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The map label for this gene is amtB3 [C]

Identifier: 110835390

GI number: 110835390

Start: 2868143

End: 2869390

Strand: Reverse

Name: amtB3 [C]

Synonym: ABO_2529

Alternate gene names: 110835390

Gene position: 2869390-2868143 (Counterclockwise)

Preceding gene: 110835391

Following gene: 110835389

Centisome position: 91.96

GC content: 55.53

Gene sequence:

>1248_bases
GTGGAAAACCAAATTTTTGAATTGAAATACGCGCTGGATACGTTTTATTTCCTTATCTGTGGCGCGCTAGTTATGTGGAT
GGCTGCCGGCTTTGCCATGCTCGAAGCGGGCCTGGTGCGCGCCAAGAACACCACGGAAATTCTCACCAAAAACGTGGCCC
TGTTTGCCATCGCCAGCATCATGTACATGGTGTGCGGTTACGCGATCATGTATGACGGTGCTATTTTCCTTTCTGGGATT
GTGGGCGACGGGGTTGCTGGCGCAGAAGAAGCGGCTACCTACGCACCATCTGCTGATTTCTTCTTCCAGGTTGTGTTTGT
CGCAACGGCCATGTCCATCGTATCTGGTGCGGTGGCAGAACGGATGAAGTTGTGGGCATTCTTGATCTTCGCGGTTGTTA
TGACCGGCATTATCTATCCGCTGGAAGGCTCCTGGACGTGGGGTGGTGCGGATGTGTTCGGTTTGTATAACTTGGGCGAC
CAGGGCTTCTCTGACTTCGCCGGTTCCGGCATCGTTCACATGGCGGGTGCGGCTGCTGCCTTGGCTGGTGTTCTGCTGTT
GGGCGCACGAAAAGGCAAATATGGCCCGAACGGTGAAGTACACGCCATCCCGGGTTCTAACCTGCCATTGGCCGCACTGG
GTACCTTCATCCTGTGGTTGGGCTGGTTCGGTTTCAATGGTGGCTCTGTGCTGGCAACGTCCAGTGTCGGTGACTCCAAC
GCGGTGGCGGTTGTGTTCATGAACACTAACGCGGCTGCTGCCGGTGGTGCCATTGCTGCGCTGATTGTTGGTCGCATCTT
GTTCGGCAAAGCCGACCTGACCATGCTGCTTAACGGCGCCTTGGCAGGGCTGGTGGCGATCACTGCGGAGCCCTCTACCC
CGACTGCTTTGCAGGCAACCCTGTTCGGTGCCTTGGGTGGCGTGCTGGTTGTATTTAGCATCCTGACTCTGGACAAGATG
AAGATCGATGATCCGGTGGGTGCGATCTCCGTGCATGGTGTAGTTGGTTTCCTCGGCTTGATGCTGGTTCCGATCACCAA
CCCGCTGACCGACGATGGTGGCGCAAGCTTCTCTGGTCAGCTGATCGGTGCAGTGACTATCTTTGCTTGGGTGTTCGTTG
CAAGCCTGGTGGTATGGGGCATCCTGAAAGTGGTAATGGGTATCCGCGTTAGCGAGGAAGAAGAGTACGAAGGCATGGAT
ATCGCGGACTGCGGTATGGAAGCCTACCCTGAGTTTGTTAAGGGTTAA

Upstream 100 bases:

>100_bases
GGTGAAACCGGCGAGGACGCGATTTAAGTTTTACCGTGTTTTGAATTGACCCATGCCAGGGCTTTAAGAGAACACGCTTC
CATTTTCGGAGGGTTGCATT

Downstream 100 bases:

>100_bases
TAAGAGGCGCAGCAAGCAACACGCGTCATGCAACAAAAAAGCCCCGGCCGGTTGGTCGGGGCTTTTTTGTGAGTGGCGGA
TTTTTGCGTTTTCTACAATG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MENQIFELKYALDTFYFLICGALVMWMAAGFAMLEAGLVRAKNTTEILTKNVALFAIASIMYMVCGYAIMYDGAIFLSGI
VGDGVAGAEEAATYAPSADFFFQVVFVATAMSIVSGAVAERMKLWAFLIFAVVMTGIIYPLEGSWTWGGADVFGLYNLGD
QGFSDFAGSGIVHMAGAAAALAGVLLLGARKGKYGPNGEVHAIPGSNLPLAALGTFILWLGWFGFNGGSVLATSSVGDSN
AVAVVFMNTNAAAAGGAIAALIVGRILFGKADLTMLLNGALAGLVAITAEPSTPTALQATLFGALGGVLVVFSILTLDKM
KIDDPVGAISVHGVVGFLGLMLVPITNPLTDDGGASFSGQLIGAVTIFAWVFVASLVVWGILKVVMGIRVSEEEEYEGMD
IADCGMEAYPEFVKG

Sequences:

>Translated_415_residues
MENQIFELKYALDTFYFLICGALVMWMAAGFAMLEAGLVRAKNTTEILTKNVALFAIASIMYMVCGYAIMYDGAIFLSGI
VGDGVAGAEEAATYAPSADFFFQVVFVATAMSIVSGAVAERMKLWAFLIFAVVMTGIIYPLEGSWTWGGADVFGLYNLGD
QGFSDFAGSGIVHMAGAAAALAGVLLLGARKGKYGPNGEVHAIPGSNLPLAALGTFILWLGWFGFNGGSVLATSSVGDSN
AVAVVFMNTNAAAAGGAIAALIVGRILFGKADLTMLLNGALAGLVAITAEPSTPTALQATLFGALGGVLVVFSILTLDKM
KIDDPVGAISVHGVVGFLGLMLVPITNPLTDDGGASFSGQLIGAVTIFAWVFVASLVVWGILKVVMGIRVSEEEEYEGMD
IADCGMEAYPEFVKG
>Mature_415_residues
MENQIFELKYALDTFYFLICGALVMWMAAGFAMLEAGLVRAKNTTEILTKNVALFAIASIMYMVCGYAIMYDGAIFLSGI
VGDGVAGAEEAATYAPSADFFFQVVFVATAMSIVSGAVAERMKLWAFLIFAVVMTGIIYPLEGSWTWGGADVFGLYNLGD
QGFSDFAGSGIVHMAGAAAALAGVLLLGARKGKYGPNGEVHAIPGSNLPLAALGTFILWLGWFGFNGGSVLATSSVGDSN
AVAVVFMNTNAAAAGGAIAALIVGRILFGKADLTMLLNGALAGLVAITAEPSTPTALQATLFGALGGVLVVFSILTLDKM
KIDDPVGAISVHGVVGFLGLMLVPITNPLTDDGGASFSGQLIGAVTIFAWVFVASLVVWGILKVVMGIRVSEEEEYEGMD
IADCGMEAYPEFVKG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=33.2530120481928, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17550180, Length=430, Percent_Identity=36.5116279069767, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17550182, Length=430, Percent_Identity=33.2558139534884, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI32564042, Length=437, Percent_Identity=28.3752860411899, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI133931100, Length=349, Percent_Identity=29.7994269340974, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=26.5446224256293, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324187, Length=414, Percent_Identity=25.8454106280193, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321559, Length=425, Percent_Identity=26.3529411764706, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=31.4903846153846, Blast_Score=164, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43057; Mature: 43057

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQIFELKYALDTFYFLICGALVMWMAAGFAMLEAGLVRAKNTTEILTKNVALFAIASI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYMVCGYAIMYDGAIFLSGIVGDGVAGAEEAATYAPSADFFFQVVFVATAMSIVSGAVAE
HHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RMKLWAFLIFAVVMTGIIYPLEGSWTWGGADVFGLYNLGDQGFSDFAGSGIVHMAGAAAA
HHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHH
LAGVLLLGARKGKYGPNGEVHAIPGSNLPLAALGTFILWLGWFGFNGGSVLATSSVGDSN
HHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AVAVVFMNTNAAAAGGAIAALIVGRILFGKADLTMLLNGALAGLVAITAEPSTPTALQAT
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHH
LFGALGGVLVVFSILTLDKMKIDDPVGAISVHGVVGFLGLMLVPITNPLTDDGGASFSGQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
LIGAVTIFAWVFVASLVVWGILKVVMGIRVSEEEEYEGMDIADCGMEAYPEFVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCC
>Mature Secondary Structure
MENQIFELKYALDTFYFLICGALVMWMAAGFAMLEAGLVRAKNTTEILTKNVALFAIASI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYMVCGYAIMYDGAIFLSGIVGDGVAGAEEAATYAPSADFFFQVVFVATAMSIVSGAVAE
HHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RMKLWAFLIFAVVMTGIIYPLEGSWTWGGADVFGLYNLGDQGFSDFAGSGIVHMAGAAAA
HHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHH
LAGVLLLGARKGKYGPNGEVHAIPGSNLPLAALGTFILWLGWFGFNGGSVLATSSVGDSN
HHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AVAVVFMNTNAAAAGGAIAALIVGRILFGKADLTMLLNGALAGLVAITAEPSTPTALQAT
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHH
LFGALGGVLVVFSILTLDKMKIDDPVGAISVHGVVGFLGLMLVPITNPLTDDGGASFSGQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
LIGAVTIFAWVFVASLVVWGILKVVMGIRVSEEEEYEGMDIADCGMEAYPEFVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]