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The map label for this gene is tilS

Identifier: 114799417

GI number: 114799417

Start: 138805

End: 139908

Strand: Direct

Name: tilS

Synonym: HNE_0157

Alternate gene names: 114799417

Gene position: 138805-139908 (Clockwise)

Preceding gene: 114800143

Following gene: 114797326

Centisome position: 3.75

GC content: 65.49

Gene sequence:

>1104_bases
ATGCTCGGGCTAGGTCCGCAACAGGCGGCCTTTGAGGCAGCGCTCGGCGCGCTGCCTGATCGGTTGCTGGATACCCCCGT
TGGTGTGGCGGTTTCCGGGGGCAGTGATTCCCTCGCGCTTCTGATCCTGGCGCATCGCTGGGCCGCCCGGCGCGGGCGGG
TGTTGCGGGCGCTGACGGTCGATCATGGTCTGCGCCCGGAGTCGCGGGCCGAGGCCGAGGCCGTTGGTCGTCTCTGCGCG
GACATGGGTATTGAGCATGACATCCTGCGTCTTGAAGGCGCCGCGCCACGCCAATCGGCACTGCGGCGCGGACGCCATGC
CGCGCTCGCCAGGACAATCGCGGACAAATCTGGACACTTGCTGCTGACGGGGCATACGGCAGATGATCAGGCCGAGACCT
TCCTCATGCGCGCCCGGCAGGGGTCGGGCTGGTACGGGCTTGCCGGGATGCGCCCGCTTTCGCTCTCCCCGGTGTGGCCC
GAAGGGGAGGGGGTGTTCATTGCCCGGCCACTGCTCGGCATCCGCCGGGAGGCGCTGCGCGCCTTCCTGAGGGACGAGGG
GCTGGGTTGGGCAGACGACCCCTCCAACGACAATCCGGCCTTCGAGCGCGTGCGCATGCGCCGCCTTTTGTGTCCGGAAA
TGTCCCGGAATGTCCCGGTTTTGTCATTGGTTGACCGGTTTCAGACATTAAGAATGATTGAGGATGGCGCCATTTGGCGT
TGGATGACCGCGAATGTCCGGGCCGGTGAGGCGGGAATCCATGTCGCATCCTTTGCCGGGCACCCTCCCGAAAGGGCCGC
AAGAGCGTTGGGTATGCTCCTTCAGATCGCCGCAGGACGCGAGATGCCTCCCAGAGGGGAAAGCCTCCGCCGCCTCGCCG
AAGAGATTGTTTCAAAAGGAGATTTCCGGGGCGCCACGCTCGGCGGATGCCGGATCACGGCCCGCCGCACGCATATCTGT
CTCAAGCCCGAATGCGGCCCGCTCCCCCCCGGCACTGCGGCGCGCCTCGCCGCAACCCAGGCCATCCTTTCCGGCAACCC
GAATGAAATTGCCGCATCAGCAGGAAAGGAGTCGTTTTTAGAGGATTTGGTGCCTATCTTCTAA

Upstream 100 bases:

>100_bases
CGGCAAAGGCCTGCCAGGCACTCGATACGCTGCCCAAGCGCTATCCGAATGCCTCCAAGGTGGTCCGTGACCTGGCAGCG
GTCGAGCGCACGCGCTCCGG

Downstream 100 bases:

>100_bases
ACTCGTTCCCGTATGGTGGATCGAGTAGTTTCTGACGGAACCAACGCTATGAATGTCCGGAATTTGGCGATCTGGGGCGC
GCTCGCCCTGCTTGTGATCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLGLGPQQAAFEAALGALPDRLLDTPVGVAVSGGSDSLALLILAHRWAARRGRVLRALTVDHGLRPESRAEAEAVGRLCA
DMGIEHDILRLEGAAPRQSALRRGRHAALARTIADKSGHLLLTGHTADDQAETFLMRARQGSGWYGLAGMRPLSLSPVWP
EGEGVFIARPLLGIRREALRAFLRDEGLGWADDPSNDNPAFERVRMRRLLCPEMSRNVPVLSLVDRFQTLRMIEDGAIWR
WMTANVRAGEAGIHVASFAGHPPERAARALGMLLQIAAGREMPPRGESLRRLAEEIVSKGDFRGATLGGCRITARRTHIC
LKPECGPLPPGTAARLAATQAILSGNPNEIAASAGKESFLEDLVPIF

Sequences:

>Translated_367_residues
MLGLGPQQAAFEAALGALPDRLLDTPVGVAVSGGSDSLALLILAHRWAARRGRVLRALTVDHGLRPESRAEAEAVGRLCA
DMGIEHDILRLEGAAPRQSALRRGRHAALARTIADKSGHLLLTGHTADDQAETFLMRARQGSGWYGLAGMRPLSLSPVWP
EGEGVFIARPLLGIRREALRAFLRDEGLGWADDPSNDNPAFERVRMRRLLCPEMSRNVPVLSLVDRFQTLRMIEDGAIWR
WMTANVRAGEAGIHVASFAGHPPERAARALGMLLQIAAGREMPPRGESLRRLAEEIVSKGDFRGATLGGCRITARRTHIC
LKPECGPLPPGTAARLAATQAILSGNPNEIAASAGKESFLEDLVPIF
>Mature_367_residues
MLGLGPQQAAFEAALGALPDRLLDTPVGVAVSGGSDSLALLILAHRWAARRGRVLRALTVDHGLRPESRAEAEAVGRLCA
DMGIEHDILRLEGAAPRQSALRRGRHAALARTIADKSGHLLLTGHTADDQAETFLMRARQGSGWYGLAGMRPLSLSPVWP
EGEGVFIARPLLGIRREALRAFLRDEGLGWADDPSNDNPAFERVRMRRLLCPEMSRNVPVLSLVDRFQTLRMIEDGAIWR
WMTANVRAGEAGIHVASFAGHPPERAARALGMLLQIAAGREMPPRGESLRRLAEEIVSKGDFRGATLGGCRITARRTHIC
LKPECGPLPPGTAARLAATQAILSGNPNEIAASAGKESFLEDLVPIF

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=195, Percent_Identity=32.3076923076923, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_HYPNA (Q0C5V2)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758891.1
- ProteinModelPortal:   Q0C5V2
- SMR:   Q0C5V2
- STRING:   Q0C5V2
- GeneID:   4289554
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0157
- NMPDR:   fig|228405.5.peg.155
- TIGR:   HNE_0157
- eggNOG:   COG0037
- OMA:   NPDSAAW
- BioCyc:   HNEP81032:HNE_0157-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 39592; Mature: 39592

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLGPQQAAFEAALGALPDRLLDTPVGVAVSGGSDSLALLILAHRWAARRGRVLRALTV
CCCCCCHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEC
DHGLRPESRAEAEAVGRLCADMGIEHDILRLEGAAPRQSALRRGRHAALARTIADKSGHL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCE
LLTGHTADDQAETFLMRARQGSGWYGLAGMRPLSLSPVWPEGEGVFIARPLLGIRREALR
EEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
AFLRDEGLGWADDPSNDNPAFERVRMRRLLCPEMSRNVPVLSLVDRFQTLRMIEDGAIWR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCCEEE
WMTANVRAGEAGIHVASFAGHPPERAARALGMLLQIAAGREMPPRGESLRRLAEEIVSKG
EEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
DFRGATLGGCRITARRTHICLKPECGPLPPGTAARLAATQAILSGNPNEIAASAGKESFL
CCCCCEECCEEEEEECEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHH
EDLVPIF
HHHHCCC
>Mature Secondary Structure
MLGLGPQQAAFEAALGALPDRLLDTPVGVAVSGGSDSLALLILAHRWAARRGRVLRALTV
CCCCCCHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEC
DHGLRPESRAEAEAVGRLCADMGIEHDILRLEGAAPRQSALRRGRHAALARTIADKSGHL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCE
LLTGHTADDQAETFLMRARQGSGWYGLAGMRPLSLSPVWPEGEGVFIARPLLGIRREALR
EEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
AFLRDEGLGWADDPSNDNPAFERVRMRRLLCPEMSRNVPVLSLVDRFQTLRMIEDGAIWR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCCEEE
WMTANVRAGEAGIHVASFAGHPPERAARALGMLLQIAAGREMPPRGESLRRLAEEIVSKG
EEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
DFRGATLGGCRITARRTHICLKPECGPLPPGTAARLAATQAILSGNPNEIAASAGKESFL
CCCCCEECCEEEEEECEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHH
EDLVPIF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA