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The map label for this gene is hmp [H]

Identifier: 116249201

GI number: 116249201

Start: 582562

End: 583632

Strand: Reverse

Name: hmp [H]

Synonym: pRL120537

Alternate gene names: 116249201

Gene position: 583632-582562 (Counterclockwise)

Preceding gene: 116249202

Following gene: 116249182

Centisome position: 67.08

GC content: 62.65

Gene sequence:

>1071_bases
ATGCCTGCAGCCAGGGCCGCCCTCGACGACGTCTGGAACCCTGAAGACGACGACGCGCTCGTCTGCCTCGACGTCCAGCA
GGAGACCCACGACGTAAAGACGTTCACCTTTGCCTCCCGTGAAGGCAAGCGGTTCGCGTTCAAGGCCGGGCAGTACTTTC
TGTTCGACCTGGAACGTGATGGAGATGCTGAAAACAGGTGCTACAGCATCTCCTCCTCGCCGCATCGGACGAACGCTTTC
TCCGTGACGGTGAAGCGCGTTCCCGGCGGAAAGATCTCCAACTGGCTTCATGACACTCTAGTTCCCGGCGCGACCGTCAA
GGCGAACGGGCCGCTCGGCCACTTCGTCAGGCCCGAGACGTCGGGACGCAAGCTTCTGCTTTTGTCCGGTGGCTCCGGCA
TTACCCCCGTCATGTCCATTCTGCGGGAACTCGCCGACAGTTGCGAACCTGCCGACGTCGTCTTCATGCACGCGGGACGG
ACGCCTCAGGATCTGATCTTTCGCGACGAACTGGCCTGCATCGCCCGCAGGCTGAAAGGCCTTCGCCTCCATTTCCTCCC
GGAGACCGTGGCCGGCGAGCCGTCCTGGCCCGGTCTGACCGGTCGCATTTCGAAAGACTACATGCGGCTTGCGGTTCCCG
ATATCGCCGAGCGAACCGTGATGTGCTGCGGCCCCGCCCCGTTCATGGCCGCCGCGCGCAGCATTGCCGCCGAACTCGGT
GTGCCCGGCTCGAATTACCTGGAGGAAAGCTTCGACGCAGCAGTCGTCGACGAAGCCGAAATCCCCGCGGTTCAGGAGGC
AGCTGCCAAGGTCTTTCAGGTCACTTTCTCGAAGCAGGCGCGCAGCATCGACGTCTCCGGCGACCAGAGCGTGCTCTCAT
GCGCGAAGAAGTCAGGCGTCAGGATCCCGTCGTCCTGCGCGAATGGCGTCTGCGGCACCTGCAAGTCGAAACTTACGTCA
GGCACGGTCGACATGAACCACAATGGCGGAATTCGTCAGCGGGAAATCGACGCCGGCTTCTTCCTGCCCTGTTGCTCCAA
GCCGCTCAGCGATCTGGTCATCGAGCGCTGA

Upstream 100 bases:

>100_bases
CAGCCCGGCCCCTACTCCCGCTTTTCCGAGACGAACCTCGACAAGTTTGCCAGCTGGTACATCGATCGGATGCGCGCCCA
TGGATACTAGGAGCCCGCAG

Downstream 100 bases:

>100_bases
ACGCCGGCATAACAGGGACTTACTTAAATGTTGCGAGATTGCGGGCTCAGTGCGCTCTCGAAGCGGCACGATGCTGCGAT
GGCGTTTGCCCGAATGTGCG

Product: putative FAD/NAD/ferredoxin protein

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPAARAALDDVWNPEDDDALVCLDVQQETHDVKTFTFASREGKRFAFKAGQYFLFDLERDGDAENRCYSISSSPHRTNAF
SVTVKRVPGGKISNWLHDTLVPGATVKANGPLGHFVRPETSGRKLLLLSGGSGITPVMSILRELADSCEPADVVFMHAGR
TPQDLIFRDELACIARRLKGLRLHFLPETVAGEPSWPGLTGRISKDYMRLAVPDIAERTVMCCGPAPFMAAARSIAAELG
VPGSNYLEESFDAAVVDEAEIPAVQEAAAKVFQVTFSKQARSIDVSGDQSVLSCAKKSGVRIPSSCANGVCGTCKSKLTS
GTVDMNHNGGIRQREIDAGFFLPCCSKPLSDLVIER

Sequences:

>Translated_356_residues
MPAARAALDDVWNPEDDDALVCLDVQQETHDVKTFTFASREGKRFAFKAGQYFLFDLERDGDAENRCYSISSSPHRTNAF
SVTVKRVPGGKISNWLHDTLVPGATVKANGPLGHFVRPETSGRKLLLLSGGSGITPVMSILRELADSCEPADVVFMHAGR
TPQDLIFRDELACIARRLKGLRLHFLPETVAGEPSWPGLTGRISKDYMRLAVPDIAERTVMCCGPAPFMAAARSIAAELG
VPGSNYLEESFDAAVVDEAEIPAVQEAAAKVFQVTFSKQARSIDVSGDQSVLSCAKKSGVRIPSSCANGVCGTCKSKLTS
GTVDMNHNGGIRQREIDAGFFLPCCSKPLSDLVIER
>Mature_355_residues
PAARAALDDVWNPEDDDALVCLDVQQETHDVKTFTFASREGKRFAFKAGQYFLFDLERDGDAENRCYSISSSPHRTNAFS
VTVKRVPGGKISNWLHDTLVPGATVKANGPLGHFVRPETSGRKLLLLSGGSGITPVMSILRELADSCEPADVVFMHAGRT
PQDLIFRDELACIARRLKGLRLHFLPETVAGEPSWPGLTGRISKDYMRLAVPDIAERTVMCCGPAPFMAAARSIAAELGV
PGSNYLEESFDAAVVDEAEIPAVQEAAAKVFQVTFSKQARSIDVSGDQSVLSCAKKSGVRIPSSCANGVCGTCKSKLTSG
TVDMNHNGGIRQREIDAGFFLPCCSKPLSDLVIER

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=330, Percent_Identity=31.5151515151515, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787658, Length=354, Percent_Identity=30.7909604519774, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788104, Length=324, Percent_Identity=26.5432098765432, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788903, Length=219, Percent_Identity=26.9406392694064, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=196, Percent_Identity=32.6530612244898, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323552, Length=200, Percent_Identity=25.5, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322699, Length=223, Percent_Identity=26.0089686098655, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322146, Length=261, Percent_Identity=22.6053639846743, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38437; Mature: 38306

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAARAALDDVWNPEDDDALVCLDVQQETHDVKTFTFASREGKRFAFKAGQYFLFDLERD
CCCHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCC
GDAENRCYSISSSPHRTNAFSVTVKRVPGGKISNWLHDTLVPGATVKANGPLGHFVRPET
CCCCCCEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
SGRKLLLLSGGSGITPVMSILRELADSCEPADVVFMHAGRTPQDLIFRDELACIARRLKG
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LRLHFLPETVAGEPSWPGLTGRISKDYMRLAVPDIAERTVMCCGPAPFMAAARSIAAELG
CEEEECCHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHC
VPGSNYLEESFDAAVVDEAEIPAVQEAAAKVFQVTFSKQARSIDVSGDQSVLSCAKKSGV
CCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCC
RIPSSCANGVCGTCKSKLTSGTVDMNHNGGIRQREIDAGFFLPCCSKPLSDLVIER
CCCHHHCCCCCHHHHHHHCCCCEEECCCCCCEEEECCCCEECHHHCCCHHHHHCCC
>Mature Secondary Structure 
PAARAALDDVWNPEDDDALVCLDVQQETHDVKTFTFASREGKRFAFKAGQYFLFDLERD
CCHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCC
GDAENRCYSISSSPHRTNAFSVTVKRVPGGKISNWLHDTLVPGATVKANGPLGHFVRPET
CCCCCCEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
SGRKLLLLSGGSGITPVMSILRELADSCEPADVVFMHAGRTPQDLIFRDELACIARRLKG
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LRLHFLPETVAGEPSWPGLTGRISKDYMRLAVPDIAERTVMCCGPAPFMAAARSIAAELG
CEEEECCHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHC
VPGSNYLEESFDAAVVDEAEIPAVQEAAAKVFQVTFSKQARSIDVSGDQSVLSCAKKSGV
CCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCC
RIPSSCANGVCGTCKSKLTSGTVDMNHNGGIRQREIDAGFFLPCCSKPLSDLVIER
CCCHHHCCCCCHHHHHHHCCCCEEECCCCCCEEEECCCCEECHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]