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The map label for this gene is rpoD [H]

Identifier: 116492861

GI number: 116492861

Start: 1097323

End: 1098462

Strand: Reverse

Name: rpoD [H]

Synonym: PEPE_1099

Alternate gene names: 116492861

Gene position: 1098462-1097323 (Counterclockwise)

Preceding gene: 116492862

Following gene: 116492860

Centisome position: 59.95

GC content: 36.67

Gene sequence:

>1140_bases
ATGGCAAACAAAAAAACTGAAACAAAAAAGAAAAAGAGTGAAAGTATCATTTCTTCACCTGAGTTTAAAAAAGCTGTCAA
TCAAATCATTAAAGCTCGTAAGAGTGAAAAGAAAGTTAAATATACACAATTAACTAAAGATTTGATTACTAAGTTTAAAT
TAACTGACGAAGATGTTGATGATCTTATTCAACGCATTGAAGATGCTGGAATTAGTGTTGTCGATGAAAATGGTGATCCA
AGTGAAAAAGCTTTGAAAGCTCAAGAAATTTCTAAGGAAGAATTAGATGATACAAAGGCTCCTGCTGGAGTAAAAATTAA
TGACCCAGTTCGTATGTACCTTAAGGAAATTGGTCGTGTATCACTTCTTACTGCTGATCAAGAAGTCGAATTAGCTTTAA
GAATTGAAAAGGGTGATCCCCGTGCCCGTCAAGAATTAGCAGAAGCTAACCTTCGTTTGGTAGTATCAATTGCTAAACGT
TATGTGGGTCGTGGAATGCAATTCCTTGATTTAATTCAAGAAGGTAACATGGGCTTGATGAAAGCTGTTGAAAAATTTGA
CTACCAAAAAGGATTTAAATTTTCTACGTATGCTACATGGTGGATTCGACAAGCCATTACACGTGCGATTGCCGATCAAG
CGCGTACAATTCGAATTCCTGTTCACATGGTGGAAACCATCAATAAATTGATTCGAATTCAACGTCAATTGCTCCAAGAT
TTAGGTCGTGAACCAATGCCTGAAGAAATTGGTGCTGAAATGGATATGCCATCTGATAAAGTTCGTGAAATTCTAAAAAT
TGCTCAAGAACCAGTTTCATTAGAAACACCAATTGGTGAAGAAGATGACTCACATCTTGGTGATTTCATTGAAGATCAAG
AAGCAACTAGTCCTGCTGACCATGCTGCATACGAATTGCTAAAGGAACAACTTGAAGGTGTTCTCGATACGCTAACTGAT
CGTGAAGAAAACGTGCTACGTTTGCGCTTTGGATTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATT
TGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGATCAAAACAAC
TAAAAGATTTTCTAGATTAG

Upstream 100 bases:

>100_bases
GATTAGGTAACAAAGAATTACAACAACAACTAACTTTAAAACTTATCGCGCTATATCAAAAACAACAACAGCTTCAAAAA
TAATAGGGGGATCAGCATAA

Downstream 100 bases:

>100_bases
TTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATA
GTAGGGGTTTATTTTATGAA

Product: DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Products: NA

Alternate protein names: Sigma-42 [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MANKKTETKKKKSESIISSPEFKKAVNQIIKARKSEKKVKYTQLTKDLITKFKLTDEDVDDLIQRIEDAGISVVDENGDP
SEKALKAQEISKEELDDTKAPAGVKINDPVRMYLKEIGRVSLLTADQEVELALRIEKGDPRARQELAEANLRLVVSIAKR
YVGRGMQFLDLIQEGNMGLMKAVEKFDYQKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQD
LGREPMPEEIGAEMDMPSDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEGVLDTLTD
REENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLD

Sequences:

>Translated_379_residues
MANKKTETKKKKSESIISSPEFKKAVNQIIKARKSEKKVKYTQLTKDLITKFKLTDEDVDDLIQRIEDAGISVVDENGDP
SEKALKAQEISKEELDDTKAPAGVKINDPVRMYLKEIGRVSLLTADQEVELALRIEKGDPRARQELAEANLRLVVSIAKR
YVGRGMQFLDLIQEGNMGLMKAVEKFDYQKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQD
LGREPMPEEIGAEMDMPSDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEGVLDTLTD
REENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLD
>Mature_378_residues
ANKKTETKKKKSESIISSPEFKKAVNQIIKARKSEKKVKYTQLTKDLITKFKLTDEDVDDLIQRIEDAGISVVDENGDPS
EKALKAQEISKEELDDTKAPAGVKINDPVRMYLKEIGRVSLLTADQEVELALRIEKGDPRARQELAEANLRLVVSIAKRY
VGRGMQFLDLIQEGNMGLMKAVEKFDYQKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDL
GREPMPEEIGAEMDMPSDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEGVLDTLTDR
EENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=269, Percent_Identity=64.6840148698885, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI1789098, Length=306, Percent_Identity=43.7908496732026, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=29.7794117647059, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788231, Length=251, Percent_Identity=26.6932270916335, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43296; Mature: 43165

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKTETKKKKSESIISSPEFKKAVNQIIKARKSEKKVKYTQLTKDLITKFKLTDEDVD
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLIQRIEDAGISVVDENGDPSEKALKAQEISKEELDDTKAPAGVKINDPVRMYLKEIGRV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCE
SLLTADQEVELALRIEKGDPRARQELAEANLRLVVSIAKRYVGRGMQFLDLIQEGNMGLM
EEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
KAVEKFDYQKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHH
LGREPMPEEIGAEMDMPSDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPAD
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
HAAYELLKEQLEGVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAK
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALRKLRHPSRSKQLKDFLD
HHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
ANKKTETKKKKSESIISSPEFKKAVNQIIKARKSEKKVKYTQLTKDLITKFKLTDEDVD
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLIQRIEDAGISVVDENGDPSEKALKAQEISKEELDDTKAPAGVKINDPVRMYLKEIGRV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCE
SLLTADQEVELALRIEKGDPRARQELAEANLRLVVSIAKRYVGRGMQFLDLIQEGNMGLM
EEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
KAVEKFDYQKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHH
LGREPMPEEIGAEMDMPSDKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPAD
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
HAAYELLKEQLEGVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAK
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALRKLRHPSRSKQLKDFLD
HHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8914262; 12663927 [H]