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The map label for this gene is capD [H]

Identifier: 114799559

GI number: 114799559

Start: 1030514

End: 1032376

Strand: Reverse

Name: capD [H]

Synonym: HNE_1021

Alternate gene names: 114799559

Gene position: 1032376-1030514 (Counterclockwise)

Preceding gene: 114797510

Following gene: 114797405

Centisome position: 27.86

GC content: 65.11

Gene sequence:

>1863_bases
ATGGCCCTGACACTCACGTTTGACATCGCCATGGCGGGCGTGGCGATGACGGCGGCCCATCTTGATACACATCTGAGCCA
GGAATCTGGCGAGCTGTTCAACTATCAGAGCTGGTTGCTTGCGAGCGGCGCCTTCATGGTTGCAGCCGCAGTGGCGCTGG
TACTGGGCGGCATCCACCGTCAGGTGTGGCGCCATATGGGCGCGCCCGACGCCTGGCGCCTTGTACAGATCATCGCGCTG
ACCGTTCTGTTTTATCTGCCGGTGATGGTGACGTTGAACGGCGCCCTGTCCTCTCCGGTTCCGACCCTTCTGCTCGCCAT
CGGCTTCTGGACCGTGGCGCTATATGGTGGCCGGATGGTGGCGCGCTGGCGCTCGACCCAGCGCCCGATGCAGATTTTCC
AGCAATTGCCCAAGGATGGCCAACCGATCCTGCTGCTCGGCGACCAGCTGAGCTGGATCGATGTGCTGCGCCGCCTTGAG
GGCCGCGATTCGGGCCAGGCGGTCCGCGTACTCGGTCTGGTTGAAATGGACGCGCACCAGCCCGGCCGCGCGGTTCGCGG
CGTGCCGATTCTCGGCAGCCTCAAGGAACTCAGCGACATCATCGAAATCATGGTGCTGCGCTATGGCGAGACGCCCTGGA
TCGCGCTGACCGGCCCGGCCCGAACGCCCGCCGTGATGCTGCAGGTTCTGGAAGTCACCTCCCGCCACGGCGCCGAGATC
ATGGCGCTGGGCCATGACGCGGCCGCGCAGGTGCTCGAGCGGCTGCGCCCGGCCGATCTGCTGGCCCGGCCCGAACGCCA
CCTCGATATGGAGCCTGTGCGCAAGATCCTGACCGGCGCGCGGGTGCTGGTTACCGGCGGCGGCGGGTCCATCGGGTCCG
AACTCGTGCGCCAGGTCGCCCAGGAAAGCCCGGCCGAGCTGACGATCATCGATTTCTCCGAATACAATCTCTACCAGATC
GAGATGGAGCTGAAGCGCGACTGGCCCGCGCTCAAGATCAGCTGCCATCTGGGCGACATTCGCGATGAAGCGCGGATGCT
GGACATCTTCACCGGCGCCCGGCCCGATACCGTGATCCACGCCGCGGCCCTGAAACATGTGCCCCTGATGGAGCGCCATG
CCTGCGAGGCGATCCTCACCAATGTGTCCGGCGCGCGGATTGCGGCCCAGTCCGCGGTAGCGGCGGGCGCCAAATGCTTC
GTGTTCATCTCAACCGACAAGGCCGTCGATCCTGACAATGTCATGGGCGCCACCAAACGCCTCGCCGAGCTGTGCATTGG
CCGCATCCTGGCCGATTCCGGCATGGCCGGCGCCATGGTGCGCTTCGGTAATGTGCTCGGCTCGTCCGGCTCGGTTGTGC
CCTTGTTCGAGCGCCAGATTGCGCAGGGCGGCCCGGTGACCATTACCGATCCCGACGCAACGCGCTTCTTCATGACCATC
GAGGAAGCCTCGGCGCTGGTCCTTCAGGCCGCCTCGCTGCAACAGACGCGCGGCGAAGCGGGGCTCTATGTGCTCGACAT
GGGCGACCCGATCCCGATCCGCCAGCTGGCCGAAACGATGATCCGCCTCAAGGGCAAGGTGCCCTATGTCGACATCGCCA
TCGAGACGATGGGCCTGCGCGAAGGCGAAAAGCTGCACGAAGAACTGACCTATCCGCATGAGGCCCTGCAGACGACGCCG
ATCAGCGGTGTCCACCGGGTCAGCTATGAGCCGATCCAGAACGAGCTTTTCCAGAAGCAGCTGATGCAACTGATCGAGAC
CGCCCGGAACCATCAGCCTGCGGAGGCTGTGCGCCTGCTCGGCGTGCTGGTTCCCGAATATGGCTCAGAGCGCGCCGAGA
TGCTCAACCGCCGCCTGGCCTGA

