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The map label for this gene is miaB

Identifier: 113474257

GI number: 113474257

Start: 581688

End: 583043

Strand: Direct

Name: miaB

Synonym: Tery_0377

Alternate gene names: 113474257

Gene position: 581688-583043 (Clockwise)

Preceding gene: 113474249

Following gene: 113474258

Centisome position: 7.51

GC content: 41.74

Gene sequence:

>1356_bases
ATGAATACAAATAACCGTCACTATCACATTACTACTTTCGGCTGTCAAATGAACAAAGCTGACTCCGAACGCATGGCAGG
TATCTTAGATAATATGGGTCTTATTTCTTCAGAAGACCCTAACAAAGCTGATATCATTCTATATAATACTTGCACTATTC
GCGACAATGCTGAACAAAAAGTCTATTCTTACCTGGGTAGACAAGCAAAACGTAAACATAAACAACCAGACCTCACTCTT
ATTGTTGCTGGATGTGTGGCTCAACAGGAAGGGGCGGCACTACTGCGGCGAGTTCCCGAACTTGACTTAATTATGGGACC
TCAACATGCTAACCGTTTGCAAGATTTATTAGAACAGGTATTTAATGGCAACCAAGTAGTCGCGACTGAACCTATTCATA
TTGTGGAAGATATTACTAAACCTAGACGGGATAGTAAAATTACTGCTTGGGTAAATATAATTTATGGTTGCAACGAACAC
TGTACCTATTGTGTTGTTCCTAGTGTACGGGGTGTAGAGCAGTCTCGTACACCTGAGGCTATTCGCGCTGAAATGGAAGA
GTTGGGGCGACAAGGTTATCAGGAAATAACATTGTTAGGTCAAAATATTGATGCTTATGGACGGGACTTACCCGGGGTAA
CAAAAGAAGGGCGTAATAAATACACTTTCACAGATTTACTTTACTATGTGCATGATGTACCAGGTGTTGAACGTATTCGC
TTTGCCACGAGTCACCCCCGCTATTTTACAGAACGTTTAATTAGAGCTTGTGCTGAGTTGCCTAAGGTTTGTGAACATTT
CCATATTCCGTTTCAGTCTGGAGATAATAAGTTATTGAAGGCAATGGCTAGGGGTTACACTCATGAAAAATATCGCCGCA
TTATTGATAAAATTCGGGAACTGATGCCGGATGCTTCTATTAGTGCTGATGCAATAGTTGGGTTTCCTGGGGAAACTGAG
GCACAATTTGAGAATACTCTGAAGTTGGTGGAGGATATTGGGTTTGATCAGTTGAATACTGCTGCTTATTCTCCTAGGCC
GGGAACTCCAGCGGCTTTATGGGAAAATCAATTGGGTGAGGAGGTGAAGTCAGATAGACTACAACGGTTGAATCATTTGG
TTGGTGTGAAGGCGGCCGATCGTTCGCAACGTTATATGGGTCGTATTGAAGAAGTTTTAGTTGAAGACATGAACCCTAAA
AATGCTGCTCAAGTTATGGGTCGTACCCGTGGTAACCGTCTGACATTTTTTGAGGGAGATATTGCTCAGTTGAAGGGTAA
GTTAGTTAAGGTGAAAATTACTGAAGTACGTCCATTTAGTTTGACTGGTGAGGTGAAGGAATTTGTGCTAGTCTAG

Upstream 100 bases:

>100_bases
GGGGGCAAATGCCATTTACTCCTACTAGATATGGCGTTTGGTGCGTAAGTCCTGATAATATTAAGTTCTGGTATACTATC
ATTTTATTCCATCTTTAAAT

Downstream 100 bases:

>100_bases
GAGTTTTGACTTATAACGTTTTCATATTAATGAACCATATCGTCATAAGCAAAACCAAGCTGGAAAATTGTTGTAGCCTG
CGTATGAGTAATATCATGTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTNNRHYHITTFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQKVYSYLGRQAKRKHKQPDLTL
IVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQVFNGNQVVATEPIHIVEDITKPRRDSKITAWVNIIYGCNEH
CTYCVVPSVRGVEQSRTPEAIRAEMEELGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIR
FATSHPRYFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRELMPDASISADAIVGFPGETE
AQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGEEVKSDRLQRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPK
NAAQVMGRTRGNRLTFFEGDIAQLKGKLVKVKITEVRPFSLTGEVKEFVLV

Sequences:

>Translated_451_residues
MNTNNRHYHITTFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQKVYSYLGRQAKRKHKQPDLTL
IVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQVFNGNQVVATEPIHIVEDITKPRRDSKITAWVNIIYGCNEH
CTYCVVPSVRGVEQSRTPEAIRAEMEELGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIR
FATSHPRYFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRELMPDASISADAIVGFPGETE
AQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGEEVKSDRLQRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPK
NAAQVMGRTRGNRLTFFEGDIAQLKGKLVKVKITEVRPFSLTGEVKEFVLV
>Mature_451_residues
MNTNNRHYHITTFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQKVYSYLGRQAKRKHKQPDLTL
IVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQVFNGNQVVATEPIHIVEDITKPRRDSKITAWVNIIYGCNEH
CTYCVVPSVRGVEQSRTPEAIRAEMEELGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIR
FATSHPRYFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRELMPDASISADAIVGFPGETE
AQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGEEVKSDRLQRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPK
NAAQVMGRTRGNRLTFFEGDIAQLKGKLVKVKITEVRPFSLTGEVKEFVLV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=31.5789473684211, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=32.3529411764706, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI93277076, Length=379, Percent_Identity=31.1345646437995, Blast_Score=165, Evalue=7e-41,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=41.2556053811659, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=27.2930648769575, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=32.7659574468085, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=387, Percent_Identity=26.0981912144703, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356207, Length=438, Percent_Identity=33.3333333333333, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=30.1724137931034, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_TRIEI (Q119H9)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_720318.1
- ProteinModelPortal:   Q119H9
- SMR:   Q119H9
- STRING:   Q119H9
- GeneID:   4241611
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_0377
- NMPDR:   fig|203124.1.peg.2421
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q119H9
- ProtClustDB:   PRK14333
- BioCyc:   TERY203124:TERY_0377-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51118; Mature: 51118

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNNRHYHITTFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQK
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHKQPDLTLIVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQV
HHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FNGNQVVATEPIHIVEDITKPRRDSKITAWVNIIYGCNEHCTYCVVPSVRGVEQSRTPEA
HCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCHHH
IRAEMEELGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIR
HHHHHHHHHHCCHHEEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
LMPDASISADAIVGFPGETEAQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
EVKSDRLQRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPKNAAQVMGRTRGNRLTFFEGD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCC
IAQLKGKLVKVKITEVRPFSLTGEVKEFVLV
HHHHCCEEEEEEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MNTNNRHYHITTFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQK
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHKQPDLTLIVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQV
HHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FNGNQVVATEPIHIVEDITKPRRDSKITAWVNIIYGCNEHCTYCVVPSVRGVEQSRTPEA
HCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCHHH
IRAEMEELGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIR
HHHHHHHHHHCCHHEEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
LMPDASISADAIVGFPGETEAQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
EVKSDRLQRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPKNAAQVMGRTRGNRLTFFEGD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCC
IAQLKGKLVKVKITEVRPFSLTGEVKEFVLV
HHHHCCEEEEEEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA