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The map label for this gene is tyrS [H]

Identifier: 114331532

GI number: 114331532

Start: 1613594

End: 1614814

Strand: Direct

Name: tyrS [H]

Synonym: Neut_1545

Alternate gene names: 114331532

Gene position: 1613594-1614814 (Clockwise)

Preceding gene: 114331529

Following gene: 114331536

Centisome position: 60.64

GC content: 47.83

Gene sequence:

>1221_bases
GTGTCTGAGTCAATTATTCATCAACTTCAAGTAATCAAACGGGGCTGTCAGGAGCTGCTGATTGAGGAAGAACTCACACA
AAAACTCACCAAAAATCAACCCCTAAGGATCAAAGCCGGCTTTGATCCTACCGCACCGGATCTGCACTTGGGGCATACCG
TTCTGCTGAACAAACTGCGCCAGCTTCAGGAGCTTGGCCACCATATCCTGTTCCTGATTGGTGACTTTACTGGAATGATC
GGCGACCCCAGCGGCAAAAACGCCACGCGCCCACCGCTGACATGTGAGCAGATTGCACAAAATGCAGAAACCTACACATC
TCAGGTATTCAAGATACTTAAACCTGAGCAAACAGAGGTGGTTTTCAATTCAACCTGGATGGATAAATTCAGTGCAGCTG
ACCTGGTCAGACTGGCCTCCACTTATACCGTCGCACGCATGCTTGAACGGGATGATTTCAGCAAGCGCTATCACGAGAAT
CGGCCGATCGCCATCCATGAATTTCTTTATCCGCTTGTGCAGGGTTACGATTCAGTAGCGCTGAAAACCGATCTCGAACT
AGGCGGCACTGATCAAAAATTCAATTTGCTGGTAGGGCGTGAATTACAGAAGCACTACGGCCAATCTCCGCAGTGCATAC
TGACCATGCCACTTTTGGAAGGGCTGGATGGTATCCAGAAAATGTCGAAATCGCTCAACAACTATGTCGGTATTAATGAA
TCGCCTACTGAAATGTTCGGCAAGCTGATGTCAATCAGCGACACCCTGATGTGGCGTTACATCGAATTACTGTCTTTTGA
ATCACTGGACACCATACACCAGTGGCAGCTTGAAGTGGAAGCAGGCCGAAATCCGCGTGACATCAAGGTGCAGTTTGCAC
AGGAAATTGTGGCGCGCTTTCATAGCCGAACAGCTGCTATTCAAGCTACAGAAGATTTTGATGCTCGTTTCAGAAAAGGT
GCCATTCCTGATGATATTCCAGAAAAGAAACTGTATGTTCAAGGCACAGAGCTGGCTGCACCTCAACTGCTCAAGCTGAC
TGGATTAACCGCCAGTACCAGTGAAGCCCTGCGTATGATTGAACAGGGTGGCGTAAAGCTCAATGGCAACAAGGTCAGTG
ACAAAACCCAAACTGTTCCCGTCAATACGACAGTTATTGCCCAGGTCGGGAAGAGGAAATTCGCAAAAATAATACTTTTG
CCCGATCAAGATGGCATATAA

Upstream 100 bases:

>100_bases
TAAATCAAATGCCATCACTCCTATTCGTCTGTTGGAAATTTTTCAAGTCTAACACGGAAAAGCATAAATATAATGACGAC
TGATTTTTTACTGGCTGGCC

Downstream 100 bases:

>100_bases
AATCTGCTTCTCTGTAAAATCAATTCCAGCTATATAGCTTATATGACAACATTGTTTATCCACCCCGAAAAATATTAATA
TGTAGACTTGGTTGTAACGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSESIIHQLQVIKRGCQELLIEEELTQKLTKNQPLRIKAGFDPTAPDLHLGHTVLLNKLRQLQELGHHILFLIGDFTGMI
GDPSGKNATRPPLTCEQIAQNAETYTSQVFKILKPEQTEVVFNSTWMDKFSAADLVRLASTYTVARMLERDDFSKRYHEN
RPIAIHEFLYPLVQGYDSVALKTDLELGGTDQKFNLLVGRELQKHYGQSPQCILTMPLLEGLDGIQKMSKSLNNYVGINE
SPTEMFGKLMSISDTLMWRYIELLSFESLDTIHQWQLEVEAGRNPRDIKVQFAQEIVARFHSRTAAIQATEDFDARFRKG
AIPDDIPEKKLYVQGTELAAPQLLKLTGLTASTSEALRMIEQGGVKLNGNKVSDKTQTVPVNTTVIAQVGKRKFAKIILL
PDQDGI

Sequences:

>Translated_406_residues
MSESIIHQLQVIKRGCQELLIEEELTQKLTKNQPLRIKAGFDPTAPDLHLGHTVLLNKLRQLQELGHHILFLIGDFTGMI
GDPSGKNATRPPLTCEQIAQNAETYTSQVFKILKPEQTEVVFNSTWMDKFSAADLVRLASTYTVARMLERDDFSKRYHEN
RPIAIHEFLYPLVQGYDSVALKTDLELGGTDQKFNLLVGRELQKHYGQSPQCILTMPLLEGLDGIQKMSKSLNNYVGINE
SPTEMFGKLMSISDTLMWRYIELLSFESLDTIHQWQLEVEAGRNPRDIKVQFAQEIVARFHSRTAAIQATEDFDARFRKG
AIPDDIPEKKLYVQGTELAAPQLLKLTGLTASTSEALRMIEQGGVKLNGNKVSDKTQTVPVNTTVIAQVGKRKFAKIILL
PDQDGI
>Mature_405_residues
SESIIHQLQVIKRGCQELLIEEELTQKLTKNQPLRIKAGFDPTAPDLHLGHTVLLNKLRQLQELGHHILFLIGDFTGMIG
DPSGKNATRPPLTCEQIAQNAETYTSQVFKILKPEQTEVVFNSTWMDKFSAADLVRLASTYTVARMLERDDFSKRYHENR
PIAIHEFLYPLVQGYDSVALKTDLELGGTDQKFNLLVGRELQKHYGQSPQCILTMPLLEGLDGIQKMSKSLNNYVGINES
PTEMFGKLMSISDTLMWRYIELLSFESLDTIHQWQLEVEAGRNPRDIKVQFAQEIVARFHSRTAAIQATEDFDARFRKGA
IPDDIPEKKLYVQGTELAAPQLLKLTGLTASTSEALRMIEQGGVKLNGNKVSDKTQTVPVNTTVIAQVGKRKFAKIILLP
DQDGI

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=417, Percent_Identity=27.0983213429257, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1787925, Length=400, Percent_Identity=29.5, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=208, Percent_Identity=33.1730769230769, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=274, Percent_Identity=28.1021897810219, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130383, Length=414, Percent_Identity=24.8792270531401, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45718; Mature: 45587

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESIIHQLQVIKRGCQELLIEEELTQKLTKNQPLRIKAGFDPTAPDLHLGHTVLLNKLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
QLQELGHHILFLIGDFTGMIGDPSGKNATRPPLTCEQIAQNAETYTSQVFKILKPEQTEV
HHHHHHHHHEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VFNSTWMDKFSAADLVRLASTYTVARMLERDDFSKRYHENRPIAIHEFLYPLVQGYDSVA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEE
LKTDLELGGTDQKFNLLVGRELQKHYGQSPQCILTMPLLEGLDGIQKMSKSLNNYVGINE
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHCHHHHHHHHHHHHHCCCCCC
SPTEMFGKLMSISDTLMWRYIELLSFESLDTIHQWQLEVEAGRNPRDIKVQFAQEIVARF
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
HSRTAAIQATEDFDARFRKGAIPDDIPEKKLYVQGTELAAPQLLKLTGLTASTSEALRMI
HHHHHEEEECHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHH
EQGGVKLNGNKVSDKTQTVPVNTTVIAQVGKRKFAKIILLPDQDGI
HHCCEEECCCCCCCCCEEECCCHHHHHHHHHCCEEEEEEECCCCCC
>Mature Secondary Structure 
SESIIHQLQVIKRGCQELLIEEELTQKLTKNQPLRIKAGFDPTAPDLHLGHTVLLNKLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
QLQELGHHILFLIGDFTGMIGDPSGKNATRPPLTCEQIAQNAETYTSQVFKILKPEQTEV
HHHHHHHHHEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VFNSTWMDKFSAADLVRLASTYTVARMLERDDFSKRYHENRPIAIHEFLYPLVQGYDSVA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEE
LKTDLELGGTDQKFNLLVGRELQKHYGQSPQCILTMPLLEGLDGIQKMSKSLNNYVGINE
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHCHHHHHHHHHHHHHCCCCCC
SPTEMFGKLMSISDTLMWRYIELLSFESLDTIHQWQLEVEAGRNPRDIKVQFAQEIVARF
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
HSRTAAIQATEDFDARFRKGAIPDDIPEKKLYVQGTELAAPQLLKLTGLTASTSEALRMI
HHHHHEEEECHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHH
EQGGVKLNGNKVSDKTQTVPVNTTVIAQVGKRKFAKIILLPDQDGI
HHCCEEECCCCCCCCCEEECCCHHHHHHHHHCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]