Click here to switch to the map view.

The map label for this gene is sppA [H]

Identifier: 108798015

GI number: 108798015

Start: 1139638

End: 1141419

Strand: Direct

Name: sppA [H]

Synonym: Mmcs_1042

Alternate gene names: 108798015

Gene position: 1139638-1141419 (Clockwise)

Preceding gene: 108798013

Following gene: 108798019

Centisome position: 19.97

GC content: 70.99

Gene sequence:

>1782_bases
ATGTTCGCCTTCCTACCCGGCATCCCCGGCGCCGACGATGTGCGCGCCCTGGTGCGACGGGTCGACACCGCACGCCACAA
GGGCATCCCCAACGGCTGCGTGCTCGAACTCGACCTCATGTCGGTGCCGCCGGAGACCAGCGGATTCGACCCGTTGGCGA
TGATCTCCGCCGGCGGGCGTCCCATGGTGCTCCGCCAGGTCGTCGCCGCCATCCACCGCGCCGCCGACGACGATCGCGTC
GCCGGTCTGATCGCGCGCGTGCAGATTTCCGCCGCGCCCGCCGCGCCGGTTCAAGAACTGAGGGAGGCCATCGCCGCCTT
CGGCGCGAAGAAGCCCTCGGTCGCCTGGGCCGAGACCTATCCCGGCACGCTGTCCTACTACCTGGCCTCGGCCTTCCGCG
AGGTGTGGATGCAGCCGTCCGGCACCGTCGGCCTCGTCGGCTTCGCCACCAGCGCCCTGTTCCTGCGCGATGCCCTCGAC
AAGGCCGGCATCCAGGCCCAGTTCACCGCGCGCGGCGAATACAAGTCGGCGGCAAACCTTTTCACGCAGGACAGCTACAC
CGAGCCCCATCGCGAAGCGGACAGCCGACTCATCGAGAGCCTGAACCAGCAGGTGCTCACCGCCGTCGCCGAATCCCGAA
AGCTGAACCCGGACGACATCGACGCCCTGGCCGACAAGGCGCCGCTGCTGCGTGACGCAGCGGTGGGCGGGGGTCTGGTC
GACCGCATCGGCTTCCGCGACGAGGCCTATGCCCGGGTCGCCGAACTCGCCGGCGCCGAAGGTGTTACGCCCCAGAACAC
CGACAGCCCCGACGCACCGCCGCGGCTGTTCCTGTCCCGCTACGCCCGCGCCACCGCGCCGGGTGGGGGCCCGTCGATCC
CCGGCCGCAAAGCCAAGCCCACCATCGCCGTCGTCACGCTGCACGGGCCGATCGTCAGCGGCCGCGGCGGTCCGGGTCTG
TCGCCCCTGGGCAATTCGAGCGCAGGCGGGGACACCATCACCGCCGCACTGCGCGAAGCCGCCGCCGACAAGGACGTCTC
GGCGATCGTGCTCCGGGTCGAGAGCCCCGGCGGATCGGTCACCGGTTCCGAGACCATCTGGCGCGAGGTGCTGCGCACCC
GCGAGGGCGGAACCCCCGTCGTCGCCTCCATGGGTGCGGTGGCCGCATCCGGCGGCTACTACGTCTCGATGGGTGCCGAC
GCCATCGTCGCCAACCCCGGCACCATCACCGGATCGATCGGCGTCGTCACCGGCAAGCTCGTGGCGCGCGAACTGAAGGA
CCGTCTCGGCGTCGGCTCGGACTCGGTGCGCACCAACGCCAATGCCGATGCCTGGTCGGCCAATTCGCCGTTCACCGACG
AACAGCAGGCGCATGTCGAGGCCGAGGCAGACCTGTTCTACACCGACTTCGTCGAACGCGTCGCCGACGGCCGTGGGCTG
TCCGTCGACGAGGTCGCCGAGGTGGCGCGGGGCCGGGTGTGGACCGGTGCCGACGCCAAGGACCGCGGACTGGTCGACGA
GCTCGGCGGCCTGCGCACCGCGATCGACCGGGCCAAGGTGCTCGCCGGCCTGGAGCCCGACACCGACGTCCGCATCGTCG
GCTACCCGGCGTCGTCGCTACTCGACCTGCTGCGCCCCAAACAGTCCTCACAACCCGCCGCGGCGTCGCTGCCCGATGCC
CTCGGGGTGCTCGTCGGCCGGTCGGTGGTCAGCCTGATCGACCAGGCCGAGCGGACCGTGAGCGGCGTCAACGCGATGTG
GCTGGGGGATTACCGGTTCTGA

