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The map label for this gene is nadE [H]

Identifier: 113475647

GI number: 113475647

Start: 3093311

End: 3095065

Strand: Reverse

Name: nadE [H]

Synonym: Tery_1984

Alternate gene names: 113475647

Gene position: 3095065-3093311 (Counterclockwise)

Preceding gene: 113475648

Following gene: 113475646

Centisome position: 39.94

GC content: 32.88

Gene sequence:

>1755_bases
ATGAAAATTGCAATTGCTCAACTTAATCCTGTGATTGGTGATATTTCAGGCAATGCCAAATTAATTTTGGATGCTGCACA
AAAAGCAAAAAAATTAGATGCTAAGTTGATGATAACTCCAGAATTATCATTAATAGGTTATCCCCCACGAGATTTATTAA
TTTATCCTAGTTTAATTGAAGCTGCAGTTCTAGAATTAGAAAATTTAGCCAAATATTTACCATCAGAAATAGCCGTTTTA
GTAGGAACTGTAACCTTTAATTATCAAGCCGCTAATACAGGAGAAAAATCACTATTTAATAGTGCAGTTTTATTAACCAA
TGGCGAAATCAAACAAGTATTTCATAAACAACTTTTACCTACTTATGATGTATTTGATGAAGACCGATATTTTGAACCTG
GTAAAACTAGGGATTTTTTCACGTTAGAAAATTATTCTAACAGCTCAGAAAATTTAGCTAAAGTAGGTGTAACTATCTGC
GAAGACTTATGGAATGATGAGGCTTTTTGGGGAAAACGAAATTATGCTTATGACCCGATGAAAGAATTAGCAGCACAAAA
AGTTGATTTTGTGATTAATATGTCAGCTTCTCCCTATCAAACTGGAAAACAAAAATTGCGAGAAGCAATGTTAAAACATA
GTACAAATTGTTATCAAATACCAATTATTTATGTCAATCAAGTGGGTGGTAATGATGATTTAATTTTTGATGGTTGTAGT
GTAGTTTTTAATGGTGCTGGAAATGTGGTTTATCGTGCTCAAGCTTTTGAGACAAGTTTGGCAGTTGTAGAATTTAATTC
AGCAAAAAAAGATTTCATTTCAGTAGATTTTAAAAGTATAAATTTGCCAGAAAGTGAGGATGAAGAAATTTGGTCTGCTT
TGGTTTTAGGCCTAAGAGATTATGTACAAAAGTGTGGTTTTTCTAAAGTTGTTCTGGGTTTAAGTGGAGGAATAGACTCA
GCTTTAGTTGCAGCGATCGCTACTGCTGCATTAGGAAAAGAAAATGTCTTTGCTATTTTGATGCCTTCTCCCTACAGTTC
TGAGCATTCGGTAAAAGATGCTTTAGAATTAGCAGAAAATTTGGGTATTGCTAAACAAATTATATCTATTGAGAATTTAA
TGAAGGATTATGATAATAGTCTGTCAAGTTTATTTACAGGTACAAATTTTGGTATTGCTGAAGAGAATATTCAATCTCGG
ATTCGTGGAAATTTATTAATGGCTATTTCTAATAAGTTTGGTTATTTACTTTTATCTACAGGCAATAAGTCAGAAATGGC
TGTTGGTTATTGTACTCTTTATGGTGATATGAATGGTGGATTAGCAGTAATTTCAGATGTGCCGAAAACTCGGGTTTATT
CTTTATGTCAGTGGTTGAATGAACAGACAGTTAATAACAATAAAAAATTCTCTGGATCCCAAAACTTACTAATGACTGAA
AAGCAAAATATTATTCCCAAAAATATACTGACAAAAGCTCCCAGTGCTGAGTTAAAAGAAGGTCAAAAGGATGAGGATTC
TTTACCTGCTTATGAAGTCTTAGATGATATTTTATTTAGGTTAGTAGAAAAGTGCGAATCTTTAGACAAAATTATTGCTG
CAGGACATGATTTAGAGGTGGTAAATAAGGTAGTAAAATTAGTCATGAGGGCAGAATTTAAACGTAGACAAGCACCCCCA
GGTTTGAAAATTAGTACTCGCGCTTTTGGCACAGGTTGGCGGATGCCTATTGCTAAAAAATTAGTTATTAACTGA

