| Definition | Nitrosomonas eutropha C91, complete genome. |
|---|---|
| Accession | NC_008344 |
| Length | 2,661,057 |
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The map label for this gene is metE [H]
Identifier: 114331542
GI number: 114331542
Start: 1625801
End: 1628110
Strand: Direct
Name: metE [H]
Synonym: Neut_1555
Alternate gene names: 114331542
Gene position: 1625801-1628110 (Clockwise)
Preceding gene: 114331540
Following gene: 114331544
Centisome position: 61.1
GC content: 48.44
Gene sequence:
>2310_bases ATGATGGTTTTCCGATCATGCAGAGAAAATATAATGAAAATACTGACACATAATCTCGGCTTCCCGAGAATCGGTGCACA ACGTGAATTAAAAAAAGCACTCGAAGCTCACTGGAAAGGACAAAGTGAGGCTGCGCAATTATTGGCTACTGCGCGCGAAA TTCGCGCCAGCAACTGGCTATCACAGCAAAAATCTGGCATTGATTTGATCCCGGTGGGAGATTTCTCACTGTATGATCAC ATACTCGACATGACCGCATTACTGGGAGCAATTCCCCATCGCTTCGAGCACACCGGCGATCAGGTTCCGCTTGATCTGTA CTTTGCCATGGCACGTGGCACTGTTAATCAACCAGCTATGGAAATGACCAAGTGGTTCAATACCAATTATCACTACATCG TTCCTGAATTCGATCACAACACCCGATTTCATCTGGCATCCGACTGGCTGTTCCAGGAAATTAAAGAAGCCAAAGTGCTG GGGATAAATACAAAAGTTGTTTTGATCGGCCCGCTGACCTACCTGTATCTTGGTAAAGAAATTACGCCAGATTTTCAGCG ATTGGATCTGCTTCCCAGCCTGCTGCCGGTTTATCGCGAAATTCTGCAGCAAATTGTTGCACTGGGTGTGGAATGGGTGC AGATTGACGAACCTATCCTCGCTCTTGATCTCGAGCAGCCCTGGCTAGATAGTTTTACAGAGGCCTATCGTGCACTAAAT GATACGGGCAGTCGCTTGCTGCTCACCTCTTACTTTGGGGCGGTTGATCACCACCTCGCATTGCTTGAGAACTTGCCGGT AGATGGCCTGCATATTGACCTCAGCAGTGCTCCGGAACAACTCGAATCATTCCTCACCAGAAATTTCTCCAGCAAAATCC TGTCACTGGGTTGTATCGACGGACGCAATATCTGGCGCACTGATCTATCACAAAAACTGGAAAACTTAGCACAAACTGTA TCTCGATTTAAGGGCAATCTGTGGATTGCCCCGAGTTGTTCCTTGCTACATTGCCCGGTAGATTTATCATTAGAAACTAA ACTGGATCCAGAAATTAGAAATTGGCTAGCCTTTTCTACTCAGAAACTGGAAGAAATCGCCACACTCGGGCTCGGACTGT ATCAGGGTAAGGAATCAATCCAAGAGATACTGACTGCTTCCAATCAAGCCAACCAATCAAGAAAGACATCTTGTCGCATC CATAACCCGGTGGTTCATCACAGAATTAACCATCTGACTGAACGAGACAGCCAGCGCACGCATCCTTTTGCAATTCGTAA ACAACAGCAACAGCAGCGCTTCAATCTGCCTCTGTTGCCCACCACCACCATTGGTTCTTTCCCGCAAACTTCCGCCATTC GCCAGGTACGCGCCGCATTCAAAAAAAATGAACTAAGTCATCTTGAATACCTGTCTGCCATGCGTCAAGAAATTTGCGAG ACTATCCGCAAACAGGAAGAAATCGGACTGGATGTGCTGGTTCATGGCGAACCCGAACGTAATGATATGGTGGAATATTT CGGCGAACAGCTGTGGGGATATGCTTTCACTGAAAACGGCTGGGTGCAAAGCTACGGCTCCCGTTGCGTCAAACCACCTA TTTTATATGGTGATGTCTATCGCCCTGAGGCCATGACAGTCGATTGGATCAGCTATGCCCAAAGCCAGACCAGCAGACCA GTTAAAGGCATGTTGACCGGGCCAGTCACTATGCTGTTCTGGTCATTCGTGCGAGATGATCAGCCACGGGCAGATACCGC