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The map label for this gene is nylA [H]

Identifier: 110835343

GI number: 110835343

Start: 2814718

End: 2816214

Strand: Reverse

Name: nylA [H]

Synonym: ABO_2482

Alternate gene names: 110835343

Gene position: 2816214-2814718 (Counterclockwise)

Preceding gene: 110835345

Following gene: 110835342

Centisome position: 90.26

GC content: 59.12

Gene sequence:

>1497_bases
ATGAAGTTTGCAGAATACCGCCAGTTTGATGCCATCGGGCTTGCCGAGCTGGTGGCGCGGGGGGAGGTCACGGCAATGGA
ATTGCTGGAGCTGGCGATTGCCCGTGCCGATGCGGTGAACCCGCAATTAAATGGTTTGATTATTCCATTGTATGACATGG
CCCGAAAACGCGCCGCGGGGCCATTGAGCGGACCGTTGGCGGGTGTGCCCATGCTGGTAAAAGATCTTTTTCAGGAAATC
GGCGGTGCGCCCAACTATCAGGGCAATAAAGCGCGCAAGGCTCGTGATGTCCATGCGCAAAGAGATTCTACCCTGGTGGC
ACGCTGGAAACAGGGCGGGCTGGTACCATTCGGCCGCACCAATACCCCGGAGTTTGGCGCCAAGGGCGTTACCGAGCCGG
ATTCGTTCGGGCCGGCGCGTAATCCCTGGAACGTAGATCACACTCCCGGTGGTTCGTCCGGGGGCTCGGCGGCTATGGTG
GCTGCAGGTGTGGTACCGGTGGCCGGCGCTAATGATGGTGGCGGTAGTATCCGGATTCCCGCTGCCTGTTGTGGTCTGTT
CGGGCTCAAGCCGGGCCGTGGGCGCACCCCCTGGGGGCCCGAATTCACTGAGGCCATGCATGGAATAGCGGTAAACCACG
CGCTGACCCGCAGCGTGCGTGACAGTGCGTTGCTGTTGGATATTACCCAGGGTGACGAGCGCGGTAGTCTGTTTCAGATT
GCTCCGCCGGAGCAAGCTTACGCCCTGTCTGCGGCTAGCCGCCCCGGCAAGCTGCGTATCGGTTTTTCTACTCGCTCGCC
GCTAGGCACCGATGTGGATTCGGAGGCGATCCGGGCGGTGGAGAAAACCGTGGCGTTGTTACGGGACTTGGGCCACGAAG
TGGAAGAGGCTGAGCCACAGATGGACGCCAAACAGATGTGCATGGATTGGCTATCGGTTTGGTTTGGCCAGTGTGCTGCC
GAGGTGGATGAGGTGAAAACTATGACCGGTTGCGGTGATGAGGGCTTTGAGCTGGATACCTTGGCCATTGCCGCTTTTGG
CCGTGCTACCCCGGCCCATGAATATGTGAAGTGCCAGACCCGTTGGCAGTTATACATGATCGCCATGGATACCTTCTTGG
AGCAGTACGATTTCTGGTTGACCCCTACTCTGGCGTCCCCCCCTGTCGGTATCGGCGCTATGGCGACACCGCCGTGGCAG
CAGACCGCTCTGAAGGTGTTGCTCAAGGTCGGCGGGGAAAAGCTGTTAATGAAGACCGGCATGATTGAGCAGGTGGCCAT
AGAGAACTTCAAATACATGCCGTTCACCCAGTTTGCCAATGTCATTGGTGTGCCTGCCATGTCGGTGCCACTGCACTGGT
GTGATTCTTCTTCAGGAAACGTGGGCCTGCCGCTGGGTTCCCAGTTTGTTGGTGGCCATGGGGATGAAGGCAAGCTGCTG
GCGCTGGCGGCGCAACTGGAAGAGGCGGCTCCGTGGTTCAATAAGGTGCCGATGTAA

Upstream 100 bases:

>100_bases
TGATTAAGCATACTTGGCTGCGCAGACTATGCCACAGTATGGATTGATCGTCTGGTAGCAGGCCATTCCTGCGCTTCGGG
ATAGTGAAAGGGAGAGCACA

Downstream 100 bases:

>100_bases
TGCCCTGTCGGAACGTATCGCAAGAGATGGTTCGCAAGGTTCCCGAATGTTGCTCTCCTCGTTGGTCAACAATCAGAGGA
AAGCTTACAAACATCACAAA

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKFAEYRQFDAIGLAELVARGEVTAMELLELAIARADAVNPQLNGLIIPLYDMARKRAAGPLSGPLAGVPMLVKDLFQEI
GGAPNYQGNKARKARDVHAQRDSTLVARWKQGGLVPFGRTNTPEFGAKGVTEPDSFGPARNPWNVDHTPGGSSGGSAAMV
AAGVVPVAGANDGGGSIRIPAACCGLFGLKPGRGRTPWGPEFTEAMHGIAVNHALTRSVRDSALLLDITQGDERGSLFQI
APPEQAYALSAASRPGKLRIGFSTRSPLGTDVDSEAIRAVEKTVALLRDLGHEVEEAEPQMDAKQMCMDWLSVWFGQCAA
EVDEVKTMTGCGDEGFELDTLAIAAFGRATPAHEYVKCQTRWQLYMIAMDTFLEQYDFWLTPTLASPPVGIGAMATPPWQ
QTALKVLLKVGGEKLLMKTGMIEQVAIENFKYMPFTQFANVIGVPAMSVPLHWCDSSSGNVGLPLGSQFVGGHGDEGKLL
ALAAQLEEAAPWFNKVPM

Sequences:

>Translated_498_residues
MKFAEYRQFDAIGLAELVARGEVTAMELLELAIARADAVNPQLNGLIIPLYDMARKRAAGPLSGPLAGVPMLVKDLFQEI
GGAPNYQGNKARKARDVHAQRDSTLVARWKQGGLVPFGRTNTPEFGAKGVTEPDSFGPARNPWNVDHTPGGSSGGSAAMV
AAGVVPVAGANDGGGSIRIPAACCGLFGLKPGRGRTPWGPEFTEAMHGIAVNHALTRSVRDSALLLDITQGDERGSLFQI
APPEQAYALSAASRPGKLRIGFSTRSPLGTDVDSEAIRAVEKTVALLRDLGHEVEEAEPQMDAKQMCMDWLSVWFGQCAA
EVDEVKTMTGCGDEGFELDTLAIAAFGRATPAHEYVKCQTRWQLYMIAMDTFLEQYDFWLTPTLASPPVGIGAMATPPWQ
QTALKVLLKVGGEKLLMKTGMIEQVAIENFKYMPFTQFANVIGVPAMSVPLHWCDSSSGNVGLPLGSQFVGGHGDEGKLL
ALAAQLEEAAPWFNKVPM
>Mature_498_residues
MKFAEYRQFDAIGLAELVARGEVTAMELLELAIARADAVNPQLNGLIIPLYDMARKRAAGPLSGPLAGVPMLVKDLFQEI
GGAPNYQGNKARKARDVHAQRDSTLVARWKQGGLVPFGRTNTPEFGAKGVTEPDSFGPARNPWNVDHTPGGSSGGSAAMV
AAGVVPVAGANDGGGSIRIPAACCGLFGLKPGRGRTPWGPEFTEAMHGIAVNHALTRSVRDSALLLDITQGDERGSLFQI
APPEQAYALSAASRPGKLRIGFSTRSPLGTDVDSEAIRAVEKTVALLRDLGHEVEEAEPQMDAKQMCMDWLSVWFGQCAA
EVDEVKTMTGCGDEGFELDTLAIAAFGRATPAHEYVKCQTRWQLYMIAMDTFLEQYDFWLTPTLASPPVGIGAMATPPWQ
QTALKVLLKVGGEKLLMKTGMIEQVAIENFKYMPFTQFANVIGVPAMSVPLHWCDSSSGNVGLPLGSQFVGGHGDEGKLL
ALAAQLEEAAPWFNKVPM

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=511, Percent_Identity=25.0489236790607, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI222831590, Length=530, Percent_Identity=24.5283018867925, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=318, Percent_Identity=31.1320754716981, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=514, Percent_Identity=26.8482490272374, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=30.6188925081433, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=30.6188925081433, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=251, Percent_Identity=31.0756972111554, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=235, Percent_Identity=29.3617021276596, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=162, Percent_Identity=35.8024691358025, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=24.124513618677, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=198, Percent_Identity=32.8282828282828, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922090, Length=198, Percent_Identity=32.8282828282828, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24652981, Length=198, Percent_Identity=32.8282828282828, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24652983, Length=198, Percent_Identity=32.8282828282828, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648441, Length=174, Percent_Identity=33.9080459770115, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648439, Length=174, Percent_Identity=33.9080459770115, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356731, Length=198, Percent_Identity=29.2929292929293, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53278; Mature: 53278

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAEYRQFDAIGLAELVARGEVTAMELLELAIARADAVNPQLNGLIIPLYDMARKRAAG
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCC
PLSGPLAGVPMLVKDLFQEIGGAPNYQGNKARKARDVHAQRDSTLVARWKQGGLVPFGRT
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
NTPEFGAKGVTEPDSFGPARNPWNVDHTPGGSSGGSAAMVAAGVVPVAGANDGGGSIRIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEECH
AACCGLFGLKPGRGRTPWGPEFTEAMHGIAVNHALTRSVRDSALLLDITQGDERGSLFQI
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEE
APPEQAYALSAASRPGKLRIGFSTRSPLGTDVDSEAIRAVEKTVALLRDLGHEVEEAEPQ
CCCCHHHEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
MDAKQMCMDWLSVWFGQCAAEVDEVKTMTGCGDEGFELDTLAIAAFGRATPAHEYVKCQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCHHHHEECC
RWQLYMIAMDTFLEQYDFWLTPTLASPPVGIGAMATPPWQQTALKVLLKVGGEKLLMKTG
CCEEEEHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHC
MIEQVAIENFKYMPFTQFANVIGVPAMSVPLHWCDSSSGNVGLPLGSQFVGGHGDEGKLL
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCEE
ALAAQLEEAAPWFNKVPM
HHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MKFAEYRQFDAIGLAELVARGEVTAMELLELAIARADAVNPQLNGLIIPLYDMARKRAAG
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCC
PLSGPLAGVPMLVKDLFQEIGGAPNYQGNKARKARDVHAQRDSTLVARWKQGGLVPFGRT
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
NTPEFGAKGVTEPDSFGPARNPWNVDHTPGGSSGGSAAMVAAGVVPVAGANDGGGSIRIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEECH
AACCGLFGLKPGRGRTPWGPEFTEAMHGIAVNHALTRSVRDSALLLDITQGDERGSLFQI
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEE
APPEQAYALSAASRPGKLRIGFSTRSPLGTDVDSEAIRAVEKTVALLRDLGHEVEEAEPQ
CCCCHHHEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
MDAKQMCMDWLSVWFGQCAAEVDEVKTMTGCGDEGFELDTLAIAAFGRATPAHEYVKCQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCHHHHEECC
RWQLYMIAMDTFLEQYDFWLTPTLASPPVGIGAMATPPWQQTALKVLLKVGGEKLLMKTG
CCEEEEHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHC
MIEQVAIENFKYMPFTQFANVIGVPAMSVPLHWCDSSSGNVGLPLGSQFVGGHGDEGKLL
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCEE
ALAAQLEEAAPWFNKVPM
HHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]