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The map label for this gene is trpS [H]

Identifier: 114800520

GI number: 114800520

Start: 514854

End: 515888

Strand: Direct

Name: trpS [H]

Synonym: HNE_0520

Alternate gene names: 114800520

Gene position: 514854-515888 (Clockwise)

Preceding gene: 114797264

Following gene: 114799560

Centisome position: 13.9

GC content: 62.8

Gene sequence:

>1035_bases
ATGACTGACACACCCCCCGCCTATACAGGCCCGCAACGCGTTTTCTCCGGCGTCCAGCCGACCGGCAATCTTCACCTCGG
GAATTATCTCGGGGCGCTGAAGAAATTCGCCGACCTGCAACGCGACGGCCATGACTGCATCTTCGCCGTCGTGGACCTTC
ACGCCATCACCATGCCCATGGAGAAGGGGGCCCTGATCTCGCACACCCGCCAGATCGCCGCCGCCTTCATCGCGGCCGGT
GTTGACCCGTCCAAATCCATCATCTTTGCCCAGTCTTCGGTACTGGCGCATGTGGAGCTGGCCTGGATCTTCAACTGCGT
CGCGCGGATGGGCTGGGTCGAGCGGATGACCCAGTTCAAGGACAAAGCCGGCAAGGACGCCGAGCGCGCCTCCGTGGGGC
TCTTCACCTATCCGGTGCTCCAGGCCGCTGACATCCTCGCCTACAAGGCGACGCATGTGCCCGTGGGCGAAGACCAGAAA
CAGCATCTCGAACTCAGCCGCGACATCGCCGAGCGCTTCAACCGCGAATACGATGTGCCGGGCTTCTTCCCGCAGCCTGA
ACCCATGATCAAAGGCCCCGGTGCCCGGATCATGAGCCTCAAGGACGGCACGAAGAAAATGTCGAAATCCGACCCGTCGG
ACCTCTCGCGCATCAACCTGGTCGACGACGCCGATACCATCGCGAAGAAGATCAAGAAGGCCACCACCGATGCCGGCGTC
ATTCCGGGTACGGTCAAAGAACTTGAGGGCCGTCCCGAAGTCGAAAACCTCGTCGGCATTTATGCTGCCCTCTCCGGGCG
CACTGCGGAAGACGTTCTGGCCGAATTTGAAGGCAAGGGTTTTGGCGCTTTCAAGCCCGCGCTGGCCGATGTCACGGTCG
CCCATCTGGCGCCGATCACCGCGAAATTCCGCGACATCCTGGACGATCAGACGGGCCTGGACCGCGTCCTCGCAGACGGC
GCCGCGCGCGCCAATGAGGTTGCTGCGCCGGTCATGGATGATGTCCGCCGGATCGTCGGCTTCTGGCGCGGCTGA

Upstream 100 bases:

>100_bases
TTTATCTATACCTTTGCTGCCTTCCTGACCGGCGCCGTCCGCCTCAGCGACATCCGGCAGGCTTTGCGGCGCTGATCAAA
CGCGCTAAAGGCGCCCCACC

Downstream 100 bases:

>100_bases
CCTTCAGCACCGCTTTCTGACGTCTTGAAAGGCAGCGCCGCCTTAAAAGCGCCCCACGCGCTGCGCATACTTTTCGGCTG
TGTGGGGAATCACTCCATCC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTDTPPAYTGPQRVFSGVQPTGNLHLGNYLGALKKFADLQRDGHDCIFAVVDLHAITMPMEKGALISHTRQIAAAFIAAG
VDPSKSIIFAQSSVLAHVELAWIFNCVARMGWVERMTQFKDKAGKDAERASVGLFTYPVLQAADILAYKATHVPVGEDQK
QHLELSRDIAERFNREYDVPGFFPQPEPMIKGPGARIMSLKDGTKKMSKSDPSDLSRINLVDDADTIAKKIKKATTDAGV
IPGTVKELEGRPEVENLVGIYAALSGRTAEDVLAEFEGKGFGAFKPALADVTVAHLAPITAKFRDILDDQTGLDRVLADG
AARANEVAAPVMDDVRRIVGFWRG

Sequences:

>Translated_344_residues
MTDTPPAYTGPQRVFSGVQPTGNLHLGNYLGALKKFADLQRDGHDCIFAVVDLHAITMPMEKGALISHTRQIAAAFIAAG
VDPSKSIIFAQSSVLAHVELAWIFNCVARMGWVERMTQFKDKAGKDAERASVGLFTYPVLQAADILAYKATHVPVGEDQK
QHLELSRDIAERFNREYDVPGFFPQPEPMIKGPGARIMSLKDGTKKMSKSDPSDLSRINLVDDADTIAKKIKKATTDAGV
IPGTVKELEGRPEVENLVGIYAALSGRTAEDVLAEFEGKGFGAFKPALADVTVAHLAPITAKFRDILDDQTGLDRVLADG
AARANEVAAPVMDDVRRIVGFWRG
>Mature_343_residues
TDTPPAYTGPQRVFSGVQPTGNLHLGNYLGALKKFADLQRDGHDCIFAVVDLHAITMPMEKGALISHTRQIAAAFIAAGV
DPSKSIIFAQSSVLAHVELAWIFNCVARMGWVERMTQFKDKAGKDAERASVGLFTYPVLQAADILAYKATHVPVGEDQKQ
HLELSRDIAERFNREYDVPGFFPQPEPMIKGPGARIMSLKDGTKKMSKSDPSDLSRINLVDDADTIAKKIKKATTDAGVI
PGTVKELEGRPEVENLVGIYAALSGRTAEDVLAEFEGKGFGAFKPALADVTVAHLAPITAKFRDILDDQTGLDRVLADGA
ARANEVAAPVMDDVRRIVGFWRG

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=330, Percent_Identity=41.8181818181818, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=49.1620111731844, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=44.5121951219512, Blast_Score=284, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=339, Percent_Identity=37.4631268436578, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71982793, Length=339, Percent_Identity=37.4631268436578, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6320474, Length=346, Percent_Identity=41.0404624277457, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24666151, Length=336, Percent_Identity=39.2857142857143, Blast_Score=240, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37299; Mature: 37168

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPPAYTGPQRVFSGVQPTGNLHLGNYLGALKKFADLQRDGHDCIFAVVDLHAITMPM
CCCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EKGALISHTRQIAAAFIAAGVDPSKSIIFAQSSVLAHVELAWIFNCVARMGWVERMTQFK
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAGKDAERASVGLFTYPVLQAADILAYKATHVPVGEDQKQHLELSRDIAERFNREYDVP
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GFFPQPEPMIKGPGARIMSLKDGTKKMSKSDPSDLSRINLVDDADTIAKKIKKATTDAGV
CCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCC
IPGTVKELEGRPEVENLVGIYAALSGRTAEDVLAEFEGKGFGAFKPALADVTVAHLAPIT
CCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AKFRDILDDQTGLDRVLADGAARANEVAAPVMDDVRRIVGFWRG
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDTPPAYTGPQRVFSGVQPTGNLHLGNYLGALKKFADLQRDGHDCIFAVVDLHAITMPM
CCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EKGALISHTRQIAAAFIAAGVDPSKSIIFAQSSVLAHVELAWIFNCVARMGWVERMTQFK
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKAGKDAERASVGLFTYPVLQAADILAYKATHVPVGEDQKQHLELSRDIAERFNREYDVP
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GFFPQPEPMIKGPGARIMSLKDGTKKMSKSDPSDLSRINLVDDADTIAKKIKKATTDAGV
CCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCC
IPGTVKELEGRPEVENLVGIYAALSGRTAEDVLAEFEGKGFGAFKPALADVTVAHLAPIT
CCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AKFRDILDDQTGLDRVLADGAARANEVAAPVMDDVRRIVGFWRG
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]