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The map label for this gene is priA [H]

Identifier: 110835102

GI number: 110835102

Start: 2559893

End: 2562067

Strand: Direct

Name: priA [H]

Synonym: ABO_2241

Alternate gene names: 110835102

Gene position: 2559893-2562067 (Clockwise)

Preceding gene: 110835099

Following gene: 110835103

Centisome position: 82.04

GC content: 61.61

Gene sequence:

>2175_bases
GTGTCATCCAAGAATACCCTGCAAATTGCCCTGCCCGTTCCCCTACCCGGGTGTTTTGACTATGCGCTGCACGGCACAGT
GATACCGCCCCGAGGGAGCCGAGTCAAAGTTCCCTTTGGGCGCCGCACCCTCATCGGCCTTGTCCACGGCCATGCTCCCA
GCGATTATCCAAAGCTGAAAGCTGTAGAGGCGGTGATCGACGAAGAGCCCGTTCTGCCCGATGAGCTTTACCAGCTCTGT
GAGCGGGCGGCCCGCTACTACCACCACCCTCTTGGGGAGGTGCTGAATTACGCACTCCCCACCTTGTTACGCCAAGGCGG
CGACGCCCAAGCCAGCCTGGAGAAACGCTGGCAGATTAGCGAACGGGGCCAGCATGTTAGCGATGACCAAGTTAGCCGCG
CCCCGCGCCAGCGAGACGCTTTGCGCATTTTGCGTGAGCATCCCCAAGGCCTGAGCAGCGCCATGCTAGCGGCACTGGAC
GTAAGCGCATCCGCCCTGAACAGCCTGCGCGAAAAGAGCTGGGCAAACCAAATTCACGTGGATACCCGCCCTGAGGTCCC
ACGAGAAACCCTGGCCCAGGCCCCGCTGGCCGCCAACGCCGAGCAGCGTGGGGCAATCCAGGCAATCCAGAAAGCCAAGG
GGTTCGCCCCCTTCCTGCTGGACGGCATCACCGGCAGCGGCAAAACCGAAGTCTACCTGCAAGCCATGGCGCCGCTGCTG
GCCGCCGGCAAACAGGTAATGGTGCTGGTTCCGGAAATCGGCCTGACCCCACAAACGGTACAGCGCTTCAAGCAGCGCTT
TGCCGTGCCTGTTGTCACGCTGCATTCTGGGCTCAGCGACCGAGAACGGCTGGACAACTGGCTCGCCGCCCGTGAGGGTC
ACGCGCGCATTATCATCGGCACTCGCTCAGCCATCTTCACGCCCATGCTGAACCCCGCTTTGATCATTGTGGACGAAGCC
CACGACAGCTCCCTCAAACAGCAAGATGGCTTCCGCTACCACGCGCGGGATCTGGCCACCTGGCGCGCCCAGCAGTTGGA
AATCCCGGTCATTCTGGGCAGCGCCACACCGGCACTGGAAACCCTGCATCTAACTAGGCAAGGCCGTTTCCAGTGGCTGA
AACTGTCCCAACGCGCCACCGCCCAGAGCCGCCCCCCCATCGAAATTGTTGATGCCCGCCTAGCCCCCCCCGGCAACCCA
CTACTGCCCCTCTCTTTGCAAGCGCTTAAACAATGCGTAGAGGCAGGTAATCAGGGGCTGGTGTTTATCAACCGCCGCGG
CTATGCCCCCATGATTCTCTGCCACGACTGTGGCTGGCAAGCAGAGTGCAAGCGTTGCGATAGCTTCCTCACCTGGCATC
GCAATGACCAGGCCCTGCGCTGCCATCATTGCGGCTATCAACAGCCGGTGCCTAGCCGCTGCCCGGATTGCGGCTCCAGC
GCCATTCAGGAAGCCGGTAGTGGCACCGAAAAGCTCACCGAGCTGCTTGAACAGTCCCTACCGGTACCGGTGATTCGCAT
CGACCGGGATGCCACTCGCCGCAAGGGCGCCTTAGATGCGCATCTGGCACAAATCCAAAAAGGCGAGCCCGCCGTCATGG
TCGGCACTCAGATGCTGGCCAAGGGCCACCATTTCCCCAAACTGACCTTGGTGGTGATCCTCGATATGGATTCTGGCTTT
CTCAGCGCCGACTTCCGCGGCCCTGAACAGGCCGCACAGTTGCTACTACAGGTGGCCGGCCGCAGCGGCCGCGAAGGCAA
AGGCCGGGTGATTCTGCAGAGCCGCCACCCGGATCATCACTTACTGCAACTAGCGGCCAGCGGCGACTACCCACAACTGG
CCACCACCCTGCTAGAAGAGCGACAGATGGCCGGACTGCCCCCCCACGGGCACTTGGCTCTGTTCCGCTGCGAAGCCATG
ACCATGGGCACCGCCATGAACTTTCTGCAACAGCTAAGCACTACTGCGGTTCCGCAGGATGTTCACGTCCTTGGCCCGAT
TCCCGCCCCCATGGAAAAACGTGCGGGCCGTTTCCGCACCCAGCTACTGCTGCAATGCAGCCAACGGGCGCCGTTACATC
AAGCCCTTAACGTGCTATTGGAACAGGCCCGCAAACTCCCTGAGGGGCGTCAATGCCGCTGGCACGTGGATGTGGATCCG
ATTGATATGTTATAG

Upstream 100 bases:

>100_bases
ACAGGCACCACACGGCTTGCGCCTGGGAAAAAGAAGGCGAATCATAACAGCTTGTCCCCATTGCGCAAGCGCAAAGCCGC
CGCGAGCTCCAGGAAATTCC

Downstream 100 bases:

>100_bases
CGACGCCGGAGACGGACGCCAGACAGAGCAGTGTCGGCCATCAGACATCCTGCGCATGCGTAAGCATGGCTTTTCCCGTA
CAATCGCCGGCCAGTTAAAC

Product: primosomal protein N`

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MSSKNTLQIALPVPLPGCFDYALHGTVIPPRGSRVKVPFGRRTLIGLVHGHAPSDYPKLKAVEAVIDEEPVLPDELYQLC
ERAARYYHHPLGEVLNYALPTLLRQGGDAQASLEKRWQISERGQHVSDDQVSRAPRQRDALRILREHPQGLSSAMLAALD
VSASALNSLREKSWANQIHVDTRPEVPRETLAQAPLAANAEQRGAIQAIQKAKGFAPFLLDGITGSGKTEVYLQAMAPLL
AAGKQVMVLVPEIGLTPQTVQRFKQRFAVPVVTLHSGLSDRERLDNWLAAREGHARIIIGTRSAIFTPMLNPALIIVDEA
HDSSLKQQDGFRYHARDLATWRAQQLEIPVILGSATPALETLHLTRQGRFQWLKLSQRATAQSRPPIEIVDARLAPPGNP
LLPLSLQALKQCVEAGNQGLVFINRRGYAPMILCHDCGWQAECKRCDSFLTWHRNDQALRCHHCGYQQPVPSRCPDCGSS
AIQEAGSGTEKLTELLEQSLPVPVIRIDRDATRRKGALDAHLAQIQKGEPAVMVGTQMLAKGHHFPKLTLVVILDMDSGF
LSADFRGPEQAAQLLLQVAGRSGREGKGRVILQSRHPDHHLLQLAASGDYPQLATTLLEERQMAGLPPHGHLALFRCEAM
TMGTAMNFLQQLSTTAVPQDVHVLGPIPAPMEKRAGRFRTQLLLQCSQRAPLHQALNVLLEQARKLPEGRQCRWHVDVDP
IDML

Sequences:

>Translated_724_residues
MSSKNTLQIALPVPLPGCFDYALHGTVIPPRGSRVKVPFGRRTLIGLVHGHAPSDYPKLKAVEAVIDEEPVLPDELYQLC
ERAARYYHHPLGEVLNYALPTLLRQGGDAQASLEKRWQISERGQHVSDDQVSRAPRQRDALRILREHPQGLSSAMLAALD
VSASALNSLREKSWANQIHVDTRPEVPRETLAQAPLAANAEQRGAIQAIQKAKGFAPFLLDGITGSGKTEVYLQAMAPLL
AAGKQVMVLVPEIGLTPQTVQRFKQRFAVPVVTLHSGLSDRERLDNWLAAREGHARIIIGTRSAIFTPMLNPALIIVDEA
HDSSLKQQDGFRYHARDLATWRAQQLEIPVILGSATPALETLHLTRQGRFQWLKLSQRATAQSRPPIEIVDARLAPPGNP
LLPLSLQALKQCVEAGNQGLVFINRRGYAPMILCHDCGWQAECKRCDSFLTWHRNDQALRCHHCGYQQPVPSRCPDCGSS
AIQEAGSGTEKLTELLEQSLPVPVIRIDRDATRRKGALDAHLAQIQKGEPAVMVGTQMLAKGHHFPKLTLVVILDMDSGF
LSADFRGPEQAAQLLLQVAGRSGREGKGRVILQSRHPDHHLLQLAASGDYPQLATTLLEERQMAGLPPHGHLALFRCEAM
TMGTAMNFLQQLSTTAVPQDVHVLGPIPAPMEKRAGRFRTQLLLQCSQRAPLHQALNVLLEQARKLPEGRQCRWHVDVDP
IDML
>Mature_723_residues
SSKNTLQIALPVPLPGCFDYALHGTVIPPRGSRVKVPFGRRTLIGLVHGHAPSDYPKLKAVEAVIDEEPVLPDELYQLCE
RAARYYHHPLGEVLNYALPTLLRQGGDAQASLEKRWQISERGQHVSDDQVSRAPRQRDALRILREHPQGLSSAMLAALDV
SASALNSLREKSWANQIHVDTRPEVPRETLAQAPLAANAEQRGAIQAIQKAKGFAPFLLDGITGSGKTEVYLQAMAPLLA
AGKQVMVLVPEIGLTPQTVQRFKQRFAVPVVTLHSGLSDRERLDNWLAAREGHARIIIGTRSAIFTPMLNPALIIVDEAH
DSSLKQQDGFRYHARDLATWRAQQLEIPVILGSATPALETLHLTRQGRFQWLKLSQRATAQSRPPIEIVDARLAPPGNPL
LPLSLQALKQCVEAGNQGLVFINRRGYAPMILCHDCGWQAECKRCDSFLTWHRNDQALRCHHCGYQQPVPSRCPDCGSSA
IQEAGSGTEKLTELLEQSLPVPVIRIDRDATRRKGALDAHLAQIQKGEPAVMVGTQMLAKGHHFPKLTLVVILDMDSGFL
SADFRGPEQAAQLLLQVAGRSGREGKGRVILQSRHPDHHLLQLAASGDYPQLATTLLEERQMAGLPPHGHLALFRCEAMT
MGTAMNFLQQLSTTAVPQDVHVLGPIPAPMEKRAGRFRTQLLLQCSQRAPLHQALNVLLEQARKLPEGRQCRWHVDVDPI
DML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=735, Percent_Identity=46.8027210884354, Blast_Score=615, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 79927; Mature: 79796

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKNTLQIALPVPLPGCFDYALHGTVIPPRGSRVKVPFGRRTLIGLVHGHAPSDYPKLK
CCCCCEEEEEECCCCCCHHHHHHCCEEECCCCCEEECCCCCCEEEEEEECCCCCCCCHHH
AVEAVIDEEPVLPDELYQLCERAARYYHHPLGEVLNYALPTLLRQGGDAQASLEKRWQIS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCHH
ERGQHVSDDQVSRAPRQRDALRILREHPQGLSSAMLAALDVSASALNSLREKSWANQIHV
HCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
DTRPEVPRETLAQAPLAANAEQRGAIQAIQKAKGFAPFLLDGITGSGKTEVYLQAMAPLL
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCHHHHHHHHHHHH
AAGKQVMVLVPEIGLTPQTVQRFKQRFAVPVVTLHSGLSDRERLDNWLAAREGHARIIIG
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
TRSAIFTPMLNPALIIVDEAHDSSLKQQDGFRYHARDLATWRAQQLEIPVILGSATPALE
CCHHHHHHCCCCEEEEEECCCCCCCHHHCCCEEHHHHHHHHHHHHEEEEEEECCCCCHHH
TLHLTRQGRFQWLKLSQRATAQSRPPIEIVDARLAPPGNPLLPLSLQALKQCVEAGNQGL
HHHHHHCCCCCEEEHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCE
VFINRRGYAPMILCHDCGWQAECKRCDSFLTWHRNDQALRCHHCGYQQPVPSRCPDCGSS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHH
AIQEAGSGTEKLTELLEQSLPVPVIRIDRDATRRKGALDAHLAQIQKGEPAVMVGTQMLA
HHHHCCCCHHHHHHHHHHCCCCCEEEECCHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHH
KGHHFPKLTLVVILDMDSGFLSADFRGPEQAAQLLLQVAGRSGREGKGRVILQSRHPDHH
CCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
LLQLAASGDYPQLATTLLEERQMAGLPPHGHLALFRCEAMTMGTAMNFLQQLSTTAVPQD
HEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCC
VHVLGPIPAPMEKRAGRFRTQLLLQCSQRAPLHQALNVLLEQARKLPEGRQCRWHVDVDP
CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCC
IDML
CCCC
>Mature Secondary Structure 
SSKNTLQIALPVPLPGCFDYALHGTVIPPRGSRVKVPFGRRTLIGLVHGHAPSDYPKLK
CCCCEEEEEECCCCCCHHHHHHCCEEECCCCCEEECCCCCCEEEEEEECCCCCCCCHHH
AVEAVIDEEPVLPDELYQLCERAARYYHHPLGEVLNYALPTLLRQGGDAQASLEKRWQIS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCHH
ERGQHVSDDQVSRAPRQRDALRILREHPQGLSSAMLAALDVSASALNSLREKSWANQIHV
HCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
DTRPEVPRETLAQAPLAANAEQRGAIQAIQKAKGFAPFLLDGITGSGKTEVYLQAMAPLL
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCHHHHHHHHHHHH
AAGKQVMVLVPEIGLTPQTVQRFKQRFAVPVVTLHSGLSDRERLDNWLAAREGHARIIIG
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
TRSAIFTPMLNPALIIVDEAHDSSLKQQDGFRYHARDLATWRAQQLEIPVILGSATPALE
CCHHHHHHCCCCEEEEEECCCCCCCHHHCCCEEHHHHHHHHHHHHEEEEEEECCCCCHHH
TLHLTRQGRFQWLKLSQRATAQSRPPIEIVDARLAPPGNPLLPLSLQALKQCVEAGNQGL
HHHHHHCCCCCEEEHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCE
VFINRRGYAPMILCHDCGWQAECKRCDSFLTWHRNDQALRCHHCGYQQPVPSRCPDCGSS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHH
AIQEAGSGTEKLTELLEQSLPVPVIRIDRDATRRKGALDAHLAQIQKGEPAVMVGTQMLA
HHHHCCCCHHHHHHHHHHCCCCCEEEECCHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHH
KGHHFPKLTLVVILDMDSGFLSADFRGPEQAAQLLLQVAGRSGREGKGRVILQSRHPDHH
CCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
LLQLAASGDYPQLATTLLEERQMAGLPPHGHLALFRCEAMTMGTAMNFLQQLSTTAVPQD
HEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCC
VHVLGPIPAPMEKRAGRFRTQLLLQCSQRAPLHQALNVLLEQARKLPEGRQCRWHVDVDP
CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCC
IDML
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]