Upstream 100 bases:

>100_bases
TATAAATTAATAATATGATAACATATTAATAAAATAAAAACGAATTTACAAAGTCGCCCTAGAAGAGCGATCGCTCAGAC
CAAACTTTTCAGTAAAAATT

Downstream 100 bases:

>100_bases
CTATTAGCACTCACAAATATTTTAAGTTTTAGTAGGCTTGGTGGTTTTAGGTTTTAAGAGCAAAGATAGAAAATGATTGA
AGTAAAAAGTTATGAAGATG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKIAIAQLNPVIGDISGNAKLILDAAQKAKKLDAKLMITPELSLIGYPPRDLLIYPSLIEAAVLELENLAKYLPSEIAVL
VGTVTFNYQAANTGEKSLFNSAVLLTNGEIKQVFHKQLLPTYDVFDEDRYFEPGKTRDFFTLENYSNSSENLAKVGVTIC
EDLWNDEAFWGKRNYAYDPMKELAAQKVDFVINMSASPYQTGKQKLREAMLKHSTNCYQIPIIYVNQVGGNDDLIFDGCS
VVFNGAGNVVYRAQAFETSLAVVEFNSAKKDFISVDFKSINLPESEDEEIWSALVLGLRDYVQKCGFSKVVLGLSGGIDS
ALVAAIATAALGKENVFAILMPSPYSSEHSVKDALELAENLGIAKQIISIENLMKDYDNSLSSLFTGTNFGIAEENIQSR
IRGNLLMAISNKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVISDVPKTRVYSLCQWLNEQTVNNNKKFSGSQNLLMTE
KQNIIPKNILTKAPSAELKEGQKDEDSLPAYEVLDDILFRLVEKCESLDKIIAAGHDLEVVNKVVKLVMRAEFKRRQAPP
GLKISTRAFGTGWRMPIAKKLVIN

Sequences:

>Translated_584_residues
MKIAIAQLNPVIGDISGNAKLILDAAQKAKKLDAKLMITPELSLIGYPPRDLLIYPSLIEAAVLELENLAKYLPSEIAVL
VGTVTFNYQAANTGEKSLFNSAVLLTNGEIKQVFHKQLLPTYDVFDEDRYFEPGKTRDFFTLENYSNSSENLAKVGVTIC
EDLWNDEAFWGKRNYAYDPMKELAAQKVDFVINMSASPYQTGKQKLREAMLKHSTNCYQIPIIYVNQVGGNDDLIFDGCS
VVFNGAGNVVYRAQAFETSLAVVEFNSAKKDFISVDFKSINLPESEDEEIWSALVLGLRDYVQKCGFSKVVLGLSGGIDS
ALVAAIATAALGKENVFAILMPSPYSSEHSVKDALELAENLGIAKQIISIENLMKDYDNSLSSLFTGTNFGIAEENIQSR
IRGNLLMAISNKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVISDVPKTRVYSLCQWLNEQTVNNNKKFSGSQNLLMTE
KQNIIPKNILTKAPSAELKEGQKDEDSLPAYEVLDDILFRLVEKCESLDKIIAAGHDLEVVNKVVKLVMRAEFKRRQAPP
GLKISTRAFGTGWRMPIAKKLVIN
>Mature_584_residues
MKIAIAQLNPVIGDISGNAKLILDAAQKAKKLDAKLMITPELSLIGYPPRDLLIYPSLIEAAVLELENLAKYLPSEIAVL
VGTVTFNYQAANTGEKSLFNSAVLLTNGEIKQVFHKQLLPTYDVFDEDRYFEPGKTRDFFTLENYSNSSENLAKVGVTIC
EDLWNDEAFWGKRNYAYDPMKELAAQKVDFVINMSASPYQTGKQKLREAMLKHSTNCYQIPIIYVNQVGGNDDLIFDGCS
VVFNGAGNVVYRAQAFETSLAVVEFNSAKKDFISVDFKSINLPESEDEEIWSALVLGLRDYVQKCGFSKVVLGLSGGIDS
ALVAAIATAALGKENVFAILMPSPYSSEHSVKDALELAENLGIAKQIISIENLMKDYDNSLSSLFTGTNFGIAEENIQSR
IRGNLLMAISNKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVISDVPKTRVYSLCQWLNEQTVNNNKKFSGSQNLLMTE
KQNIIPKNILTKAPSAELKEGQKDEDSLPAYEVLDDILFRLVEKCESLDKIIAAGHDLEVVNKVVKLVMRAEFKRRQAPP
GLKISTRAFGTGWRMPIAKKLVIN

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 64514; Mature: 64514

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAQLNPVIGDISGNAKLILDAAQKAKKLDAKLMITPELSLIGYPPRDLLIYPSLIE
CEEEEEECCCEEEECCCCCEEEEEHHHHHHHCCCEEEEECCCEEECCCCHHEEECHHHHH
AAVLELENLAKYLPSEIAVLVGTVTFNYQAANTGEKSLFNSAVLLTNGEIKQVFHKQLLP
HHHHHHHHHHHHCCHHHEEEEEEEEEEEEECCCCCHHHHCCEEEEECCHHHHHHHHHCCC
TYDVFDEDRYFEPGKTRDFFTLENYSNSSENLAKVGVTICEDLWNDEAFWGKRNYAYDPM
CCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
KELAAQKVDFVINMSASPYQTGKQKLREAMLKHSTNCYQIPIIYVNQVGGNDDLIFDGCS
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEECCE
VVFNGAGNVVYRAQAFETSLAVVEFNSAKKDFISVDFKSINLPESEDEEIWSALVLGLRD
EEECCCCCEEEEEEHHCCEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
YVQKCGFSKVVLGLSGGIDSALVAAIATAALGKENVFAILMPSPYSSEHSVKDALELAEN
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
LGIAKQIISIENLMKDYDNSLSSLFTGTNFGIAEENIQSRIRGNLLMAISNKFGYLLLST
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEC
GNKSEMAVGYCTLYGDMNGGLAVISDVPKTRVYSLCQWLNEQTVNNNKKFSGSQNLLMTE
CCCCCEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
KQNIIPKNILTKAPSAELKEGQKDEDSLPAYEVLDDILFRLVEKCESLDKIIAAGHDLEV
CCCCCCHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VNKVVKLVMRAEFKRRQAPPGLKISTRAFGTGWRMPIAKKLVIN
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKIAIAQLNPVIGDISGNAKLILDAAQKAKKLDAKLMITPELSLIGYPPRDLLIYPSLIE
CEEEEEECCCEEEECCCCCEEEEEHHHHHHHCCCEEEEECCCEEECCCCHHEEECHHHHH
AAVLELENLAKYLPSEIAVLVGTVTFNYQAANTGEKSLFNSAVLLTNGEIKQVFHKQLLP
HHHHHHHHHHHHCCHHHEEEEEEEEEEEEECCCCCHHHHCCEEEEECCHHHHHHHHHCCC
TYDVFDEDRYFEPGKTRDFFTLENYSNSSENLAKVGVTICEDLWNDEAFWGKRNYAYDPM
CCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
KELAAQKVDFVINMSASPYQTGKQKLREAMLKHSTNCYQIPIIYVNQVGGNDDLIFDGCS
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEECCE
VVFNGAGNVVYRAQAFETSLAVVEFNSAKKDFISVDFKSINLPESEDEEIWSALVLGLRD
EEECCCCCEEEEEEHHCCEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
YVQKCGFSKVVLGLSGGIDSALVAAIATAALGKENVFAILMPSPYSSEHSVKDALELAEN
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
LGIAKQIISIENLMKDYDNSLSSLFTGTNFGIAEENIQSRIRGNLLMAISNKFGYLLLST
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEC
GNKSEMAVGYCTLYGDMNGGLAVISDVPKTRVYSLCQWLNEQTVNNNKKFSGSQNLLMTE
CCCCCEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
KQNIIPKNILTKAPSAELKEGQKDEDSLPAYEVLDDILFRLVEKCESLDKIIAAGHDLEV
CCCCCCHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VNKVVKLVMRAEFKRRQAPPGLKISTRAFGTGWRMPIAKKLVIN
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]