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The map label for this gene is glpE [H]

Identifier: 110835073

GI number: 110835073

Start: 2525028

End: 2525843

Strand: Direct

Name: glpE [H]

Synonym: ABO_2212

Alternate gene names: 110835073

Gene position: 2525028-2525843 (Clockwise)

Preceding gene: 110835072

Following gene: 110835074

Centisome position: 80.93

GC content: 60.05

Gene sequence:

>816_bases
ATGCTGCCACTGCTGCTCGAACCCACCGAACTCGCCAACCGTCTTGACGACGGCAACCTGCTCATTCTCGATTTGAGCAA
ACAACCCGTTTACGTTCAGGCCCATGTTCCCGGCGCTATTCATCTGGATTTCAAGCGATTGCAGAGCGGCGCCCAGCCGG
CACCGGGGATGATCCCTTCCGATGATGCGCTGAGCGATCTTTTTTCTGAGCTGGGCCTGACCTCGGACACCCATGTGATC
GCCTATGACGATGAAGGTGGCGGTTGGGCTGGGCGCCTACTGTGGACCCTGGATGCCATCGGCCACAAGCACTACAGCTA
CCTGAACGGCGGCATCCACGCCTGGCTAGCGGACGGCCACGCCACCAGTAGCGAAGCCCACGAGCCCAGCCCCAGCGATT
ACCAGGTGGGCGCCCATGATCCGCTCACCGAAGTGGATCTGAACTACCTGCTTGCTCACTATCAGGATGAGGATGTGGTG
ATCTGGGATTCCCGCTCCCTGGACGAATTCGACGGCGTGCGCGCCTTCGCTAAAAAAGCCGGGCACATTCCCGGAGCTCG
CCACCACGAATGGACCGAGCCCATGGACAAGCAGAACAACCTGCGCTTGCGCCCACTGGACGACATCCGTGCTGAACTGG
CGGCTCTGGGCATCAGCGGCGACAAGGAAGTGATCACCCATTGCCAAACCCACCATCGATCCAGCTTTTCTTGGCTGGTG
GGCAAATTGCTAGGCTTTGAACGCATGCGTGGCTACGCCGGCTCCTGGGCGGAATGGGGCAACCACCCGGACACCCCTGC
AGAAAAAAGTGCCTAA

Upstream 100 bases:

>100_bases
ATAAGAAAGACCCCGGATAATGGATAATGAAGGCATTGCCGATACGAAGGCGCACGCCTTCGTCAATAATCATCAACTGC
TCACTGCCAACTGATCGCCT

Downstream 100 bases:

>100_bases
ACCCATCGTTGACGGCGGAGCCCCCTAGCGGCAATTTCCGCCTTCTGAAAAATGCTAAAGGACGTCACTCTTGAGCTTAC
GCGACAAACTGTTCGTTACC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLPLLLEPTELANRLDDGNLLILDLSKQPVYVQAHVPGAIHLDFKRLQSGAQPAPGMIPSDDALSDLFSELGLTSDTHVI
AYDDEGGGWAGRLLWTLDAIGHKHYSYLNGGIHAWLADGHATSSEAHEPSPSDYQVGAHDPLTEVDLNYLLAHYQDEDVV
IWDSRSLDEFDGVRAFAKKAGHIPGARHHEWTEPMDKQNNLRLRPLDDIRAELAALGISGDKEVITHCQTHHRSSFSWLV
GKLLGFERMRGYAGSWAEWGNHPDTPAEKSA

Sequences:

>Translated_271_residues
MLPLLLEPTELANRLDDGNLLILDLSKQPVYVQAHVPGAIHLDFKRLQSGAQPAPGMIPSDDALSDLFSELGLTSDTHVI
AYDDEGGGWAGRLLWTLDAIGHKHYSYLNGGIHAWLADGHATSSEAHEPSPSDYQVGAHDPLTEVDLNYLLAHYQDEDVV
IWDSRSLDEFDGVRAFAKKAGHIPGARHHEWTEPMDKQNNLRLRPLDDIRAELAALGISGDKEVITHCQTHHRSSFSWLV
GKLLGFERMRGYAGSWAEWGNHPDTPAEKSA
>Mature_271_residues
MLPLLLEPTELANRLDDGNLLILDLSKQPVYVQAHVPGAIHLDFKRLQSGAQPAPGMIPSDDALSDLFSELGLTSDTHVI
AYDDEGGGWAGRLLWTLDAIGHKHYSYLNGGIHAWLADGHATSSEAHEPSPSDYQVGAHDPLTEVDLNYLLAHYQDEDVV
IWDSRSLDEFDGVRAFAKKAGHIPGARHHEWTEPMDKQNNLRLRPLDDIRAELAALGISGDKEVITHCQTHHRSSFSWLV
GKLLGFERMRGYAGSWAEWGNHPDTPAEKSA

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=235, Percent_Identity=28.5106382978723, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI61835204, Length=234, Percent_Identity=26.4957264957265, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194473681, Length=234, Percent_Identity=26.4957264957265, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194473668, Length=234, Percent_Identity=26.4957264957265, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082121, Length=271, Percent_Identity=27.6752767527675, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081967, Length=250, Percent_Identity=26.8, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=254, Percent_Identity=22.0472440944882, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324825, Length=253, Percent_Identity=22.9249011857708, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29973; Mature: 29973

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLLLEPTELANRLDDGNLLILDLSKQPVYVQAHVPGAIHLDFKRLQSGAQPAPGMIPS
CCCCEECHHHHHHHCCCCCEEEEECCCCCEEEEEECCCEEEEEHHHHHCCCCCCCCCCCC
DDALSDLFSELGLTSDTHVIAYDDEGGGWAGRLLWTLDAIGHKHYSYLNGGIHAWLADGH
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCEEEEEECCC
ATSSEAHEPSPSDYQVGAHDPLTEVDLNYLLAHYQDEDVVIWDSRSLDEFDGVRAFAKKA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHH
GHIPGARHHEWTEPMDKQNNLRLRPLDDIRAELAALGISGDKEVITHCQTHHRSSFSWLV
CCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHH
GKLLGFERMRGYAGSWAEWGNHPDTPAEKSA
HHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLPLLLEPTELANRLDDGNLLILDLSKQPVYVQAHVPGAIHLDFKRLQSGAQPAPGMIPS
CCCCEECHHHHHHHCCCCCEEEEECCCCCEEEEEECCCEEEEEHHHHHCCCCCCCCCCCC
DDALSDLFSELGLTSDTHVIAYDDEGGGWAGRLLWTLDAIGHKHYSYLNGGIHAWLADGH
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCEEEEEECCC
ATSSEAHEPSPSDYQVGAHDPLTEVDLNYLLAHYQDEDVVIWDSRSLDEFDGVRAFAKKA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHH
GHIPGARHHEWTEPMDKQNNLRLRPLDDIRAELAALGISGDKEVITHCQTHHRSSFSWLV
CCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHH
GKLLGFERMRGYAGSWAEWGNHPDTPAEKSA
HHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]