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The map label for this gene is chrR [H]

Identifier: 116492889

GI number: 116492889

Start: 1126431

End: 1127168

Strand: Reverse

Name: chrR [H]

Synonym: PEPE_1127

Alternate gene names: 116492889

Gene position: 1127168-1126431 (Counterclockwise)

Preceding gene: 116492890

Following gene: 116492887

Centisome position: 61.51

GC content: 36.18

Gene sequence:

>738_bases
ATGCGAGATACAATTATTTTAATGCAACATCATACATCCGTGCGTAACTTTATGCCGGAACCACTTCCTGAAAGTGTAAA
AAATCAATTAGTTTCGGCCGCTCAAAGTGGTTCATCTTCGAACTTTATTCAAGCAACTTCTATTATTGAAGTGACTGATC
TTAAAACTCGTGAAAAAATCGCCAAAATTTCCAATAGTGATGCATACGTAAAACAAAGTGGGGCCTTTTTTGTTTTTATA
GCAGATTTATATCGACAAGCTAATATCCTAAAGCAAGCCCAATTACCATTAGACGGGATTCAAAATATGGAATCTCTTCT
AGTAGGAGTTGTTGATACGGCCATTGCAGGTGAGAACATGGCGATTGCTGCTGAGTCCCTAGACTTAGGAATCTGTTTTA
TTGGTGGAATCCGAAATGAAATGGATAGCATTAAGGAACTACTTGGTTTACCTAAATATACAGTGTCACTTTTTGGAATG
ACTGTGGGTATTCCAGTTCAAAAAAATGAAATTAAGCCACGTCTACCTAAAAAGAATATTTCTTTTAAAAACCGATATGA
ACCATCGGTTGCAGTAAATCTGGATAGTTATAATCAACAAACTCGTGTGTATTATGCTAATCGTTCAACTGCTCCCCAAA
ATACGGATTGGTCTCATAAAATGCTTCATTTTTTTGCCGAACCCAAAAGACCAGCAGTTGCTGAATTTCTTAAAAAACAA
GGATTTTCACTGGAATAA

Upstream 100 bases:

>100_bases
CAAATGAGCTTAAACTAACTTTTTAAAAGTGTGCTAGAATTAAATATTATTTTTGAATTGCCTAAAAAGATTATTTAAGT
TTAAAAAGAAGGATTTAATA

Downstream 100 bases:

>100_bases
AAAATGCATTACTAGTTTTAAAACTAGTAGTGCATTTTTATTAAACAATCTTTTTTCTAGTAGGTAAGGCTTGTAGAAAT
GCCGTAGTTAAAATTAATAA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRDTIILMQHHTSVRNFMPEPLPESVKNQLVSAAQSGSSSNFIQATSIIEVTDLKTREKIAKISNSDAYVKQSGAFFVFI
ADLYRQANILKQAQLPLDGIQNMESLLVGVVDTAIAGENMAIAAESLDLGICFIGGIRNEMDSIKELLGLPKYTVSLFGM
TVGIPVQKNEIKPRLPKKNISFKNRYEPSVAVNLDSYNQQTRVYYANRSTAPQNTDWSHKMLHFFAEPKRPAVAEFLKKQ
GFSLE

Sequences:

>Translated_245_residues
MRDTIILMQHHTSVRNFMPEPLPESVKNQLVSAAQSGSSSNFIQATSIIEVTDLKTREKIAKISNSDAYVKQSGAFFVFI
ADLYRQANILKQAQLPLDGIQNMESLLVGVVDTAIAGENMAIAAESLDLGICFIGGIRNEMDSIKELLGLPKYTVSLFGM
TVGIPVQKNEIKPRLPKKNISFKNRYEPSVAVNLDSYNQQTRVYYANRSTAPQNTDWSHKMLHFFAEPKRPAVAEFLKKQ
GFSLE
>Mature_245_residues
MRDTIILMQHHTSVRNFMPEPLPESVKNQLVSAAQSGSSSNFIQATSIIEVTDLKTREKIAKISNSDAYVKQSGAFFVFI
ADLYRQANILKQAQLPLDGIQNMESLLVGVVDTAIAGENMAIAAESLDLGICFIGGIRNEMDSIKELLGLPKYTVSLFGM
TVGIPVQKNEIKPRLPKKNISFKNRYEPSVAVNLDSYNQQTRVYYANRSTAPQNTDWSHKMLHFFAEPKRPAVAEFLKKQ
GFSLE

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=243, Percent_Identity=37.4485596707819, Blast_Score=166, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27344; Mature: 27344

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDTIILMQHHTSVRNFMPEPLPESVKNQLVSAAQSGSSSNFIQATSIIEVTDLKTREKI
CCCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHH
AKISNSDAYVKQSGAFFVFIADLYRQANILKQAQLPLDGIQNMESLLVGVVDTAIAGENM
HHCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
AIAAESLDLGICFIGGIRNEMDSIKELLGLPKYTVSLFGMTVGIPVQKNEIKPRLPKKNI
EEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCC
SFKNRYEPSVAVNLDSYNQQTRVYYANRSTAPQNTDWSHKMLHFFAEPKRPAVAEFLKKQ
CCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHC
GFSLE
CCCCC
>Mature Secondary Structure
MRDTIILMQHHTSVRNFMPEPLPESVKNQLVSAAQSGSSSNFIQATSIIEVTDLKTREKI
CCCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHH
AKISNSDAYVKQSGAFFVFIADLYRQANILKQAQLPLDGIQNMESLLVGVVDTAIAGENM
HHCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
AIAAESLDLGICFIGGIRNEMDSIKELLGLPKYTVSLFGMTVGIPVQKNEIKPRLPKKNI
EEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCC
SFKNRYEPSVAVNLDSYNQQTRVYYANRSTAPQNTDWSHKMLHFFAEPKRPAVAEFLKKQ
CCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHC
GFSLE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA