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The map label for this gene is smpB

Identifier: 116492267

GI number: 116492267

Start: 519639

End: 520106

Strand: Direct

Name: smpB

Synonym: PEPE_0465

Alternate gene names: 116492267

Gene position: 519639-520106 (Clockwise)

Preceding gene: 116492266

Following gene: 116492271

Centisome position: 28.36

GC content: 37.82

Gene sequence:

>468_bases
ATGGCTAAGAAACCAAAGTCCAATGATAAAGCACTTGCCCAAAATAAAAAGGCGCGTCATGATTATTCCATATTAAGTAC
GATGGAAGCGGGAATGGTTTTAACGGGGACGGAAATTAAATCCATCAGAGATCGGCGAGTAACGTTGAGAGATGGATATG
TACAGATCCGGCACGGAGAGGCCATCCTCATGAACGTTCAAATTAATGAATATTTACAAGGAAATCAATTTAACCATGAT
CCCTTCCGTAACCGAAAACTTTTATTGCATAAAAAAGAAATTTTGAAGTTACAAGAAGAAACCAAAAATAAGGGAATCAC
CATCGTACCGTTGAAGATGTATCTAAAAAATGGTTTTGCCAAAGTCCTAATCGGAGTTGCCAAAGGTAAACACGAATACG
ATAAACGTGAATCATTAAAGAAGCGTGATCAACAACGGGAAATCGATCGAGCCTTAAAGAATAGATAA

Upstream 100 bases:

>100_bases
ATAAATCAGGAAATCGAAACGGAAAACCATTTTACAAAGATAAAACGGTCAAGAAGTTTTCTAAAAATACTTCTCATAAG
AAATAAGAGGAGGTTATGTT

Downstream 100 bases:

>100_bases
AAAGAGATTATCCAGCGGATAATCTCTTTTTTTGTTAGATTGATTTTTTTAAATGTTGGTAATTATGGTGAGATACAACT
TGTTCATGATCATTTTTGAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAKKPKSNDKALAQNKKARHDYSILSTMEAGMVLTGTEIKSIRDRRVTLRDGYVQIRHGEAILMNVQINEYLQGNQFNHD
PFRNRKLLLHKKEILKLQEETKNKGITIVPLKMYLKNGFAKVLIGVAKGKHEYDKRESLKKRDQQREIDRALKNR

Sequences:

>Translated_155_residues
MAKKPKSNDKALAQNKKARHDYSILSTMEAGMVLTGTEIKSIRDRRVTLRDGYVQIRHGEAILMNVQINEYLQGNQFNHD
PFRNRKLLLHKKEILKLQEETKNKGITIVPLKMYLKNGFAKVLIGVAKGKHEYDKRESLKKRDQQREIDRALKNR
>Mature_154_residues
AKKPKSNDKALAQNKKARHDYSILSTMEAGMVLTGTEIKSIRDRRVTLRDGYVQIRHGEAILMNVQINEYLQGNQFNHDP
FRNRKLLLHKKEILKLQEETKNKGITIVPLKMYLKNGFAKVLIGVAKGKHEYDKRESLKKRDQQREIDRALKNR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=154, Percent_Identity=42.8571428571429, Blast_Score=118, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PEDPA (Q03GW2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804002.1
- ProteinModelPortal:   Q03GW2
- SMR:   Q03GW2
- STRING:   Q03GW2
- GeneID:   4418362
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0465
- NMPDR:   fig|278197.10.peg.432
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q03GW2
- ProtClustDB:   PRK05422
- BioCyc:   PPEN278197:PEPE_0465-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18081; Mature: 17950

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPKSNDKALAQNKKARHDYSILSTMEAGMVLTGTEIKSIRDRRVTLRDGYVQIRHGE
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCEEEECHHHHHHHHCEEEEECCEEEEECCC
AILMNVQINEYLQGNQFNHDPFRNRKLLLHKKEILKLQEETKNKGITIVPLKMYLKNGFA
EEEEEEEEHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEHHHHHHCCHH
KVLIGVAKGKHEYDKRESLKKRDQQREIDRALKNR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKPKSNDKALAQNKKARHDYSILSTMEAGMVLTGTEIKSIRDRRVTLRDGYVQIRHGE
CCCCCCCCHHHHHCCCCHHHHHHHHHHHCCEEEECHHHHHHHHCEEEEECCEEEEECCC
AILMNVQINEYLQGNQFNHDPFRNRKLLLHKKEILKLQEETKNKGITIVPLKMYLKNGFA
EEEEEEEEHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEHHHHHHCCHH
KVLIGVAKGKHEYDKRESLKKRDQQREIDRALKNR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA