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The map label for this gene is dnaG [H]

Identifier: 116492862

GI number: 116492862

Start: 1098480

End: 1100342

Strand: Reverse

Name: dnaG [H]

Synonym: PEPE_1100

Alternate gene names: 116492862

Gene position: 1100342-1098480 (Counterclockwise)

Preceding gene: 116492863

Following gene: 116492861

Centisome position: 60.05

GC content: 34.62

Gene sequence:

>1863_bases
ATGGCTGGAATGATTCCAGAAGATGTAATTGATGATGTTAGAACATCTGTTGACATCGCTGATGTTGTTGGTAGATATGT
CCAGTTAAGAAAGGCGGGTAAAAACCTATTTGGTGTTTGTCCATTTCATGATGAGAAGACACCGTCTTTTTCGGTTTCGG
AGAGTAAACAAATTTTTCATTGTTTTAGTTGCGGACGCGGTGGAAATGTTTTTAAATTTTTGATGGATTTAGAGAATCTT
AGTTTCCCTGAGTCAGTTCGTAGAGTAGCGGAATTTGGCAATATTCCGTTAGATCAGAGTTATTTCAAGTCAACTAAAAA
GTCGTCGTATACGTCTGAACAAAGTGCATTGATTGATTTATATGAGAAAGCTTCTGAGCTATTTAGTCATATTCTATTGA
ACACCAAATTAGGTGAAGATGCATTACAGTATTTGAAAAAACGAGGAATGACCGAGGATGATATCAAGAATTTTGATATT
GGATTTGCCGCCCCGGAAGACAATCTCCTGCTAAGTTTTTTTAAAGAACATAAGATTGACCAACATTTAATGGCTGAATC
AGGATTGTTTATTGAATCCGAAAATCAAACATTAAGAGATCGATTTTTTAACCGTATTATTTTTCCGATTAAAGATACAA
ATGGAAAAATCGTTGCATTTTCGGGCCGTAGTTTGGTTAAGGATAAGAACCATCCTAAATATTTAAATAGCCCTGAAACA
AGCATTTTTAATAAAGGAAAGGTGCTTTACAATTTTTCTCAAGCTCGTTCAGAAATTATCCAACAGAAAACAGTCATATT
ATATGAAGGTTTTATGGATGTCATTGCTGCTCATCGAGCGGCTGTAAAGAATGGAATTGCGACGATGGGAACCAGTTTGA
CTGAGGATCATATTAATGCTATCAAGCGGGTGGCTGATCGCGTAATAATCTGTTTTGATGGGGATGCGCCTGGACAAAAG
GCAATTGCTAGAGCAATTGAGTTATTGAGGCAATCTAGTGATTTACAAATTAGTGTGGTTTTTATTCCTGATAATTTAGA
TCCGGATGAGTATGTGAATCAAAGAGGGACAGCTGCATTTCAAAATTTGATGCAGCAGACTGGAGAATCAAGTACTTCAT
TTCTTTTGAAATATGATCGAACCGGTCTAAATCTAGCTAATGAAAATGATCAATTAGAATACTTAAAACGAGCTTTAAAA
CGAATTAGTAGTCTGAAAGATGCCCTAGAACAAGATTTGTATTTAAATCAATTATCAGATGAATTTAAGATTGATAAGCG
TGATTTAAAAGACCAGTTAAGAGAAAGTGTAGCAGATCGAATCGCGCATGCACCCAAACAGTTTGAATTGGAAGCGTCGA
TGCCTCCACCTCCACCGGAGGCAATGGGAGATCAACCCGTATTCAATCAAAATATTCAAAAAAAGTTATCTAAGATAGAT
ATTTCGGAATATCGTTTGCTAGCTCGTGCATTAAATAATCATGATGTTTGGCTAAAATTACAGAGCCTACCCAATTTTTA
TTTTCCAAATGAAAAGCTTGAAACAATTTATGTCTTGGCTAGTGGATATTTAGAAAAAAATCATGAATATAATGTTGCAG
ATTTTATGGATTATCTTAAAGAAGATGAGCTACAAGCAGTCGTTGCTGATATTGAATCAATTAACCTAAGCCCTGAATTT
ACTGAAGAAGAAATTCAAGATTATCTTAGCGTGATTGAAAATTTTGCTCCTTTAAGTGAACAAATTTCAAATGTTAAATT
GGATTTAGATGAAGCTACTAGATTAGGTAACAAAGAATTACAACAACAACTAACTTTAAAACTTATCGCGCTATATCAAA
AACAACAACAGCTTCAAAAATAA

Upstream 100 bases:

>100_bases
TGACAAAAGTGTTAAAAACAAGGGCGTTTTAATTGATTCTATTGCTCGACTGCTGTATCATACTAACGTATGAGTTTATC
TTGCGAAAGCTGGTGAGAGT

Downstream 100 bases:

>100_bases
TAGGGGGATCAGCATAAATGGCAAACAAAAAAACTGAAACAAAAAAGAAAAAGAGTGAAAGTATCATTTCTTCACCTGAG
TTTAAAAAAGCTGTCAATCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MAGMIPEDVIDDVRTSVDIADVVGRYVQLRKAGKNLFGVCPFHDEKTPSFSVSESKQIFHCFSCGRGGNVFKFLMDLENL
SFPESVRRVAEFGNIPLDQSYFKSTKKSSYTSEQSALIDLYEKASELFSHILLNTKLGEDALQYLKKRGMTEDDIKNFDI
GFAAPEDNLLLSFFKEHKIDQHLMAESGLFIESENQTLRDRFFNRIIFPIKDTNGKIVAFSGRSLVKDKNHPKYLNSPET
SIFNKGKVLYNFSQARSEIIQQKTVILYEGFMDVIAAHRAAVKNGIATMGTSLTEDHINAIKRVADRVIICFDGDAPGQK
AIARAIELLRQSSDLQISVVFIPDNLDPDEYVNQRGTAAFQNLMQQTGESSTSFLLKYDRTGLNLANENDQLEYLKRALK
RISSLKDALEQDLYLNQLSDEFKIDKRDLKDQLRESVADRIAHAPKQFELEASMPPPPPEAMGDQPVFNQNIQKKLSKID
ISEYRLLARALNNHDVWLKLQSLPNFYFPNEKLETIYVLASGYLEKNHEYNVADFMDYLKEDELQAVVADIESINLSPEF
TEEEIQDYLSVIENFAPLSEQISNVKLDLDEATRLGNKELQQQLTLKLIALYQKQQQLQK

Sequences:

>Translated_620_residues
MAGMIPEDVIDDVRTSVDIADVVGRYVQLRKAGKNLFGVCPFHDEKTPSFSVSESKQIFHCFSCGRGGNVFKFLMDLENL
SFPESVRRVAEFGNIPLDQSYFKSTKKSSYTSEQSALIDLYEKASELFSHILLNTKLGEDALQYLKKRGMTEDDIKNFDI
GFAAPEDNLLLSFFKEHKIDQHLMAESGLFIESENQTLRDRFFNRIIFPIKDTNGKIVAFSGRSLVKDKNHPKYLNSPET
SIFNKGKVLYNFSQARSEIIQQKTVILYEGFMDVIAAHRAAVKNGIATMGTSLTEDHINAIKRVADRVIICFDGDAPGQK
AIARAIELLRQSSDLQISVVFIPDNLDPDEYVNQRGTAAFQNLMQQTGESSTSFLLKYDRTGLNLANENDQLEYLKRALK
RISSLKDALEQDLYLNQLSDEFKIDKRDLKDQLRESVADRIAHAPKQFELEASMPPPPPEAMGDQPVFNQNIQKKLSKID
ISEYRLLARALNNHDVWLKLQSLPNFYFPNEKLETIYVLASGYLEKNHEYNVADFMDYLKEDELQAVVADIESINLSPEF
TEEEIQDYLSVIENFAPLSEQISNVKLDLDEATRLGNKELQQQLTLKLIALYQKQQQLQK
>Mature_619_residues
AGMIPEDVIDDVRTSVDIADVVGRYVQLRKAGKNLFGVCPFHDEKTPSFSVSESKQIFHCFSCGRGGNVFKFLMDLENLS
FPESVRRVAEFGNIPLDQSYFKSTKKSSYTSEQSALIDLYEKASELFSHILLNTKLGEDALQYLKKRGMTEDDIKNFDIG
FAAPEDNLLLSFFKEHKIDQHLMAESGLFIESENQTLRDRFFNRIIFPIKDTNGKIVAFSGRSLVKDKNHPKYLNSPETS
IFNKGKVLYNFSQARSEIIQQKTVILYEGFMDVIAAHRAAVKNGIATMGTSLTEDHINAIKRVADRVIICFDGDAPGQKA
IARAIELLRQSSDLQISVVFIPDNLDPDEYVNQRGTAAFQNLMQQTGESSTSFLLKYDRTGLNLANENDQLEYLKRALKR
ISSLKDALEQDLYLNQLSDEFKIDKRDLKDQLRESVADRIAHAPKQFELEASMPPPPPEAMGDQPVFNQNIQKKLSKIDI
SEYRLLARALNNHDVWLKLQSLPNFYFPNEKLETIYVLASGYLEKNHEYNVADFMDYLKEDELQAVVADIESINLSPEFT
EEEIQDYLSVIENFAPLSEQISNVKLDLDEATRLGNKELQQQLTLKLIALYQKQQQLQK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=369, Percent_Identity=37.1273712737127, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70659; Mature: 70528

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGMIPEDVIDDVRTSVDIADVVGRYVQLRKAGKNLFGVCPFHDEKTPSFSVSESKQIFH
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHH
CFSCGRGGNVFKFLMDLENLSFPESVRRVAEFGNIPLDQSYFKSTKKSSYTSEQSALIDL
HHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
YEKASELFSHILLNTKLGEDALQYLKKRGMTEDDIKNFDIGFAAPEDNLLLSFFKEHKID
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCHH
QHLMAESGLFIESENQTLRDRFFNRIIFPIKDTNGKIVAFSGRSLVKDKNHPKYLNSPET
HHHHHHCCCEEECCCCHHHHHHHHHEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCH
SIFNKGKVLYNFSQARSEIIQQKTVILYEGFMDVIAAHRAAVKNGIATMGTSLTEDHINA
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
IKRVADRVIICFDGDAPGQKAIARAIELLRQSSDLQISVVFIPDNLDPDEYVNQRGTAAF
HHHHHCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCHHHH
QNLMQQTGESSTSFLLKYDRTGLNLANENDQLEYLKRALKRISSLKDALEQDLYLNQLSD
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFKIDKRDLKDQLRESVADRIAHAPKQFELEASMPPPPPEAMGDQPVFNQNIQKKLSKID
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ISEYRLLARALNNHDVWLKLQSLPNFYFPNEKLETIYVLASGYLEKNHEYNVADFMDYLK
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHH
EDELQAVVADIESINLSPEFTEEEIQDYLSVIENFAPLSEQISNVKLDLDEATRLGNKEL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCEECHHHHHHCCCHHH
QQQLTLKLIALYQKQQQLQK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGMIPEDVIDDVRTSVDIADVVGRYVQLRKAGKNLFGVCPFHDEKTPSFSVSESKQIFH
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHH
CFSCGRGGNVFKFLMDLENLSFPESVRRVAEFGNIPLDQSYFKSTKKSSYTSEQSALIDL
HHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
YEKASELFSHILLNTKLGEDALQYLKKRGMTEDDIKNFDIGFAAPEDNLLLSFFKEHKID
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCHH
QHLMAESGLFIESENQTLRDRFFNRIIFPIKDTNGKIVAFSGRSLVKDKNHPKYLNSPET
HHHHHHCCCEEECCCCHHHHHHHHHEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCH
SIFNKGKVLYNFSQARSEIIQQKTVILYEGFMDVIAAHRAAVKNGIATMGTSLTEDHINA
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
IKRVADRVIICFDGDAPGQKAIARAIELLRQSSDLQISVVFIPDNLDPDEYVNQRGTAAF
HHHHHCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCHHHH
QNLMQQTGESSTSFLLKYDRTGLNLANENDQLEYLKRALKRISSLKDALEQDLYLNQLSD
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFKIDKRDLKDQLRESVADRIAHAPKQFELEASMPPPPPEAMGDQPVFNQNIQKKLSKID
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ISEYRLLARALNNHDVWLKLQSLPNFYFPNEKLETIYVLASGYLEKNHEYNVADFMDYLK
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHH
EDELQAVVADIESINLSPEFTEEEIQDYLSVIENFAPLSEQISNVKLDLDEATRLGNKEL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCEECHHHHHHCCCHHH
QQQLTLKLIALYQKQQQLQK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927; 8914262 [H]