Upstream 100 bases:

>100_bases
TTTTTGTGACCCAAAGTGGCACTACACGGGTGGTTCGTTCTGTACGAACGAGTGTGAGGGAGTACCAGACGTGACACCCA
GAGACGCCGCACGGCGTGCC

Downstream 100 bases:

>100_bases
GGCTGCCCCAACAGACCCGCATAAAAAAGGGCGCCCGCGAAGGCGCCCTTTTCATTGCTGCGGAAGACCGGATCAGAACT
TGCGGTTCAGGGCGATCCGG

Product: polysaccharide biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MALTLTFDIAMAGVAMTAAHLDTHLSQESGELFNYQSWLLASGAFMVAAAVALVLGGIHRQVWRHMGAPDAWRLVQIIAL
TVLFYLPVMVTLNGALSSPVPTLLLAIGFWTVALYGGRMVARWRSTQRPMQIFQQLPKDGQPILLLGDQLSWIDVLRRLE
GRDSGQAVRVLGLVEMDAHQPGRAVRGVPILGSLKELSDIIEIMVLRYGETPWIALTGPARTPAVMLQVLEVTSRHGAEI
MALGHDAAAQVLERLRPADLLARPERHLDMEPVRKILTGARVLVTGGGGSIGSELVRQVAQESPAELTIIDFSEYNLYQI
EMELKRDWPALKISCHLGDIRDEARMLDIFTGARPDTVIHAAALKHVPLMERHACEAILTNVSGARIAAQSAVAAGAKCF
VFISTDKAVDPDNVMGATKRLAELCIGRILADSGMAGAMVRFGNVLGSSGSVVPLFERQIAQGGPVTITDPDATRFFMTI
EEASALVLQAASLQQTRGEAGLYVLDMGDPIPIRQLAETMIRLKGKVPYVDIAIETMGLREGEKLHEELTYPHEALQTTP
ISGVHRVSYEPIQNELFQKQLMQLIETARNHQPAEAVRLLGVLVPEYGSERAEMLNRRLA

Sequences:

>Translated_620_residues
MALTLTFDIAMAGVAMTAAHLDTHLSQESGELFNYQSWLLASGAFMVAAAVALVLGGIHRQVWRHMGAPDAWRLVQIIAL
TVLFYLPVMVTLNGALSSPVPTLLLAIGFWTVALYGGRMVARWRSTQRPMQIFQQLPKDGQPILLLGDQLSWIDVLRRLE
GRDSGQAVRVLGLVEMDAHQPGRAVRGVPILGSLKELSDIIEIMVLRYGETPWIALTGPARTPAVMLQVLEVTSRHGAEI
MALGHDAAAQVLERLRPADLLARPERHLDMEPVRKILTGARVLVTGGGGSIGSELVRQVAQESPAELTIIDFSEYNLYQI
EMELKRDWPALKISCHLGDIRDEARMLDIFTGARPDTVIHAAALKHVPLMERHACEAILTNVSGARIAAQSAVAAGAKCF
VFISTDKAVDPDNVMGATKRLAELCIGRILADSGMAGAMVRFGNVLGSSGSVVPLFERQIAQGGPVTITDPDATRFFMTI
EEASALVLQAASLQQTRGEAGLYVLDMGDPIPIRQLAETMIRLKGKVPYVDIAIETMGLREGEKLHEELTYPHEALQTTP
ISGVHRVSYEPIQNELFQKQLMQLIETARNHQPAEAVRLLGVLVPEYGSERAEMLNRRLA
>Mature_619_residues
ALTLTFDIAMAGVAMTAAHLDTHLSQESGELFNYQSWLLASGAFMVAAAVALVLGGIHRQVWRHMGAPDAWRLVQIIALT
VLFYLPVMVTLNGALSSPVPTLLLAIGFWTVALYGGRMVARWRSTQRPMQIFQQLPKDGQPILLLGDQLSWIDVLRRLEG
RDSGQAVRVLGLVEMDAHQPGRAVRGVPILGSLKELSDIIEIMVLRYGETPWIALTGPARTPAVMLQVLEVTSRHGAEIM
ALGHDAAAQVLERLRPADLLARPERHLDMEPVRKILTGARVLVTGGGGSIGSELVRQVAQESPAELTIIDFSEYNLYQIE
MELKRDWPALKISCHLGDIRDEARMLDIFTGARPDTVIHAAALKHVPLMERHACEAILTNVSGARIAAQSAVAAGAKCFV
FISTDKAVDPDNVMGATKRLAELCIGRILADSGMAGAMVRFGNVLGSSGSVVPLFERQIAQGGPVTITDPDATRFFMTIE
EASALVLQAASLQQTRGEAGLYVLDMGDPIPIRQLAETMIRLKGKVPYVDIAIETMGLREGEKLHEELTYPHEALQTTPI
SGVHRVSYEPIQNELFQKQLMQLIETARNHQPAEAVRLLGVLVPEYGSERAEMLNRRLA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 67734; Mature: 67603

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLTFDIAMAGVAMTAAHLDTHLSQESGELFNYQSWLLASGAFMVAAAVALVLGGIHR
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHCCHHHHHHHHHHHHHHHHH
QVWRHMGAPDAWRLVQIIALTVLFYLPVMVTLNGALSSPVPTLLLAIGFWTVALYGGRMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCE
ARWRSTQRPMQIFQQLPKDGQPILLLGDQLSWIDVLRRLEGRDSGQAVRVLGLVEMDAHQ
EHHHCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
PGRAVRGVPILGSLKELSDIIEIMVLRYGETPWIALTGPARTPAVMLQVLEVTSRHGAEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEE
MALGHDAAAQVLERLRPADLLARPERHLDMEPVRKILTGARVLVTGGGGSIGSELVRQVA
EEECCHHHHHHHHHCCCHHHHCCCHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QESPAELTIIDFSEYNLYQIEMELKRDWPALKISCHLGDIRDEARMLDIFTGARPDTVIH
HCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHH
AAALKHVPLMERHACEAILTNVSGARIAAQSAVAAGAKCFVFISTDKAVDPDNVMGATKR
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHH
LAELCIGRILADSGMAGAMVRFGNVLGSSGSVVPLFERQIAQGGPVTITDPDATRFFMTI
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCEEEECCCCCEEEEEH
EEASALVLQAASLQQTRGEAGLYVLDMGDPIPIRQLAETMIRLKGKVPYVDIAIETMGLR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
EGEKLHEELTYPHEALQTTPISGVHRVSYEPIQNELFQKQLMQLIETARNHQPAEAVRLL
CHHHHHHHHCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVLVPEYGSERAEMLNRRLA
HHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ALTLTFDIAMAGVAMTAAHLDTHLSQESGELFNYQSWLLASGAFMVAAAVALVLGGIHR
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHCCHHHHHHHHHHHHHHHHH
QVWRHMGAPDAWRLVQIIALTVLFYLPVMVTLNGALSSPVPTLLLAIGFWTVALYGGRMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCE
ARWRSTQRPMQIFQQLPKDGQPILLLGDQLSWIDVLRRLEGRDSGQAVRVLGLVEMDAHQ
EHHHCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
PGRAVRGVPILGSLKELSDIIEIMVLRYGETPWIALTGPARTPAVMLQVLEVTSRHGAEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEE
MALGHDAAAQVLERLRPADLLARPERHLDMEPVRKILTGARVLVTGGGGSIGSELVRQVA
EEECCHHHHHHHHHCCCHHHHCCCHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QESPAELTIIDFSEYNLYQIEMELKRDWPALKISCHLGDIRDEARMLDIFTGARPDTVIH
HCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHH
AAALKHVPLMERHACEAILTNVSGARIAAQSAVAAGAKCFVFISTDKAVDPDNVMGATKR
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHH
LAELCIGRILADSGMAGAMVRFGNVLGSSGSVVPLFERQIAQGGPVTITDPDATRFFMTI
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCEEEECCCCCEEEEEH
EEASALVLQAASLQQTRGEAGLYVLDMGDPIPIRQLAETMIRLKGKVPYVDIAIETMGLR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
EGEKLHEELTYPHEALQTTPISGVHRVSYEPIQNELFQKQLMQLIETARNHQPAEAVRLL
CHHHHHHHHCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVLVPEYGSERAEMLNRRLA
HHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]