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The map label for this gene is ligT [H]

Identifier: 114331293

GI number: 114331293

Start: 1364828

End: 1365361

Strand: Reverse

Name: ligT [H]

Synonym: Neut_1299

Alternate gene names: 114331293

Gene position: 1365361-1364828 (Counterclockwise)

Preceding gene: 114331294

Following gene: 114331292

Centisome position: 51.31

GC content: 49.63

Gene sequence:

>534_bases
ATGTCCGGCGCTGCTCATCCTGTCAGGCTGTTCCTTGCATTGTGGCCACAGGATATCGCAAGAGATCAATTGGCCAAACT
GGCGCGGCAGCTGGCGGATGATTGTTCAGGTCGTTGCATTCAACCGAAAAATCTACATCTGACATTGGCATTCATGGGCG
AAATCGATCAAGGTAATATTCCACGATTGTGTCAGGCAGCCCCGGCTATCCGGCAATCTCCATTCAATTTGACTCTGGAT
AAGGTTTGCTTCTGGAAAAAAGCCGGAGTCGTGGTGGCCGGAACAAGCCAATGTCCAGCAGAATTAACTACTCTTGTGCA
GGATCTGCAGCACTTGCTTTCTGCCTTGAGAATCCGGTATGACGACACATATCCATTTACACCACACGTCACGCTGGTAA
GAAATGTAAGGCACTGCAGCTTGCCTGATTCAATCCAAGCGATTGATTGGCCAGTGACTCAATGGTCGTTGGCACAATCC
CGGCAGACACCATACGAATCAGTCTATCAATCTCTTGCAGACTGGGCACTGTGA

Upstream 100 bases:

>100_bases
AAACTGCCGAGAAACTCGGTGCTGTCGGGCGTGGTGAAGGTATCGTAGCGGAAGTGGTCTGCCTGATCGCTAACAGTGAC
GAAGGCGAAGCACAGCCATA

Downstream 100 bases:

>100_bases
ACCACAGGATTCCGATGAACATCATTGCGTTTGATACTTCTACCGAATACTGTTCCCTGGCTCTGTGGCTGAATGGAAAC
ATTATTAGCAGGGAAGAGTT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MSGAAHPVRLFLALWPQDIARDQLAKLARQLADDCSGRCIQPKNLHLTLAFMGEIDQGNIPRLCQAAPAIRQSPFNLTLD
KVCFWKKAGVVVAGTSQCPAELTTLVQDLQHLLSALRIRYDDTYPFTPHVTLVRNVRHCSLPDSIQAIDWPVTQWSLAQS
RQTPYESVYQSLADWAL

Sequences:

>Translated_177_residues
MSGAAHPVRLFLALWPQDIARDQLAKLARQLADDCSGRCIQPKNLHLTLAFMGEIDQGNIPRLCQAAPAIRQSPFNLTLD
KVCFWKKAGVVVAGTSQCPAELTTLVQDLQHLLSALRIRYDDTYPFTPHVTLVRNVRHCSLPDSIQAIDWPVTQWSLAQS
RQTPYESVYQSLADWAL
>Mature_176_residues
SGAAHPVRLFLALWPQDIARDQLAKLARQLADDCSGRCIQPKNLHLTLAFMGEIDQGNIPRLCQAAPAIRQSPFNLTLDK
VCFWKKAGVVVAGTSQCPAELTTLVQDLQHLLSALRIRYDDTYPFTPHVTLVRNVRHCSLPDSIQAIDWPVTQWSLAQSR
QTPYESVYQSLADWAL

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=175, Percent_Identity=33.1428571428571, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19836; Mature: 19705

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAAHPVRLFLALWPQDIARDQLAKLARQLADDCSGRCIQPKNLHLTLAFMGEIDQGNI
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCC
PRLCQAAPAIRQSPFNLTLDKVCFWKKAGVVVAGTSQCPAELTTLVQDLQHLLSALRIRY
CHHHHHCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
DDTYPFTPHVTLVRNVRHCSLPDSIQAIDWPVTQWSLAQSRQTPYESVYQSLADWAL
CCCCCCCCCHHHHHCCHHCCCCCCCHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SGAAHPVRLFLALWPQDIARDQLAKLARQLADDCSGRCIQPKNLHLTLAFMGEIDQGNI
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCC
PRLCQAAPAIRQSPFNLTLDKVCFWKKAGVVVAGTSQCPAELTTLVQDLQHLLSALRIRY
CHHHHHCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
DDTYPFTPHVTLVRNVRHCSLPDSIQAIDWPVTQWSLAQSRQTPYESVYQSLADWAL
CCCCCCCCCHHHHHCCHHCCCCCCCHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]