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The map label for this gene is menE [H]

Identifier: 108812280

GI number: 108812280

Start: 2393677

End: 2395092

Strand: Reverse

Name: menE [H]

Synonym: YPN_2118

Alternate gene names: 108812280

Gene position: 2395092-2393677 (Counterclockwise)

Preceding gene: 108812281

Following gene: 108812279

Centisome position: 52.82

GC content: 57.7

Gene sequence:

>1416_bases
ATGGCATCGTTAGGCCATCCGCCTTGGGAGCATTGGCCTTGGCAGTATTGGGCCAATCTGCGGCCTCAAGCGCTCGCTAT
CCGTTCTGGGGGGCAACGGATCAGTTGGCAACAGTTAGCCAGTGATATCAATGCGTTGGCGGCTTTTTTTCAACAGCAAG
GTGTGGTGCCGGGGAGCGGCGTGGCCTTGCGCGGTAAAAACAGTTATACCCTGCTATTGGCTTATCTGGCGGCATTGCAG
TGTGCAGCTCGCGTTTTGCCGCTGAATCCGCAATTGCCGGATGCGCTTTTGGCGCAGCGCCTGCCAACGCTGGATATTCA
CTTTATGCTGAATTTAGCCACCCCGTTGCCCCCGCATTTTGCCTTTACTCCGTTGGATCCCCGCACCGTTGATTCGGCAG
CCGTGACCCCGATAGCGTGGGATGGCCAACGGCTGGCGACGCTGACGCTAACCTCAGGTTCCTCCGGCTTACCGAAGGCC
GCAGTACACTCGCTGGGCGCACATCGGGCTAATGCCGACGGCGTACTGGCGCTGATGAATTTCACCGCCAGTGATAGCTG
GTTGCTGTCGTTACCGTTGTTTCATGTCTCAGGGCAGGGGATTGTCTGGCGTTGGCTGACGGCGGGGGCGACACTGGGGG
TACCAGAGGGCGTGCCACTGGTTGAGGCTTTGGCGGGGTGCAGCCATGCGTCATTAGTTCCCACGCAATTATGGCGTTTG
TTGGCGAATGCCGCCCCCCCCTTGACGTTGAAAGAGGTACTGTTGGGGGGCGCGATGATCCCAACCGAACTGACCGAACA
GGCCGAGGCCCACGGCATCCGCTGTTGGTGTGGCTACGGCTTGACGGAGTTGGCATCAACGGTCTGTGCCAAGCGGGCAG
ACGGGCGACCTGGTGTCGGTGTCGCGCTCGCAGGCCGTCAGGTGAAACTGGTAGACGATGAAGTCTGGATAAAAGCCGAT
TGTCTGGCTGCCGGTTACTGGCAACAGGGGCAATTACTGCCACTCACGGACAGCGACGGGTGGTTCCATACCCGAGATCG
CGGTAGGTGGCAGCAAGGCGAATTATGTATTCTCGGGCGGTTGGATAATCTGTTTTTCAGTGGTGGCGAGGGGATCCAGC
CAGAAGACATCGAGCGAGTTTTATTGCAGTATTCAGGGGTGCAGCACGCTTTTGTTATTCCTGTTGCTGACGCTGAATTC
GGTCACCGGCCAGTGGCGGTCATTGATTCCGCTGATGAGGTGGATGTCGTGGCTTTAGCTGATTGGTTAGCCCCACGATT
GGCTGTCTTCCAGCGGCCCATTGCTTTTTACCGGCTACCCACATTTTTGAAGCGTGGCGGGATTAAGATCTCAAGGCAGC
AACTTGCCGACTGGCTGCGCGGCAAGTATGAAGGGGAGAAGATTGGCGAAAGATAG

Upstream 100 bases:

>100_bases
TTCCGGGGTTGGATACGCTGGACCTGATGCAGGCGCAAGTCGTGCGGCCGTGGCCAGACAGCCCGTTGCCGCTAATCACG
ACCGAGCAGTTGGGCGTGGT

Downstream 100 bases:

>100_bases
AGCATGTCAGGCAAACTCAGCCCAGTTTATTGTTAACGAGAGACCCATAGGGGGAAGGCCGCACCATGGATTACAGTAAA
ATTATCAAAGAGGTTGGGCG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGER

Sequences:

>Translated_471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGER
>Mature_470_residues
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSGVALRGKNSYTLLLAYLAALQC
AARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAA
VHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRLL
ANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKADC
LAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFG
HRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGER

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=55.2106430155211, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI145693145, Length=451, Percent_Identity=23.7250554323725, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=355, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786801, Length=437, Percent_Identity=25.4004576659039, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=488, Percent_Identity=27.0491803278689, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=22, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51177; Mature: 51046

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSG
CCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
EECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
DWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGER
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAAFFQQQGVVPGSG
CCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLDPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
EECCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVPEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LANAAPPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERVLLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
DWLAPRLAVFQRPIAFYRLPTFLKRGGIKISRQQLADWLRGKYEGEKIGER
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]