Upstream 100 bases:

>100_bases
GCCGTCGGGATCGAACTGGGGGATCGAGATCGAGAACCGCACACCATCACGGTACGGCGGCCCCGCCGGCACACCCCCGC
GCGCCGGTACCCTCGGTCCT

Downstream 100 bases:

>100_bases
TACGTGCCGCTGACATAGACGATCTCGCCGAGCGGGTCGTTGTCGTGCGCCACCATCGGCACCCCGTGCTTGGCGAACAG
GTCCGTGCGCGGGAGGCCGG

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MFAFLPGIPGADDVRALVRRVDTARHKGIPNGCVLELDLMSVPPETSGFDPLAMISAGGRPMVLRQVVAAIHRAADDDRV
AGLIARVQISAAPAAPVQELREAIAAFGAKKPSVAWAETYPGTLSYYLASAFREVWMQPSGTVGLVGFATSALFLRDALD
KAGIQAQFTARGEYKSAANLFTQDSYTEPHREADSRLIESLNQQVLTAVAESRKLNPDDIDALADKAPLLRDAAVGGGLV
DRIGFRDEAYARVAELAGAEGVTPQNTDSPDAPPRLFLSRYARATAPGGGPSIPGRKAKPTIAVVTLHGPIVSGRGGPGL
SPLGNSSAGGDTITAALREAAADKDVSAIVLRVESPGGSVTGSETIWREVLRTREGGTPVVASMGAVAASGGYYVSMGAD
AIVANPGTITGSIGVVTGKLVARELKDRLGVGSDSVRTNANADAWSANSPFTDEQQAHVEAEADLFYTDFVERVADGRGL
SVDEVAEVARGRVWTGADAKDRGLVDELGGLRTAIDRAKVLAGLEPDTDVRIVGYPASSLLDLLRPKQSSQPAAASLPDA
LGVLVGRSVVSLIDQAERTVSGVNAMWLGDYRF

Sequences:

>Translated_593_residues
MFAFLPGIPGADDVRALVRRVDTARHKGIPNGCVLELDLMSVPPETSGFDPLAMISAGGRPMVLRQVVAAIHRAADDDRV
AGLIARVQISAAPAAPVQELREAIAAFGAKKPSVAWAETYPGTLSYYLASAFREVWMQPSGTVGLVGFATSALFLRDALD
KAGIQAQFTARGEYKSAANLFTQDSYTEPHREADSRLIESLNQQVLTAVAESRKLNPDDIDALADKAPLLRDAAVGGGLV
DRIGFRDEAYARVAELAGAEGVTPQNTDSPDAPPRLFLSRYARATAPGGGPSIPGRKAKPTIAVVTLHGPIVSGRGGPGL
SPLGNSSAGGDTITAALREAAADKDVSAIVLRVESPGGSVTGSETIWREVLRTREGGTPVVASMGAVAASGGYYVSMGAD
AIVANPGTITGSIGVVTGKLVARELKDRLGVGSDSVRTNANADAWSANSPFTDEQQAHVEAEADLFYTDFVERVADGRGL
SVDEVAEVARGRVWTGADAKDRGLVDELGGLRTAIDRAKVLAGLEPDTDVRIVGYPASSLLDLLRPKQSSQPAAASLPDA
LGVLVGRSVVSLIDQAERTVSGVNAMWLGDYRF
>Mature_593_residues
MFAFLPGIPGADDVRALVRRVDTARHKGIPNGCVLELDLMSVPPETSGFDPLAMISAGGRPMVLRQVVAAIHRAADDDRV
AGLIARVQISAAPAAPVQELREAIAAFGAKKPSVAWAETYPGTLSYYLASAFREVWMQPSGTVGLVGFATSALFLRDALD
KAGIQAQFTARGEYKSAANLFTQDSYTEPHREADSRLIESLNQQVLTAVAESRKLNPDDIDALADKAPLLRDAAVGGGLV
DRIGFRDEAYARVAELAGAEGVTPQNTDSPDAPPRLFLSRYARATAPGGGPSIPGRKAKPTIAVVTLHGPIVSGRGGPGL
SPLGNSSAGGDTITAALREAAADKDVSAIVLRVESPGGSVTGSETIWREVLRTREGGTPVVASMGAVAASGGYYVSMGAD
AIVANPGTITGSIGVVTGKLVARELKDRLGVGSDSVRTNANADAWSANSPFTDEQQAHVEAEADLFYTDFVERVADGRGL
SVDEVAEVARGRVWTGADAKDRGLVDELGGLRTAIDRAKVLAGLEPDTDVRIVGYPASSLLDLLRPKQSSQPAAASLPDA
LGVLVGRSVVSLIDQAERTVSGVNAMWLGDYRF

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=507, Percent_Identity=34.7140039447732, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787527, Length=187, Percent_Identity=33.6898395721925, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 61925; Mature: 61925

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFLPGIPGADDVRALVRRVDTARHKGIPNGCVLELDLMSVPPETSGFDPLAMISAGGR
CCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCC
PMVLRQVVAAIHRAADDDRVAGLIARVQISAAPAAPVQELREAIAAFGAKKPSVAWAETY
HHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCC
PGTLSYYLASAFREVWMQPSGTVGLVGFATSALFLRDALDKAGIQAQFTARGEYKSAANL
CCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
FTQDSYTEPHREADSRLIESLNQQVLTAVAESRKLNPDDIDALADKAPLLRDAAVGGGLV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCHH
DRIGFRDEAYARVAELAGAEGVTPQNTDSPDAPPRLFLSRYARATAPGGGPSIPGRKAKP
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
TIAVVTLHGPIVSGRGGPGLSPLGNSSAGGDTITAALREAAADKDVSAIVLRVESPGGSV
EEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
TGSETIWREVLRTREGGTPVVASMGAVAASGGYYVSMGADAIVANPGTITGSIGVVTGKL
CCHHHHHHHHHHHCCCCCCHHHHHCHHHCCCCEEEEECCCEEEECCCCEECCHHHHHHHH
VARELKDRLGVGSDSVRTNANADAWSANSPFTDEQQAHVEAEADLFYTDFVERVADGRGL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SVDEVAEVARGRVWTGADAKDRGLVDELGGLRTAIDRAKVLAGLEPDTDVRIVGYPASSL
CHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
LDLLRPKQSSQPAAASLPDALGVLVGRSVVSLIDQAERTVSGVNAMWLGDYRF
HHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MFAFLPGIPGADDVRALVRRVDTARHKGIPNGCVLELDLMSVPPETSGFDPLAMISAGGR
CCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCC
PMVLRQVVAAIHRAADDDRVAGLIARVQISAAPAAPVQELREAIAAFGAKKPSVAWAETY
HHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCC
PGTLSYYLASAFREVWMQPSGTVGLVGFATSALFLRDALDKAGIQAQFTARGEYKSAANL
CCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
FTQDSYTEPHREADSRLIESLNQQVLTAVAESRKLNPDDIDALADKAPLLRDAAVGGGLV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCHH
DRIGFRDEAYARVAELAGAEGVTPQNTDSPDAPPRLFLSRYARATAPGGGPSIPGRKAKP
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
TIAVVTLHGPIVSGRGGPGLSPLGNSSAGGDTITAALREAAADKDVSAIVLRVESPGGSV
EEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
TGSETIWREVLRTREGGTPVVASMGAVAASGGYYVSMGADAIVANPGTITGSIGVVTGKL
CCHHHHHHHHHHHCCCCCCHHHHHCHHHCCCCEEEEECCCEEEECCCCEECCHHHHHHHH
VARELKDRLGVGSDSVRTNANADAWSANSPFTDEQQAHVEAEADLFYTDFVERVADGRGL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SVDEVAEVARGRVWTGADAKDRGLVDELGGLRTAIDRAKVLAGLEPDTDVRIVGYPASSL
CHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
LDLLRPKQSSQPAAASLPDALGVLVGRSVVSLIDQAERTVSGVNAMWLGDYRF
HHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]