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The map label for this gene is ispF

Identifier: 114331294

GI number: 114331294

Start: 1365361

End: 1365864

Strand: Reverse

Name: ispF

Synonym: Neut_1300

Alternate gene names: 114331294

Gene position: 1365864-1365361 (Counterclockwise)

Preceding gene: 114331295

Following gene: 114331293

Centisome position: 51.33

GC content: 50.6

Gene sequence:

>504_bases
ATGAAAAAAATCAGAATCGGACAAGGGTTTGATATTCACCAGTTAGCTGCCGGGCGTGAGCTTGTCATTGGTGGCGTGAC
TATTCCGTATGAGAAGGGTCTGCTGGGCCACTCGGATGCGGATGTGCTGCTGCATGCCTTGTGTGATGCGCTGCTGGGTG
CTGCCGCCTTGGGGGATATTGGCAAGCATTTTTCCGATACCGATGCACGTTATAAAGATATTGATAGTCGCAAGCTGGTG
TGTAAAGTTCACCGGTTGCTGGCTGAAACGGGTTATCGTGTTGTGAATATCGATGCGACGATCATTGCCCAAGCACCTAA
AATGGCCCCCCATATTTCCGGAATGATAGCCAACATTGCACAGGATCTCGCCATGCCTGCTGGAAATATTAATATCAAGG
CAAAAACTGCCGAGAAACTCGGTGCTGTCGGGCGTGGTGAAGGTATCGTAGCGGAAGTGGTCTGCCTGATCGCTAACAGT
GACGAAGGCGAAGCACAGCCATAA

Upstream 100 bases:

>100_bases
TATCCGTACAGGAGTGTGCAGGTGGGGCAATCAGATGATTTCTGCTGTTTCCTCAGAACTTGAGTAGCTTGCTCACTTCA
GAACATATTACCCAGTTATC

Downstream 100 bases:

>100_bases
TGTCCGGCGCTGCTCATCCTGTCAGGCTGTTCCTTGCATTGTGGCCACAGGATATCGCAAGAGATCAATTGGCCAAACTG
GCGCGGCAGCTGGCGGATGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MKKIRIGQGFDIHQLAAGRELVIGGVTIPYEKGLLGHSDADVLLHALCDALLGAAALGDIGKHFSDTDARYKDIDSRKLV
CKVHRLLAETGYRVVNIDATIIAQAPKMAPHISGMIANIAQDLAMPAGNINIKAKTAEKLGAVGRGEGIVAEVVCLIANS
DEGEAQP

Sequences:

>Translated_167_residues
MKKIRIGQGFDIHQLAAGRELVIGGVTIPYEKGLLGHSDADVLLHALCDALLGAAALGDIGKHFSDTDARYKDIDSRKLV
CKVHRLLAETGYRVVNIDATIIAQAPKMAPHISGMIANIAQDLAMPAGNINIKAKTAEKLGAVGRGEGIVAEVVCLIANS
DEGEAQP
>Mature_167_residues
MKKIRIGQGFDIHQLAAGRELVIGGVTIPYEKGLLGHSDADVLLHALCDALLGAAALGDIGKHFSDTDARYKDIDSRKLV
CKVHRLLAETGYRVVNIDATIIAQAPKMAPHISGMIANIAQDLAMPAGNINIKAKTAEKLGAVGRGEGIVAEVVCLIANS
DEGEAQP

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=58.4415584415584, Blast_Score=167, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_NITEC (Q0AGI1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747516.1
- ProteinModelPortal:   Q0AGI1
- SMR:   Q0AGI1
- STRING:   Q0AGI1
- GeneID:   4272297
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1300
- NMPDR:   fig|335283.3.peg.1341
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q0AGI1
- ProtClustDB:   PRK00084
- BioCyc:   NEUT335283:NEUT_1300-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17572; Mature: 17572

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIRIGQGFDIHQLAAGRELVIGGVTIPYEKGLLGHSDADVLLHALCDALLGAAALGDI
CCCCCCCCCCCHHHHHCCCEEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GKHFSDTDARYKDIDSRKLVCKVHRLLAETGYRVVNIDATIIAQAPKMAPHISGMIANIA
HHCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCHHHHHHHHHHH
QDLAMPAGNINIKAKTAEKLGAVGRGEGIVAEVVCLIANSDEGEAQP
HHHCCCCCCEEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKIRIGQGFDIHQLAAGRELVIGGVTIPYEKGLLGHSDADVLLHALCDALLGAAALGDI
CCCCCCCCCCCHHHHHCCCEEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GKHFSDTDARYKDIDSRKLVCKVHRLLAETGYRVVNIDATIIAQAPKMAPHISGMIANIA
HHCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCHHHHHHHHHHH
QDLAMPAGNINIKAKTAEKLGAVGRGEGIVAEVVCLIANSDEGEAQP
HHHCCCCCCEEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA