAAAGATTTAAATTATGAACCCGATT
ACTTTGCTCGTCGAATGATTATGAAAAAAAGTAAAACGATTGGTGTTTTAGTACCCGATATTACCAATCCGTTTTTTAGT
GAATTAGTTCGTGGAATTGAAAATATTTTTTATCAAAAGGGATTTATAACTATGCTTTGTAACGCAGATCTTGACCAGGA
AAAAGAAGATGCTTACTTAGCTGAATTGAGTCGGCGTGGGGTTGATGGGTTTATTATTGCTAGTTCAGTAATTTCAAATG
AATCTATTAATGCCACCTTAAAAGCTAAAAATCATCCATTTATTGTTCTTGATCAGAAAAAGGCTGATGGAATTAGTGAC
GCCGTTTTAACTGATGATTTTTCGGGTGGGGCTCAAGCCGCTCAACATTTGAAAGATTTAGGGCATCAAAAAATTGCCAT
AGTCATGCCAACTAATGCACCTGAAAATGTTTGTGGTCGTTTAGCTGGAGTACGTTCTGTATATGAAGAAAAACAAATTG
AAATTATCGATGCCCAGCTATCAAAAATTGGTGGTCGGGAAGCCGTTGCTAAAATTTTAGCAACGGATGTTACCGGAATA
TTTGCGGTCAATGACGAAATTGCCTTAGGAATTTATTTGGGATTAGCCGAAGCTGGCAAGCAGATTCCTGAAGATTATAG
TGTGATTGGCTACGATAATATTGATATGTGTGAATATATGGTTCCTAAACTGACCACTATTGCACAACCTATTTTTGAGT
TAGGTCAGACAACAGCTAACATGCTACTTGAAAGAATTGAAACTCCCGATAAGAACTGGGAAGAGAAGACTTTACCCGTT
CACTTAGTGAAAAGATTTTCCACAGCACATTTGATTTAA

Upstream 100 bases:

>100_bases
GTTTCAAAGATACTCATTTCTAAACGAAATGGGTATTTTTTTATCTTATGGTGTAAACTAAAAATATAAAAAATAACTGT
CTAGGTATTGGTGTGAAAAA

Downstream 100 bases:

>100_bases
TAGAATCAATGTGCTATATTTTTGATATGCTATTAAAACGTTTTCATAAAACGTTTTAAACAAGTAAAAAGATAATCGAA
AACGGAGGAATCCATCATGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
METKKITIKDVARQSGVSISTVSQILNGNETRFSSLTVKKVIQAKKDLNYEPDYFARRMIMKKSKTIGVLVPDITNPFFS
ELVRGIENIFYQKGFITMLCNADLDQEKEDAYLAELSRRGVDGFIIASSVISNESINATLKAKNHPFIVLDQKKADGISD
AVLTDDFSGGAQAAQHLKDLGHQKIAIVMPTNAPENVCGRLAGVRSVYEEKQIEIIDAQLSKIGGREAVAKILATDVTGI
FAVNDEIALGIYLGLAEAGKQIPEDYSVIGYDNIDMCEYMVPKLTTIAQPIFELGQTTANMLLERIETPDKNWEEKTLPV
HLVKRFSTAHLI

Sequences:

>Translated_332_residues
METKKITIKDVARQSGVSISTVSQILNGNETRFSSLTVKKVIQAKKDLNYEPDYFARRMIMKKSKTIGVLVPDITNPFFS
ELVRGIENIFYQKGFITMLCNADLDQEKEDAYLAELSRRGVDGFIIASSVISNESINATLKAKNHPFIVLDQKKADGISD
AVLTDDFSGGAQAAQHLKDLGHQKIAIVMPTNAPENVCGRLAGVRSVYEEKQIEIIDAQLSKIGGREAVAKILATDVTGI
FAVNDEIALGIYLGLAEAGKQIPEDYSVIGYDNIDMCEYMVPKLTTIAQPIFELGQTTANMLLERIETPDKNWEEKTLPV
HLVKRFSTAHLI
>Mature_332_residues
METKKITIKDVARQSGVSISTVSQILNGNETRFSSLTVKKVIQAKKDLNYEPDYFARRMIMKKSKTIGVLVPDITNPFFS
ELVRGIENIFYQKGFITMLCNADLDQEKEDAYLAELSRRGVDGFIIASSVISNESINATLKAKNHPFIVLDQKKADGISD
AVLTDDFSGGAQAAQHLKDLGHQKIAIVMPTNAPENVCGRLAGVRSVYEEKQIEIIDAQLSKIGGREAVAKILATDVTGI
FAVNDEIALGIYLGLAEAGKQIPEDYSVIGYDNIDMCEYMVPKLTTIAQPIFELGQTTANMLLERIETPDKNWEEKTLPV
HLVKRFSTAHLI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=34.9544072948328, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=35.6083086053413, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=31.8318318318318, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=29.7619047619048, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=28.0405405405405, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789068, Length=330, Percent_Identity=28.1818181818182, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=29.7297297297297, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787906, Length=340, Percent_Identity=25.8823529411765, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=26.7080745341615, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=28.6163522012579, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790689, Length=305, Percent_Identity=24.9180327868852, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=19.2429022082019, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790715, Length=127, Percent_Identity=24.4094488188976, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36699; Mature: 36699

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKITIKDVARQSGVSISTVSQILNGNETRFSSLTVKKVIQAKKDLNYEPDYFARRMI
CCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MKKSKTIGVLVPDITNPFFSELVRGIENIFYQKGFITMLCNADLDQEKEDAYLAELSRRG
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCC
VDGFIIASSVISNESINATLKAKNHPFIVLDQKKADGISDAVLTDDFSGGAQAAQHLKDL
CCEEEEHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHC
GHQKIAIVMPTNAPENVCGRLAGVRSVYEEKQIEIIDAQLSKIGGREAVAKILATDVTGI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
FAVNDEIALGIYLGLAEAGKQIPEDYSVIGYDNIDMCEYMVPKLTTIAQPIFELGQTTAN
EEECCCEEEHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLERIETPDKNWEEKTLPVHLVKRFSTAHLI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
METKKITIKDVARQSGVSISTVSQILNGNETRFSSLTVKKVIQAKKDLNYEPDYFARRMI
CCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MKKSKTIGVLVPDITNPFFSELVRGIENIFYQKGFITMLCNADLDQEKEDAYLAELSRRG
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCC
VDGFIIASSVISNESINATLKAKNHPFIVLDQKKADGISDAVLTDDFSGGAQAAQHLKDL
CCEEEEHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHC
GHQKIAIVMPTNAPENVCGRLAGVRSVYEEKQIEIIDAQLSKIGGREAVAKILATDVTGI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
FAVNDEIALGIYLGLAEAGKQIPEDYSVIGYDNIDMCEYMVPKLTTIAQPIFELGQTTAN
EEECCCEEEHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLERIETPDKNWEEKTLPVHLVKRFSTAHLI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA