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The map label for this gene is hisS [H]

Identifier: 114797537

GI number: 114797537

Start: 573266

End: 574759

Strand: Direct

Name: hisS [H]

Synonym: HNE_0577

Alternate gene names: 114797537

Gene position: 573266-574759 (Clockwise)

Preceding gene: 114797100

Following gene: 114798501

Centisome position: 15.47

GC content: 66.93

Gene sequence:

>1494_bases
ATGAGCGACAAGGACAACCAGCCCCTCAGCGGCCCCCTTTCCGGCAAGGATCTCGCCCGGAAACCCCGCGGTTTTCCCGA
CAAGCGCGAGAGCCTGATCCACGCCCAGGCGCGGCTGGTGGAAACGGTGACCGGGGTTTACCGGCAATGGGGCTTTGAGG
CGCTTGATACTGGCGCCTTTGAGTATGCCGACGCGCTCGGCAAGTTCCTGCCCGATGCCGACCGGCCCAATGCCGGTGTG
TTCTCCATGCAGGATGATGACGCGCAGTGGATGGCGCTGCGCTATGATCTGACCGCGCCGCTCGCCCGCTTCGTGGCCGA
GCAGGGGCAGGGCCTTGGCCGCCCCTTCCGCCGCTATGCCGCTGGCCCCGTCTGGCGCAACGAAAAGCCCGGCCCCGGGC
GCTTCCGCGAATTCTGGCAGTGCGACGCCGATACCGTCGGCGCGCCCGGCTTTCACGCCGATGCCGAGATGATCGCGATG
GGGGCGGAGGCTCTGCGCGCCATCGGCATGAAGCCCGGCGAGTTTGTGATCCGCGTCAACACGCGGCGCCTGCTCAACGG
CGTGCTCGACGGCGTGGGCGCGACCGGGGCAGACATCCGCCTCGCGATCCTGCGCGCGCTCGACAAGCTCGACCGGCTGG
GCGCTTCGGGCGTTGCTGACCTGCTGGGCGCAGGCCGCAAGGATGAGAGCGGCGACTTTACCAAGGGCGCTGGGCTCGGG
CCGGAAGAGATCAAGCGCGTGCTCGCCTTTGCCGAAGCCGGCGCCGACACGCGCGCCATGACGCTGGCCAATCTCGCCAA
GGCCACCGGCAGCACCGAGGAAGGCAAGGCCGGCCTCGGCGAGCTGGAAGCCATCGCGCTGGCCCTCGAAGCGCTCGGTG
CGCCGGAGGATGACGTAAAGATCGACCCCTCCGTTGTCAGAGGTCTGGAATACTATACCGGCCCGGTGTTTGAGGCAGAG
CTGCTGCGCGAAGTGACCGGGGAAGACGGCAAGACCTATCGCATCGGCTCCATCGGCGGGGGCGGGCGGTATGATGATCT
CGTTGCGCGCTTTACCGGCGAGACCGTGCCTGCGACGGGCTTCTCCATCGGCATCTCGCGGCTCGCGGCGGCGCTGGCCA
TAATGGGGGAGGGCGGAAAGCTCGACGGCCCGGTCGTGGTGCTTAATCTGGACAAGGACAATCCGGGTGTTGCGCTCGGT
CTCGCCGCAGAACTTCGCCGGGGCGGCATTCGCGCCGAAGCCTATATGGGCGCCTCGGGCATGCGTCCGCAGATGAAATA
CGCCGACCGGCGCAATTCGCCGGCGGTTGTCATTGTCGGCGGCGATGAGCTTGAAAAAGGCACGGTCACGATCAAGGATC
TGGAAATCGGCGCCCAGAAGGCCAAGGCGATTTCCTCCAATGAGGAATACCGCGAGGCTCGCCCCGGCCAGATCGAAGTG
CCCCGCGCGGGTATGATCGAAGCGGTGCGCGCAATTGTCGAGGCCCAGCGATGA

Upstream 100 bases:

>100_bases
CCTTTGTGCCGCCTTTCCAACCCGGCCGCCTGCGTTTATGCGGGGCCACAGATAGCCAAGCCGATAGCCAAATTGGCCGC
AAACAGGATCTGAGACGACC

Downstream 100 bases:

>100_bases
CCCATGACGCTTTGGTTGCCGTTGCCCGCGCTGTTTTCGAGGCGGCGGGCGGCACGCCGATTGATCCGGATTATGTTCTG
CCAGCGGACATTCCGCTGGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSDKDNQPLSGPLSGKDLARKPRGFPDKRESLIHAQARLVETVTGVYRQWGFEALDTGAFEYADALGKFLPDADRPNAGV
FSMQDDDAQWMALRYDLTAPLARFVAEQGQGLGRPFRRYAAGPVWRNEKPGPGRFREFWQCDADTVGAPGFHADAEMIAM
GAEALRAIGMKPGEFVIRVNTRRLLNGVLDGVGATGADIRLAILRALDKLDRLGASGVADLLGAGRKDESGDFTKGAGLG
PEEIKRVLAFAEAGADTRAMTLANLAKATGSTEEGKAGLGELEAIALALEALGAPEDDVKIDPSVVRGLEYYTGPVFEAE
LLREVTGEDGKTYRIGSIGGGGRYDDLVARFTGETVPATGFSIGISRLAAALAIMGEGGKLDGPVVVLNLDKDNPGVALG
LAAELRRGGIRAEAYMGASGMRPQMKYADRRNSPAVVIVGGDELEKGTVTIKDLEIGAQKAKAISSNEEYREARPGQIEV
PRAGMIEAVRAIVEAQR

Sequences:

>Translated_497_residues
MSDKDNQPLSGPLSGKDLARKPRGFPDKRESLIHAQARLVETVTGVYRQWGFEALDTGAFEYADALGKFLPDADRPNAGV
FSMQDDDAQWMALRYDLTAPLARFVAEQGQGLGRPFRRYAAGPVWRNEKPGPGRFREFWQCDADTVGAPGFHADAEMIAM
GAEALRAIGMKPGEFVIRVNTRRLLNGVLDGVGATGADIRLAILRALDKLDRLGASGVADLLGAGRKDESGDFTKGAGLG
PEEIKRVLAFAEAGADTRAMTLANLAKATGSTEEGKAGLGELEAIALALEALGAPEDDVKIDPSVVRGLEYYTGPVFEAE
LLREVTGEDGKTYRIGSIGGGGRYDDLVARFTGETVPATGFSIGISRLAAALAIMGEGGKLDGPVVVLNLDKDNPGVALG
LAAELRRGGIRAEAYMGASGMRPQMKYADRRNSPAVVIVGGDELEKGTVTIKDLEIGAQKAKAISSNEEYREARPGQIEV
PRAGMIEAVRAIVEAQR
>Mature_496_residues
SDKDNQPLSGPLSGKDLARKPRGFPDKRESLIHAQARLVETVTGVYRQWGFEALDTGAFEYADALGKFLPDADRPNAGVF
SMQDDDAQWMALRYDLTAPLARFVAEQGQGLGRPFRRYAAGPVWRNEKPGPGRFREFWQCDADTVGAPGFHADAEMIAMG
AEALRAIGMKPGEFVIRVNTRRLLNGVLDGVGATGADIRLAILRALDKLDRLGASGVADLLGAGRKDESGDFTKGAGLGP
EEIKRVLAFAEAGADTRAMTLANLAKATGSTEEGKAGLGELEAIALALEALGAPEDDVKIDPSVVRGLEYYTGPVFEAEL
LREVTGEDGKTYRIGSIGGGGRYDDLVARFTGETVPATGFSIGISRLAAALAIMGEGGKLDGPVVVLNLDKDNPGVALGL
AAELRRGGIRAEAYMGASGMRPQMKYADRRNSPAVVIVGGDELEKGTVTIKDLEIGAQKAKAISSNEEYREARPGQIEVP
RAGMIEAVRAIVEAQR

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=449, Percent_Identity=30.2895322939866, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI6996014, Length=487, Percent_Identity=27.7207392197125, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788861, Length=397, Percent_Identity=28.9672544080605, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71993686, Length=443, Percent_Identity=30.2483069977427, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71993693, Length=443, Percent_Identity=30.2483069977427, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325290, Length=475, Percent_Identity=28.6315789473684, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643061, Length=479, Percent_Identity=28.8100208768267, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643059, Length=498, Percent_Identity=27.5100401606426, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 52829; Mature: 52697

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKDNQPLSGPLSGKDLARKPRGFPDKRESLIHAQARLVETVTGVYRQWGFEALDTGAF
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
EYADALGKFLPDADRPNAGVFSMQDDDAQWMALRYDLTAPLARFVAEQGQGLGRPFRRYA
HHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHH
AGPVWRNEKPGPGRFREFWQCDADTVGAPGFHADAEMIAMGAEALRAIGMKPGEFVIRVN
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
TRRLLNGVLDGVGATGADIRLAILRALDKLDRLGASGVADLLGAGRKDESGDFTKGAGLG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCC
PEEIKRVLAFAEAGADTRAMTLANLAKATGSTEEGKAGLGELEAIALALEALGAPEDDVK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
IDPSVVRGLEYYTGPVFEAELLREVTGEDGKTYRIGSIGGGGRYDDLVARFTGETVPATG
ECHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCH
FSIGISRLAAALAIMGEGGKLDGPVVVLNLDKDNPGVALGLAAELRRGGIRAEAYMGASG
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCC
MRPQMKYADRRNSPAVVIVGGDELEKGTVTIKDLEIGAQKAKAISSNEEYREARPGQIEV
CCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCHHHHHHHCCCHHHHHCCCCEEEC
PRAGMIEAVRAIVEAQR
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDKDNQPLSGPLSGKDLARKPRGFPDKRESLIHAQARLVETVTGVYRQWGFEALDTGAF
CCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
EYADALGKFLPDADRPNAGVFSMQDDDAQWMALRYDLTAPLARFVAEQGQGLGRPFRRYA
HHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHH
AGPVWRNEKPGPGRFREFWQCDADTVGAPGFHADAEMIAMGAEALRAIGMKPGEFVIRVN
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
TRRLLNGVLDGVGATGADIRLAILRALDKLDRLGASGVADLLGAGRKDESGDFTKGAGLG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCC
PEEIKRVLAFAEAGADTRAMTLANLAKATGSTEEGKAGLGELEAIALALEALGAPEDDVK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
IDPSVVRGLEYYTGPVFEAELLREVTGEDGKTYRIGSIGGGGRYDDLVARFTGETVPATG
ECHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCH
FSIGISRLAAALAIMGEGGKLDGPVVVLNLDKDNPGVALGLAAELRRGGIRAEAYMGASG
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCC
MRPQMKYADRRNSPAVVIVGGDELEKGTVTIKDLEIGAQKAKAISSNEEYREARPGQIEV
CCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCHHHHHHHCCCHHHHHCCCCEEEC
PRAGMIEAVRAIVEAQR
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]