TTTACAACTGGCACTGGCAATTCGTGATGAAGTCACTGATCTGGAAAAAGCCGGTATCGGCATGATTCAGATTGATGAGC CGGCCTTTCGCGAAGGACTCCCGCTCAGAAGACAGGACTGGCAGCATTACCTTGACTGGGCAGTCAAGGCGTTTCGCGTT GCCAGCTCGGGGGTAAAGGATGAAACTCAGATCCACACCCACATGTGTTATTCCGAATTTCATGACATCCTGCCCGCCAT CGCCGAACTAGATGCAGATGTCATCACAATTGAGACTTCACGTTCGCGTATGGAATTGCTGGATGCATTCGTAAAATTTT CCTACCCGAATGAAATCGGCCCTGGAGTATATGACATTCACTCTCCCCGTATCCCTGATACATCGGAAATGCTGGAGCTG CTGAAAAAGGCATCACACCATATTGATCCAAAGCTACTCTGGGTCAACCCGGATTGTGGCCTGAAAACGCGCAACTGGCC GGAAACCCGGACAGCTCTGCAAAAAATGGTGGATTGTGCAAAATCCCTGCGCAGCGCCCTGCAAACCTGA
Upstream 100 bases:
>100_bases CATTCAATGTGCGAAGATGTCGTAATTCAAGCATATATGAAAATTATTCATAATTATCTGTAAAACATTTCATTTGATTC ATTATAGAGCATTCCACACA
Downstream 100 bases:
>100_bases TCTATTGTCTATCCCTCCGCGCAGTTGCCGGGCATAATCCAGGCTGTCACCCCCCTCTTCTGACAGCTTTGTCCGTGTAA CTGCCACCTCACCCACGGTA
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 769; Mature: 769
Protein sequence:
>769_residues MMVFRSCRENIMKILTHNLGFPRIGAQRELKKALEAHWKGQSEAAQLLATAREIRASNWLSQQKSGIDLIPVGDFSLYDH ILDMTALLGAIPHRFEHTGDQVPLDLYFAMARGTVNQPAMEMTKWFNTNYHYIVPEFDHNTRFHLASDWLFQEIKEAKVL GINTKVVLIGPLTYLYLGKEITPDFQRLDLLPSLLPVYREILQQIVALGVEWVQIDEPILALDLEQPWLDSFTEAYRALN DTGSRLLLTSYFGAVDHHLALLENLPVDGLHIDLSSAPEQLESFLTRNFSSKILSLGCIDGRNIWRTDLSQKLENLAQTV SRFKGNLWIAPSCSLLHCPVDLSLETKLDPEIRNWLAFSTQKLEEIATLGLGLYQGKESIQEILTASNQANQSRKTSCRI HNPVVHHRINHLTERDSQRTHPFAIRKQQQQQRFNLPLLPTTTIGSFPQTSAIRQVRAAFKKNELSHLEYLSAMRQEICE TIRKQEEIGLDVLVHGEPERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPILYGDVYRPEAMTVDWISYAQSQTSRP VKGMLTGPVTMLFWSFVRDDQPRADTALQLALAIRDEVTDLEKAGIGMIQIDEPAFREGLPLRRQDWQHYLDWAVKAFRV ASSGVKDETQIHTHMCYSEFHDILPAIAELDADVITIETSRSRMELLDAFVKFSYPNEIGPGVYDIHSPRIPDTSEMLEL LKKASHHIDPKLLWVNPDCGLKTRNWPETRTALQKMVDCAKSLRSALQT
Sequences:
>Translated_769_residues MMVFRSCRENIMKILTHNLGFPRIGAQRELKKALEAHWKGQSEAAQLLATAREIRASNWLSQQKSGIDLIPVGDFSLYDH ILDMTALLGAIPHRFEHTGDQVPLDLYFAMARGTVNQPAMEMTKWFNTNYHYIVPEFDHNTRFHLASDWLFQEIKEAKVL GINTKVVLIGPLTYLYLGKEITPDFQRLDLLPSLLPVYREILQQIVALGVEWVQIDEPILALDLEQPWLDSFTEAYRALN DTGSRLLLTSYFGAVDHHLALLENLPVDGLHIDLSSAPEQLESFLTRNFSSKILSLGCIDGRNIWRTDLSQKLENLAQTV SRFKGNLWIAPSCSLLHCPVDLSLETKLDPEIRNWLAFSTQKLEEIATLGLGLYQGKESIQEILTASNQANQSRKTSCRI HNPVVHHRINHLTERDSQRTHPFAIRKQQQQQRFNLPLLPTTTIGSFPQTSAIRQVRAAFKKNELSHLEYLSAMRQEICE TIRKQEEIGLDVLVHGEPERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPILYGDVYRPEAMTVDWISYAQSQTSRP VKGMLTGPVTMLFWSFVRDDQPRADTALQLALAIRDEVTDLEKAGIGMIQIDEPAFREGLPLRRQDWQHYLDWAVKAFRV ASSGVKDETQIHTHMCYSEFHDILPAIAELDADVITIETSRSRMELLDAFVKFSYPNEIGPGVYDIHSPRIPDTSEMLEL LKKASHHIDPKLLWVNPDCGLKTRNWPETRTALQKMVDCAKSLRSALQT >Mature_769_residues MMVFRSCRENIMKILTHNLGFPRIGAQRELKKALEAHWKGQSEAAQLLATAREIRASNWLSQQKSGIDLIPVGDFSLYDH ILDMTALLGAIPHRFEHTGDQVPLDLYFAMARGTVNQPAMEMTKWFNTNYHYIVPEFDHNTRFHLASDWLFQEIKEAKVL GINTKVVLIGPLTYLYLGKEITPDFQRLDLLPSLLPVYREILQQIVALGVEWVQIDEPILALDLEQPWLDSFTEAYRALN DTGSRLLLTSYFGAVDHHLALLENLPVDGLHIDLSSAPEQLESFLTRNFSSKILSLGCIDGRNIWRTDLSQKLENLAQTV SRFKGNLWIAPSCSLLHCPVDLSLETKLDPEIRNWLAFSTQKLEEIATLGLGLYQGKESIQEILTASNQANQSRKTSCRI HNPVVHHRINHLTERDSQRTHPFAIRKQQQQQRFNLPLLPTTTIGSFPQTSAIRQVRAAFKKNELSHLEYLSAMRQEICE TIRKQEEIGLDVLVHGEPERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPILYGDVYRPEAMTVDWISYAQSQTSRP VKGMLTGPVTMLFWSFVRDDQPRADTALQLALAIRDEVTDLEKAGIGMIQIDEPAFREGLPLRRQDWQHYLDWAVKAFRV ASSGVKDETQIHTHMCYSEFHDILPAIAELDADVITIETSRSRMELLDAFVKFSYPNEIGPGVYDIHSPRIPDTSEMLEL LKKASHHIDPKLLWVNPDCGLKTRNWPETRTALQKMVDCAKSLRSALQT
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=54.8216644649934, Blast_Score=838, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=763, Percent_Identity=47.9685452162516, Blast_Score=687, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 87864; Mature: 87864
Theoretical pI: Translated: 6.26; Mature: 6.26
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMVFRSCRENIMKILTHNLGFPRIGAQRELKKALEAHWKGQSEAAQLLATAREIRASNWL CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH SQQKSGIDLIPVGDFSLYDHILDMTALLGAIPHRFEHTGDQVPLDLYFAMARGTVNQPAM HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHH EMTKWFNTNYHYIVPEFDHNTRFHLASDWLFQEIKEAKVLGINTKVVLIGPLTYLYLGKE HHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEECCHHHHHCCCC ITPDFQRLDLLPSLLPVYREILQQIVALGVEWVQIDEPILALDLEQPWLDSFTEAYRALN CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHH DTGSRLLLTSYFGAVDHHLALLENLPVDGLHIDLSSAPEQLESFLTRNFSSKILSLGCID CCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCHHHHHHEEECCC GRNIWRTDLSQKLENLAQTVSRFKGNLWIAPSCSLLHCPVDLSLETKLDPEIRNWLAFST CCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHH QKLEEIATLGLGLYQGKESIQEILTASNQANQSRKTSCRIHNPVVHHRINHLTERDSQRT HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHCCCEECCHHHHHHHHHHHHCCCCCC HPFAIRKQQQQQRFNLPLLPTTTIGSFPQTSAIRQVRAAFKKNELSHLEYLSAMRQEICE CCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TIRKQEEIGLDVLVHGEPERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPILYGDVY HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCCCCEEECCCC RPEAMTVDWISYAQSQTSRPVKGMLTGPVTMLFWSFVRDDQPRADTALQLALAIRDEVTD CCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH LEKAGIGMIQIDEPAFREGLPLRRQDWQHYLDWAVKAFRVASSGVKDETQIHTHMCYSEF HHHCCCCEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH HDILPAIAELDADVITIETSRSRMELLDAFVKFSYPNEIGPGVYDIHSPRIPDTSEMLEL HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHH LKKASHHIDPKLLWVNPDCGLKTRNWPETRTALQKMVDCAKSLRSALQT HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MMVFRSCRENIMKILTHNLGFPRIGAQRELKKALEAHWKGQSEAAQLLATAREIRASNWL CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH SQQKSGIDLIPVGDFSLYDHILDMTALLGAIPHRFEHTGDQVPLDLYFAMARGTVNQPAM HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHH EMTKWFNTNYHYIVPEFDHNTRFHLASDWLFQEIKEAKVLGINTKVVLIGPLTYLYLGKE HHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEECCHHHHHCCCC ITPDFQRLDLLPSLLPVYREILQQIVALGVEWVQIDEPILALDLEQPWLDSFTEAYRALN CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHH DTGSRLLLTSYFGAVDHHLALLENLPVDGLHIDLSSAPEQLESFLTRNFSSKILSLGCID CCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCHHHHHHEEECCC GRNIWRTDLSQKLENLAQTVSRFKGNLWIAPSCSLLHCPVDLSLETKLDPEIRNWLAFST CCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHH QKLEEIATLGLGLYQGKESIQEILTASNQANQSRKTSCRIHNPVVHHRINHLTERDSQRT HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHCCCEECCHHHHHHHHHHHHCCCCCC HPFAIRKQQQQQRFNLPLLPTTTIGSFPQTSAIRQVRAAFKKNELSHLEYLSAMRQEICE CCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TIRKQEEIGLDVLVHGEPERNDMVEYFGEQLWGYAFTENGWVQSYGSRCVKPPILYGDVY HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCCCCEEECCCC RPEAMTVDWISYAQSQTSRPVKGMLTGPVTMLFWSFVRDDQPRADTALQLALAIRDEVTD CCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH LEKAGIGMIQIDEPAFREGLPLRRQDWQHYLDWAVKAFRVASSGVKDETQIHTHMCYSEF HHHCCCCEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH HDILPAIAELDADVITIETSRSRMELLDAFVKFSYPNEIGPGVYDIHSPRIPDTSEMLEL HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHH LKKASHHIDPKLLWVNPDCGLKTRNWPETRTALQKMVDCAKSLRSALQT HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12700255 [H]