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The map label for this gene is glyS

Identifier: 116492863

GI number: 116492863

Start: 1100594

End: 1102666

Strand: Reverse

Name: glyS

Synonym: PEPE_1101

Alternate gene names: 116492863

Gene position: 1102666-1100594 (Counterclockwise)

Preceding gene: 116492864

Following gene: 116492862

Centisome position: 60.18

GC content: 36.85

Gene sequence:

>2073_bases
ATGGCACATAATTATTTACTAGAAATTGGATTGGAAGAAATTCCGGCCCATGTTGTAACTCCAAGTATCAAACAGTTAGT
ACAAAAAGTAACAGCCTTCTTAAAAGAAAATCGCTTAACATACGACTCAATTGATCATTTTTCAACTCCTCGTCGTTTGG
CAATTCGAATCAATGGGTTAGGCGACCAACAACCTGATATTGAAGAAGATGCTAAAGGCCCTGCTCGTAAAATTGCTCAA
GATGCTGATGGAAATTGGACTAAGGCTGCAATTGGCTTTACACGTGGACAAGGTCTTACGGTTGACGATATTACTTTTAA
AACAATCAAAGGTACGGACTATGTGTACGTCCATAAGTTAATCAAAGGAAAGATGACTAAGGAAATCCTTACGGGATTAA
AAGAAGTTGTTGAATCAATTAATTTCCCAACAATGATGAAGTGGGCTAACTTTGATTTTAAATATGTACGCCCAATTCGT
TGGCTGGTTTCTATTCTAGATGAAGAAGTCCTTCCTTTTAGTATCTTAGACGTAACTGCGGGACGCCGAACAGAAGGACA
TCGTTTCTTAGGTGAAGCTGTCGAACTGGCTAATGCTGAAGAATATGAAGCAAAATTACACGATCAATTTGTGATTGTTG
ATGCCGACGAGCGTAAACAATTAATTTCAAACCAAATTAAAGCAATTGCTGAAAGCAATCGTTGGAACGTTACCCCTAAC
CCAGGTCTTTTAGAAGAGGTTAACAATTTGGTTGAGTGGCCAACCGCTTTTAATGGGGGATTTGATGAAAAGTATTTAGC
TATTCCAGAAGAGGTATTGATAACATCAATGCGTGACCACCAACGCTTCTTCTTTGTCCGCGACCAAGCTGGAAAGCTAT
TGCCAAACTTCATCTCCGTACGAAATGGGAATGAAGAATTTATTGAAAATGTTGTTCGTGGAAATGAAAAAGTTTTAACT
GCACGTTTAGAAGACGCTGCTTTCTTCTACGAAGAAGATCAAAAACATGATATTAATTATTATGTTGACCGACTTAAAAA
GGTTAGTTTCCATGATAAGATTGGTTCAATGTACGAAAAACTGCAACGAGTTAATTCTATTGCTAAAGTTATTGGAAACA
CCTTAAATCTTAATCAAACGGAACTTGATGATATCGATCGCGCTACAATGATTTATAAATTTGATTTGGTAACTGGTATG
GTTGGTGAGTTCTCAGAATTACAAGGAGTAATGGGTGAAAAATATGCTCAACTTAATGGTGAAAACCAAGCAGTAGCCCA
AGCCATTCGCGAACATTACATGCCAAATAGCGCAGAAGGTGATTTGCCTGAAAGTGTAACGGGCGCGGTAGTCGCATTAG
CTGATAAGTTTGATAACATCTTTAGTTTTTTCTCAGCTGGTATGATTCCAAGTGGTTCAAACGATCCATATGCATTACGC
CGACATGCATATGGAATTGTTAGAATCTTAAATAGCCGTGATTGGCAATTAGATTTAAATCAATTCAAATCACAAGTTAA
GACTGAATTAGCGGAGAATGGCACAGCGTTTGGTGTGGATGTCGATCAAAACTTTGACCAAGTACTTAACTTCTTTAATG
ACCGTATTAAACAATTGCTTGATCATCAAAAGATTAGTCATGATATCGTTGAAACGGTGCTTACAGGTAATAATCATGAT
GTTACGGAAATTATCGAAGCTGCCCAAGTACTAGCAGATGCTAAAGCGAGCTCTACATTTAAAGATGATATTGAAGCTTT
AACACGAGTTCAAAGAATTGCTACAAAGAATGAAGAAAGTGGAGAACTTAATGTAGATCCACAATTATTTAATAATGCTT
CTGAAGGCGAACTTTTTGATCAAATTATTAAAATTGAAGCTGCAAATAATTTGACAATGAGCCAACTATTTGCTAAATTA
TGCGAGTTAACTCCTGCGATTAGCAAGTACTTTGACGCAACGATGGTCATGGACAAAGACGAAAATATTAAGCGTAATCG
TTTGAATATGATGAGTCGGTTAGCTAATTTAATTCTAAAAATTGGGGACCTAACTAACGTACTTGTAAAATAA

Upstream 100 bases:

>100_bases
GTATTCGTAATATGGCTCGTAAGGTAGCACGGGCATTTGTGGAAGAACGTGAAAAATTAGGATTCCCATTATTAAAAAAT
AACGAAGAGGAGGCAAAATA

Downstream 100 bases:

>100_bases
AAATGTTTTTATAAGGATGATTTGGATCTGCTTAAAGTTAATAAATAAATAAAACAAGCCATTTAGTCTAACGAGATTAA
ATGGCTTGTTTCTTCCTATA

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MAHNYLLEIGLEEIPAHVVTPSIKQLVQKVTAFLKENRLTYDSIDHFSTPRRLAIRINGLGDQQPDIEEDAKGPARKIAQ
DADGNWTKAAIGFTRGQGLTVDDITFKTIKGTDYVYVHKLIKGKMTKEILTGLKEVVESINFPTMMKWANFDFKYVRPIR
WLVSILDEEVLPFSILDVTAGRRTEGHRFLGEAVELANAEEYEAKLHDQFVIVDADERKQLISNQIKAIAESNRWNVTPN
PGLLEEVNNLVEWPTAFNGGFDEKYLAIPEEVLITSMRDHQRFFFVRDQAGKLLPNFISVRNGNEEFIENVVRGNEKVLT
ARLEDAAFFYEEDQKHDINYYVDRLKKVSFHDKIGSMYEKLQRVNSIAKVIGNTLNLNQTELDDIDRATMIYKFDLVTGM
VGEFSELQGVMGEKYAQLNGENQAVAQAIREHYMPNSAEGDLPESVTGAVVALADKFDNIFSFFSAGMIPSGSNDPYALR
RHAYGIVRILNSRDWQLDLNQFKSQVKTELAENGTAFGVDVDQNFDQVLNFFNDRIKQLLDHQKISHDIVETVLTGNNHD
VTEIIEAAQVLADAKASSTFKDDIEALTRVQRIATKNEESGELNVDPQLFNNASEGELFDQIIKIEAANNLTMSQLFAKL
CELTPAISKYFDATMVMDKDENIKRNRLNMMSRLANLILKIGDLTNVLVK

Sequences:

>Translated_690_residues
MAHNYLLEIGLEEIPAHVVTPSIKQLVQKVTAFLKENRLTYDSIDHFSTPRRLAIRINGLGDQQPDIEEDAKGPARKIAQ
DADGNWTKAAIGFTRGQGLTVDDITFKTIKGTDYVYVHKLIKGKMTKEILTGLKEVVESINFPTMMKWANFDFKYVRPIR
WLVSILDEEVLPFSILDVTAGRRTEGHRFLGEAVELANAEEYEAKLHDQFVIVDADERKQLISNQIKAIAESNRWNVTPN
PGLLEEVNNLVEWPTAFNGGFDEKYLAIPEEVLITSMRDHQRFFFVRDQAGKLLPNFISVRNGNEEFIENVVRGNEKVLT
ARLEDAAFFYEEDQKHDINYYVDRLKKVSFHDKIGSMYEKLQRVNSIAKVIGNTLNLNQTELDDIDRATMIYKFDLVTGM
VGEFSELQGVMGEKYAQLNGENQAVAQAIREHYMPNSAEGDLPESVTGAVVALADKFDNIFSFFSAGMIPSGSNDPYALR
RHAYGIVRILNSRDWQLDLNQFKSQVKTELAENGTAFGVDVDQNFDQVLNFFNDRIKQLLDHQKISHDIVETVLTGNNHD
VTEIIEAAQVLADAKASSTFKDDIEALTRVQRIATKNEESGELNVDPQLFNNASEGELFDQIIKIEAANNLTMSQLFAKL
CELTPAISKYFDATMVMDKDENIKRNRLNMMSRLANLILKIGDLTNVLVK
>Mature_689_residues
AHNYLLEIGLEEIPAHVVTPSIKQLVQKVTAFLKENRLTYDSIDHFSTPRRLAIRINGLGDQQPDIEEDAKGPARKIAQD
ADGNWTKAAIGFTRGQGLTVDDITFKTIKGTDYVYVHKLIKGKMTKEILTGLKEVVESINFPTMMKWANFDFKYVRPIRW
LVSILDEEVLPFSILDVTAGRRTEGHRFLGEAVELANAEEYEAKLHDQFVIVDADERKQLISNQIKAIAESNRWNVTPNP
GLLEEVNNLVEWPTAFNGGFDEKYLAIPEEVLITSMRDHQRFFFVRDQAGKLLPNFISVRNGNEEFIENVVRGNEKVLTA
RLEDAAFFYEEDQKHDINYYVDRLKKVSFHDKIGSMYEKLQRVNSIAKVIGNTLNLNQTELDDIDRATMIYKFDLVTGMV
GEFSELQGVMGEKYAQLNGENQAVAQAIREHYMPNSAEGDLPESVTGAVVALADKFDNIFSFFSAGMIPSGSNDPYALRR
HAYGIVRILNSRDWQLDLNQFKSQVKTELAENGTAFGVDVDQNFDQVLNFFNDRIKQLLDHQKISHDIVETVLTGNNHDV
TEIIEAAQVLADAKASSTFKDDIEALTRVQRIATKNEESGELNVDPQLFNNASEGELFDQIIKIEAANNLTMSQLFAKLC
ELTPAISKYFDATMVMDKDENIKRNRLNMMSRLANLILKIGDLTNVLVK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=31.8840579710145, Blast_Score=357, Evalue=1e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_PEDPA (Q03F66)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804598.1
- ProteinModelPortal:   Q03F66
- STRING:   Q03F66
- GeneID:   4417036
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1101
- NMPDR:   fig|278197.10.peg.965
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q03F66
- BioCyc:   PPEN278197:PEPE_1101-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78149; Mature: 78017

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHNYLLEIGLEEIPAHVVTPSIKQLVQKVTAFLKENRLTYDSIDHFSTPRRLAIRINGL
CCCCEEEECCHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECC
GDQQPDIEEDAKGPARKIAQDADGNWTKAAIGFTRGQGLTVDDITFKTIKGTDYVYVHKL
CCCCCCCHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEECCCCHHHHHHH
IKGKMTKEILTGLKEVVESINFPTMMKWANFDFKYVRPIRWLVSILDEEVLPFSILDVTA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCC
GRRTEGHRFLGEAVELANAEEYEAKLHDQFVIVDADERKQLISNQIKAIAESNRWNVTPN
CCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCC
PGLLEEVNNLVEWPTAFNGGFDEKYLAIPEEVLITSMRDHQRFFFVRDQAGKLLPNFISV
CCHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RNGNEEFIENVVRGNEKVLTARLEDAAFFYEEDQKHDINYYVDRLKKVSFHDKIGSMYEK
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQRVNSIAKVIGNTLNLNQTELDDIDRATMIYKFDLVTGMVGEFSELQGVMGEKYAQLNG
HHHHHHHHHHHCCCCCCCCHHHHHCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENQAVAQAIREHYMPNSAEGDLPESVTGAVVALADKFDNIFSFFSAGMIPSGSNDPYALR
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RHAYGIVRILNSRDWQLDLNQFKSQVKTELAENGTAFGVDVDQNFDQVLNFFNDRIKQLL
HHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
DHQKISHDIVETVLTGNNHDVTEIIEAAQVLADAKASSTFKDDIEALTRVQRIATKNEES
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
GELNVDPQLFNNASEGELFDQIIKIEAANNLTMSQLFAKLCELTPAISKYFDATMVMDKD
CCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHEEECCC
ENIKRNRLNMMSRLANLILKIGDLTNVLVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AHNYLLEIGLEEIPAHVVTPSIKQLVQKVTAFLKENRLTYDSIDHFSTPRRLAIRINGL
CCCEEEECCHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECC
GDQQPDIEEDAKGPARKIAQDADGNWTKAAIGFTRGQGLTVDDITFKTIKGTDYVYVHKL
CCCCCCCHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEECCCCHHHHHHH
IKGKMTKEILTGLKEVVESINFPTMMKWANFDFKYVRPIRWLVSILDEEVLPFSILDVTA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCC
GRRTEGHRFLGEAVELANAEEYEAKLHDQFVIVDADERKQLISNQIKAIAESNRWNVTPN
CCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCC
PGLLEEVNNLVEWPTAFNGGFDEKYLAIPEEVLITSMRDHQRFFFVRDQAGKLLPNFISV
CCHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RNGNEEFIENVVRGNEKVLTARLEDAAFFYEEDQKHDINYYVDRLKKVSFHDKIGSMYEK
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQRVNSIAKVIGNTLNLNQTELDDIDRATMIYKFDLVTGMVGEFSELQGVMGEKYAQLNG
HHHHHHHHHHHCCCCCCCCHHHHHCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENQAVAQAIREHYMPNSAEGDLPESVTGAVVALADKFDNIFSFFSAGMIPSGSNDPYALR
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RHAYGIVRILNSRDWQLDLNQFKSQVKTELAENGTAFGVDVDQNFDQVLNFFNDRIKQLL
HHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
DHQKISHDIVETVLTGNNHDVTEIIEAAQVLADAKASSTFKDDIEALTRVQRIATKNEES
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
GELNVDPQLFNNASEGELFDQIIKIEAANNLTMSQLFAKLCELTPAISKYFDATMVMDKD
CCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHEEECCC
ENIKRNRLNMMSRLANLILKIGDLTNVLVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA