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The map label for this gene is rlmI [H]

Identifier: 110835389

GI number: 110835389

Start: 2866720

End: 2867925

Strand: Reverse

Name: rlmI [H]

Synonym: ABO_2528

Alternate gene names: 110835389

Gene position: 2867925-2866720 (Counterclockwise)

Preceding gene: 110835390

Following gene: 110835388

Centisome position: 91.92

GC content: 54.48

Gene sequence:

>1206_bases
GTGTGCACCATGACTGAACCAACCGTTATTCGCCTGCGTAAAAATGAAGAACGTCGCCTGAAAGCGGGCCACTTGTGGGT
TTATGCCAACGAGATTGATACCCGACATACCCCGCTTAAGGATTTGTCTGCCGGTACTTGTGTACTGGAAGATAGCCGTG
GCAAACCGTTGGGGCGCGCCCTGCTTAGCCCGCATTCATTGATTGCGGCGCGTCTGTATAGCCGTGATGTGAAGCAGGCG
CTGTCGCGTAGCTTTTTCAAAAAGCGTATTGAGCAAGCGCTGACCTTGCGGGAACAACTGTTTGACGATGCCTGCTACCG
GTTGATTTACGGCGAAGCAGATGGTCTACCCGGCCTAGTGGTGGATCGCTTCAACGATGTGCTGGTGGTGCAGACCGGTT
CCGGTGCCATGGAAGCCCACCTTGACGTGATTGTTAGTGCCCTGGATGCGGTGCTGGCGCCAACCCATATTATCGCCAAG
AACGAAGCCGTTGCCCGTGAATTTGAAGGCTTAGACCGTTATACCAAGGCGTTGAAAGGCGAGTTGTTGGATGAAATCAG
CCTCACTGAAAATGGTGCGGCTTTTGTGGCGCCCTTAGCGGAAGGGCAAAAGACCGGCTGGTTCTATGACCACCGAGCTG
CTCGCCAGAAAATGCAGGCGCTGGCCAAAGGTGCTCGTGTGTTGGATGTGTTCAGTTATTGTGGCGGTTGGGGCGTGCAA
GCGGCATTGGCCGGGGCGGAATCGGTGACGTGTGTGGATGGTTCCGAAATCGCCACGGATTATGTGCACCGTAATGCGCA
GCTGAACGGTGTTGCCGATACCGTGGGGAGCATTCAGGGCGATGCTTTTGCGGCTATGAAGCAATTGATCGCTGACGGTG
AAAAATACGATTTGGTAGTTCTGGATCCGCCAGCGTTTATCAAGCGCAAGAAAGATTTCAAGAACGGCTTGGCCGGTTAC
CACGCCATTAATGAGCTGGCCATGCGATTAGTAAAGCCGGGTGGGTACCTGGTATCAGCCTCTTGTTCTATGCATATGCC
TGCAGAGCGCTTGCTTGAGGTGGTGCATAGCAGCGCTCGCCATATTGACCGCAGTATTCAGGTGGTGGGTTATGAGGGGC
AAGGGCCGGACCATCCTGTGCACCCGGCCATTGCAGAAACCGCCTACCTGAAAACCTGGTTCTGCCGCGTGTTGTTCAGT
CTTTAG

Upstream 100 bases:

>100_bases
TGCCAGTGCCGCTTTGTGTTTTTGTGCGCAGCACTGCGCAGTGGTTGACCGTGCCATTCCTCCCCTATGCTATCATCGCT
CCCTTTCCGGCCAGCAGTAT

Downstream 100 bases:

>100_bases
CCGTCTCTACGGCTGGATTTCGCGCGCTGTTCCTGAAAGTCACGGCGTTGATGGTTGGGTATGATGATCTGAGCCCGTCA
CAGGCCAGCACCCAGTGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MCTMTEPTVIRLRKNEERRLKAGHLWVYANEIDTRHTPLKDLSAGTCVLEDSRGKPLGRALLSPHSLIAARLYSRDVKQA
LSRSFFKKRIEQALTLREQLFDDACYRLIYGEADGLPGLVVDRFNDVLVVQTGSGAMEAHLDVIVSALDAVLAPTHIIAK
NEAVAREFEGLDRYTKALKGELLDEISLTENGAAFVAPLAEGQKTGWFYDHRAARQKMQALAKGARVLDVFSYCGGWGVQ
AALAGAESVTCVDGSEIATDYVHRNAQLNGVADTVGSIQGDAFAAMKQLIADGEKYDLVVLDPPAFIKRKKDFKNGLAGY
HAINELAMRLVKPGGYLVSASCSMHMPAERLLEVVHSSARHIDRSIQVVGYEGQGPDHPVHPAIAETAYLKTWFCRVLFS
L

Sequences:

>Translated_401_residues
MCTMTEPTVIRLRKNEERRLKAGHLWVYANEIDTRHTPLKDLSAGTCVLEDSRGKPLGRALLSPHSLIAARLYSRDVKQA
LSRSFFKKRIEQALTLREQLFDDACYRLIYGEADGLPGLVVDRFNDVLVVQTGSGAMEAHLDVIVSALDAVLAPTHIIAK
NEAVAREFEGLDRYTKALKGELLDEISLTENGAAFVAPLAEGQKTGWFYDHRAARQKMQALAKGARVLDVFSYCGGWGVQ
AALAGAESVTCVDGSEIATDYVHRNAQLNGVADTVGSIQGDAFAAMKQLIADGEKYDLVVLDPPAFIKRKKDFKNGLAGY
HAINELAMRLVKPGGYLVSASCSMHMPAERLLEVVHSSARHIDRSIQVVGYEGQGPDHPVHPAIAETAYLKTWFCRVLFS
L
>Mature_401_residues
MCTMTEPTVIRLRKNEERRLKAGHLWVYANEIDTRHTPLKDLSAGTCVLEDSRGKPLGRALLSPHSLIAARLYSRDVKQA
LSRSFFKKRIEQALTLREQLFDDACYRLIYGEADGLPGLVVDRFNDVLVVQTGSGAMEAHLDVIVSALDAVLAPTHIIAK
NEAVAREFEGLDRYTKALKGELLDEISLTENGAAFVAPLAEGQKTGWFYDHRAARQKMQALAKGARVLDVFSYCGGWGVQ
AALAGAESVTCVDGSEIATDYVHRNAQLNGVADTVGSIQGDAFAAMKQLIADGEKYDLVVLDPPAFIKRKKDFKNGLAGY
HAINELAMRLVKPGGYLVSASCSMHMPAERLLEVVHSSARHIDRSIQVVGYEGQGPDHPVHPAIAETAYLKTWFCRVLFS
L

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=32.6530612244898, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1787181, Length=249, Percent_Identity=32.9317269076305, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44007; Mature: 44007

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTMTEPTVIRLRKNEERRLKAGHLWVYANEIDTRHTPLKDLSAGTCVLEDSRGKPLGRA
CCCCCCCEEEEEECCHHHHEECCEEEEEECCCCCCCCCHHHCCCCEEEEECCCCCHHHHH
LLSPHSLIAARLYSRDVKQALSRSFFKKRIEQALTLREQLFDDACYRLIYGEADGLPGLV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VDRFNDVLVVQTGSGAMEAHLDVIVSALDAVLAPTHIIAKNEAVAREFEGLDRYTKALKG
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHH
ELLDEISLTENGAAFVAPLAEGQKTGWFYDHRAARQKMQALAKGARVLDVFSYCGGWGVQ
HHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHH
AALAGAESVTCVDGSEIATDYVHRNAQLNGVADTVGSIQGDAFAAMKQLIADGEKYDLVV
HHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEE
LDPPAFIKRKKDFKNGLAGYHAINELAMRLVKPGGYLVSASCSMHMPAERLLEVVHSSAR
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
HIDRSIQVVGYEGQGPDHPVHPAIAETAYLKTWFCRVLFSL
HHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCTMTEPTVIRLRKNEERRLKAGHLWVYANEIDTRHTPLKDLSAGTCVLEDSRGKPLGRA
CCCCCCCEEEEEECCHHHHEECCEEEEEECCCCCCCCCHHHCCCCEEEEECCCCCHHHHH
LLSPHSLIAARLYSRDVKQALSRSFFKKRIEQALTLREQLFDDACYRLIYGEADGLPGLV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VDRFNDVLVVQTGSGAMEAHLDVIVSALDAVLAPTHIIAKNEAVAREFEGLDRYTKALKG
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHH
ELLDEISLTENGAAFVAPLAEGQKTGWFYDHRAARQKMQALAKGARVLDVFSYCGGWGVQ
HHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHH
AALAGAESVTCVDGSEIATDYVHRNAQLNGVADTVGSIQGDAFAAMKQLIADGEKYDLVV
HHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEE
LDPPAFIKRKKDFKNGLAGYHAINELAMRLVKPGGYLVSASCSMHMPAERLLEVVHSSAR
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
HIDRSIQVVGYEGQGPDHPVHPAIAETAYLKTWFCRVLFSL
HHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA